Poster C Linical Nk Generation Ash 2009 Js
•Download as PPT, PDF•
0 likes•180 views
1. A clinical protocol was designed to test the safety and efficacy of using ex vivo generated natural killer (NK) cells from umbilical cord blood CD34+ cells as a donor lymphocyte infusion for acute myeloid leukemia patients. 2. The Glycostem basal growth medium was used to efficiently generate large numbers of functional NK cells devoid of T-cells and B-cells for clinical use. 3. In phase I/II trials, escalating doses of autologous NK cells will be infused in KIR-ligand mismatched acute myeloid leukemia patients to evaluate toxicity.
Report
Share
Report
Share
![Adoptive transfer of CD34+ derived NK cells in poor-prognosis AML patients ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Effective NK cell differentiation from umbilical cord blood (UCB) derived CD34+ cells using GBGM ® E x-vivo generated NK cells are devoid of T- and B cells and express specific NK cell antigens Stroma-free cell culture system for optimal NK cell generation ,[object Object],[object Object],[object Object],[object Object],[object Object],Clinical grade NK cell cultures provide sufficient numbers of functional NK cells which efficiently lyse various primary AML tumor cells Efficient CD34+ cell enrichment from cryopreserved UCB units using the CliniMACS system Efficient NK cell production from cryopreserved UCB units using various bioreactors CD56 content 99% ± 1% using GBGM ® 10 100 1,000 10,000 100,000 1 2 3 4 5 week Fold expansion total cells Medium1 (n=3) Medium 2 (n=3) GBGM ® (n=3) Percentage CD56+ cells A B * 0 20 40 60 80 100 3 4 5 week * Medium1 (n=3) Medium 2 (n=3) Cell expansion ~50,000 fold using GBGM ® expansion differentiation 1 • GBGM ® + human serum (HS) • SCF, Flt3L, IL-7, TPO • Clinical grade Heparin (GAGs) CD34+ cells Day 0 - 9 Day 9 - 14 Expansion medium: Differentiation medium 2: • SCF, IL-7, IL-2, IL-15 NK progenitors Mature NK cells STEP 1 STEP 2 differentiation 2 Day 14 - 35 Differentiation medium 1: • SCF, IL-7, Flt3L, IL-15 • Clinical grade Heparin (GAGs) Low - dose cytokine cocktail • Low - dose cytokine cocktail • Low - dose cytokine cocktail • • GBGM ® + HS • GBGM ® + HS Novel GMP grade Glycostem Basal Growth Medium (GBGM ® ) www.glycostem.nl Clinical scale generation of allogeneic NK cell products 0 500 1000 1500 2000 0 1 2 3 4 5 6 week CB0109 bag CB0209 bag CB0309 bag 0 20 40 60 80 100 0 1 2 3 4 5 6 week CB0109 plate CB0209 plate CB0309 plate 0 20 40 60 80 100 0 1 2 3 4 5 6 week CB0109 bag CB0209 bag CB0309 bag 0 2000 4000 6000 8000 0 1 2 3 4 5 6 week CB0109 plate CB0209 plate CB0309 plate 0 2000 4000 6000 0 1 2 3 4 5 6 week CB0709 wave CB0709 plate 0 20 40 60 80 100 0 1 2 3 4 5 6 week CB0709 wave CB0709 plate Fold expansion Fold expansion Fold expansion NK cells (%) NK cells (%) NK cells (%) 0 0 5 95 0 0 9 91 0 4 96 0 CD3 CD19 CD15 SSC CD14 CD38 CD56 CD45 0 20 40 60 80 100 AML1 AML2 AML3 AML4 AML5 K562 KG1a day2 day3 day1 specific lysis (%) NK cell products are analyzed for purity, showing no contamination with T- or B cells (A). The NK cell phenotype was determined by 10-color flowcytometry using the Gallios Flow Cytometer and the Kaluza software (B). A B The final NK cell products were analyzed for functionality in a CFSE based cytotoxicity assay for 18h with a 2:1 effector:target ratio. Control experiments in plates Experiments in static bags Experiments in bioreactors GBGM ® (n=3) NK cells per experiment CD34+ cells fold expansion CD56+ (%) NK cells experiment CB0109 1.7x10 6 1770 63 1.9x10 9 static bag CB0209 1.4x10 6 759 80 8.6x10 8 static bag CB0309 1.3x10 6 1291 70 1.2x10 9 static bag CB0709 0,81x10 6 2861 95 2.2x10 9 bioreactor](https://image.slidesharecdn.com/posterclinicalnkgenerationash2009js-12598251094189-phpapp01/85/Poster-C-Linical-Nk-Generation-Ash-2009-Js-1-320.jpg)
Recommended
Freiburg Nk2009 Jan Spanholtz
The document summarizes research on using natural killer (NK) cells for immunotherapy against acute myeloid leukemia (AML). It describes past studies showing adoptive transfer of haploidentical NK cells can lead to transient NK cell engraftment and in vivo expansion in some AML patients. However, these studies had limitations like low cell numbers, T-cell contamination, and lack of standardization. The document then describes the development of an expansion and differentiation technology using a novel growth medium (GBGM) to efficiently generate large numbers of highly cytotoxic NK cells from cord blood, bone marrow, or mobilized peripheral blood derived CD34+ cells. It discusses several pre-clinical and early clinical studies using these ex vivo expanded NK cells for
Effects of CTGF Overexpression on Fibrosis-Related Phenotypes
Wound healing consists of three phases: inflammation, proliferation, and remodeling. During proliferation and remodeling, new extracellular matrix (ECM) is laid down by cells called fibroblasts. ECM consists of proteins that provide structural support in tissues. ECM production and fibroblast migration and proliferation are stimulated by connective tissue growth factor (CTGF). During wound healing, CTGF is induced by transforming growth factor beta (TGF-beta) to stimulate this response. When TGF-beta and CTGF are overexpressed, excessive amounts of ECM proteins are laid down, forming scar tissue, also known as fibrosis. Fibrotic tissue in organs can impair healthy function, leading to organ failure. To better understand how CTGF functions in fibrotic tissue, cells with varying levels of CTGF expression will be examined in a cell model of wounding. We will measure gene expression and observe cells for changes in fibrosis-related phenotypes. We hypothesize that increased CTGF expression will lead to enhanced cell growth, motility, and ECM production, as compared to controls. Results will provide a foundation for further research into the effects of CTGF variations on fibrosis-related phenotypes.
regulatory t cells and gvhd
This document discusses using ex vivo expanded and fucosylated regulatory T cells (Tregs) from third party umbilical cord blood to prevent graft-versus-host disease (GVHD) after transplantation. Experiments in mice show that fucosylated Tregs home more effectively to sites of inflammation, persist longer in vivo, and reduce GVHD symptoms and mortality more than untreated Tregs. The document proposes a clinical trial to evaluate the safety and efficacy of fucosylated Tregs for preventing GVHD in patients receiving double cord blood transplants.
3.24.2010
The document summarizes key concepts about the cell cycle, including:
1) The cell cycle consists of interphase (G1, S, G2 phases) and mitosis (M phase), with checkpoints between phases to ensure cell readiness.
2) Experiments fused cells at different phases and observed activation, identifying maturation promoting factor (MPF) as important for mitosis.
3) Further work identified MPF as a cyclin-Cdk complex, with cyclin levels and Cdk activating phosphorylations regulating progression through the cell cycle.
4) The cyclin B-Cdk1 complex promotes mitosis, driving events like nuclear envelope breakdown, before cyclin degradation inactivates Cdk1
Continuous engagement of self specific activation receptor induces nk tolerance
This study investigated the effects of persistent engagement of the activating NK cell receptor Ly49H with its ligand m157. The results showed that transgenic expression of m157 in mice led to downregulation of Ly49H receptor expression on NK cells and impaired Ly49H+ NK cell function. Specifically, Ly49H+ NK cells from m157 transgenic mice produced less IFN-γ upon stimulation. However, adoptive transfer experiments demonstrated the defect in Ly49H+ NK cells was reversible and did not depend on the level of Ly49H expression. Continuous engagement of Ly49H with m157 was necessary to induce Ly49H dysfunction, providing evidence that persistent receptor stimulation can impair NK cell responses.
FGFBP1 pathways control after induction of a conditional transgene in a mouse...
A systems biology approach to analyzing large data sets, such as this study which involved five full mouse cDNA arrays allows the researcher to capture a snapshot of the unfolding remodeling events of an organisms response to change, stress or disease. Analyzing data in this form involves filtering the biological signal from the noise. Sorting the noise in appropriate manners is essential to be able to complete the biological story. Building on existing knowledge base, we can complete the picture as long as the proper context of the collection, normalization and analysis is maintained. High throughput technologies such as microarrays and RNA sequencing as enabled by next generation sequencing presents the researcher with the challenge of extracting meaningful information from the measurements. Software tools and analysis techniques are not a substitute to understanding the biological context from which the data are collected. Engineering and digital signal processing has allowed us to derive the understanding of how to reconstruct a signal from the presence of a continual stream of noisy analog data. Sampling frequency and proper filtering are a must to be able to sort out a meaningful signal from the noise. These same principles apply not only to communication theory but also when studying large data such as those that may be collected from high throughput systems such as a Affymetrix mouse cDNA array.
Sezary syndrome part 2
This document summarizes information about natural killer (NK) cells, mRNA vaccines, T-cell receptors, and NK cell-based cancer immunotherapy. It includes definitions and background information on NK cells, how they kill cancer cells, and their roles in the innate immune system and COVID-19. It also discusses types of NK cells, components of humoral immunity, cytokines, and NK cell cancer immunotherapy approaches including ex vivo expansion, genetic engineering, and clinical trials. Finally, it provides information on specific NK cell therapies including IPH4102/Lacutamab for cutaneous T-cell lymphoma and discusses challenges and strategies to improve NK cell persistence and antitumor activity.
1-s2.0-S0014480015000970-main
1) Betacellulin (BTC) transgenic mice were studied to examine the effects of constitutive BTC overexpression on the urinary bladder.
2) BTC was detected in microvascular structures and umbrella cells of the uroepithelium lining. ERBB1 and ERBB4 receptors were also present in the uroepithelium.
3) BTC transgenic mice and mice that were double transgenic for BTC and a dominant kinase-dead EGFR mutant developed hyperplasia of the uroepithelium at 5 months of age, suggesting BTC signaling was not solely dependent on ERBB1/EGFR.
Recommended
Freiburg Nk2009 Jan Spanholtz
The document summarizes research on using natural killer (NK) cells for immunotherapy against acute myeloid leukemia (AML). It describes past studies showing adoptive transfer of haploidentical NK cells can lead to transient NK cell engraftment and in vivo expansion in some AML patients. However, these studies had limitations like low cell numbers, T-cell contamination, and lack of standardization. The document then describes the development of an expansion and differentiation technology using a novel growth medium (GBGM) to efficiently generate large numbers of highly cytotoxic NK cells from cord blood, bone marrow, or mobilized peripheral blood derived CD34+ cells. It discusses several pre-clinical and early clinical studies using these ex vivo expanded NK cells for
Effects of CTGF Overexpression on Fibrosis-Related Phenotypes
Wound healing consists of three phases: inflammation, proliferation, and remodeling. During proliferation and remodeling, new extracellular matrix (ECM) is laid down by cells called fibroblasts. ECM consists of proteins that provide structural support in tissues. ECM production and fibroblast migration and proliferation are stimulated by connective tissue growth factor (CTGF). During wound healing, CTGF is induced by transforming growth factor beta (TGF-beta) to stimulate this response. When TGF-beta and CTGF are overexpressed, excessive amounts of ECM proteins are laid down, forming scar tissue, also known as fibrosis. Fibrotic tissue in organs can impair healthy function, leading to organ failure. To better understand how CTGF functions in fibrotic tissue, cells with varying levels of CTGF expression will be examined in a cell model of wounding. We will measure gene expression and observe cells for changes in fibrosis-related phenotypes. We hypothesize that increased CTGF expression will lead to enhanced cell growth, motility, and ECM production, as compared to controls. Results will provide a foundation for further research into the effects of CTGF variations on fibrosis-related phenotypes.
regulatory t cells and gvhd
This document discusses using ex vivo expanded and fucosylated regulatory T cells (Tregs) from third party umbilical cord blood to prevent graft-versus-host disease (GVHD) after transplantation. Experiments in mice show that fucosylated Tregs home more effectively to sites of inflammation, persist longer in vivo, and reduce GVHD symptoms and mortality more than untreated Tregs. The document proposes a clinical trial to evaluate the safety and efficacy of fucosylated Tregs for preventing GVHD in patients receiving double cord blood transplants.
3.24.2010
The document summarizes key concepts about the cell cycle, including:
1) The cell cycle consists of interphase (G1, S, G2 phases) and mitosis (M phase), with checkpoints between phases to ensure cell readiness.
2) Experiments fused cells at different phases and observed activation, identifying maturation promoting factor (MPF) as important for mitosis.
3) Further work identified MPF as a cyclin-Cdk complex, with cyclin levels and Cdk activating phosphorylations regulating progression through the cell cycle.
4) The cyclin B-Cdk1 complex promotes mitosis, driving events like nuclear envelope breakdown, before cyclin degradation inactivates Cdk1
Continuous engagement of self specific activation receptor induces nk tolerance
This study investigated the effects of persistent engagement of the activating NK cell receptor Ly49H with its ligand m157. The results showed that transgenic expression of m157 in mice led to downregulation of Ly49H receptor expression on NK cells and impaired Ly49H+ NK cell function. Specifically, Ly49H+ NK cells from m157 transgenic mice produced less IFN-γ upon stimulation. However, adoptive transfer experiments demonstrated the defect in Ly49H+ NK cells was reversible and did not depend on the level of Ly49H expression. Continuous engagement of Ly49H with m157 was necessary to induce Ly49H dysfunction, providing evidence that persistent receptor stimulation can impair NK cell responses.
FGFBP1 pathways control after induction of a conditional transgene in a mouse...
A systems biology approach to analyzing large data sets, such as this study which involved five full mouse cDNA arrays allows the researcher to capture a snapshot of the unfolding remodeling events of an organisms response to change, stress or disease. Analyzing data in this form involves filtering the biological signal from the noise. Sorting the noise in appropriate manners is essential to be able to complete the biological story. Building on existing knowledge base, we can complete the picture as long as the proper context of the collection, normalization and analysis is maintained. High throughput technologies such as microarrays and RNA sequencing as enabled by next generation sequencing presents the researcher with the challenge of extracting meaningful information from the measurements. Software tools and analysis techniques are not a substitute to understanding the biological context from which the data are collected. Engineering and digital signal processing has allowed us to derive the understanding of how to reconstruct a signal from the presence of a continual stream of noisy analog data. Sampling frequency and proper filtering are a must to be able to sort out a meaningful signal from the noise. These same principles apply not only to communication theory but also when studying large data such as those that may be collected from high throughput systems such as a Affymetrix mouse cDNA array.
Sezary syndrome part 2
This document summarizes information about natural killer (NK) cells, mRNA vaccines, T-cell receptors, and NK cell-based cancer immunotherapy. It includes definitions and background information on NK cells, how they kill cancer cells, and their roles in the innate immune system and COVID-19. It also discusses types of NK cells, components of humoral immunity, cytokines, and NK cell cancer immunotherapy approaches including ex vivo expansion, genetic engineering, and clinical trials. Finally, it provides information on specific NK cell therapies including IPH4102/Lacutamab for cutaneous T-cell lymphoma and discusses challenges and strategies to improve NK cell persistence and antitumor activity.
1-s2.0-S0014480015000970-main
1) Betacellulin (BTC) transgenic mice were studied to examine the effects of constitutive BTC overexpression on the urinary bladder.
2) BTC was detected in microvascular structures and umbrella cells of the uroepithelium lining. ERBB1 and ERBB4 receptors were also present in the uroepithelium.
3) BTC transgenic mice and mice that were double transgenic for BTC and a dominant kinase-dead EGFR mutant developed hyperplasia of the uroepithelium at 5 months of age, suggesting BTC signaling was not solely dependent on ERBB1/EGFR.
virchowsarch
This document summarizes research on immortalizing bovine pancreatic duct cells and making them tumorigenic through genetic manipulation. Key points:
1) Bovine pancreatic duct cells were immortalized by transfecting them with SV40 large T antigen cDNA. The immortalized cells retained features of normal duct cells.
2) The immortalized duct cells were then transfected with a mutated K-ras gene. This caused the cells to gain the ability to form tumors when injected into nude mice, suggesting K-ras plays an important role in pancreatic carcinogenesis.
3) The research aims to establish a model for the transition from normal duct cell to neoplastic cell in order to study the initial steps of pancreatic cancer development
presentation-Honors 2013
The document summarizes a research project that aimed to develop a stable cell line that constitutively expresses the nucleocapsid protein of Crimean-Congo hemorrhagic fever virus (CCHFV). The student transfected baby hamster kidney cells with a construct containing the CCHFV nucleocapsid protein gene and screened for stable transfectants using G418 selection. Transfection efficiency was highest with Lipofectamine 2000. Immunofluorescence assays detected nucleocapsid protein expression. Further work is needed to confirm protein expression by SDS-PAGE and generate a stable cell line for use in CCHFV diagnostics.
Agarikon.1 and Agarikon Plus Affect Cell Cycle and Induce Apoptosis in Human ...
This study investigated the effects of two medicinal mushroom extract blends, Agarikon.1 and Agarikon Plus, on two human tumor cell lines, H460 and Caco-2. The extracts were found to inhibit the growth of both cell lines in a concentration-dependent manner and induce cell cycle perturbations by delaying progression through the G1 and S phases. While a modest induction of early and late apoptosis was observed, no caspase-3 cleavage was detected. The results indicate that the extracts have antiproliferative and mainly cytostatic activity on the tumor cell lines through cell cycle disturbances and increases in p53 and p21 protein expression.
UCSBigemPosterSmaller
The document describes an engineered contact-dependent inhibition system to induce gene expression upon contact between bacteria. Overlap extension PCR was used to generate a CysK-T25 fusion plasmid which was ligated into a resistance backbone. I-T18 was also cloned and ligated separately. A background cell line containing a GFP reporter downstream of the Pap operon promoter was constructed. The engineered plasmids were then transformed into this cell line. Upon contact with a bacteria expressing the cognate toxin, the system aims to trigger GFP expression by bringing the CysK and I-T18 fusions into proximity to form active adenylate cyclase, producing cAMP and activating the Pap promoter. This demonstrates a potential approach for contact
cancerres
This study found that transforming growth factor beta 1 (TGF-β1) induces desmoplasia, the proliferation of fibrotic tissue, in experimental models of human pancreatic carcinoma. The researchers transfected pancreatic tumor cells (PANC-1) that do not normally induce desmoplasia or express TGF-β1 with the TGF-β1 gene. They found that the TGF-β1-transfected cells gained the ability to induce fibroblast growth in culture and in vivo in nude mice. This effect was due both to the direct effects of TGF-β1 and its upregulation of other growth factors and matrix proteins. Therefore, TGF-β1 plays an important role in the desm
cms_042247
The document summarizes research evaluating the performance of TaqMan Low Density Arrays for gene expression analysis. It finds that assays on arrays can discriminate 2-fold changes as well as plate assays, and assay results are highly reproducible both within and across arrays. The study also uses a TaqMan Low Density Human Endogenous Control Array to examine housekeeping gene expression across 32 tissues, identifying genes with consistent expression levels suitable for normalization.
NK-cell therapy for Hematologic Malignancies. Prof. Akihiro Shimosaka
This document summarizes Akihiro Shimosaka's credentials and research on expanding natural killer (NK) cells for cancer therapy. It provides details on various methods for expanding NK cells from sources like peripheral blood mononuclear cells (PBMCs) and umbilical cord blood. The methods are compared in terms of fold expansion, cytotoxicity of expanded NK cells, and expression of activating receptors. Clinical cases demonstrate that NK cell therapy can improve outcomes for cancer patients. The document also discusses using expanded NK cells for antibody-dependent cellular cytotoxicity cancer treatment and their potential in allogeneic stem cell transplantation.
The use of reverse genetics to facilitate the growth of FMDV for the product...
The European Commission for the Control of Foot-and-Mouth Disease (EuFMD), one of FAO’s oldest Commissions, came into being on the 12th June 1954, with the pledge of the sixth founding member state to the principles of a coordinated and common action against Foot-and-mouth Disease.
To integrate promoter-GW-lux fragment onto chromosome of E.coli
This document describes integrating a promoter-lux fragment onto the chromosome of E. coli to provide more robust results in promoter assays compared to a plasmid. The lux fragment was amplified by PCR and ligated into the destination vector pGRG25. This new plasmid, pGRG25-pDEW201GWlux, was transformed into E. coli. The promoter sequence was then integrated onto the chromosome using Gateway Technology. Promoter activity was tested under different conditions to evaluate strain backgrounds and DNA damage.
Rennert et al. ASH 2019 talk
Aleta Biotherapeutics biologic bridging protein for the treatment of patients relapsing from CAR-CD19 therapies, presented at #ASH19
3768 rennert
1) CAR T cells specific for CD19 have shown remarkable success in treating blood cancers but have had limited effect on solid tumors which lack the CD19 antigen.
2) The IMPACT technology uses fusion proteins containing the extracellular domain of CD19 linked to single-chain antibodies targeting other antigens to bridge CAR19 T cells to solid tumors.
3) Experiments showed CAR19 T cells redirected by a CD19-anti-HER2 fusion protein were cytotoxic against HER2-positive ovarian cancer cells in vitro, demonstrating the IMPACT technology can redirect CAR T cells to new tumor targets through antigen bridging.
ALETA CNS metastasis program 8 July 2020
Slide deck used for video presentation to the OCTS conference. Her2-positive CNS metastases plague refractory breast cancer patients. Here we present our wholly novel approach to cell therapy for the treatment of these patients. #celltherapy #oncology #breastcancer
oncogene
1) NF-kB is constitutively activated in pancreatic cancer cells but not normal pancreatic cells, suggesting it plays a role in pancreatic tumorigenesis.
2) Expressing a mutant IkBa (IkBaM) that inhibits NF-kB suppressed the tumorigenicity of pancreatic cancer cells in a mouse model.
3) Inhibiting NF-kB reduced expression of pro-survival genes like Bcl-xL and Bcl-2, and reduced VEGF and IL-8 expression which are involved in angiogenesis. This suggests NF-kB inhibition can suppress tumorigenesis by reducing pro-survival and angiogenic factors.
Eddie Senior Seminar! 1
The document summarizes research on expressing HIV-1 antigens in plants as a potential vaccine. The researchers found that transient expression in plants is a viable alternative to transgenic plants. They determined that targeting the HIV-1 Gag protein Pr55Gag and its derivative p17/24 to chloroplasts yielded the highest levels of these proteins. Mice vaccinated with p17/24 produced from chloroplasts developed strong CD4+ and CD8+ T cell responses and HIV-specific antibodies, showing it is a promising vaccine candidate.
Real-time PCR method for detection of the transgenic rice event TT51-1
This document summarizes a research article that developed and validated real-time PCR methods for detecting the transgenic rice event TT51-1. The researchers established both qualitative and quantitative PCR assays to distinguish TT51-1 from other rice varieties. Validation experiments demonstrated the methods could accurately detect down to 10 template copies of the TT51-1 gene. The authors hope this validated detection method can help regulate and control TT51-1 rice.
Detection and Surveillance of Antibiotic Resistance Genes From Food and Ferti...
One potential way to acquire antibiotic resistance genes is through the food supply chain. Both livestock and feed may
acquire antibiotic resistant bacteria via different mechanisms. Foodstuffs can be exposed to antibiotic resistant bacteria
through fertilizer originating from waste-water treatment plants. This, in addition to increasing administration of antibiotics
to livestock, can lead to food being a potential source of antibiotic resistance genes. This may lead to horizontal gene
transfer to pathogenic enteropathogens and further to drug resistance in humans. Therefore, the surveillance and prevention
of antibiotic resistance genes in food is important.
To effectively combat the spread of difficult-to-treat bacterial infections, rapid surveillance methods to detect antibiotic
resistance genes are required; in order to monitor both bacterial isolates and metagenomic samples.
Since the gut is known to act as a reservoir for antibiotic resistance genes, a small-scale research study was performed on
5 stool samples isolated from healthy human adults using an antibiotic resistance gene identification PCR array. In addition,
the diversity of antibiotic resistance genes in municipal biosolids was determined using an Antibiotic Resistance Genes
Microbial DNA qPCR Array with DNA extracted from belt-filter, press-cake sewage samples.
22 antibiotic resistance genes were identified from different resistance classifications. Further studies were performed in
beef, chicken, vegetable and pork samples. In conclusion, PCR arrays can be effective tools for detection of antibiotic
resistance genes from food samples and potential fertilizer sources.
Comparison of Culture Media for In Vitro T Cell Expansion & Function
Presented by Astarte Biologics (astartebio.com) at the Society for Immunotherapy of Cancer (SITC) 33rd Annual Meeting. This research explores a series of studies comparing the performance of several culture media.
AACR poster VP_2014
This document describes research into developing prostate cancer immunotherapy using chimeric antigen receptor (CAR) T cells. The researchers generated two CAR constructs - CAR-PSMA containing an antibody targeting prostate-specific membrane antigen (PSMA), and CAR-NKG2D containing the human NKG2D receptor. They transduced human T cells with these constructs, which resulted in surface expression of the tumor-targeting receptors. In vitro experiments demonstrated the CAR T cells specifically bound and killed prostate cancer cells expressing the target antigens, indicating the potential for an effective CAR T cell immunotherapy for prostate cancer.
journal.ppat.1005708 (1)
This research article examines how activation of natural killer (NK) cells impacts susceptibility to Listeria monocytogenes (Lm) infection. The study finds that depletion of NK cells significantly reduces bacterial burdens and increases survival in infected mice, indicating NK cells increase host susceptibility to Lm infection. This effect is independent of early interferon-gamma (IFNγ) production by NK cells, as depletion of NK cells still protected IFNγ receptor-deficient mice. The article further investigates how a bacterial virulence protein, p60, drives NK cells to switch from producing IFNγ to producing the regulatory cytokine IL-10, which suppresses myeloid cell responses and increases bacterial burdens.
Enhanced NK cell adoptive antitumor effects against breast cancer in vitro
This is the research paper which i have been choosen for presentation "Enhance NK cell adoptive antitumor effects against breast cancer in vitro via blockade of the Transforming Growth Factor-Beta".
T cell and NK-cell neoplasms
This document discusses T-cell and NK-cell neoplasms. It begins by describing NK cells and their morphology as large granular lymphocytes that lack T-cell receptors and CD3 expression but express CD56 and CD16. The document then covers the various types of T-cell and NK-cell neoplasms including their morphology, immunophenotype, and genetics. Key neoplasms discussed are T-cell lymphoblastic leukemia/lymphoma, T-cell prolymphocytic leukemia, T-cell large granular lymphocytic leukemia, and extranodal NK/T-cell lymphoma among others. The document provides details on the diagnostic criteria for each type of neoplasm.
CAR-T (Cell Therapy) Nomenclature Review & Brand Equity Study. April 15, 2015
CAR-T (Cell Therapy) Nomenclature Review & Brand Equity Study. April 15, 2015.
Brand Acumen. The Global Leader in Pharmaceutical Name Development and Submission Strategy.
More Related Content
What's hot
virchowsarch
This document summarizes research on immortalizing bovine pancreatic duct cells and making them tumorigenic through genetic manipulation. Key points:
1) Bovine pancreatic duct cells were immortalized by transfecting them with SV40 large T antigen cDNA. The immortalized cells retained features of normal duct cells.
2) The immortalized duct cells were then transfected with a mutated K-ras gene. This caused the cells to gain the ability to form tumors when injected into nude mice, suggesting K-ras plays an important role in pancreatic carcinogenesis.
3) The research aims to establish a model for the transition from normal duct cell to neoplastic cell in order to study the initial steps of pancreatic cancer development
presentation-Honors 2013
The document summarizes a research project that aimed to develop a stable cell line that constitutively expresses the nucleocapsid protein of Crimean-Congo hemorrhagic fever virus (CCHFV). The student transfected baby hamster kidney cells with a construct containing the CCHFV nucleocapsid protein gene and screened for stable transfectants using G418 selection. Transfection efficiency was highest with Lipofectamine 2000. Immunofluorescence assays detected nucleocapsid protein expression. Further work is needed to confirm protein expression by SDS-PAGE and generate a stable cell line for use in CCHFV diagnostics.
Agarikon.1 and Agarikon Plus Affect Cell Cycle and Induce Apoptosis in Human ...
This study investigated the effects of two medicinal mushroom extract blends, Agarikon.1 and Agarikon Plus, on two human tumor cell lines, H460 and Caco-2. The extracts were found to inhibit the growth of both cell lines in a concentration-dependent manner and induce cell cycle perturbations by delaying progression through the G1 and S phases. While a modest induction of early and late apoptosis was observed, no caspase-3 cleavage was detected. The results indicate that the extracts have antiproliferative and mainly cytostatic activity on the tumor cell lines through cell cycle disturbances and increases in p53 and p21 protein expression.
UCSBigemPosterSmaller
The document describes an engineered contact-dependent inhibition system to induce gene expression upon contact between bacteria. Overlap extension PCR was used to generate a CysK-T25 fusion plasmid which was ligated into a resistance backbone. I-T18 was also cloned and ligated separately. A background cell line containing a GFP reporter downstream of the Pap operon promoter was constructed. The engineered plasmids were then transformed into this cell line. Upon contact with a bacteria expressing the cognate toxin, the system aims to trigger GFP expression by bringing the CysK and I-T18 fusions into proximity to form active adenylate cyclase, producing cAMP and activating the Pap promoter. This demonstrates a potential approach for contact
cancerres
This study found that transforming growth factor beta 1 (TGF-β1) induces desmoplasia, the proliferation of fibrotic tissue, in experimental models of human pancreatic carcinoma. The researchers transfected pancreatic tumor cells (PANC-1) that do not normally induce desmoplasia or express TGF-β1 with the TGF-β1 gene. They found that the TGF-β1-transfected cells gained the ability to induce fibroblast growth in culture and in vivo in nude mice. This effect was due both to the direct effects of TGF-β1 and its upregulation of other growth factors and matrix proteins. Therefore, TGF-β1 plays an important role in the desm
cms_042247
The document summarizes research evaluating the performance of TaqMan Low Density Arrays for gene expression analysis. It finds that assays on arrays can discriminate 2-fold changes as well as plate assays, and assay results are highly reproducible both within and across arrays. The study also uses a TaqMan Low Density Human Endogenous Control Array to examine housekeeping gene expression across 32 tissues, identifying genes with consistent expression levels suitable for normalization.
NK-cell therapy for Hematologic Malignancies. Prof. Akihiro Shimosaka
This document summarizes Akihiro Shimosaka's credentials and research on expanding natural killer (NK) cells for cancer therapy. It provides details on various methods for expanding NK cells from sources like peripheral blood mononuclear cells (PBMCs) and umbilical cord blood. The methods are compared in terms of fold expansion, cytotoxicity of expanded NK cells, and expression of activating receptors. Clinical cases demonstrate that NK cell therapy can improve outcomes for cancer patients. The document also discusses using expanded NK cells for antibody-dependent cellular cytotoxicity cancer treatment and their potential in allogeneic stem cell transplantation.
The use of reverse genetics to facilitate the growth of FMDV for the product...
The European Commission for the Control of Foot-and-Mouth Disease (EuFMD), one of FAO’s oldest Commissions, came into being on the 12th June 1954, with the pledge of the sixth founding member state to the principles of a coordinated and common action against Foot-and-mouth Disease.
To integrate promoter-GW-lux fragment onto chromosome of E.coli
This document describes integrating a promoter-lux fragment onto the chromosome of E. coli to provide more robust results in promoter assays compared to a plasmid. The lux fragment was amplified by PCR and ligated into the destination vector pGRG25. This new plasmid, pGRG25-pDEW201GWlux, was transformed into E. coli. The promoter sequence was then integrated onto the chromosome using Gateway Technology. Promoter activity was tested under different conditions to evaluate strain backgrounds and DNA damage.
Rennert et al. ASH 2019 talk
Aleta Biotherapeutics biologic bridging protein for the treatment of patients relapsing from CAR-CD19 therapies, presented at #ASH19
3768 rennert
1) CAR T cells specific for CD19 have shown remarkable success in treating blood cancers but have had limited effect on solid tumors which lack the CD19 antigen.
2) The IMPACT technology uses fusion proteins containing the extracellular domain of CD19 linked to single-chain antibodies targeting other antigens to bridge CAR19 T cells to solid tumors.
3) Experiments showed CAR19 T cells redirected by a CD19-anti-HER2 fusion protein were cytotoxic against HER2-positive ovarian cancer cells in vitro, demonstrating the IMPACT technology can redirect CAR T cells to new tumor targets through antigen bridging.
ALETA CNS metastasis program 8 July 2020
Slide deck used for video presentation to the OCTS conference. Her2-positive CNS metastases plague refractory breast cancer patients. Here we present our wholly novel approach to cell therapy for the treatment of these patients. #celltherapy #oncology #breastcancer
oncogene
1) NF-kB is constitutively activated in pancreatic cancer cells but not normal pancreatic cells, suggesting it plays a role in pancreatic tumorigenesis.
2) Expressing a mutant IkBa (IkBaM) that inhibits NF-kB suppressed the tumorigenicity of pancreatic cancer cells in a mouse model.
3) Inhibiting NF-kB reduced expression of pro-survival genes like Bcl-xL and Bcl-2, and reduced VEGF and IL-8 expression which are involved in angiogenesis. This suggests NF-kB inhibition can suppress tumorigenesis by reducing pro-survival and angiogenic factors.
Eddie Senior Seminar! 1
The document summarizes research on expressing HIV-1 antigens in plants as a potential vaccine. The researchers found that transient expression in plants is a viable alternative to transgenic plants. They determined that targeting the HIV-1 Gag protein Pr55Gag and its derivative p17/24 to chloroplasts yielded the highest levels of these proteins. Mice vaccinated with p17/24 produced from chloroplasts developed strong CD4+ and CD8+ T cell responses and HIV-specific antibodies, showing it is a promising vaccine candidate.
Real-time PCR method for detection of the transgenic rice event TT51-1
This document summarizes a research article that developed and validated real-time PCR methods for detecting the transgenic rice event TT51-1. The researchers established both qualitative and quantitative PCR assays to distinguish TT51-1 from other rice varieties. Validation experiments demonstrated the methods could accurately detect down to 10 template copies of the TT51-1 gene. The authors hope this validated detection method can help regulate and control TT51-1 rice.
Detection and Surveillance of Antibiotic Resistance Genes From Food and Ferti...
One potential way to acquire antibiotic resistance genes is through the food supply chain. Both livestock and feed may
acquire antibiotic resistant bacteria via different mechanisms. Foodstuffs can be exposed to antibiotic resistant bacteria
through fertilizer originating from waste-water treatment plants. This, in addition to increasing administration of antibiotics
to livestock, can lead to food being a potential source of antibiotic resistance genes. This may lead to horizontal gene
transfer to pathogenic enteropathogens and further to drug resistance in humans. Therefore, the surveillance and prevention
of antibiotic resistance genes in food is important.
To effectively combat the spread of difficult-to-treat bacterial infections, rapid surveillance methods to detect antibiotic
resistance genes are required; in order to monitor both bacterial isolates and metagenomic samples.
Since the gut is known to act as a reservoir for antibiotic resistance genes, a small-scale research study was performed on
5 stool samples isolated from healthy human adults using an antibiotic resistance gene identification PCR array. In addition,
the diversity of antibiotic resistance genes in municipal biosolids was determined using an Antibiotic Resistance Genes
Microbial DNA qPCR Array with DNA extracted from belt-filter, press-cake sewage samples.
22 antibiotic resistance genes were identified from different resistance classifications. Further studies were performed in
beef, chicken, vegetable and pork samples. In conclusion, PCR arrays can be effective tools for detection of antibiotic
resistance genes from food samples and potential fertilizer sources.
Comparison of Culture Media for In Vitro T Cell Expansion & Function
Presented by Astarte Biologics (astartebio.com) at the Society for Immunotherapy of Cancer (SITC) 33rd Annual Meeting. This research explores a series of studies comparing the performance of several culture media.
AACR poster VP_2014
This document describes research into developing prostate cancer immunotherapy using chimeric antigen receptor (CAR) T cells. The researchers generated two CAR constructs - CAR-PSMA containing an antibody targeting prostate-specific membrane antigen (PSMA), and CAR-NKG2D containing the human NKG2D receptor. They transduced human T cells with these constructs, which resulted in surface expression of the tumor-targeting receptors. In vitro experiments demonstrated the CAR T cells specifically bound and killed prostate cancer cells expressing the target antigens, indicating the potential for an effective CAR T cell immunotherapy for prostate cancer.
What's hot (18)
Agarikon.1 and Agarikon Plus Affect Cell Cycle and Induce Apoptosis in Human ...
Agarikon.1 and Agarikon Plus Affect Cell Cycle and Induce Apoptosis in Human ...
NK-cell therapy for Hematologic Malignancies. Prof. Akihiro Shimosaka
NK-cell therapy for Hematologic Malignancies. Prof. Akihiro Shimosaka
The use of reverse genetics to facilitate the growth of FMDV for the product...
The use of reverse genetics to facilitate the growth of FMDV for the product...
To integrate promoter-GW-lux fragment onto chromosome of E.coli
To integrate promoter-GW-lux fragment onto chromosome of E.coli
Real-time PCR method for detection of the transgenic rice event TT51-1
Real-time PCR method for detection of the transgenic rice event TT51-1
Detection and Surveillance of Antibiotic Resistance Genes From Food and Ferti...
Detection and Surveillance of Antibiotic Resistance Genes From Food and Ferti...
Comparison of Culture Media for In Vitro T Cell Expansion & Function
Comparison of Culture Media for In Vitro T Cell Expansion & Function
Viewers also liked
journal.ppat.1005708 (1)
This research article examines how activation of natural killer (NK) cells impacts susceptibility to Listeria monocytogenes (Lm) infection. The study finds that depletion of NK cells significantly reduces bacterial burdens and increases survival in infected mice, indicating NK cells increase host susceptibility to Lm infection. This effect is independent of early interferon-gamma (IFNγ) production by NK cells, as depletion of NK cells still protected IFNγ receptor-deficient mice. The article further investigates how a bacterial virulence protein, p60, drives NK cells to switch from producing IFNγ to producing the regulatory cytokine IL-10, which suppresses myeloid cell responses and increases bacterial burdens.
Enhanced NK cell adoptive antitumor effects against breast cancer in vitro
This is the research paper which i have been choosen for presentation "Enhance NK cell adoptive antitumor effects against breast cancer in vitro via blockade of the Transforming Growth Factor-Beta".
T cell and NK-cell neoplasms
This document discusses T-cell and NK-cell neoplasms. It begins by describing NK cells and their morphology as large granular lymphocytes that lack T-cell receptors and CD3 expression but express CD56 and CD16. The document then covers the various types of T-cell and NK-cell neoplasms including their morphology, immunophenotype, and genetics. Key neoplasms discussed are T-cell lymphoblastic leukemia/lymphoma, T-cell prolymphocytic leukemia, T-cell large granular lymphocytic leukemia, and extranodal NK/T-cell lymphoma among others. The document provides details on the diagnostic criteria for each type of neoplasm.
CAR-T (Cell Therapy) Nomenclature Review & Brand Equity Study. April 15, 2015
CAR-T (Cell Therapy) Nomenclature Review & Brand Equity Study. April 15, 2015.
Brand Acumen. The Global Leader in Pharmaceutical Name Development and Submission Strategy.
The immune checkpoint landscape in 2015: combination therapy
A comprehensive workshop presentation on approved and developing therapeutics in immuno-oncology, presented at ICI Boston 2015
CAR-T cell therapy in China
This document summarizes the use of CAR-T therapy at the Department of Hematology at Sheng Jing Hospital of China Medical University. It discusses treating lymphoma with CAR-T cells targeting CD19 using a retroviral vector. Patients received chemotherapy with fludarabine and cyclophosphamide before CAR-T cell infusion. Seven patients were treated, including two who received two rounds of therapy. Responses included partial and complete remission in some patients. Cytokine release syndrome occurred in over half the patients and was treated with TNF alpha antagonists.
CAR-T cells in Leukemia. Prof. Keiya Ozawa
This document summarizes Keiya Ozawa's research on CD19-targeted CAR T-cell therapy for B-cell lymphoma. It describes the design of CARs to target the CD19 antigen on B-cells, experimental data showing CD19-CAR T-cells effectively killed CD19-positive lymphoma cells in vitro and in mouse models, and outlines Ozawa's ongoing clinical trial testing CD19-CAR T-cell therapy in patients with relapsed/refractory B-cell lymphoma. The clinical trial uses lymphodepletion prior to infusing autologous T-cells engineered to express an anti-CD19 CAR to determine the maximum tolerated dose.
Viewers also liked (7)
Enhanced NK cell adoptive antitumor effects against breast cancer in vitro
Enhanced NK cell adoptive antitumor effects against breast cancer in vitro
CAR-T (Cell Therapy) Nomenclature Review & Brand Equity Study. April 15, 2015
CAR-T (Cell Therapy) Nomenclature Review & Brand Equity Study. April 15, 2015
The immune checkpoint landscape in 2015: combination therapy
The immune checkpoint landscape in 2015: combination therapy
Similar to Poster C Linical Nk Generation Ash 2009 Js
Cord Blood Natural Killer Cells for Immunotherapy
Cord blood is a potential source of natural killer (NK) cells for cancer immunotherapy. NK cells can be expanded from cord blood units to sufficient clinical scales for treatment. Expanded cord blood NK cells have been shown to be as cytotoxic against tumors as peripheral blood NK cells. They have demonstrated activity against multiple myeloma and chronic lymphocytic leukemia in preclinical studies. A phase I/II clinical trial is exploring the use of ex vivo expanded cord blood NK cells combined with chemotherapy and autologous stem cell transplant for multiple myeloma. Additional strategies are being investigated to enhance the anti-tumor activity of cord blood NK cells, such as using lenalidomide or engineering the cells to improve homing to the bone marrow micro
Cellular Immune Therapy with Allogeneic Stem Cell Transplantation
This document discusses cellular immune therapy approaches using hematopoietic stem cell transplantation. It notes that high-dose chemotherapy alone does not fully eradicate malignancies and relapse remains a major treatment failure. The graft-versus-malignancy effect provided by allogeneic transplantation is responsible for residual disease eradication but is associated with graft-versus-host disease. The document reviews approaches to enhance the graft-versus-malignancy effect through donor lymphocyte infusions, antigen-specific cytotoxic T-cells, and chimeric antigen receptor T-cells while preventing graft-versus-host disease using regulatory T-cells or genetically modified T-cells with suicide switches. Ongoing clinical trials
From syngenic to_humanised_models_mirjolet_oncodesign
Discovery of novel immunotherapy represents a main and intense focus of reseach in oncology. Proof of concept studies in animal represent a challenge and require a well characterized and appropriate animal models with most of the time customized approaches. Some recent developments and data generated for immune checkpoint modulators, adoptive cell transfer therapy, vaccines and bispecific T cell engagers will be presented.
Cart cell in rheumatology ppt.pdf
CAR T cells show promise for treating rheumatologic diseases. They involve genetically modifying a patient's T cells to express a chimeric antigen receptor (CAR) that targets specific proteins on autoreactive B cells. First-generation CAR T cells contain an antibody-derived antigen binding domain, while later generations add costimulatory domains for improved activation and persistence. CAR T cells have depleted B cells and reduced autoantibodies in mouse models of lupus and human case studies of severe refractory SLE. They have also shown effectiveness against antisynthetase syndrome by reversing manifestations after other treatments failed. Further studies are still needed regarding their long-term safety, durability of remission, and cost effectiveness before they can be widely
Stemline® XF MSC Medium has High Yield and Functionality in the 3 L Mobius® S...
Learn about process parameters and growth results of bone marrow-derived hMSCs cultured in Stemline® XF MSC Medium in a 3 L stirred tank bioreactor-microcarrier platform.
Measure Target Cancer Cell Death in Co-Cultures
Learn about a powerful new cell-based assay for specifically measuring target cancer cell death in co-cultures. Very useful in the development of immunotherapy drugs.
Cd34_cord_blood_paper[1]
This document describes a new flow cytometric method called VAC34 for measuring absolute concentrations and viability of CD34+ cells in cord blood and cord blood products. Some key points:
- The VAC34 method uses fluorescent beads, antibodies against CD34 and CD45, and the nucleic acid dye YO-PRO-1 to provide absolute CD34+ cell counts and assess cell viability in a single, wash-free procedure.
- Comparison to the ProCOUNT assay in 57 samples showed excellent correlation (r=0.98) between the two methods.
- Precision studies yielded coefficients of variation of 6.4% and 8.9% at different CD34+ cell concentrations, indicating good precision.
Actualización sobre la producción de reactivos y pruebas comerciales disponib...
Actualización sobre la producción de reactivos y pruebas comerciales disponib...Organización Panamericana de la Salud (OPS/OMS en Argentina)
Jornadas de arbovirosis: dengue, chikungunya y Zika. Preparación y respuesta de los países del Cono Sur. Buenos Aires, Argentina, 4-5 noviembre 2015.Targeted Single Cell Sequencing for Accurate Mutation Detection in Heterogene...
Targeted Single Cell Sequencing for Accurate Mutation Detection in Heterogeneous Cellular Populations
Generation of MRP2 Efflux Transporter Knock-Out in HepaRG Cell Line
The document describes experiments characterizing a HepaRG cell line with MRP2 (an efflux transporter) knocked out using zinc finger nuclease technology. Western blot and immunostaining showed loss of MRP2 protein expression in knockout cells. Assays found control cells accumulated the fluorescent MRP2 substrate CDCF in bile canaliculi, while knockout cells did not, demonstrating functional loss of MRP2. The MRP2 knockout cell line allows investigation of MRP2-associated drug toxicity without its protective efflux.
Paul O'Gorman placement presentation
The Paul O'Gorman Leukemia Research Centre is focused on understanding hematopoietic stem cells and their role in leukemia development. The center aims to identify new therapeutic targets for leukemia by exploring CML stem/progenitor cells. The document outlines a 10-week project to produce an RNA bank from CML cell lines and patient samples and characterize the samples through colony formation assays, PCR to determine BCR-ABL breakpoints, flow cytometry for CD34/CD38 expression, and FISH to visualize the fusion oncogene. Preliminary results are shown for several patient samples that were analyzed.
Gene Expression Analysis by Real Time PCR
Nowadays, Real Time-PCR analysis has become the method of choice for gene expression studies and validating transcriptomic data.
Comparación métodos detección carbapenemasas.pptx
This document describes a study that evaluated a novel multiplex real-time PCR assay for rapid detection of Mycobacterium tuberculosis complex (MTBC) directly from various clinical samples. The assay targets two conserved genes, whiB3 and pstS1. It was tested on 94 clinical samples and showed 88% sensitivity and 95% specificity compared to culture. The assay demonstrated excellent detection of MTBC from samples with low bacterial loads and could be a valuable tool for rapid diagnosis of extrapulmonary TB.
Improved Platforms for CHO Cell Line Development
Review of the integration of CHOZN and UCOE cell line development platforms, and the performance of the combined systems.
Joseph Gligorov : Lipegfilgrastim : A new long-ac,ng recombinant human G-CSF
Joseph Gligorov : Lipegfilgrastim : A new long-ac,ng recombinant human G-CSFbreastcancerupdatecongress
This document summarizes clinical trial results of a new long-acting recombinant human G-CSF called lipegfilgrastim. Phase I studies in healthy volunteers found that lipegfilgrastim had a longer half-life and higher area under the curve compared to pegfilgrastim at equivalent doses, resulting in higher absolute neutrophil counts and CD34+ cell counts over time. Adverse events were mostly mild to moderate and consistent between the two drugs. Phase II studies are ongoing to evaluate the efficacy and safety of different doses of lipegfilgrastim compared to pegfilgrastim in breast cancer patients undergoing chemotherapy.Dr. David Mooney - Simposio Internacional 'Terapias oncológicas avanzadas'
Los días 15 y 16 de octubre de 2014, la Fundación Ramón Areces y la Real Academia Nacional de Farmacia, en colaboración con la Fundación de la Innovación Bankinter, reunieron en Madrid a algunos de los mayores expertos mundiales en nuevas terapias contra el cáncer. El Simposio Internacional, coordinado por la profesora y académica María José Alonso, analizó el momento actual de la lucha contra esta enfermedad. También fue un punto de encuentro para científicos de los más innovadores institutos de investigación en oncología, quienes debatieron sobre tres grandes temas: la Medicina Personalizada contra el cáncer, los nanomedicamentos en la terapia del cáncer y las terapias basadas en la inmunomodulación.
Accelerate Delivery of High Producing Cell Lines
Watch the interactive recording here: https://bit.ly/30FTDG0
The quest for a viable upstream process relies on generation of a cell line expressing the protein of interest. Unfortunately, the search for the best-producing clone is often compared with looking for a needle in a haystack. Making this more challenging is the pressure to get it right the first time, quickly and while mitigating risk and costs.
Although a lot of efforts are made on the clonal selection, there is often few to none optimization done on the expression cassette, including promoter and enhancer selection, or signal peptide. The statistical approach on how many clones should be screened to get to a good producer is often overlooked as well.
We combined a new generation of promoters and enhancers to improve strategies on pool and mini pool screening with both CHO-K1 and our own CHOZN® GS which helped deliver high-producing clones in an accelerated timeline. In addition, we are able to begin process development in parallel with cell line development, further reducing timelines.
In this webinar, you will learn:
* How the strategy approach can help reducing the overall timeline of cell line generation
* How we have expanded our platform by designing a completely new vector/cell/process template
* How we have worked on promoters, enhancers, pool/mini-pool approach as well as on timelines from DNA to clone
Accelerate Delivery of High Producing Cell Lines
Merck KGaA provides cell line development and manufacturing services to support customers' biologics drug development programs, with over 30 years of experience developing over 250 cell lines. They have extensive expertise in cell line development, process development, analytical testing, and GMP manufacturing across multiple sites globally. The document highlights some of Merck KGaA's capabilities and case studies, including their approaches to optimizing cell line development techniques and processes like mini-pool screening and signal peptide selection to generate high producing cell lines.
Current and Novel Immuno-Oncology Drug Evaluation Methods via Humanized Mouse...
Dr. Bin Xie discusses the current immuno-oncology drug development landscape, different humanized mouse models available for drug testing, and the investigation of potential mechanisms via imaging mass cytometry.
Since the first immune checkpoint blocker ipilimumab was approved by the US FDA in 2011, more drug companies have sought to develop their own immune therapy drugs. Humanized peripheral blood mononuclear cell (PBMC) reconstitution in immune deficient mice is becoming a valuable model for evaluating therapeutic antibodies, especially bispecific antibodies (BsAbs), which can mediate immune cells as well as target a tumor antigen.
However, this model has several drawbacks, including a limited dosing window due to graft-versus-host-disease and insufficient natural immune cell infiltration. This has hindered wide application of the model in the development of multiple immune checkpoint inhibitors or immune agonists.
To overcome these issues, LIDE has developed a unique human PBMC/cancer cell co-transfer model which can generate three-dimensional huPBMC-infiltrated tumor tissue for immunotherapy. This model has successfully been used to evaluate the biological function of several signaling proteins and biomarkers in multiple cancers, such as melanoma, breast cancer, and lung cancer.
In this webinar, Dr. Bin Xie discusses the current immuno-oncology drug development landscape, different humanized models available for drug testing, evaluates real-world case studies, and describes the investigation of potential mechanisms by imaging mass cytometry.
Key Topics Include:
- Introduction to immuno-oncology drug development and the importance of using humanized mouse models to address scientific questions
- Evaluation of current IO platforms and new methods from LIDE, including analysis of several case studies
- Understanding the spatiotemporal interaction between tissue-infiltrating immune cells and cancer cells via imaging mass cytometry
NiH_Presentation
1) The document summarizes research into genetic interactors of the BRCA2 tumor suppressor gene, which is linked to hereditary breast cancer. A retroviral screening identified BRE as a genetic interactor that rescues lethality in BRCA2-deficient cells.
2) Further experiments showed that BRE overexpression in BRCA2-deficient cells leads to increased levels of the Cdc25A cell cycle regulator after DNA damage, preventing cell cycle arrest.
3) BRE was found to interact with the transcription factor ATF3 and induce transcription of Cdc25A. Reporter assays aim to identify the role of ATF3 binding sites on the Cdc25A promoter in regulating its transcription
Similar to Poster C Linical Nk Generation Ash 2009 Js (20)
Cellular Immune Therapy with Allogeneic Stem Cell Transplantation
Cellular Immune Therapy with Allogeneic Stem Cell Transplantation
From syngenic to_humanised_models_mirjolet_oncodesign
From syngenic to_humanised_models_mirjolet_oncodesign
Stemline® XF MSC Medium has High Yield and Functionality in the 3 L Mobius® S...
Stemline® XF MSC Medium has High Yield and Functionality in the 3 L Mobius® S...
Actualización sobre la producción de reactivos y pruebas comerciales disponib...
Actualización sobre la producción de reactivos y pruebas comerciales disponib...
Targeted Single Cell Sequencing for Accurate Mutation Detection in Heterogene...
Targeted Single Cell Sequencing for Accurate Mutation Detection in Heterogene...
Generation of MRP2 Efflux Transporter Knock-Out in HepaRG Cell Line
Generation of MRP2 Efflux Transporter Knock-Out in HepaRG Cell Line
Joseph Gligorov : Lipegfilgrastim : A new long-ac,ng recombinant human G-CSF
Joseph Gligorov : Lipegfilgrastim : A new long-ac,ng recombinant human G-CSF
Dr. David Mooney - Simposio Internacional 'Terapias oncológicas avanzadas'
Dr. David Mooney - Simposio Internacional 'Terapias oncológicas avanzadas'
Current and Novel Immuno-Oncology Drug Evaluation Methods via Humanized Mouse...
Current and Novel Immuno-Oncology Drug Evaluation Methods via Humanized Mouse...
Recently uploaded
JavaLand 2024: Application Development Green Masterplan
My presentation slides I used at JavaLand 2024
June Patch Tuesday
Ivanti’s Patch Tuesday breakdown goes beyond patching your applications and brings you the intelligence and guidance needed to prioritize where to focus your attention first. Catch early analysis on our Ivanti blog, then join industry expert Chris Goettl for the Patch Tuesday Webinar Event. There we’ll do a deep dive into each of the bulletins and give guidance on the risks associated with the newly-identified vulnerabilities.
Driving Business Innovation: Latest Generative AI Advancements & Success Story
Are you ready to revolutionize how you handle data? Join us for a webinar where we’ll bring you up to speed with the latest advancements in Generative AI technology and discover how leveraging FME with tools from giants like Google Gemini, Amazon, and Microsoft OpenAI can supercharge your workflow efficiency.
During the hour, we’ll take you through:
Guest Speaker Segment with Hannah Barrington: Dive into the world of dynamic real estate marketing with Hannah, the Marketing Manager at Workspace Group. Hear firsthand how their team generates engaging descriptions for thousands of office units by integrating diverse data sources—from PDF floorplans to web pages—using FME transformers, like OpenAIVisionConnector and AnthropicVisionConnector. This use case will show you how GenAI can streamline content creation for marketing across the board.
Ollama Use Case: Learn how Scenario Specialist Dmitri Bagh has utilized Ollama within FME to input data, create custom models, and enhance security protocols. This segment will include demos to illustrate the full capabilities of FME in AI-driven processes.
Custom AI Models: Discover how to leverage FME to build personalized AI models using your data. Whether it’s populating a model with local data for added security or integrating public AI tools, find out how FME facilitates a versatile and secure approach to AI.
We’ll wrap up with a live Q&A session where you can engage with our experts on your specific use cases, and learn more about optimizing your data workflows with AI.
This webinar is ideal for professionals seeking to harness the power of AI within their data management systems while ensuring high levels of customization and security. Whether you're a novice or an expert, gain actionable insights and strategies to elevate your data processes. Join us to see how FME and AI can revolutionize how you work with data!
Skybuffer AI: Advanced Conversational and Generative AI Solution on SAP Busin...
Skybuffer AI, built on the robust SAP Business Technology Platform (SAP BTP), is the latest and most advanced version of our AI development, reaffirming our commitment to delivering top-tier AI solutions. Skybuffer AI harnesses all the innovative capabilities of the SAP BTP in the AI domain, from Conversational AI to cutting-edge Generative AI and Retrieval-Augmented Generation (RAG). It also helps SAP customers safeguard their investments into SAP Conversational AI and ensure a seamless, one-click transition to SAP Business AI.
With Skybuffer AI, various AI models can be integrated into a single communication channel such as Microsoft Teams. This integration empowers business users with insights drawn from SAP backend systems, enterprise documents, and the expansive knowledge of Generative AI. And the best part of it is that it is all managed through our intuitive no-code Action Server interface, requiring no extensive coding knowledge and making the advanced AI accessible to more users.
Azure API Management to expose backend services securely
How to use Azure API Management to expose backend service securely
HCL Notes and Domino License Cost Reduction in the World of DLAU
Webinar Recording: https://www.panagenda.com/webinars/hcl-notes-and-domino-license-cost-reduction-in-the-world-of-dlau/
The introduction of DLAU and the CCB & CCX licensing model caused quite a stir in the HCL community. As a Notes and Domino customer, you may have faced challenges with unexpected user counts and license costs. You probably have questions on how this new licensing approach works and how to benefit from it. Most importantly, you likely have budget constraints and want to save money where possible. Don’t worry, we can help with all of this!
We’ll show you how to fix common misconfigurations that cause higher-than-expected user counts, and how to identify accounts which you can deactivate to save money. There are also frequent patterns that can cause unnecessary cost, like using a person document instead of a mail-in for shared mailboxes. We’ll provide examples and solutions for those as well. And naturally we’ll explain the new licensing model.
Join HCL Ambassador Marc Thomas in this webinar with a special guest appearance from Franz Walder. It will give you the tools and know-how to stay on top of what is going on with Domino licensing. You will be able lower your cost through an optimized configuration and keep it low going forward.
These topics will be covered
- Reducing license cost by finding and fixing misconfigurations and superfluous accounts
- How do CCB and CCX licenses really work?
- Understanding the DLAU tool and how to best utilize it
- Tips for common problem areas, like team mailboxes, functional/test users, etc
- Practical examples and best practices to implement right away
Introduction of Cybersecurity with OSS at Code Europe 2024
I develop the Ruby programming language, RubyGems, and Bundler, which are package managers for Ruby. Today, I will introduce how to enhance the security of your application using open-source software (OSS) examples from Ruby and RubyGems.
The first topic is CVE (Common Vulnerabilities and Exposures). I have published CVEs many times. But what exactly is a CVE? I'll provide a basic understanding of CVEs and explain how to detect and handle vulnerabilities in OSS.
Next, let's discuss package managers. Package managers play a critical role in the OSS ecosystem. I'll explain how to manage library dependencies in your application.
I'll share insights into how the Ruby and RubyGems core team works to keep our ecosystem safe. By the end of this talk, you'll have a better understanding of how to safeguard your code.
5th LF Energy Power Grid Model Meet-up Slides
5th Power Grid Model Meet-up
It is with great pleasure that we extend to you an invitation to the 5th Power Grid Model Meet-up, scheduled for 6th June 2024. This event will adopt a hybrid format, allowing participants to join us either through an online Mircosoft Teams session or in person at TU/e located at Den Dolech 2, Eindhoven, Netherlands. The meet-up will be hosted by Eindhoven University of Technology (TU/e), a research university specializing in engineering science & technology.
Power Grid Model
The global energy transition is placing new and unprecedented demands on Distribution System Operators (DSOs). Alongside upgrades to grid capacity, processes such as digitization, capacity optimization, and congestion management are becoming vital for delivering reliable services.
Power Grid Model is an open source project from Linux Foundation Energy and provides a calculation engine that is increasingly essential for DSOs. It offers a standards-based foundation enabling real-time power systems analysis, simulations of electrical power grids, and sophisticated what-if analysis. In addition, it enables in-depth studies and analysis of the electrical power grid’s behavior and performance. This comprehensive model incorporates essential factors such as power generation capacity, electrical losses, voltage levels, power flows, and system stability.
Power Grid Model is currently being applied in a wide variety of use cases, including grid planning, expansion, reliability, and congestion studies. It can also help in analyzing the impact of renewable energy integration, assessing the effects of disturbances or faults, and developing strategies for grid control and optimization.
What to expect
For the upcoming meetup we are organizing, we have an exciting lineup of activities planned:
-Insightful presentations covering two practical applications of the Power Grid Model.
-An update on the latest advancements in Power Grid -Model technology during the first and second quarters of 2024.
-An interactive brainstorming session to discuss and propose new feature requests.
-An opportunity to connect with fellow Power Grid Model enthusiasts and users.
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Sidekick Solutions uses Bonterra Impact Management (fka Social Solutions Apricot) and automation solutions to integrate data for business workflows.
We believe integration and automation are essential to user experience and the promise of efficient work through technology. Automation is the critical ingredient to realizing that full vision. We develop integration products and services for Bonterra Case Management software to support the deployment of automations for a variety of use cases.
This video focuses on integration of Salesforce with Bonterra Impact Management.
Interested in deploying an integration with Salesforce for Bonterra Impact Management? Contact us at sales@sidekicksolutionsllc.com to discuss next steps.
FREE A4 Cyber Security Awareness Posters-Social Engineering part 3
Free A4 downloadable and printable Cyber Security, Social Engineering Safety and security Training Posters . Promote security awareness in the home or workplace. Lock them Out From training providers datahops.com
AWS Cloud Cost Optimization Presentation.pptx
This presentation provides valuable insights into effective cost-saving techniques on AWS. Learn how to optimize your AWS resources by rightsizing, increasing elasticity, picking the right storage class, and choosing the best pricing model. Additionally, discover essential governance mechanisms to ensure continuous cost efficiency. Whether you are new to AWS or an experienced user, this presentation provides clear and practical tips to help you reduce your cloud costs and get the most out of your budget.
Programming Foundation Models with DSPy - Meetup Slides
Prompting language models is hard, while programming language models is easy. In this talk, I will discuss the state-of-the-art framework DSPy for programming foundation models with its powerful optimizers and runtime constraint system.
SAP S/4 HANA sourcing and procurement to Public cloud
SAP S4 HANA to Public cloud data object differences
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
Simplify your search for a reliable Python development partner! This list presents the top 10 trusted US providers offering comprehensive Python development services, ensuring your project's success from conception to completion.
“Temporal Event Neural Networks: A More Efficient Alternative to the Transfor...
“Temporal Event Neural Networks: A More Efficient Alternative to the Transfor...Edge AI and Vision Alliance
For the full video of this presentation, please visit: https://www.edge-ai-vision.com/2024/06/temporal-event-neural-networks-a-more-efficient-alternative-to-the-transformer-a-presentation-from-brainchip/
Chris Jones, Director of Product Management at BrainChip , presents the “Temporal Event Neural Networks: A More Efficient Alternative to the Transformer” tutorial at the May 2024 Embedded Vision Summit.
The expansion of AI services necessitates enhanced computational capabilities on edge devices. Temporal Event Neural Networks (TENNs), developed by BrainChip, represent a novel and highly efficient state-space network. TENNs demonstrate exceptional proficiency in handling multi-dimensional streaming data, facilitating advancements in object detection, action recognition, speech enhancement and language model/sequence generation. Through the utilization of polynomial-based continuous convolutions, TENNs streamline models, expedite training processes and significantly diminish memory requirements, achieving notable reductions of up to 50x in parameters and 5,000x in energy consumption compared to prevailing methodologies like transformers.
Integration with BrainChip’s Akida neuromorphic hardware IP further enhances TENNs’ capabilities, enabling the realization of highly capable, portable and passively cooled edge devices. This presentation delves into the technical innovations underlying TENNs, presents real-world benchmarks, and elucidates how this cutting-edge approach is positioned to revolutionize edge AI across diverse applications.Best 20 SEO Techniques To Improve Website Visibility In SERP
Boost your website's visibility with proven SEO techniques! Our latest blog dives into essential strategies to enhance your online presence, increase traffic, and rank higher on search engines. From keyword optimization to quality content creation, learn how to make your site stand out in the crowded digital landscape. Discover actionable tips and expert insights to elevate your SEO game.
Taking AI to the Next Level in Manufacturing.pdf
Read Taking AI to the Next Level in Manufacturing to gain insights on AI adoption in the manufacturing industry, such as:
1. How quickly AI is being implemented in manufacturing.
2. Which barriers stand in the way of AI adoption.
3. How data quality and governance form the backbone of AI.
4. Organizational processes and structures that may inhibit effective AI adoption.
6. Ideas and approaches to help build your organization's AI strategy.
Digital Marketing Trends in 2024 | Guide for Staying Ahead
https://www.wask.co/ebooks/digital-marketing-trends-in-2024
Feeling lost in the digital marketing whirlwind of 2024? Technology is changing, consumer habits are evolving, and staying ahead of the curve feels like a never-ending pursuit. This e-book is your compass. Dive into actionable insights to handle the complexities of modern marketing. From hyper-personalization to the power of user-generated content, learn how to build long-term relationships with your audience and unlock the secrets to success in the ever-shifting digital landscape.
Recently uploaded (20)
JavaLand 2024: Application Development Green Masterplan
JavaLand 2024: Application Development Green Masterplan
Driving Business Innovation: Latest Generative AI Advancements & Success Story
Driving Business Innovation: Latest Generative AI Advancements & Success Story
Skybuffer AI: Advanced Conversational and Generative AI Solution on SAP Busin...
Skybuffer AI: Advanced Conversational and Generative AI Solution on SAP Busin...
Azure API Management to expose backend services securely
Azure API Management to expose backend services securely
HCL Notes and Domino License Cost Reduction in the World of DLAU
HCL Notes and Domino License Cost Reduction in the World of DLAU
Introduction of Cybersecurity with OSS at Code Europe 2024
Introduction of Cybersecurity with OSS at Code Europe 2024
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
FREE A4 Cyber Security Awareness Posters-Social Engineering part 3
FREE A4 Cyber Security Awareness Posters-Social Engineering part 3
Programming Foundation Models with DSPy - Meetup Slides
Programming Foundation Models with DSPy - Meetup Slides
SAP S/4 HANA sourcing and procurement to Public cloud
SAP S/4 HANA sourcing and procurement to Public cloud
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
Nordic Marketo Engage User Group_June 13_ 2024.pptx
Nordic Marketo Engage User Group_June 13_ 2024.pptx
“Temporal Event Neural Networks: A More Efficient Alternative to the Transfor...
“Temporal Event Neural Networks: A More Efficient Alternative to the Transfor...
Best 20 SEO Techniques To Improve Website Visibility In SERP
Best 20 SEO Techniques To Improve Website Visibility In SERP
WeTestAthens: Postman's AI & Automation Techniques
WeTestAthens: Postman's AI & Automation Techniques
Digital Marketing Trends in 2024 | Guide for Staying Ahead
Digital Marketing Trends in 2024 | Guide for Staying Ahead