The document summarizes a research project that aims to investigate the influence of resveratrol, epsilon-viniferin, piceatannol and xanthohumol on neurodegenerative processes and their role in regulating sirtuin 1 (SIRT1) activity. The methodology involves building a cellular model of Alzheimer's disease and determining the effects of the polyphenols on SIRT1 catalytic activity, amyloid β levels, and other markers. The research is expected to provide useful insights into the protective mechanisms of the compounds and potentially lead to applications in therapies by optimizing SIRT1 activity.

1. Joanna Gerszon
The influence of resveratrol, epsilon-viniferin,
piceatannol and xanthohumol on neurodegeneratives
processes. The role of chosen polyphenols in regulation
sirtuin 1 activity

2. The research purpose
if there are any relations
between effects of chosen
natural compounds and
SIRT1 activity in neuronal
cells

3. Methodology, step by step…
 to build a cellular model of AD, I stably transfected human hippocampal neurons with Swedish
mutated gene APPSwe.
 in order to determine the conditions of the experiments I conducted serious of dose-response
assays using neutral red, which allowed me to determine the cytotoxicity of polyphenols studied in
relation to control cells and transfected with Swedish mutated gene APPSwe.
To accomplish main research aims I determined catalytic activity of SIRT1 and changes in its
expression.
SIRT1 is involved in the activation of multiple pathways, associated with neurodegenerative processes,
thus I will assess:
 level of amyloid β,
 level of NEP (an enzyme involved in promoting degradation of amyloid beta-peptide),
 level of BDNF (brain-derived neurotrophic factor, which is involved in mechanisms of synaptic
plasticity in the adult brain)
 level of NF-kB, which controls global pro-inflammatory response
The activity of superoxide dismutase, catalase and glutathione reductase will be also determined. The
examination of antioxidant properties of polyphenols will be based on assessment of chosen
parameters for cells treated and untreated with oxidizing factor (ionizing radiation). The percentage
of apoptotic and necrotic cells will be also estimated.

4. Research project impact
 Proposed comprehensive analysis will be useful in explaining the mechanism of protective
influence of chosen compounds. The conducted experiments will make it possible to e.g.
compare efficiency of polyphenols in neuronal cells different the amount of released Aβ,
 Beside its purely cognitive aspects, it can have a substantial practical value in terms of the
possible application. Introduction of compounds responsible for catalytic activity of sirtuin 1,
will speed up therapies optimization due to application of relatively cheap and safety
biofarmaceutics,
 Gaining insight into mechanism of effects of resveratrol, epsilon-viniferin, piceatannol and
xanthohumol on neuronal cells will also indicate the direction of research aiming at the
development of the new strategies for prevention of age related diseases.