This document provides an overview of parasitology, including definitions of key terms like parasite, host, symbiosis, parasitism, and vectors. It describes different types of parasites like ecto-parasites, endoparasites, temporary vs permanent parasites. It also covers classes of hosts, portals of entry, life cycles, pathogenicity, and immune responses to parasites. Laboratory diagnosis methods are discussed, especially demonstration of parasites in blood samples. In summary, the document defines important concepts in parasitology and provides details on parasite and host relationships, life cycles, disease pathogenesis, and diagnostic approaches.

1. Introductory Parasitology
Submitted by:
Dr. Rekha Sansanwal
Assistant Professor
Department of Microbiology
CBLU, Bhiwani, Haryana

2. Introduction to Parasitology
• Parasitology is the study of parasites, their hosts, and the
relationship between them.
• Parasitology is the area of biology concerned with the
phenomenon of dependence of one living organism on another.
• Medical parasitology deals with the parasites which infect
man, the diseases they produce, the response generated by him
against them and various methods of diagnosis and prevention.

3. • Parasite-A living organism which receives nourishment and shelter from
another organism where it lives.
• Host-An organism which harbors the parasite.
• Symbiosis-It is an association in which both are so dependent upon each
other that one cannot live without the help of the other. None of the partner
suffers any harm from the association.
• Commensalism-An association in which the parasite only is deriving
benefit without causing injury to its host. A commensal is capable of
leading an independentlife.
• Parasitism-An association in which the parasite derives benefit and the
host gets nothing in return but always suffers some injury. A parasite has
lost its power of independentlife.
• Zoonosis- It means a disease of animals. Leishmaniasis, trypanosomiasis,
trichinelliasis and echinococcosis.

4. Classes of Parasites
i. Ecto-parasite (Ectozoa): Lives outside on the surface of the body of
the host.
ii. Endoparasite-lives inside the body of the host, in the blood, tissues,
body cavities, digestive tract and other organs.
iii. Temporary parasite-Visits its host for a short period.
iv. Permanent parasite-leads a parasitic life throughtout the whole
period of its life.
v. Facultative parasite-Lives a parasitic life when opportunityarises.
vi. Obligatoryparasite-Cannotexist without a parasitic life.
vii. OccasionalorAccidental Parasite-Attacks an unusal host.
viii.Wandering or Aberrant parasite-Happens to reach a place where it
cannot live.
Free living- The term free living describes the non parasitic stages of
existence which are lived independently of a host. e.g. hookwarm have active
free living stages in the soil.

5. Classes of Hosts
• DefinitiveHost - Eitherharbors the adult stage of the parasite Or Where
the parasite utilizes the external method of reproduction.
• In majority of human parasitic infections, man is the definitive host.
• In malaria and hydatid disease, man acts as the intermediate host.
• Intermediatehost - It harborsthe larval stages of the parasite. In some
cases larval developmentsare completedin two different intermediate
hosts. These are referred as first and second intermediatehosts respectively.
• ParatenicHost- ( A carrier and transporthost)-Ahost where the parasite
remains viable without further development.
• Reservoirhost-It is a host which harbor the parasite and serves as an
important source of infection to other susceptiblehosts. Epidemiologically,
reservoir hosts are important in the controlof parasitic disease.

6. Vector
• Vector-A vector is an agent, usually an insect, that transmits an infection
from one host to another.
• Mechanical vector -This is a vector which assists in the transfer of
parasitic forms between hosts but is not essential in the life cycle of the
parasite. e.g. a housefly that transfers amoebic cysts from infected feaces to
food that is eaten by humans.
• Sources of infection-
1. Contaminatedsoil and water
2. Freshwater fishes- Diphyllobothriumlatumand Clonorchis sinensis.

7. Portal of entry into the body
1. Mouth- Commonest portal of entry of the parasites is oral through
contaminatedfood, water, soiled fingers or fomites.
2. Skin- Entry through skin is another important portal of entry of parasites.
Infection with A.duodenale, N.americanus and S.stercoralis is acquired
when filariform larvae of these nematodes penetrate the unbroken skin of
an individual walking over faecally contaminated soil. Schistosomiasis
caused by S. haematobium, S. mansoni and S. japonicum is acquired
when the cercarial larvae, in water, penetratethe skin.
3. Sexual contact-Trichomonas vaginalisis transmitted by sexual contact.
4. Congenital- Infection with T.gondii and Plasmodium spp. May be
transmitted from mother to fetus transplacentally.
5. Inhalation
6. Iatrogenic infection- Malaria parasites may be transmitted by transfusion
of blood from the donor with malaria containing asexual forms of
erythrocytic schizogony. This is known as trophozoite induced malaria or
transfusion malaria. Malaria parasites may also be transmitted by the use
of contaminatedsyringes and needles. This may occur in drug addicts.

8. Life cycle of human parasites
Pathogenicity-A parasite may live in or on the tissues of its host without causing evident harm.
– In majority of cases the parasite has the capacity to produce damage.
Following are the ways in which the damage may be produced by the parasites:
A. Traumatic damage-
– Physical damage is produced by entry of filariform larvae of S. stercoralis, A. duodenale and
N.americanus and cercarial larvae of S.haematobium, S.mansoni and S.japonicum into the skin.
– Migration of several helminthic larvae through traumatic damage of pulmonary capillaries
leading to extravasation of blood into the lung. Damage in cerebral, retinal or renal capillaries
may lead to serious injury.
– Eggs of S.haematobium and S.mansoni cause extensive damage with haemarrhage as they
escape from vesical and mesenteric venules, respectively, into the lumen of the urinary bladder
and the intestinal canal.
– Attachment of hookwarms to the intestinal wall results in traumatic damage of the villi and
oozing of blood at the site of attachment. Large worms, such ad A.lumbricoides and T.saginata
may produce intestinal obstruction.
– Ascaris may occlude lumen of the appendix or common bile duct, may cause performation of
the intestinal wall. They may penetrate into the parenchyma of the liver and the lungs.

9. • Lytic necrosis-
– E.histolytica secretes lytic enzyme which lyses tissues for its nutritional
needs. It helps it to penetrate into the tissues of the colon and extraintestinal
viscera.
– Obligate intracellular parasites e.g. Plasmodium spp., Leishmania Spp.,
Trypanosoma cruzi and Toxoplasma gondii cause necrosis of parasitized
host cells during their growth and multiplication.
– Competition for specific nutrients- Diphyllobothrium latum competes with
the host for vitamin B12 leading to parasite induced pernicious anaemia.
Inflammatory reaction-Most of the parasites provoke cellular proliferation
and infiltration at the site of their location.
– In E.histolytica may produce inflammation of the large intestine leading to
the formation of amoebic granuloma or amoeboma.
– Parasitization of fixed macrophages in the spleen, bone marrow, and lymph
nodes by L.donovani causes proliferationof reticuloendothelialcells.

10. • Allergic manifestations-
– In certain helminthicinfections, the normal secretions and excretions of
the growing larvae and the productsliberated from dead parasites may
give rise to various allergic manifestations.
– Schistosomescause cercarial dermatitis and eosinophilia.
– D.medinensisand T.spiralisinfections cause urticaria and eosinophilia.
– Rupture of the hydatid cyst may precipitateanaphylaxis.
• Neoplasia-
– The parasitic infection may contributeto the development of neoplastic
growth. C.sinensisand Opisthorchisviverrini have been associated
with cholangiocarcinoma and S. haematobiumwith vesical carcinoma.
• Secondary infection-
– In some helminthic infections (Strongyloidiasis, trichinosis and
ascariasis), the migrating larvae may carry bacteriaand viruses from
the intestine to the blood and tissues leading to secondaryinfection.
– Immunity in parasitic infections. Protozoaare small and multiply
within their vertebrate host, inside cells.

11. • Parasite also elicit both humoral as well as cellular responses. But immunological
protection against parasitic infections is much less efficient than it is against bacterial
and viral infections.
• Parasites are large and more complex structurally and antigenically so that immune
system may not be able to mount response against the protective antigens.
• Many protozoa parasites (e.g. Leishmania spp. and T.gondii) are intracellular. This
protects them from immunological attack.
• Many parasites, both protozoa and helminths, live inside the intestines. This location
limits the efficiency of immunological attack.
• T. brucei gambiense and T.b.rhodesience exhibit antigenic variations within the host.
Many nematodes have a cuticle which is antigenically inert and evokes little immune
response.
• L.donovani causes extensive damage to the reticuloendothelial system thus leading to
immunological tolerance.
• E.vermicularis does not breach the integrity of gut wall, thus immune system is not
stimulated. In most of the parasitic infections, immunity lasts only till original infection
remains active. This is known as concomitant immunity. (Premunition or infection
immunity)

12. • The protective immune response to parasitic infections has four arms-
– CytotoxicT (Tc) cells
– Natural killer (NK) cells
– Activated macrophages
– Antibody(Produced by B-cells)
• The main classes of antibodies(Immunoglobulins) producedare IgM, IgG,
and IgE.
• IgM appears first and denotesrecent infection.
• IgG antibodiesare usually the most abundanttype in parasitic infections.
• Helminths and ectoparasitesalso provoke high titres of IgE antibodies.
Laboratory diagnosis
1. Demonstration of parasite
2. Immunodiagnosis
3. Molecularbiological methods

13. 1. Demonsration of parasites:
A. Blood-
In those parasitic infections,where the parasite itself, or in any stage of its
development, circulates in the blood stream, the examination of blood film
forms the main procedure for specific diagnosis
e.g. Demonstration of Plasmodium spp.
Babesia spp. inside the erythrocytes
L.donovani inside monocytes
Trypomastigotes of T.b.gambiense, T.b.rhodesience, T.cruzi., W.bancrofti and
B.malayi in the blood.
B. Stool-
Examination of stool is important for the diagnosis of intestinal
parasitic infections and helminthic infections of the biliary tract
Eggs are discharged in the intestine. In the protozoal infections,
trophozoites(during active phase) and cysts (during chronic phase) of
E.histolytica, G.lamblia and B.coli can be demonstrated by wet mount
of stool in normal saline and Lugol’s iodine.
In helminthic infections eggs, larvae and adult worms may be
demonsrated.
Demonstration of parasites in the stools confirm the diagnosis. It is the
gold standard in the diagnosis of intestinal parasitic infections.
C. Perianaland perineal skin scrapings-
May show the eggs or adult worms of E.vermicularis.

14. D. Urine
Urine is useful in establishing the parasitological diagnosis when the parasite localises in the urinary
tract. Eggs of S.haematobium and trophozoites of T.vaginalis may be demonstrated in the urine.
In case of chyluria caused by W.bancrofti, microfilariae are often demonstrated in chylous urine.
E. Genital specimens
Trophozoites of T.vaginalis may be demonstrated in the vaginal and urethral discharge and in the
prostatic secretions.
F. Cerebrospinal fluid ( CSF)
Trypomastigotes of T.brucei may be demonstrated in the CSF.
G. Sputum
Eggs of Paragonimus wertermani may be demonstrated in the sputum specimen. Rarely, migrating
larvae of A. lumbricoides, S.stercoralis, A.duodenale and N.americanus, and trophozoites of E.histolytica
may be found in the sputum.
H. Tissue biopsy and aspiration
Scolices and brood capsules may be demonstrated in the fluid aspirated from hydatid cyst.
Amastigote forms of L.donovani may be demonstated inside the reticuloendothelial cells in the aspirates
of spleen, bone marrow, liver and lymph nodes.
Larvae of T.spiralis,T.solium and T.multiceps may be demonstrated in the muscle biopsy.
Trophozoites of G.lamblia may be demonstrated in the bile aspirated from duodenum by intubation.
Trophozoites of E.histolytica may be demonstrated in pus aspirated from amoebic liver abscess and in
the necrotic tissue obtained from the base of the ulcers in the large intestine.

15. • Culture-Some parasites like E.histolytica and G.lamblia in stool and
Leishmania spp. and Trypanosoma spp. in bloodcan be cultured in the
laboratory. Cultureof parasites is particularlyuseful when the number of
parasites in the specimens is too small.
• Animal inoculation-It is useful in the detectionof T.gondii and Babesia
spp. in the clinical specimens.
Immunodiagnosis-two type of tests are available
1. Skin test
2. Serological tests
1. Skin test-These tests are performed by intradermal injection of parasitic
antigens.
 Immediate hypersensitivityreaction-It reveals erythema and
indurationafter 30 minutes of injection. This reaction is seen in
cases of hydatid disease, filariasis, schistosomiasis, ascariasis and
strongyloidiasis.
 Delayed hypersensitivityreaction-It reveals erythema and
indurationafter 48 hoursof injection. This reaction is seen in cases
of leishmaniasis, trypanosomiasis, toxoplasmosis and amoebiasis.

16. 2. Serological tests- These tests detect antibodies or antigens in the patient serum
and other clinical specimens.
Molecular biological methods-These include DNA probes and polymerase
chain reactions (PCR).
– DNAprobes-
DNA probe is a radiolabelled or chromogenically labelled piece of single
stranded DNA complementary to a segment of parasitic genome. It is
unique to a particular parasitic strain , species and genus.
Specific probe is added to the clinical specimen. If the specimen contains
the parasitic DNA, probe will hybridize with it, which can be detected.
DNA probes are available for the detection of the infection with
P.falciparum, W.bancrofti.
– Polymerase chainreaction-
 PCR is a DNA amplification system that allows molecular biologist to
produce microgram quantities of DNA from picogram amounts of
starting material.
It has been employed to detect faecal antigens for the diagnosis of
intestinal amoebiasis, giardiasis and other intestinal parasitic infections.

17. Different types of AnimalAssociations
• Organisms in a community influence each other either directly or indirectly.
• Under natural conditions, an organism never lives in absolute isolation and hence
must interact for the vital processes of life including growth and reproduction.
• Organisms biologically influence individuals of same species (intraspecific
interaction) e.g. pollination, and grazing or may involve different species
(interspecific interaction) e.g. parasitism.
• They interact with other ecological factors of the environment that ultimately
leads to the proper functioningof the ecosystem.
• Therefore, these complex interactions are indeed fundamental to the survival of an
organism.
• The interdependencies between species lead to spatial and physiological
relationships and at times species interactions results in widely separated
individuals with taxonomical relationships, for instance bacteria and plants,
fungus and plants, bacteria and humans etc.
• The interactions may be neutral, beneficial or harmful and can be further classified
on the basis of the duration of relationship grading from casual to permanent and
also on the basis of the strength or mechanism of interactionsbetween organisms.

18. • These classifications were further elaborated by Burkholder, 1952, who proposed effects
of “0”, “+” and “–” and their combinations are grouped into nine types of relationships.
– “0” indicates non-significant interaction between species,
– “+” indicates growth, survival or the population has benefited
– “-” indicates adverse effects of one over the other, by inhibiting growth, survival or other
attributes.
Positive interactions -A positive interaction is a relationship where the interacting organisms
derive benefits from one another and neither is harmed.
A. Mutualism,
B. Commensalisms
C. Protocooperation
Negative interactions - Negative interactions are the relationships where one of the
interacting organism unfavourably affects the survival, growth and other population attributes
of the other directly or indirectly.
They may compete for the common resources like food, water, space, sex etc. Negative
interactions have been further sub divided into four types:
A. Competition
B. Predation
C. Antibiosis
D. Parasitism.

20. Thank you