This is our poster of primary outcomes in our research project that has been presented on the 3rd National Festival & International Congress on Stem Cell & Regenerative Medicine congress. The full text of this project was published in the journal of Reproductive Biology and Endocrinology with the title of "Intra-ovarian injection of platelet-rich plasma into ovarian tissue promoted rejuvenation in the rat model of premature ovarian insufficiency and restored ovulation rate via angiogenesis modulation". King regards

1. Shahin Ahmadian1, Sepideh Sheshpari2, Mohammad Pazhang1, Rahim Beheshti3, Mehran Mesgari Abbasi4, Mohammad Nouri5,6, Reza Rahbarghazi5,7, Mahdi Mahdipour5,6*
1. Department of Biology, Faculty of Science, Azarbaijan Shahid Madani University, Tabriz, Iran
2. Department of Midwifery, Faculty of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran
3. Department of Veterinary Science, Islamic Azad University Shabestar Branch, Shabestar, Iran
4. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
6. Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
7. Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
Email: mahdipourm@tbzmed.ac.ir
Platelet rich plasma and its restorative effect on fertility for rat model of menopause;
A pre-clinical study
Background and Aim:
• Due to natural physiological aging, women at age 50-52 years old, face
menopause and its related obstacle including infertility.
• Early menopause occurs in about 1% of women under 40 and 0.1% under 30
years in general population.
• Several causes have been identified for early menopause such as iatrogenic
causes (mostly because of oncology treatment), idiopathic, genetic and
autoimmune diseases.
• Several therapies have been proposed in this regard, however, the
effectiveness of them remains to be further illustrated.
.
Methods:
• In this study, we examined the restorative effect of Platelet-rich plasma
(PRP) as a natural blood product in the rat model of menopause.
• The animal models were generated within 15 days, using a daily injection of
the gonadotoxic agent, 4-Vinylcyclohexene Diepoxide. The control group
received saline in the whole period of animal modeling.
• After confirmation of menopause by histological studies, a group of rats was
injected (intra-ovarian injection) by freshly prepared rat PRP in two
concentrations of 1 and 4X (15 rat/ group).
• Other two groups were either received saline or did not get any injection.
• Two months were assigned for evaluation of the treatment, in which every
two weeks, 3 rats from each group randomly were allocated for histological
assessment of ovarian tissue.
• At the end stage, reproductive performance of 3 rats per group were
assessed.
Results:
• Preliminary results indicated partially restoration of ovarian function in the
groups of rats received PRP in both concentrations.
• Histological studies revealed that PRP treatment improved the follicle
numbers compared to the non-treated counterpart.
• The most follicle counts were observed in PRP 1X and 4X groups 4 and 6
weeks post-treatment respectively, whereas numerous atretic follicles were
noticed in the saline, sham and VCD groups.
• Considering reproductive performance of the rats, no significant differences
were detected between groups in birth rates.
Conclusion:
• Our results illustrated that PRP could be applied as an alternative therapeutic
agent for the treatment of patients at early stages of menopause or about to
enter into this complication such as cancer patients who are candidate to
gonadotoxic chemotherapy and radiotherapy.
• Quite optimistic, but further experimentations should be performed to
approve the safety and applicability of the technique in the human setup.
Keywords:
• Menopause; Infertility; Platelet-Rich Plasma; Animal Model; Ovarian
rejuvenation
VCD
injection
first
day
VCD
injection
final
day
Intra-ovary
injection
First
sampling
point
Second
sampling
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Third
Sampling
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Final
sampling
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Mating
trail
Offspring
monitoring
1 15 16 30 44 58 72 73 ~96
Day
Intra-ovary injection Offspring
Healthy antral follicle Atretic antral follicle