PARP-1 inhibitors have shown promise in oncology by potentiating the effects of DNA damaging chemotherapy agents. Cephalon identified a pyrrolocarbazole hit that inhibited PARP-1 but had poor properties. Through structure-based design and SAR studies, they developed CEP-8983, a potent PARP inhibitor. A prodrug, CEP-9722, was synthesized to improve solubility and pharmacokinetics. In preclinical studies, CEP-8983 and its active metabolite CEP-9397 potentiated the effects of temozolomide in tumor cell lines. CEP-9722 advanced to Phase 1 and 2 clinical trials for evaluation as monotherapy and
Lung cancer is a major cause of cancer deaths with approximately 80% of cases accounting to nonsmall cell lung cancer (NSCLC) . In NSCLC target therapy, epidermal growth factor receptor (EGFR) is a promising candidate.
Tried to summarise all landmark trials in carcinoma breast in radiation oncology,medical oncology as well in surgical oncology.
References taken from Devita Book,Breast Disease book from Springer,journals like NEJM,JAMA,LANCET,ANNL ONCOLOGY etc,internet,Perez book,Practical Clinical Oncology by Hanna etc textbooks.
Thanks.
Chair and Presenter, Prof Eric Van Cutsem, MD, PhD, and Scott Kopetz, MD, PhD, prepared useful Practice Aids pertaining to colorectal cancer for this CME/MOC/NCPD activity titled “Putting a Personalized Colorectal Cancer Treatment Algorithm Into Practice: Navigating Practicalities in the Era of Molecularly Defined Care.” For the full presentation, downloadable Practice Aids, and complete CME/MOC/NCPD information, and to apply for credit, please visit us at https://bit.ly/3aSSAtm. CME/MOC/NCPD credit will be available until November 13, 2022.
Audio and slides for this presentation are available on YouTube: http://youtu.be/ozNSEND5PbE
Erica Mayer, MD, MPH, of the Susan F. Smith Center for Women's Cancers at Dana-Farber Cancer Institute, discusses triple-negative breast cancer and what makes it different from other forms of breast cancer. Mayer also talks about treatment options for triple-negative breast cancer and what you need to know about clinical trials for the disease.
Overview about evolution of the term Oligometastases,the paradigm and various states of oligometastases,treat options ,clinical trials and relevance in current clinical practice
Introduction to Targeted Therapies in OncologyMohamed Abdulla
Describes the molecular background which represents the core for developing targeted therapies against specific biological events in malignant cellular clones.
Lung cancer is a major cause of cancer deaths with approximately 80% of cases accounting to nonsmall cell lung cancer (NSCLC) . In NSCLC target therapy, epidermal growth factor receptor (EGFR) is a promising candidate.
Tried to summarise all landmark trials in carcinoma breast in radiation oncology,medical oncology as well in surgical oncology.
References taken from Devita Book,Breast Disease book from Springer,journals like NEJM,JAMA,LANCET,ANNL ONCOLOGY etc,internet,Perez book,Practical Clinical Oncology by Hanna etc textbooks.
Thanks.
Chair and Presenter, Prof Eric Van Cutsem, MD, PhD, and Scott Kopetz, MD, PhD, prepared useful Practice Aids pertaining to colorectal cancer for this CME/MOC/NCPD activity titled “Putting a Personalized Colorectal Cancer Treatment Algorithm Into Practice: Navigating Practicalities in the Era of Molecularly Defined Care.” For the full presentation, downloadable Practice Aids, and complete CME/MOC/NCPD information, and to apply for credit, please visit us at https://bit.ly/3aSSAtm. CME/MOC/NCPD credit will be available until November 13, 2022.
Audio and slides for this presentation are available on YouTube: http://youtu.be/ozNSEND5PbE
Erica Mayer, MD, MPH, of the Susan F. Smith Center for Women's Cancers at Dana-Farber Cancer Institute, discusses triple-negative breast cancer and what makes it different from other forms of breast cancer. Mayer also talks about treatment options for triple-negative breast cancer and what you need to know about clinical trials for the disease.
Overview about evolution of the term Oligometastases,the paradigm and various states of oligometastases,treat options ,clinical trials and relevance in current clinical practice
Introduction to Targeted Therapies in OncologyMohamed Abdulla
Describes the molecular background which represents the core for developing targeted therapies against specific biological events in malignant cellular clones.
Is It Safe? A Cautionary Tale and Practical Guide to Laboratory Safety and C...Gregory J. Wells
Review of several recent tragic lab accidents, some common elements that lead to them, and guidelines and controls for accident prevention. Summary of the OSHA Lab Standard Regulations (29 CFR 1910.1450).
"Brief Introduction of China Food & Drug Administration" by Chang Yongheng, China Centre for Food and Drug International Exchange, China Food & Drug Administration
An Introduction of Healthcare Market in ChinaZiqian WANG
A research on healthcare market in China covering topics including an introduction of Public Hospital System, Chinese Physicians' Work Condition and Salaries, as well as Continued Medical Education system.
Regulatory Highlights and Drug Development in ChinaMedpace
Regulatory Highlights and Drug Development in China was presented at the 5th China Clinical Trials Outsourcing Congress March 4-5, 2013 by Xiaoxiong (Jim) Wei, MD, PhD, Medical Director at Medpace.
What's the latest in breast cancer treatment and research? Erica Mayer, MD, MPH, a medical oncologist in the Susan F. Smith Center for Women's Cancers, shares the latest breast cancer news.
This presentation was originally given on Oct. 16, 2015, at the annual Young Women with Breast Cancer Forum, hosted by the Program for Young Women with Breast Cancer in the Susan F. Smith Center for Women's Cancers at Dana-Farber Cancer Institute, in Boston, Mass.
Learn more: http://www.susanfsmith.org
Low self-esteem affects the way you see yourself, do your job, and relate with the people around you. Learn to overcome it with these quick tips.
More themed slides: https://slideshop.com/Themed-Slides
Skye Residences | Extended Stay Residences Near Toronto Airportmarketingjdass
Experience unparalleled EXTENDED STAY and comfort at Skye Residences located just minutes from Toronto Airport. Discover sophisticated accommodations tailored for discerning travelers.
Website Link :
https://skyeresidences.com/
https://skyeresidences.com/about-us/
https://skyeresidences.com/gallery/
https://skyeresidences.com/rooms/
https://skyeresidences.com/near-by-attractions/
https://skyeresidences.com/commute/
https://skyeresidences.com/contact/
https://skyeresidences.com/queen-suite-with-sofa-bed/
https://skyeresidences.com/queen-suite-with-sofa-bed-and-balcony/
https://skyeresidences.com/queen-suite-with-sofa-bed-accessible/
https://skyeresidences.com/2-bedroom-deluxe-queen-suite-with-sofa-bed/
https://skyeresidences.com/2-bedroom-deluxe-king-queen-suite-with-sofa-bed/
https://skyeresidences.com/2-bedroom-deluxe-queen-suite-with-sofa-bed-accessible/
#Skye Residences Etobicoke, #Skye Residences Near Toronto Airport, #Skye Residences Toronto, #Skye Hotel Toronto, #Skye Hotel Near Toronto Airport, #Hotel Near Toronto Airport, #Near Toronto Airport Accommodation, #Suites Near Toronto Airport, #Etobicoke Suites Near Airport, #Hotel Near Toronto Pearson International Airport, #Toronto Airport Suite Rentals, #Pearson Airport Hotel Suites
RMD24 | Retail media: hoe zet je dit in als je geen AH of Unilever bent? Heid...BBPMedia1
Grote partijen zijn al een tijdje onderweg met retail media. Ondertussen worden in dit domein ook de kansen zichtbaar voor andere spelers in de markt. Maar met die kansen ontstaan ook vragen: Zelf retail media worden of erop adverteren? In welke fase van de funnel past het en hoe integreer je het in een mediaplan? Wat is nu precies het verschil met marketplaces en Programmatic ads? In dit half uur beslechten we de dilemma's en krijg je antwoorden op wanneer het voor jou tijd is om de volgende stap te zetten.
Discover the innovative and creative projects that highlight my journey throu...dylandmeas
Discover the innovative and creative projects that highlight my journey through Full Sail University. Below, you’ll find a collection of my work showcasing my skills and expertise in digital marketing, event planning, and media production.
Putting the SPARK into Virtual Training.pptxCynthia Clay
This 60-minute webinar, sponsored by Adobe, was delivered for the Training Mag Network. It explored the five elements of SPARK: Storytelling, Purpose, Action, Relationships, and Kudos. Knowing how to tell a well-structured story is key to building long-term memory. Stating a clear purpose that doesn't take away from the discovery learning process is critical. Ensuring that people move from theory to practical application is imperative. Creating strong social learning is the key to commitment and engagement. Validating and affirming participants' comments is the way to create a positive learning environment.
3.0 Project 2_ Developing My Brand Identity Kit.pptxtanyjahb
A personal brand exploration presentation summarizes an individual's unique qualities and goals, covering strengths, values, passions, and target audience. It helps individuals understand what makes them stand out, their desired image, and how they aim to achieve it.
Personal Brand Statement:
As an Army veteran dedicated to lifelong learning, I bring a disciplined, strategic mindset to my pursuits. I am constantly expanding my knowledge to innovate and lead effectively. My journey is driven by a commitment to excellence, and to make a meaningful impact in the world.
The world of search engine optimization (SEO) is buzzing with discussions after Google confirmed that around 2,500 leaked internal documents related to its Search feature are indeed authentic. The revelation has sparked significant concerns within the SEO community. The leaked documents were initially reported by SEO experts Rand Fishkin and Mike King, igniting widespread analysis and discourse. For More Info:- https://news.arihantwebtech.com/search-disrupted-googles-leaked-documents-rock-the-seo-world/
What is the TDS Return Filing Due Date for FY 2024-25.pdfseoforlegalpillers
It is crucial for the taxpayers to understand about the TDS Return Filing Due Date, so that they can fulfill your TDS obligations efficiently. Taxpayers can avoid penalties by sticking to the deadlines and by accurate filing of TDS. Timely filing of TDS will make sure about the availability of tax credits. You can also seek the professional guidance of experts like Legal Pillers for timely filing of the TDS Return.
Memorandum Of Association Constitution of Company.pptseri bangash
www.seribangash.com
A Memorandum of Association (MOA) is a legal document that outlines the fundamental principles and objectives upon which a company operates. It serves as the company's charter or constitution and defines the scope of its activities. Here's a detailed note on the MOA:
Contents of Memorandum of Association:
Name Clause: This clause states the name of the company, which should end with words like "Limited" or "Ltd." for a public limited company and "Private Limited" or "Pvt. Ltd." for a private limited company.
https://seribangash.com/article-of-association-is-legal-doc-of-company/
Registered Office Clause: It specifies the location where the company's registered office is situated. This office is where all official communications and notices are sent.
Objective Clause: This clause delineates the main objectives for which the company is formed. It's important to define these objectives clearly, as the company cannot undertake activities beyond those mentioned in this clause.
www.seribangash.com
Liability Clause: It outlines the extent of liability of the company's members. In the case of companies limited by shares, the liability of members is limited to the amount unpaid on their shares. For companies limited by guarantee, members' liability is limited to the amount they undertake to contribute if the company is wound up.
https://seribangash.com/promotors-is-person-conceived-formation-company/
Capital Clause: This clause specifies the authorized capital of the company, i.e., the maximum amount of share capital the company is authorized to issue. It also mentions the division of this capital into shares and their respective nominal value.
Association Clause: It simply states that the subscribers wish to form a company and agree to become members of it, in accordance with the terms of the MOA.
Importance of Memorandum of Association:
Legal Requirement: The MOA is a legal requirement for the formation of a company. It must be filed with the Registrar of Companies during the incorporation process.
Constitutional Document: It serves as the company's constitutional document, defining its scope, powers, and limitations.
Protection of Members: It protects the interests of the company's members by clearly defining the objectives and limiting their liability.
External Communication: It provides clarity to external parties, such as investors, creditors, and regulatory authorities, regarding the company's objectives and powers.
https://seribangash.com/difference-public-and-private-company-law/
Binding Authority: The company and its members are bound by the provisions of the MOA. Any action taken beyond its scope may be considered ultra vires (beyond the powers) of the company and therefore void.
Amendment of MOA:
While the MOA lays down the company's fundamental principles, it is not entirely immutable. It can be amended, but only under specific circumstances and in compliance with legal procedures. Amendments typically require shareholder
Improving profitability for small businessBen Wann
In this comprehensive presentation, we will explore strategies and practical tips for enhancing profitability in small businesses. Tailored to meet the unique challenges faced by small enterprises, this session covers various aspects that directly impact the bottom line. Attendees will learn how to optimize operational efficiency, manage expenses, and increase revenue through innovative marketing and customer engagement techniques.
Affordable Stationery Printing Services in Jaipur | Navpack n PrintNavpack & Print
Looking for professional printing services in Jaipur? Navpack n Print offers high-quality and affordable stationery printing for all your business needs. Stand out with custom stationery designs and fast turnaround times. Contact us today for a quote!
1. PARP-1 Inhibitors in Oncology The Discovery and Development of CEP-9722, an Orally Active Prodrug for the Treatment of Cancer
2.
3.
4. Role of PARP-1 in DNA Repair N C N C A. de Murcia & M. de Murcia (1994) TIBS 19 , 172 DNA Damage ADP-ribose PARP Activation NAD + ATP
5. Role of PARP-1 in DNA Repair N C N C A. de Murcia & M. de Murcia (1994) TIBS 19 , 172 DNA Damage ADP-ribose PARP Activation NAD + ATP DNA damage repaired
6. Role of PARP-1 in DNA Repair N C N C A. de Murcia & M. de Murcia (1994) TIBS 19 , 172 DNA Damage ADP-ribose PARP Activation NAD + ATP DNA damage repaired Healthy cell
7. Role of PARP-1 in DNA Repair N C N C A. de Murcia & M. de Murcia (1994) TIBS 19 , 172 PARP Inhibitor DNA damage persists DNA Damage ADP-ribose PARP Activation NAD + ATP Damage repaired, Healthy cell
8. Role of PARP-1 in DNA Repair N C N C A. de Murcia & M. de Murcia (1994) TIBS 19 , 172 PARP Inhibitor DNA damage persists DNA Damage ADP-ribose PARP Activation NAD + ATP Damage repaired, Healthy cell Apoptosis, Cell death
9. PARP activity, NAD + , and ATP levels are interdependent Ha, H. C.; Neurobiology of Disease 7, 225–239 (2000)
13. o inc. radiation sensitivity of Chinese hamster V79 cells o Radiat Res; 126(3), 367 (1991) o potentiates TMZ and TP growth inhib. in human tumor cell lines o Clin Cancer Res; 6, 2860 (2000 ) Proof of Concept o Clinical trials candidate
14. o Potentiates TMZ, Cisplatin, radiation in syngeneic and xenograft tumor models o Clin Cancer Res; 13, 2728 (2007) o Completing Phase 2 trials w/TMZ o Irreversible inhibitor o Excellent Phase 2 results o First PARP inhibitor in Phase 3 trials o Failed primary endpoint o Cancelled Phase 3 trials for breast cancer o Commencing Phase 3 for ovarian cancer o Phase 1 trial for various cancers o Well tolerated o Commencing Phase 2 for Mantle Cell Lymphoma In the Clinic
19. PARP Inhibitor Discovery Flow In Vitro Cytotoxicity Assays (PARP inhibitors + Chemotherx.) In Vivo Chemo-Potentiation Studies GBMs /TMZ, HT-29/Irinotecan Significant shift in tumor versus normal cell kill versus chemotherx. alone In Vivo PAR Accumulation Assay No enhanced human myelotoxicity in vitro Biochemical efficacy in vivo Cmpd Scale-Up Significant potentiation of anti-tumor efficacy versus chemotherx. alone; acceptable systemic tolerability. Go/No Go Decision PK and Tolerability in Rodents rh PARP Inhibition Assay PC12 cells/H 2 O 2 insult Assay for Inhibition of NAD+ Depletion In vitro and in vivo evaluation on normal tissues; clinical chemistry and histopathology IC 50 < 50 nM 50% recovery @ < 1 uM > 90% max. recovery Criteria
53. Oncology Candace Burns Jennifer Grobelny Kathryn Hunter Sonya Pritchard Hugh Zhao Susan Jones-Bolin Bruce Ruggeri Acknowledgements Chung Ho Park Dandu Reddy Sankar Chatterjee Ron Bihovsky Gregory Wells Chemistry Mary Birchler Laura Gwinn Jean Husten Bruce Jones Biochemistry Seetha Murthy Damaris Rolon-Steele Kelli Zeigler Lisa Aimone Mark Ator Jim Diebold Ming Tao Derek Dunn Allison Zulli Bob Hudkins Fox Chase Cancer Center Andres Klein-Szanto
55. TMZ – Hydrolysis gives active form Temozolomide is not directly active but undergoes rapid nonenzymatic conversion at physiologic pH to the reactive compound 5-(3-methyltriazen-1-yl)-imidazole-4-carboxamide (MTIC). The cytotoxicity of MTIC is thought to be primarily due to alkylation of DNA. Alkylation (methylation) occurs mainly at the O 6 and N 7 positions of guanine.
56. 29th Annual J.P. Morgan Healthcare Conference January 10-12, 2011 Cephalon Oncology Pipeline
A small-molecule prodrug of CEP-8983, a novel 4-methoxy-carbazole inhibitor of the nuclear enzymes poly(ADP-ribose) polymerase (PARP) 1 and 2, with potential antineoplastic activity. Upon administration and conversion from CEP-9722, CEP-8983 selectively binds to PARP 1 and 2, preventing repair of damaged DNA via base excision repair (BER). This agent enhances the accumulation of DNA strand breaks and promotes genomic instability and apoptosis. CEP-8983 may potentiate the cytotoxicity of DNA-damaging agents and reverse tumor cell chemo- and radioresistance. PARP catalyzes post-translational ADP-ribosylation of nuclear proteins that signal and recruit other proteins to repair damaged DNA and can be activated by single strand breaks in DNA.
Inhibitors of PARP-1 have potential therapeutic utility in oncology through potentiation of the anti-tumor activity of radiation or chemotherapeutic DNA damaging agents. Until more recently, the potentiation observed in-vitro and in-vivo could not be unequivocally linked to inhibition of PARP due to limitations with respect to potency, selectivity, toxicity, and deliverability of compounds.
Poly (ADP-ribose) polymerase 1 (PARP-1) is a nuclear enzyme that catalyzes the synthesis of poly-ADP-ribose chains from NAD+ as part of the DNA repair process.
Poly (ADP-ribose) polymerase 1 (PARP-1) is a nuclear enzyme that catalyzes the synthesis of poly-ADP-ribose chains from NAD+ as part of the DNA repair process.
Poly (ADP-ribose) polymerase 1 (PARP-1) is a nuclear enzyme that catalyzes the synthesis of poly-ADP-ribose chains from NAD+ as part of the DNA repair process.
Poly (ADP-ribose) polymerase 1 (PARP-1) is a nuclear enzyme that catalyzes the synthesis of poly-ADP-ribose chains from NAD+ as part of the DNA repair process.
Poly (ADP-ribose) polymerase 1 (PARP-1) is a nuclear enzyme that catalyzes the synthesis of poly-ADP-ribose chains from NAD+ as part of the DNA repair process.
A model of PARP overactivation-mediated cytotoxicity. Reactive oxygen species (ROS) such as nitric oxide (NO z ), superoxide anion (O2--), peroxynitrite (ONOO2), or hydroxyl radical (OH z ) are generated during inflammation or ischemia–reperfusion. These ROS damage cellular molecules like proteins, lipids, and DNA. DNA damage activates PARP , synthesizing poly(ADP-ribose) polymer from its substrate b-nicotinamide adenine dinucleotide (NAD1). Limited amounts of DNA damage can be repaired by cellular DNA repair enzymes, and PARP, in this case, acts as a sensor for DNA damage, modulating the repair process. In apoptotic cell death, activated caspases cleave PARP protein to inhibit a futile DNA repair process and preserve cellular ATP, which is essential for the apoptotic process. Extensive DNA damage can lead to overactivation of PARP . With excessive activation of PARP, NAD1 is depleted, and in efforts to resynthesize NAD1, ATP is also depleted. Hence, necrotic cell death involves energy loss. Depletion of ATP can transform an ongoing apoptotic process into necrosis with intracellular ATP levels regulating the mode of cell death.
- Colorectal Carcinoma with Irinotecan #2 choice HNSCC with radiotherapy #3 choice
More recent competition?
Screening of Cephalon’s internal library identified pyrrolocarbazole CEP-3498 as a potent inhibitor of PARP-1 with an IC50 value of 35 nM, although with poor cellular permeability and solubility. The chemistry objective was to improve the enzyme and cell potency, and the physical-chemical properties to allow either oral or i.v. delivery in the clinic.
Key H-bond interactions between imide C=O and N-H groups with backbone N-H and C=O of Gly863 ; H-bond between indole-NH and C=O of Glu988 side-chain; aromatic pi-stacking of aryl rings of Tyr896 and Tyr907 with B and D rings of the carbazole, resp.; cyclopentyl ring fits closely into fold formed by Lys861 side-chain, Ala898, Trp861, and Asn987; open pocket around 3- and 4-positions of the inhibitor provided opportunity for SAR development.
Key H-bond interactions between lactam C=O and N-H groups with backbone N-H and C=O of Gly863 ; H-bond between indole-NH and C=O of Glu988 side-chain; aromatic pi-stacking of aryl rings of Tyr896 and Tyr907 with B and D rings of the carbazole, resp.; cyclopentyl ring fits closely into fold formed by Lys861 side-chain, Ala898, Trp861, and Asn987; open pocket around 3- and 4-positions of the inhibitor provided opportunity for SAR development.
But with limited opportunity elsewhere on the core template, the aryl group was retained for further SAR development.
1-Aza analog CEP-9397 modeled with PARP-1 active site, postulated as more potent due to an additional H-bond between the 1-aza group and the amine side chain of Met890.