1. Researchers studied the effects of water isotopes and osmotic stress on the gliding motility of kinesin-1 molecular motors. They found that heavy hydrogen water and the osmolyte betaine reduced the gliding speed of microtubules.
2. The differences seen between surface passivation conditions and over time in previous experiments are likely due to the gradual warming of the microscope from the lamp.
3. Automated image analysis was developed to track microtubules and characterize their speeds, allowing high throughput analysis of experimental conditions.
Thermoluminescence in Pure and Nd+3 + K+ Doped Lead Germanate Single CrystalsAI Publications
Pure lead germanate was grown by Zochralski’stechnique.Nd+3 + K+ doped lead germanate was grown for comparative studies. Thermoluminescence studies were conducted on pure lead germanate and Nd+3 + K+ doped lead germanate. Thermoluminescence in pure lead germanate single crystals (LG) and Nd+3 + K+ doped lead germanate single crystals (DDLG) has been measured in terms of photomultiplier output current on Thermoluminescence Set Up. Thermoluminescence studies of LG revealed an increasing trend of Thermoluminescence output, with increasing temperature and sharp consistent maxima at 50°C and 100°C. TL studies of DDLG revealed a decreasing trend of Thermoluminescence output, with increasing temperature and a sharp consistent peak at 100°C and 120°C. It was further noted that doping increased Thermoluminescence output. Thermoluminescence in ferroelectric lead germanate was attributed to change in polarization with change in temperature and creation of defect states.
Thermoluminescence in Pure and Nd+3 + K+ Doped Lead Germanate Single CrystalsAI Publications
Pure lead germanate was grown by Zochralski’stechnique.Nd+3 + K+ doped lead germanate was grown for comparative studies. Thermoluminescence studies were conducted on pure lead germanate and Nd+3 + K+ doped lead germanate. Thermoluminescence in pure lead germanate single crystals (LG) and Nd+3 + K+ doped lead germanate single crystals (DDLG) has been measured in terms of photomultiplier output current on Thermoluminescence Set Up. Thermoluminescence studies of LG revealed an increasing trend of Thermoluminescence output, with increasing temperature and sharp consistent maxima at 50°C and 100°C. TL studies of DDLG revealed a decreasing trend of Thermoluminescence output, with increasing temperature and a sharp consistent peak at 100°C and 120°C. It was further noted that doping increased Thermoluminescence output. Thermoluminescence in ferroelectric lead germanate was attributed to change in polarization with change in temperature and creation of defect states.
2009 September Kinesin Talk at UNM ChemistrySteve Koch
Talk given by Steve at the Unviversity of New Mexico Chemistry Department on September 11, 2009. It is mostly still an introduction to our kinesin project, but now I'm able to include the latest results from gliding motility assay, tracking software, and stochastic kinetics simulation.
High Precision, Not High Energy: Using Atomic Physics to Look Beyond the Stan...Chad Orzel
Second of two lectures on using atomic physics techniques to look for exotic physics, given at the Nordita Workshop for Science Writers on Quantum Theory. This one focusses on the measuring of tiny frequency shifts using techniques developed for atomic clocks.
Effective Viscosity of Actively Swimming Algae Suspensionslucascaretta
As the demand for energy soars, the introduction of algae biofuels as a renewable source of energy is receiving much attention. Suspensions of these actively swimming microorganisms exhibit an effective viscosity that may depend on volume fraction, cell shape, and the nature of locomotion (e.g. ``pushers\'\' vs. ``pullers\'\'). Here we report experimental measurements of shear viscosity for suspensions of unicellular green algae (Dunaliella primolecta, a biflagellated ``puller\'\'). We use a cone-and-plate rheometer to measure the dynamic shear viscosity for both motile and non-motile suspensions of D. primolecta at concentrations ranging from 0.1% to 10% of volume fraction. Viscosity increases with concentration for both cases, but the active suspensions of ``pullers\'\' have a comparatively higher effective viscosity than passive suspensions. This observation confirms recently proposed theories that predict higher effective viscosity for ``puller\'\' suspensions compared to non-motile suspensions. Our locomotion study reveals that motile algal cells prefer to align and migrate in the direction of positive shear flow vorticity. It is our belief that such a shear-induced response of the algal cells impacts the resulting effective shear viscosity.
Efficient time-series forecasting of nuclear reactions using swarm intellige...IJECEIAES
In this research paper, we focused on the developing a secure and efficient time-series forecasting of nuclear reactions using swarm intelligence (SI) algorithm. Nuclear radioactive management and efficient time series for casting of nuclear reactions is a problem to be addressed if nuclear power is to deliver a major part of our energy consumption. This problem explains how SI processing techniques can be used to automate accurate nuclear reaction forecasting. The goal of the study was to use swarm analysis to understand patterns and reactions in the dataset while forecasting nuclear reactions using swarm intelligence. The results obtained by training the SI algorithm for longer periods of time for predicting the efficient time series events of nuclear reactions with 94.58 percent accuracy, which is higher than the deep convolution neural networks (DCNNs) 93% accuracy for all predictions, such as the number of active reactions, to see how the results can improve. Our earliest research focused on determining the best settings and preprocessing for working with a certain nuclear reaction, such as fusion and fusion task: forecasting the time series as the reactions took 0-500 ticks being trained on 300 epochs.
DNA sequencing: rapid improvements and their implicationsJeffrey Funk
these slides analyze the rapid improvements in DNA sequencers and the implications for these rapid improvements for drug discovery, new crops, materials creation, and new bio-fuels. Many of the rapid improvements are from "reductions in scale." As with integrated circuits, reducing the size of features on DNA sequencers has enabled many orders of magnitude improvements in them. Unlike integrated circuits, the improvements are also due to changes in technology. For example, changes from pyrosequencing to semiconductor and nanopore sequencing have also been needed to achieve the reductions in scale. Second, pyrosequencing also benefited from improvements in lasers and camera chips.
ATEAS V1(2):: American Transactions on Engineering & Applied Sciencesdrboon
Research from American Transactions on Engineering & Applied Sciences:: A Detailed Analysis of Capillary Viscometer
Fuzzy Logic Modeling Approach for Risk Area Assessment for Hazardous Materials Transportation
Computer Modeling of Internal Pressure Autofrettage Process of a Thick-Walled Cylinder with the Bauschinger Effect
Types of Media for Seeds Germination and Effect of BA on Mass Propagation of Nepenthes mirabilis Druce
Numerical Analysis of Turbulent Diffusion Combustion in Porous Media
Production of Hydrocarbons from Palm Oil over NiMo Catalyst
High Throughput Investigation of EC Coupling in Isolated Cardiac MyocytesInsideScientific
During this webinar sponsored by IonOptix, Michiel Helmes, PhD discusses recent advancements in instrumentation that address the shortfalls of low throughput EC coupling characterization. Specifically, Dr. Helmes explains the technology behind faster data acquisition and analysis, as well as improvements to the studies that offer more data acquisition fidelity, and automated data collection. He offers insights into best-practices for proper EC coupling measurement and highlight improvements to data handling, namely faster, automated data analysis.
Background: Measuring and analyzing calcium and contractility in isolated cardiomyocytes offers important insights into cardiac function. However, traditional methods of obtaining EC coupling data are somewhat limited to lower throughput — for many applications, particularly drug discovery research, this presents a big challenge. Additionally, low throughput data acquisition and analysis may lack the statistical power necessary to fully resolve differences, or changes, in cardiac function. Isolated myocytes can behave heterogeneously, thus greater sample numbers are essential for accurate and reliable modeling of cardiac behavior.
Observation of Bose–Einstein condensates in an Earth-orbiting research labSérgio Sacani
Quantum mechanics governs the microscopic world, where low mass and momentum
reveal a natural wave–particle duality. Magnifying quantum behaviour to
macroscopic scales is a major strength of the technique of cooling and trapping
atomic gases, in which low momentum is engineered through extremely low
temperatures. Advances in this feld have achieved such precise control over atomic
systems that gravity, often negligible when considering individual atoms, has
emerged as a substantial obstacle. In particular, although weaker trapping felds
would allow access to lower temperatures1,2
, gravity empties atom traps that are too
weak. Additionally, inertial sensors based on cold atoms could reach better
sensitivities if the free-fall time of the atoms after release from the trap could be made
longer3
. Planetary orbit, specifcally the condition of perpetual free-fall, ofers to lift
cold-atom studies beyond such terrestrial limitations. Here we report production of
rubidium Bose–Einstein condensates (BECs) in an Earth-orbiting research laboratory,
the Cold Atom Lab. We observe subnanokelvin BECs in weak trapping potentials with
free-expansion times extending beyond one second, providing an initial
demonstration of the advantages ofered by a microgravity environment for
cold-atom experiments and verifying the successful operation of this facility. With
routine BEC production, continuing operations will support long-term investigations
of trap topologies unique to microgravity4,5
, atom-laser sources6
, few-body physics7,8
and pathfnding techniques for atom-wave interferometry9–12
Toward Real-Time Analysis of Large Data Volumes for Diffraction Studies by Ma...EarthCube
Talk at the EarthCube End-User Domain Workshop for Rock Deformation and Mineral Physics Research.
By Martin Kunz, Lawrence Berkeley National Laboratory
2011 NSF CAREER_Steve Koch Full Project Description Steve Koch
This is the full Project Description for my 2011 NSF CAREER proposal. As I described on my blog, I am disappointed in the unfinished product, mostly because I still think the proposed research is important, exciting, and achievable by my lab. ( http://stevekochresearch.blogspot.com/2011/08/2011-nsf-career-proposal-ugh-failures.html )
Here are links to prior years' proposals, which were declined:
* 2009 http://www.scribd.com/doc/17548381/2009-ProposalCAREER-SingleMolecule-Analysis-of-Genomic-DNA-and-Chromatin-in-Eukaryotic-Transcription
* 2008 http://www.scribd.com/doc/10196076/2008-NSF-CAREERproposal-Only
2009 September Kinesin Talk at UNM ChemistrySteve Koch
Talk given by Steve at the Unviversity of New Mexico Chemistry Department on September 11, 2009. It is mostly still an introduction to our kinesin project, but now I'm able to include the latest results from gliding motility assay, tracking software, and stochastic kinetics simulation.
High Precision, Not High Energy: Using Atomic Physics to Look Beyond the Stan...Chad Orzel
Second of two lectures on using atomic physics techniques to look for exotic physics, given at the Nordita Workshop for Science Writers on Quantum Theory. This one focusses on the measuring of tiny frequency shifts using techniques developed for atomic clocks.
Effective Viscosity of Actively Swimming Algae Suspensionslucascaretta
As the demand for energy soars, the introduction of algae biofuels as a renewable source of energy is receiving much attention. Suspensions of these actively swimming microorganisms exhibit an effective viscosity that may depend on volume fraction, cell shape, and the nature of locomotion (e.g. ``pushers\'\' vs. ``pullers\'\'). Here we report experimental measurements of shear viscosity for suspensions of unicellular green algae (Dunaliella primolecta, a biflagellated ``puller\'\'). We use a cone-and-plate rheometer to measure the dynamic shear viscosity for both motile and non-motile suspensions of D. primolecta at concentrations ranging from 0.1% to 10% of volume fraction. Viscosity increases with concentration for both cases, but the active suspensions of ``pullers\'\' have a comparatively higher effective viscosity than passive suspensions. This observation confirms recently proposed theories that predict higher effective viscosity for ``puller\'\' suspensions compared to non-motile suspensions. Our locomotion study reveals that motile algal cells prefer to align and migrate in the direction of positive shear flow vorticity. It is our belief that such a shear-induced response of the algal cells impacts the resulting effective shear viscosity.
Efficient time-series forecasting of nuclear reactions using swarm intellige...IJECEIAES
In this research paper, we focused on the developing a secure and efficient time-series forecasting of nuclear reactions using swarm intelligence (SI) algorithm. Nuclear radioactive management and efficient time series for casting of nuclear reactions is a problem to be addressed if nuclear power is to deliver a major part of our energy consumption. This problem explains how SI processing techniques can be used to automate accurate nuclear reaction forecasting. The goal of the study was to use swarm analysis to understand patterns and reactions in the dataset while forecasting nuclear reactions using swarm intelligence. The results obtained by training the SI algorithm for longer periods of time for predicting the efficient time series events of nuclear reactions with 94.58 percent accuracy, which is higher than the deep convolution neural networks (DCNNs) 93% accuracy for all predictions, such as the number of active reactions, to see how the results can improve. Our earliest research focused on determining the best settings and preprocessing for working with a certain nuclear reaction, such as fusion and fusion task: forecasting the time series as the reactions took 0-500 ticks being trained on 300 epochs.
DNA sequencing: rapid improvements and their implicationsJeffrey Funk
these slides analyze the rapid improvements in DNA sequencers and the implications for these rapid improvements for drug discovery, new crops, materials creation, and new bio-fuels. Many of the rapid improvements are from "reductions in scale." As with integrated circuits, reducing the size of features on DNA sequencers has enabled many orders of magnitude improvements in them. Unlike integrated circuits, the improvements are also due to changes in technology. For example, changes from pyrosequencing to semiconductor and nanopore sequencing have also been needed to achieve the reductions in scale. Second, pyrosequencing also benefited from improvements in lasers and camera chips.
ATEAS V1(2):: American Transactions on Engineering & Applied Sciencesdrboon
Research from American Transactions on Engineering & Applied Sciences:: A Detailed Analysis of Capillary Viscometer
Fuzzy Logic Modeling Approach for Risk Area Assessment for Hazardous Materials Transportation
Computer Modeling of Internal Pressure Autofrettage Process of a Thick-Walled Cylinder with the Bauschinger Effect
Types of Media for Seeds Germination and Effect of BA on Mass Propagation of Nepenthes mirabilis Druce
Numerical Analysis of Turbulent Diffusion Combustion in Porous Media
Production of Hydrocarbons from Palm Oil over NiMo Catalyst
High Throughput Investigation of EC Coupling in Isolated Cardiac MyocytesInsideScientific
During this webinar sponsored by IonOptix, Michiel Helmes, PhD discusses recent advancements in instrumentation that address the shortfalls of low throughput EC coupling characterization. Specifically, Dr. Helmes explains the technology behind faster data acquisition and analysis, as well as improvements to the studies that offer more data acquisition fidelity, and automated data collection. He offers insights into best-practices for proper EC coupling measurement and highlight improvements to data handling, namely faster, automated data analysis.
Background: Measuring and analyzing calcium and contractility in isolated cardiomyocytes offers important insights into cardiac function. However, traditional methods of obtaining EC coupling data are somewhat limited to lower throughput — for many applications, particularly drug discovery research, this presents a big challenge. Additionally, low throughput data acquisition and analysis may lack the statistical power necessary to fully resolve differences, or changes, in cardiac function. Isolated myocytes can behave heterogeneously, thus greater sample numbers are essential for accurate and reliable modeling of cardiac behavior.
Observation of Bose–Einstein condensates in an Earth-orbiting research labSérgio Sacani
Quantum mechanics governs the microscopic world, where low mass and momentum
reveal a natural wave–particle duality. Magnifying quantum behaviour to
macroscopic scales is a major strength of the technique of cooling and trapping
atomic gases, in which low momentum is engineered through extremely low
temperatures. Advances in this feld have achieved such precise control over atomic
systems that gravity, often negligible when considering individual atoms, has
emerged as a substantial obstacle. In particular, although weaker trapping felds
would allow access to lower temperatures1,2
, gravity empties atom traps that are too
weak. Additionally, inertial sensors based on cold atoms could reach better
sensitivities if the free-fall time of the atoms after release from the trap could be made
longer3
. Planetary orbit, specifcally the condition of perpetual free-fall, ofers to lift
cold-atom studies beyond such terrestrial limitations. Here we report production of
rubidium Bose–Einstein condensates (BECs) in an Earth-orbiting research laboratory,
the Cold Atom Lab. We observe subnanokelvin BECs in weak trapping potentials with
free-expansion times extending beyond one second, providing an initial
demonstration of the advantages ofered by a microgravity environment for
cold-atom experiments and verifying the successful operation of this facility. With
routine BEC production, continuing operations will support long-term investigations
of trap topologies unique to microgravity4,5
, atom-laser sources6
, few-body physics7,8
and pathfnding techniques for atom-wave interferometry9–12
Toward Real-Time Analysis of Large Data Volumes for Diffraction Studies by Ma...EarthCube
Talk at the EarthCube End-User Domain Workshop for Rock Deformation and Mineral Physics Research.
By Martin Kunz, Lawrence Berkeley National Laboratory
2011 NSF CAREER_Steve Koch Full Project Description Steve Koch
This is the full Project Description for my 2011 NSF CAREER proposal. As I described on my blog, I am disappointed in the unfinished product, mostly because I still think the proposed research is important, exciting, and achievable by my lab. ( http://stevekochresearch.blogspot.com/2011/08/2011-nsf-career-proposal-ugh-failures.html )
Here are links to prior years' proposals, which were declined:
* 2009 http://www.scribd.com/doc/17548381/2009-ProposalCAREER-SingleMolecule-Analysis-of-Genomic-DNA-and-Chromatin-in-Eukaryotic-Transcription
* 2008 http://www.scribd.com/doc/10196076/2008-NSF-CAREERproposal-Only
This is a summary I gave at group meeting today on what I'd learned about D2O (aka "heavy water" aka "deuterium oxide") and its effects on biochemistry/biophysics of enzymes and proteins.
Here are the slides describing talents and hedgehog concepts in the context of students' future careers. It's the background to our final assignment for the semester: http://openwetware.org/wiki/User:Steven_J._Koch/Talents_assignment
Discussion of dispersion and rainbows. Also some cool photos of blackbody and fluorescent spectra from Tom Decaro and Analisa Goodman as part of the homework question.
08 Feb 17 Light, Electron E Levels Actual PresentedSteve Koch
Introduction to electromagnetic radiation and light. Viewing atomic spectra with diffraction gratings. Optical tweezers (cool example of light having momentum).
Smart TV Buyer Insights Survey 2024 by 91mobiles.pdf91mobiles
91mobiles recently conducted a Smart TV Buyer Insights Survey in which we asked over 3,000 respondents about the TV they own, aspects they look at on a new TV, and their TV buying preferences.
DevOps and Testing slides at DASA ConnectKari Kakkonen
My and Rik Marselis slides at 30.5.2024 DASA Connect conference. We discuss about what is testing, then what is agile testing and finally what is Testing in DevOps. Finally we had lovely workshop with the participants trying to find out different ways to think about quality and testing in different parts of the DevOps infinity loop.
Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But there’s more:
In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
But—if the “Reject” button is pushed, colleagues will be alerted via Slack message
Join us to learn more about this new, human-in-the-loop capability, brought to you by Integration Service connectors.
And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
Builder.ai Founder Sachin Dev Duggal's Strategic Approach to Create an Innova...Ramesh Iyer
In today's fast-changing business world, Companies that adapt and embrace new ideas often need help to keep up with the competition. However, fostering a culture of innovation takes much work. It takes vision, leadership and willingness to take risks in the right proportion. Sachin Dev Duggal, co-founder of Builder.ai, has perfected the art of this balance, creating a company culture where creativity and growth are nurtured at each stage.
PHP Frameworks: I want to break free (IPC Berlin 2024)Ralf Eggert
In this presentation, we examine the challenges and limitations of relying too heavily on PHP frameworks in web development. We discuss the history of PHP and its frameworks to understand how this dependence has evolved. The focus will be on providing concrete tips and strategies to reduce reliance on these frameworks, based on real-world examples and practical considerations. The goal is to equip developers with the skills and knowledge to create more flexible and future-proof web applications. We'll explore the importance of maintaining autonomy in a rapidly changing tech landscape and how to make informed decisions in PHP development.
This talk is aimed at encouraging a more independent approach to using PHP frameworks, moving towards a more flexible and future-proof approach to PHP development.
Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
Essentials of Automations: Optimizing FME Workflows with ParametersSafe Software
Are you looking to streamline your workflows and boost your projects’ efficiency? Do you find yourself searching for ways to add flexibility and control over your FME workflows? If so, you’re in the right place.
Join us for an insightful dive into the world of FME parameters, a critical element in optimizing workflow efficiency. This webinar marks the beginning of our three-part “Essentials of Automation” series. This first webinar is designed to equip you with the knowledge and skills to utilize parameters effectively: enhancing the flexibility, maintainability, and user control of your FME projects.
Here’s what you’ll gain:
- Essentials of FME Parameters: Understand the pivotal role of parameters, including Reader/Writer, Transformer, User, and FME Flow categories. Discover how they are the key to unlocking automation and optimization within your workflows.
- Practical Applications in FME Form: Delve into key user parameter types including choice, connections, and file URLs. Allow users to control how a workflow runs, making your workflows more reusable. Learn to import values and deliver the best user experience for your workflows while enhancing accuracy.
- Optimization Strategies in FME Flow: Explore the creation and strategic deployment of parameters in FME Flow, including the use of deployment and geometry parameters, to maximize workflow efficiency.
- Pro Tips for Success: Gain insights on parameterizing connections and leveraging new features like Conditional Visibility for clarity and simplicity.
We’ll wrap up with a glimpse into future webinars, followed by a Q&A session to address your specific questions surrounding this topic.
Don’t miss this opportunity to elevate your FME expertise and drive your projects to new heights of efficiency.
State of ICS and IoT Cyber Threat Landscape Report 2024 previewPrayukth K V
The IoT and OT threat landscape report has been prepared by the Threat Research Team at Sectrio using data from Sectrio, cyber threat intelligence farming facilities spread across over 85 cities around the world. In addition, Sectrio also runs AI-based advanced threat and payload engagement facilities that serve as sinks to attract and engage sophisticated threat actors, and newer malware including new variants and latent threats that are at an earlier stage of development.
The latest edition of the OT/ICS and IoT security Threat Landscape Report 2024 also covers:
State of global ICS asset and network exposure
Sectoral targets and attacks as well as the cost of ransom
Global APT activity, AI usage, actor and tactic profiles, and implications
Rise in volumes of AI-powered cyberattacks
Major cyber events in 2024
Malware and malicious payload trends
Cyberattack types and targets
Vulnerability exploit attempts on CVEs
Attacks on counties – USA
Expansion of bot farms – how, where, and why
In-depth analysis of the cyber threat landscape across North America, South America, Europe, APAC, and the Middle East
Why are attacks on smart factories rising?
Cyber risk predictions
Axis of attacks – Europe
Systemic attacks in the Middle East
Download the full report from here:
https://sectrio.com/resources/ot-threat-landscape-reports/sectrio-releases-ot-ics-and-iot-security-threat-landscape-report-2024/
UiPath Test Automation using UiPath Test Suite series, part 4DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 4. In this session, we will cover Test Manager overview along with SAP heatmap.
The UiPath Test Manager overview with SAP heatmap webinar offers a concise yet comprehensive exploration of the role of a Test Manager within SAP environments, coupled with the utilization of heatmaps for effective testing strategies.
Participants will gain insights into the responsibilities, challenges, and best practices associated with test management in SAP projects. Additionally, the webinar delves into the significance of heatmaps as a visual aid for identifying testing priorities, areas of risk, and resource allocation within SAP landscapes. Through this session, attendees can expect to enhance their understanding of test management principles while learning practical approaches to optimize testing processes in SAP environments using heatmap visualization techniques
What will you get from this session?
1. Insights into SAP testing best practices
2. Heatmap utilization for testing
3. Optimization of testing processes
4. Demo
Topics covered:
Execution from the test manager
Orchestrator execution result
Defect reporting
SAP heatmap example with demo
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
The Art of the Pitch: WordPress Relationships and SalesLaura Byrne
Clients don’t know what they don’t know. What web solutions are right for them? How does WordPress come into the picture? How do you make sure you understand scope and timeline? What do you do if sometime changes?
All these questions and more will be explored as we talk about matching clients’ needs with what your agency offers without pulling teeth or pulling your hair out. Practical tips, and strategies for successful relationship building that leads to closing the deal.
Key Trends Shaping the Future of Infrastructure.pdfCheryl Hung
Keynote at DIGIT West Expo, Glasgow on 29 May 2024.
Cheryl Hung, ochery.com
Sr Director, Infrastructure Ecosystem, Arm.
The key trends across hardware, cloud and open-source; exploring how these areas are likely to mature and develop over the short and long-term, and then considering how organisations can position themselves to adapt and thrive.
Key Trends Shaping the Future of Infrastructure.pdf
Osmotic stress and water isotope effects in kinesin-1 gliding motility assays
1. Osmotic stress and water isotope effects in kinesin-1 gliding motility assays Steve Koch, U. New Mexico Dept. Physics and Center for High Technology Materials (CHTM) Thermodynamics and kinetics of molecular motors workshop Santa Fe, May 20, 2010 Open Notebook Science
2. Important corrections to talk SJK Note, May 28, 2010. After presenting our data in Santa Fe, Erik Schäffer pointed out to us that our slight increase in speed with time was surely due to a slow increase in the temperature of the microscope body due to the arc lamp. This was initially counter-intuitive to me, but I finally understood his point. This week, following the workshop, Andy did a few gliding assays which supported the notion. He and I also did some temperature studies which showed that, indeed, the heating of the microscope continues over the course of many hours. So, at this point we cannot say that there is any speed difference due to the type of surface passivation. Andy’s purchasing components to stabilize the temperature so we can prove this and also eliminate the artifact from future assays. A big thank you to Erik for taking the time to explain this to us and also to Stefan Diez who suggested the same thing to us at the Biophysical Society Annual Meeting, but it didn’t sink in at that point (we thought he meant the photons from arc lamp were causing the problem and we ruled that out). On the following slides, I’ll show our evidence from this week’s studies Speed differences seen in different caseins almost surely an artifact due to increase in microscope temperature over the course of many hours Slow increase in speed for each data set due to the same cause. See also: http://openwetware.org/wiki/User:Andy_Maloney/Notebook/Lab_Notebook_of_Andy_Maloney/2010/05/27/Casein_data_revisited http://friendfeed.com/stevekoch/e439fcd6/microscope-warming-study
3. Correction slide 1 Andy took the casein data all in the same morning. First whole casein, then alpha, then beta. Not sure about Kappa. Andy plotted the data versus absolute time, and they support the notion of a heating effect http://openwetware.org/wiki/User:Andy_Maloney/Notebook/Lab_Notebook_of_Andy_Maloney/2010/05/27/Casein_data_revisited
4. Correction slide 2 Andy ran other experiments earlier this week to support the temperature effects, and the notion that it’s the microscope body, not the photons coming from lamp. A sample made from only coverglass shows same long-time speed increase, Without the initial extra-slow points This is the same kind of sample as on the left, observed after the sample on the left was done. Consistent with microscope continuing to warm. http://openwetware.org/wiki/User:Andy_Maloney/Notebook/Lab_Notebook_of_Andy_Maloney/2010/05/25/More_on_kinesin_speed_effects_due_to_temperature
5. Correction slide 3 We also measured the temperature of our microscope. After cooling overnight, upon turning on the arc lamp, it indeed continues increasing in temperature for many hours. The y-axis is 1 mV = 1 degree F. So, temp increases from about 72 to almost 76 in 5+ hours. Probably still rising. We measured the temperature with a probe in immersion oil on top of the objective, covered by a 1.2 mm thick slide. We’re very sure now that Erik Schaffer was correct. Next week, we’ll confirm by using a temperature-controlled system. See Andy’s notebook for design details http://friendfeed.com/stevekoch/e439fcd6/microscope-warming-study Lamp turned on at 45 mins
6. Acknowledgments Susan Atlas —Lead of the DTRA project UNM Physics / Cancer Center / Director of CARC Haiqing Liu (G. Mantano lab) —Microdevice applications of kinesin LANL & Center for Integrated Nanotechnology (CINT) Collaborations Funding DTRA —DTRA CB Basic Research Program under Grant No. HDTRA1-09-1-008 Larry Herskowitz Physics Ph.D. Student Anthony Salvagno Physics Ph.D. Student Andy Maloney Physics Ph.D. Student Brian Josey Physics B.S. Student Pranav Rathi Optics Ph.D. Student Postdoc training George Bachand— Microdevice applications of kinesin Sandia & Center for Integrated Nanotechnology (CINT)
9. Gliding motility assay Andy Maloney Gliding motility assays Caseins Heavy Water Osmotic Stress 110 microns Assay details Dmk401 30 ug / ml MTs 29% TRITC cytoskeleton bovine Room temp. (See Andy’s poster for more details) Video: Beta casein 6x speed; false-colored Glass surface microtubule kinesin casein
10. Gliding motility assay Andy can finish one sample in 1 hour from start to finish 30 minutes of data, ~9,000 images Primary measurement: speed Also other qualitative measurements Advantage: lots of data quickly; robust assay Glass surface microtubule casein
11. We’ve developed an open-source, automated microtubule tracking and speed analysis platform 1. Automated Tracking of MTs Larry Herskowitz Image tracking Stochastic Simulation Image Simulation MTs identified with NI Vision 7.1 Segmenting Routines Position and Angle of MT ends found via image pattern matching X,Y position versus time recorded for all MTs in folder heirarchy 30 minutes of raw data processed in approx. 1 hour using 4 cores See Larry’s poster today for details Template for pattern matching
12. 2. Automated Speed Analysis A. Track smoothing X, Y data are noisy; dominated by Brownian motion of MT ends Smoothed with Gaussian-weighted 2 second window (microns) (microns)
13. 2. Automated speed analysis B. Instantaneous speed Speed i = |∆r i |/ ∆ t i Alternative to fitting paths to simple shapes Careful for smoothing errors -> What is gliding speed anyway?
14. 2. Automated speed analysis C. Kernel density estimation of speed probability distribution function (PDF) Gaussian kernel, bandwidth 50 nm / s Silverman 1986 Density Estimations
15. 2. Automated speed analysis C. Kernel density estimation of speed probability distribution function (PDF) We checked our algorithm using simulated microtubule image series * constant gliding speed w/ pause of random duration * Herskowitz and Koch (2010) Nature Precedings http://dx.doi.org/10.1038/npre.2010.4374.1
16. 2. Automated speed analysis C. Kernel density estimation of speed probability distribution function (PDF) We checked our algorithm using simulated microtubule image series * constant gliding speed w/ pause of random duration Calculated speed 805.4 +/- 0.2 nm/s (N=15) Actual gliding speed 807 nm/s Pauses probably bias data a bit … more significant with wider bandwidth
17. 2. Automated speed analysis C. Speed PDF analysis not greatly affected by pauses or tracking errors Permits automated speed determination for pooled MT tracks for given condition Most likely speed 670 +/- 5 nm / s N = 46 microtubule tracks Two minutes, 600 images, alpha casein data
18. 2. Automated speed analysis C. Speed PDF analysis not greatly affected by pauses or tracking errors Permits automated speed determination for pooled MT tracks for given condition Alpha casein passivation N=710 MT tracks ~30 minutes assay time Analysis time ~10 minutes on one core See above correction slides. The slow increase in speed is almost surely due to slow Increase in speed of the microscope, due to the arc lamp
19. 2. Automated speed analysis Speed gradually increases with time for all casein varieties Intrinsic speed differences between varieties See also: Verma, V., Hancock, W. O., & Catchmark, J. M. (2008). Journal of biological engineering , 2 , 14. doi: 10.1186/1754-1611-2-14. See above correction slides. These speed differences and the slow increase in speed with time are almost surely an artifact due to slowly increasing temperature of the microscope due to the arc lamp
20. Varying the properties of water may be a useful knob for studying kinesin Water isotope effects Heavy-hydrogen water (D 2 O) Heavy-oxygen water (H 2 18 O) Osmotic stress Betaine, Future work: other osmolytes: proline, sucrose, etc. Future: denaturants May be a method for probing large-surface area interactions between kinesin and tubulin
21. Osmotic stress dramatically increases lifetime of bound molecular complexes An example from protein-DNA interactions Off rate reduced by >100 fold ln(Fraction bound) Sidorova and Rau No kinesin studies yet; A few myosin studies
22. Heavy-hydrogen water (D2O) has been used to probe the actomyosin system Chaen, S., et al (2001). Effect of deuterium oxide on actomyosin motility in vitro. Biochimica et biophysica acta , 1506 (3), 218-23. Pub med: 11779555. The authors list among many possible explanations, an effect on myosin-actin affinity. How should water isotopes affect kinesin motility? Answers from molecular dynamics?
23. Another reason we’re looking at water effects is our partnership with Atlas / Valone labs Susan Atlas (UNM; PI of our DTRA project) and Steve Valone (LANL) are developing the “Charge-Transfer Embedded-Atom Model (CT-EAM)” force field for MD simulations of kinesin and other biomolecules. Incorporates “correct” quantum mechanics so that charge transfer can be handled. One of the first benefits of CT-EAM will be better simulation of water dynamics…including for differing isotopes. Big disconnect between length and time scales for gliding assay and MD But both can look at water dynamics. MD: How many hydrating water molecules? How do water dynamics change with isotope?
24. Stochastic simulation can help us understand how water might affect kinesin motility Larry Herskowitz has developed an agent-based stochastic simulation to help us interpret and guide experiments more info at his poster We can ask question: “What will happen if Kinesin-MT off rate decreases?” The answers aren’t always obvious when pathway can branch.
25. Stochastic simulation can help us understand how water might affect kinesin motility Stochastic simulations tentatively indicate that increased osmotic pressure or increased water mass would decrease kinesin speed.
26. Heavy-hydrogen water reduces MT gliding speed. As with regular water, speed gradually increases with time Mean and SEM for last 8 points of each set. See above correction slides. The slow increase in speed is almost surely due to slow Increase in speed of the microscope, due to the arc lamp. The differences between isotopes is real, and potentially differences are greater than we see.
27. Heavy-hydrogen water reduces MT gliding speed. Speed steadily decreases as deuterium conc. Increases. Also, noticed other features (preliminarily): Reduced photobleaching Longer-lasting MTs MT-MT sticking
28. Other features seen in D2O assays Also, noticed other features (preliminarily): Reduced photobleaching Longer-lasting MTs MT-MT sticking It turns out there is existing literature to support all of these observations
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30. Heavy-oxygen water See above correction slides. The slow increase in speed is almost surely due to slow Increase in speed of the microscope, due to the arc lamp. The differences between isotopes is real, and potentially differences are greater than we see.
31. Surprisingly, heavy-oxygen water has similar effect on gliding speed Chemistry of oxygen-18 water similar to regular water Viscosity of oxygen-18 water only 5% higher than regular water (23% for D2O) * . Heavy-O water much less confusing, possibly more tractable theoretically. Expensive, though ($1,000 / gram) Possible that Heavy-O water is a good probe of kinesin-MT surface interaction * Viscosity: Kudish et al. J. Chem. Soc., Faraday Trans. 1 , 1972, 68 , 2041 - 2046, DOI: 10.1039/F19726802041
32. Betaine (osmolyte) substantially reduces gliding speed Unsure how important viscosity increase is. Need to repeat with other osmolytes See above correction slides. The slow increase in speed is almost surely due to slow Increase in speed of the microscope, due to the arc lamp. The differences between osmolyte concentrations is real.
33. Current conclusions from water and other experiments Heavy-oxygen water looks like promising probe of hydration effects Heavy-hydrogen water may also be interesting probe, but more complicated. May have benefits for stabilization of protein in the lab or in microdevices. Betaine has strong effect on gliding speed. Viscosity a confounding variable. Need to compare with other osmolytes. Need a better grasp of solution thermodynamics and kinetics and effect on motor proteins. Type of surface passivation affects gliding speed. Gliding speed also increases with time.
34. Open Data, Open-source Software All data presented here is CC0-licensed and available online: http://openwetware.org/wiki/Koch_Lab:Data/MT_Gliding_Assay_Readme_File We’re still working on the organization and how to best share. In the meantime: If useful to you, get in touch and we can help you with the software and / or figuring out the data sets!
35. Acknowledgments Susan Atlas —Lead of the DTRA project UNM Physics / Cancer Center / Director of CARC Haiqing Liu (G. Mantano lab) —Microdevice applications of kinesin LANL & Center for Integrated Nanotechnology (CINT) Collaborations Funding DTRA —DTRA CB Basic Research Program under Grant No. HDTRA1-09-1-008 Larry Herskowitz Physics Ph.D. Student Anthony Salvagno Physics Ph.D. Student Andy Maloney Physics Ph.D. Student Brian Josey Physics B.S. Student Pranav Rathi Optics Ph.D. Student Postdoc training George Bachand— Microdevice applications of kinesin Sandia & Center for Integrated Nanotechnology (CINT)