Resources on Halophilic.Haloalaklaiphilic microorganisms.Prof. S.P.Singh.June 2021 Exploration and studies of newer habitats, particularly extremes ones for environmental and Biotechnological applications Evolving and exploration of the microbial potential by molecular approaches such gene shuffling and Directed evolution Search for unique & novel biocatalytic capabilities for industrial & Environmental applications

1. Resources on Halophilic/Haloalkaliphilic
Microorganisms
Prof. Satya P. Singh, PhD
UGC-BSR Faculty Fellow (Professor-Emeritus) (Current)
UGC-CAS Department of Biosciences
Saurashtra University
RAJKOT-360 005. Gujarat, INDIA
Head, Department of Biosciences, Saurashtra University (2003-2020)
Coordinator, UGC-CAS Program, Saurashtra University (2013-2018)
Coordinator, DST-FIST Program (2007-2012)
Coordinator/PI, DBT-Multi Institutional Project (2007-2012)
PI, MoES-Multi Institutional Net Working Project (2014-2018)
Coordinator, M.Sc. Biotechnology, Saurashtra University (2004-2017)
Coordinator, UGC DSA-Phase-3 and UGC COCIST (2003-2006)
Email: satyapsingh@yahoo.com satyapsingh125@gmail.com spsingh@sauuni.ac.in
SU Website URL: saurashtrauniversity.edu/university/academic-departments/department-of-
biosciences/staff/dr-satya-prakash-singh/
ResearchGate: https://www.researchgate.net/profile/Satya_Singh5
GoogleScholar: https://scholar.google.com/citations?hl=en&user=jiAzOcgAAAAJ
UGC: https://vidwan.inflibnet.ac.in//profile/68903/Njg5MDM%3D

2. Limitations
Microbial activities only under limited set of
conditions
Only fraction (1-5%) of microbial world known
and explored Biocatalysts from microbes able to
function only under delicate & defined set of
Conditions
Applications under Natural Environmental
Conditions are limited

3. Need of the Hour
Exploration of newer habitats, particularly
extremes ones for environmental and
Biotechnological applications
Evolving the microbial potential by molecular
approaches such gene shuffling and Directed
evolution
Evolving unique & novel biocatalytic capabilities
for industrial & Environmental applications

4. Haloalkaliphilic bacteria
& Archaebacteria
Phylogeny, Diversity,
Enzymatic potential
Molecular Phylogeny
16S rRNA Sequencing
DNA-DNA Hybridization
Real Time PCR
Diversity Based on:
Morphology
Gram Reaction
Sugar Utilization
Enzyme secretion
Protein folding
Studies on Growth & Enzyme
Secretion as a function of:
Salt
PH
Nutritional factor
Purification& Characterization of
alkaline Protease
Effect of salt on pH and thermal
stability
Protein Denaturation & Renaturation
Cloning & Sequencing of
alkaline Protease
Salt & Regulation of
gene Expression
Metagenomics of
saline Habitats :
Phylogeny & Retrieval
of Novel genes for
biocatalysts

7. Production of Proteases

8. Production of Proteases

9. Extra cellular enzyme
detection
 Gohel, S. and Singh S.P. 2015. . International Journal of
Biological Macromolecules (IJBIOMAC). DOI:
10.1016/j.ijbiomac.2014.08.008, Vol 74: 421-429 (IF
3.00).
 Gohel, S. and Singh S.P. 2013. International Journal of
Biological Macromolecules (IJBIOMAC) 56: 20– 27 (IF
2.45).
 Gohel, S. and Singh S.P. 2012., J Chromatography-
B,889– 890, 61– 68 (IF 2.9).
 Gohel, S. and Singh S.P. 2012. International Journal of
Biological Macromolecules (IJBIOMAC) 50: 664– 671
(IF 2.45).

11. Characteristics of Proteases

15. Organisms Gene Bank Number Cultural characteristics
Optimum pH
(Range)
Optimum
Temperature (
Range)
Optimum
Salt (Range)
Solvent tolerance Reference
Haloalkaliphilic bacterium S-
15-9
GU059918
Round, opaque, smooth,
regular
9.5-10.5 60 5-25%
Methanol,
Propanol,Xylene,
n-hexane
Joshi RH 2006
Haloalkaliphilic bacterium S-
20-9
EU118360
Round, opaque, smooth,
regular
10.5 50-60 5-25%
Methanol,Butanol
Propanol,Xylene,
n-hexane
Joshi et al 2008
Haloalkaliphilic bacterium D-
15-9
HM047795
Round, opaque, smooth,
regular
10 50-60 5-25% Methanol, propanol Joshi RH 2006
Oceanobacillus oncorhynchi
D-20-91
HM047798
Round, opaque, smooth,
regular
9.5-12.5 50-60 5-25% -- Joshi RH 2006
Haloalkaliphilic bacterium
Ve2-10-10
HM047799
Round, opaque, smooth,
regular
10.5 50-60 5-25% -- Joshi RH 2006
Oceanobacillus oncorhynchi
Ve2-15-91
HM047796
Round, opaque, smooth,
regular
9.5-13 50-60 5-25%
Methanol, Butanol
Propanol,n-hexane
Joshi RH 2006
Oceanobacillus iheyensis Ve2-
20-92
HM047797
Round, opaque, smooth,
regular
10-13 50-60 5-25% -- Joshi RH 2006
Haloalkaliphilic bacteria Ve2-
20-91
HM047794
Round, opaque, smooth,
regular
8-10 50-90 5-25%
n-heptane, toluene,
benzene, chloroform, ethyl
acetate, isopropyl alcohol,
isoamyl alcohol
Raval et al 2014
Haloalkaliphilic bacteria AH6 EU118361
Round, opaque, smooth,
regular
8-13 50-60 0-4 M
methanol, propanpl,
hexane, heptanes,
isooctane, dodecanes,
decane and
cuclohexane.
Dodia MS 2005
Pandey and
Singh 2013
Oceanobacillus sp. SJ1 GQ162111
Round, opaque, smooth,
regular
10 50-60 5-25%
Methanol, Butanol
Propanol,n-hexane
Pandey et al
2012
Bacillus pseudofirmus SJ2 EU090232
Round, opaque, smooth,
10 50-60 5-25%
Methanol, Butanol
Dodia MS 2005
Enzymatic Characteristics of Haloalkaliphilic
bacteria

16. Structure & Function analysis of the
enzymes

18. ALKALINE PROTEASE: PRODUCTION AND
CATALYSIS UNDER NON-AQUEOUS
CONDITIONS

20. Organisms
NCBI
Accession
number
Gene cloned Host Reference
Haloalkaliphilic bacterium OME12 EU680960 Alkaline protease
BL21
(DE3)
Purohit and Singh
2013
Proc. Biochem
Metagenome from salt enriched
soil
--------------- Alkaline protease
BL21
(DE3)
Purohit and Singh
2013
IJBIOMAC
Oceanobacillus ihyehensis OME18 EU680961 Alkaline protease
BL21
(DE3)
Purohit and Singh
2013
Proc. Biochem
Alkalibacillus haloalkaliphilus C-5 --
Serine alkaline
protease
E.coli
(DH5α)
Rawal et al 2012
Haloakaliphilic bacteria Ve2-20-91 JX296114
Serine alkaline
protease
E.coli
(DH5α)
Raval et al. 2015
Ann Microbiology
Haloalkaliphilic actinomycetes
Serine alkaline
protease
BL21
(DE3)
Gohel & Singh,
2012
IJBIOMAC
Bacillus lehensis JO-26 ----------
Serine alkaline
protease
BL21
(DE3)
Hitarth Bhatt and S
P Singh 2019
( Unpublished)
Cloning, Expression and structure and function
relationship
of proteases from Haloalkaliphilic bacteria & Actinobacteria

22. Metagenomics & Non Cultivable

23. Metagenomics and Functional aspects

24. Book Chapters
2021
Rathore, D.S. Sheikh, M. and Singh, S. P. 2021. “Marine Actinobacteria: New Horizons in
Bioremediation”, P.425-449, in “Recent Developments in Microbial Technologies. Environmental and
Microbial Biotechnology, Edited by: Prasad R., Kumar V., Singh J., Upadhyaya C.P. Springer, Singapore.
https://doi.org/10.1007/978-981-15-4439-2_20, Print ISBN978-981-15-4438-5. Online ISBN978-981-
15-4439-2
2020
Nirajkumar, Sojitra, Singh, S. P. and Georrge J. and, 2020. “In Silico Identification of Drug Targets and
Drug-Like Molecules against Vibrio splendidus LGP32, Chapter-, P.401-414, in “ Marine Niche:
Applications in Pharmaceutical Sciences”, Edited by Nathani N.M., Mootapally C., Gadhvi I.R.,
Maitreya B., Joshi C.G., Springer, Singapore. https://doi.org/10.1007/978-981-15-5017-1_22, Print
ISBN 978-981-15-5016-4, Online ISBN 978-981-15-5017-1
2018
Gohel, S. D. Dangar, K.D., Sharma, A.K. Thakkar, F. and Singh, S. P., 2018. “Biology and applications
of extremophiles of thermophilic and alkaliphilic actenobacteria, in Chapter-6, P.103-136, in
“Extremophiles: From Biology to Biotechnology”, Edited by Subba Rao, CRC Press. Identifiers: LCCN
2017031972 | ISBN 9781498774925 (hardback), SBN: 9781498774932 (eBook)
Raval, V.H., Bhatt, H. B. and Singh, S. P., 2018. “Adaptation strategies in halophilic bacteria, Chapter-7,
P.137-164, in “Extremophiles: From Biology to Biotechnology”, Edited by Subba Rao, CRC Press.
Identifiers: LCCN 2017031972 | ISBN 9781498774925 (hardback), SBN: 9781498774932 (eBook)

25. 2017
Dangar, K. G., Raiyani, N.M., Pandya, R.D. and Singh, S.P. 2017.Uncultivated Lineages and Host–
Microbe Interaction in Saline Environment, Chapter-2, P.13-28, in “Understanding Host-Microbiome
Interactions- An Omics Approach”, Edited by Singh, R.P., Koringa, P.G., Kothari, R.K. and Singh, S.P.
Springer. ISBN 978-981-10-5049-7, ISBN 978-981-10-5050-3 (eBook), DOI 10.1007/978-981-10-5050-3,
Library of Congress Control Number: 2017952944
Rakholiya, K. D., Kaneria, M.J., Singh, S.P., Vora, V.D. and Sutaria, G.S. 2017. Biochemical and
Proteomics Analysis of the Plant Growth-Promoting Rhizobacteria in Stress Conditions, Chapter-14, P.227-
246, in “Understanding Host-Microbiome Interactions- An Omics Approach”, Edited by Singh, R.P.,
Koringa, P.G., Kothari, R.K. and Singh, S.P. Springer. ISBN 978-981-10-5049-7, ISBN 978-981-10-5050-
3 (eBook), DOI 10.1007/978-981-10-5050-3, Library of Congress Control Number: 2017952944
Gohel, S.D., Sharma, A.K., Thakrar, F.J. and Singh, S.P. 2017. Endophytic Actinobacteria and Their
Interactions with Plant Host Systems, Chapter-15, P.247-266, in “Understanding Host-Microbiome
Interactions- An Omics Approach”, Edited by Singh, R.P., Koringa, P.G., Kothari, R.K. and Singh, S.P.
Springer. ISBN 978-981-10-5049-7, ISBN 978-981-10-5050-3 (eBook), DOI 10.1007/978-981-10-5050-3,
Library of Congress Control Number: 2017952944

26. Recent Developments in Microbial
Technologies pp 425-449| 2020 Cite as
Marine Actinobacteria: New Horizons
in Bioremediation
•Dalip Singh Rathore, Mahejbin Sheikh,
Satya P. Singh
•Department of BiosciencesUGC Centre
Advanced Studies (CAS), Saurashtra
Rajkot, India

29. Bio-databases designed

30. Recent Publications: Prof. Satya P. Singh
( Cumulative Impact factor : 201, H-Index: 31)
2021
Dwivedi, Purna, Sharma, A. K. and Singh, S.P. 2021. Biochemical properties and repression
studies of an alkaline serine protease from a haloalkaliphilic actinomycete, Nocarpdiopsis
dassonvillei subsp. albirubida OK-14. Biocatalysis and Agricultural Biotechnology,
Accepted. 07 June 2021 (Elsevier; IF: 0.90)
Kikani, B.A. and Singh, S.P. 2021. Amylases from thermophilic bacteria: Structure and
Function Relationship. Critical Reviews in Biotechnology, In Press, 30 April 2021 (Taylor &
Francis; IF: 8.102)
Rathore, D. R., Sheikh, M., Gohel G.D, and Singh, S.P. 2021. Genetic and phenotypic
heterogeneity of the Nocardiopsis alba strains of sea water. Current Microbiology, 78: 1377-
1387 (Springer; IF: 1.75), DOI: 10.1007/s00284-021-02420-0
•

31. 2021
•
Chauhan, J.V., Mathukiya, R. Singh, S.P. and Gohel, S.D. 2021. Two steps purification,
biochemical characterization, thermodynamics and structure elucidation of thermostable
alkaline serine protease from Nocardiopsis alba strain OM-5. International Journal of
Biological Macromolecules (IJBIOMAC), 169: 39-50 (Elsevier; IF: 5.16),
https://doi.org/10.1016/j.ijbiomac.2020.12.061 , Available On-Line 12 Dec 2020.
Rathore, D R and Singh, S.P. 2021. Kinetics of growth and co-production of amylase and
protease in novel marine actinomycete, Streptomyces lopnurensis KaM5. Folia
Microbiologica (Springer; IF: 1.70), https://doi.org/10.1007/s12223-020-00843-z

32. Recent Publications
( Cumulative Impact factor : 201, H-Index: 31)
2020
Sharma, A.K. Kikani, B.A. and Singh S.P. 2020, Diversity and Phylogeny of Actinomycetes of
Arabian Sea along the Gujarat Coast. Geomicrobiology Journal (Taylor & Francis, IF 1.90),
DOI: 10.1080/01490451.2020.1860165
Raiyani, Nirali and Singh S.P. 2020, Extraction of environmental DNA, construction of
metagenomic libraries and functional screening of enzymes from salt pan soil, Indian Journal
of Geo-Marine Sciences, Accepted (NISCARE, CSIR, IF 0.50),
Raiyani, Nirali and Singh S.P. 2020, Taxonomic and functional profiling of the microbial
communities of Arabian Sea: A Metagenomics approach
Journal: Genomics (Elsevier, IF 6.20), 112:4361- 4369
https://doi.org/10.1016/j.ygeno.2020.07.024
Bhatt, H.B. and Singh S.P. 2020, Cloning, Expression and structural elucidation of a
biotechnologically potential alkaline serine protease from a newly isolated Haloalkaliphilic
Bacillus lehensis JO-26, Frontiers in Microbiology (IF 4.25), 11:1-16,
https://doi.org/10.3389/fmicb.2020.00941

33. 2020
Sharma, A.K. Kikani, B.A. and Singh S.P. 2020, Biochemical, thermodynamic and structural
characteristics of a biotechnologically compatible alkaline protease from a haloalkaliphilic,
Nocardiopsis dassonvillei OK-18 International Journal of Biological Macromolecules
(IJBIOMAC), 153:680-696, DOI: 10.1016/j.ijbiomac.2020.03.006 (IF 5.16)
Pandya, Rupal D. and Singh S.P. 2020, Pigment production by an extreme halophilic archaeon
on Halorubrum sp. J4.2.2 from little Rann of Kutch, Gujarat, India. Research Journal of
Biotechnology, 15(1):88-100. E-ISSN: 2278-4535 Print ISSN: 0973-6263

34. 2019
Thakrar, F.J. and Singh S.P. 2019. Catalytic, thermodynamic and structural properties of an
immobilized and highly thermostable alkaline protease from a haloalkaliphilic actinobacteria,
Nocardiopsis alba Tata-5. Bioresource Technology, 278:150-158 (IF 5.802)
Sheikh, M., Rathore, D.S., Gohel, S.D. and Singh S.P. 2019. Cultivation and characteristics of
the Marine Actinobacterial from the Sea water of Alang, Bhavnagar. Indian Journal of Geo-
Marine Sciences (CSIR-NISCARE), 48(12), 1896-1901(IF 0.4).
Rathore, D.S., Sheikh, M.A., Gohel, S.D. and Singh, S.P. (2019) Isolation strategies, abundance
and characteristics of the marine actinomycetes of Kachhighadi, Gujarat, India. Journal of
Marine Biological Association of India (JMBAI), CMFRI Cochin, India 61(1): 21-27

35. 2018
Gohel, S. D. and Singh S.P. 2018. Thermodynamics of a Ca2+ dependent, highly thermostable
and detergent compatible purified alkaline serine protease from Nocardiopsis xinjiangensis
strain OM-6. International Journal of Biological Macromolecules (IJBIOMAC),
https://doi.org/10.1016/j.ijbiomac.2018.02.157, 113:565-574 (IF 3.00)
Gohel, S. D. and Singh S.P. 2018. Molecular phylogeny and diversity of the salt-tolerant
alkaliphilic actinobacteria inhabiting Coastal Gujarat, India. Geomicrobiology
Journal, DOI: 10.1080/01490451.2018.1471107, 35:9, 775-789 (IF 1.5)
Thakrar, F.J., Kikani, B.A., Sharma, A.K. and Singh S.P. 2018. Stability of alkaline proteases
from haloalkaliphilic actinobacteria probed by circular dichroism spectroscopy. Applied
Biochemistry and Microbiology (Russia), 54(6), 591-602 (IF 0.68)
Sheikh, M., Rathore, D. S., Gohel, S. D. and Singh S.P. 2018. Marine actinobacteria associated
with the invertebrate hosts: a rich source of bioactive compounds: A Review. (Invited
contribution) Journal of Cell &Tissue Research, 18 (01), 6361-6374.

36. 2018
Dangar, K. G., Kalasava, A. B., Dave, A. V. and Singh S.P. 2018. Molecular diversity of
Nocardiopsis alba sp. isolated from the coastal region of Gujarat, India. Journal of Cell
&Tissue Research, 18(3) 6559-6570
Vaidya A., Nair, V. S., Georrge, J. and Singh S.P. 2018. Comparative analysis of
thermophilic proteases, Research Journal of Life Sciences, Bioinformatics, Pharaceutical
and Chemical Sciences (RJLBPCS) 4(6), P. 66. DOI: 10.26479/2018.0406.05
Pandey, S. Sharma, A.K., Solanki, Kiran P. and Singh S.P. January 2018. Catalysis and
stability of an extracellular α- amylase from a haloalkaliphilic bacterium as a function of the
organic solvents at different pH, salt concentrations and temperatures. Indian Journal of
Geo-Marine Sciences (CSIR-NISCARE), 47 (01), 240-248 (IF 0.4).

37. 2017
Bhatt, H.B., Gohel, S.D. and Singh, S.P. 2017. Phylogeny, Novel bacterial lineage and
enzymatic potential of haloalkaliphilic bacteria from the saline coastal desert of Little Rann of
Kutch, Gujarat, India. 3 Biotech, 8,53, https://doi.org/10.1007/s13205-017-1075-0 (IF 1.36)
Bhatt, H.B., Begum, M.A., Chintalapati, S., Chintalapati, V.R. and Singh, S.P.
2017. Desertibacillus haloalkaliphilus gen. nov.sp. nov., isolated from a salt desert.
International Journal of Systematic & Evolutionary Microbiology (IJSEM), 67(11):4435-
4442 (IF 2.1)
Kikani, B.A., Sharma, A.K. & Singh, S.P. 2017. Metagenomic and Culture-Dependent
Analysis of the Bacterial Diversity of a Hot Spring Reservoir as a Function of the Seasonal
Variation. International Journal of Environmental Research, 11: 25-38.
DOI:10.1007/s41742-017-0003-9 (IF 1.0).
Datta, A., Sharma, A., Kundu, R.S. and Singh S.P. 2017. Diversity and enzymatic profile of
bacterial flora in the gut of an estuarine fish, Mugil jerdoni. Indian Journal of Geo-Marine
Sciences (CSIR-NISCARE), 46(06): 1116-1127 (IF 0.4)

38. 2016
Sharma, A.K. and Singh S.P. 2016. Effect of amino acids on the repression of alkaline protease
synthesis in haloalkaliphilic Nocardiopsis dassonvillei Biotechnology Reports (Elsevier),
12:40-51 DOI: http://dx.doi.org/10.1016/j.btre.2016.10.004 (IF 0.5).
Shukla R. and Singh S.P. 2016. Structural and catalytic properties of immobilized α- amylase
from Laceyella sacchari TSI-2. International Journal of Biological Macromolecules
(IJBIOMAC), 85, 208-216 (IF 3.00).
Thakrar F.J., Gohel S.D. and Singh S.P. (2016) Repression of the synthesis of alkaline
proteases of salt-tolerant alkaliphilic actinomycetes” Asian Journal of Biochemical and
Pharmaceutical Research (AJBPR) 6 (4).

39. 2015
1. International Journal of Biological Macromolecules (IJBIOMAC). (In Press) (IF
3.00).
2. Process Biochemistry, 50 (12), 2128–2136 (doi:10.1016/j.procbio.2015.10.013)
(IF 2.63).
3. Indian Journal of Geo-Marine Sciences (CSIR-NISCARE) (In Press) (IF 0.4).
4. International Journal of Biological Macromolecules (IJBIOMAC). 81,450-460
(doi: 10.1016/j.ijbiomac.2015.08.032) (IF 3.00).
5. Microbiology (Mscow, Russia), 84 (6), 00-00 (IF 0.80)
6. Starch/Stärke, 67,1 –11 (IF 1.40)
7. International Journal of Biological Macromolecules (IJBIOMAC). DOI:
10.1016/j.ijbiomac.2014.08.008, Vol 74: 421-429 (IF 3.00)
2014
1.Environmental Monitoring and Assessment. 186:6351–6362 (IF 1.6)
2. GDNA Mitochondrial DNA. (IF 1.8)
3. Process Biochemistry 49: 61-68, (IF 2.63)
4. Annals of Microbiology, (IF 1.5). doi:10.​1007/​s13213-014-0869-0
5. Process Biochemistry 49 (6): 955-962 (IF 2.63)

40. 2013
1. IJBIOMAC) 56: 20– 27 (IF 3.00)
2. Genome Announcements (ASM, USA) 1(2):
3. IJBIOMAC, 56: 20– 27 (IF 3.00)
4. Bioprocess and Biosystems Engineering – BPBSE 36(5): 567-577 (IF 1.81)
2012
1. Journal of Bacteriology, J. Bacteriol. 194(17): 4775 (IF 3.82)
2. Bioinformation, 8 (11):535-538 (IF 1.12)
3. Process Biochemistry, 47: 1791-1798 (IF 2.627)
4. Journal of Bioscience and Bioengineering, 114 (3), 251-256 (IF 1.8)
5. Appl. Biochemistry and Biotechnology, 166:1747-1757. (IF 1.94)
6. J Chromatography-B,889– 890, 61– 68 (IF 2.9)
7. IJBIOMAC 50: 664– 671 (IF 3.00)
8. Braz. J Microbiol 43 (4): 1595-1603 (IF 0.65)

41. 2011
1. (IJBIOMAC), 49:103–112 (IF 3.00)
2. New Biotechnology 28(2):IF 2.76)
3. Biotechnology Bioprocess Engineering, 16, 6:1180-1186 (IF 1.28)
4.IJBIOMAC 48: 676–681 (IF 3.00)
5. J Scientific & Industrial Research, CSIR70(11): 976-981 (IF 0.51)
6. Journal of Bioremediation and Biodegradation, 2-1. (IF 1.20)
7. Journal of Bioremediation and Biodegradation, 2-2. (IF 1.20)
2010
1. Biotechnology Bioprocess Engineering
2. Journal of Industrial Microbiology & Biotechnology (JIMB)
3. Journal of Industrial Microbiology & Biotechnology (JIMB)

42. Dr. Bharat Joshi (Canada)
Dr. Manish Bhatt ( Canada)
Dr. Rajesh K. Patel ( Patan)
Dr. Anju Mittal ( USA)
Dr. Mital Dodia ( Canada)
 Dr.. Jignasha Thumar
( Gandhinagar)
Dr. Rupal Joshi (ZRC, Ahmedabad)
Dr. Chetna Rajyaguru ( Rajkot)
Ms. Geera Mankad ( Rajkot)
Dr. Chirantan Raval ( Asst Prof., GovtCollege)
 Dr. Megha Purohit ( Scientist, Canada)
Dr. Himanshu Bhimani
(Associate Prof, Navsari Ag Univ)
Dr. Bhavtosh Kikani
(Asstt Prof. CHARUSAT)
Dr. Vikram Raval (Asstt Prof. Gujarat University)
Dr. Sangeeta Gohel (Asstt Prof. Saurashtra University)
Dr. Sandeep Pandey (Scientist, Pharma, Daman)
Dr. Viral Akbari ( Scientist, UK)
Dr. Rushit Shukla (Asstt Prof. Christ College, Rajkot)
Dr. Amit Sharma (Scientist, ZRC, Ahmedabad)
Dr. Kruti Dangar (Asstt Prof. Saurashtra University)
Dr. Atman Vaidya ( Biology Teacher & Entrepreneur)
Mr. Hitarth Bhatt (Asstt Prof. Virani College, Rajkot)
Ms. Rupal Pandya (USA)
Ms. Foram Thakrar
Acknowledgements : Ph.D. Students

43. Financial Support
DBT, UGC, DST, MoES, GSBTM,
Saurashtra University, Rajkot
Research Collaborations
•IIT Delhi, New Delhi: Prof. S. K.Khare
•DUSC, New Delhi: Prof. Sanjay Kapoor
•NFRI, Tsukuba, Japan: Dr. Kiyoshi Hayashi ( Now at
Toyo University, Japan)
•Griffith University, Australia
•JNTU Hyderabad, Prof. Ch. Sasikala
•Central University of Hyderabad, Prof. Ch. Rama Rao