The document provides an overview of an experimental project studying the motor protein kinesin using various single molecule techniques like optical tweezers and magnetic tweezers. The project is investigating the effects of heavy water (D2O) on kinesin-driven microtubule motility using gliding motility assays. Preliminary results show that microtubules are more stable in D2O, remaining active for over 24 hours compared to regular assays. Velocity measurements of microtubule movement are also being conducted. Future work will explore isotope effects using 18-oxygen water and the effects of osmotic stress on motility. The project involves collaborations between the documenting lab and other groups studying modeling and applications.
Studying Retinal Function in Large Animals: Laser-Induced Choroidal Neovascul...InsideScientific
In this webinar, sponsored by Phoenix Research Labs, Dr. Christine van Hover introduces the Micron X large animal retinal imaging systems, designed specifically for the challenges of large animal eye and eye-brain research. Dr. van Hover discusses how to take high quality images of the whole large animal retina, what species can be imaged with the Micron X camera and shows examples of fluorescein angiography in non-human primates.
Dr. David Culp, Director of Research at Powered Research, presents data from a recent study designed to evaluate and characterize a porcine model of laser induced choroidal neovascularization (CNV). CNV complexes were created in weanling pigs, followed by intravitreal injections of aflibercept or balanced salt saline. Dr. Culp provides specific examples of his data where fundus photography, fluorescein angiography (FA), and retinal optical coherence tomography (OCT) were performed. He will also shows how the Micron X large animal imaging system can produce high resolution fluorescent images to quantify neovascularization. Viewers of this webinar will gain an understanding of how to setup and employ a large animal imaging system in their own laboratory.
Investigating cellular metabolism with the 3D Cell ExplorerMathieuFRECHIN
Long-term imaging of fine dynamics of cellular organelles is today’s biggest challenge in cell biology
(Frechin et al., 2015; Kruse & Jülicher, 2005; Kueh, Champhekhar, Nutt, Elowitz, & Rothenberg, 2013;
Skylaki, Hilsenbeck, & Schroeder, 2016). The goal is to acquire not only snapshots of dynamic biological
systems, but to actually see processes unfolding over time in term of spatial and morphological
changes and biological outcome (Muzzey, Gómez-Uribe, Mettetal, & van Oudenaarden, 2009).
Imaging over time is of utmost importance in the study of key organelles implicated in cellular
metabolism: mitochondria and lipid droplets. The current method of choice in high-content live
imaging approaches is fluorescence microscopy. However, fluorescence microscopy induces
phototoxicity when the sample is stimulated at various wavelengths. This stress induces cellular
damages via radical-induced cellular structure alterations, which limits live imaging possibilities.
Therefore, with the current live cell imaging strategies a tradeoff must be found between short
live cell imaging with high-frequency acquisition or long-term live cell imaging with low-frequency
acquisition.
On one hand, high-frequency acquisition induces a lot of phototoxic stress and, if successful, a
researcher might observe fine dynamics but cannot be sure that they have not been perturbed
by the imaging process. On the other hand, low-frequency acquisition might be more sustainable,
however, fine dynamics are lost, while the observed phenomenon, to a lesser extent, could likewise
be perturbed by the imaging process.
Studying Retinal Function in Large Animals: Laser-Induced Choroidal Neovascul...InsideScientific
In this webinar, sponsored by Phoenix Research Labs, Dr. Christine van Hover introduces the Micron X large animal retinal imaging systems, designed specifically for the challenges of large animal eye and eye-brain research. Dr. van Hover discusses how to take high quality images of the whole large animal retina, what species can be imaged with the Micron X camera and shows examples of fluorescein angiography in non-human primates.
Dr. David Culp, Director of Research at Powered Research, presents data from a recent study designed to evaluate and characterize a porcine model of laser induced choroidal neovascularization (CNV). CNV complexes were created in weanling pigs, followed by intravitreal injections of aflibercept or balanced salt saline. Dr. Culp provides specific examples of his data where fundus photography, fluorescein angiography (FA), and retinal optical coherence tomography (OCT) were performed. He will also shows how the Micron X large animal imaging system can produce high resolution fluorescent images to quantify neovascularization. Viewers of this webinar will gain an understanding of how to setup and employ a large animal imaging system in their own laboratory.
Investigating cellular metabolism with the 3D Cell ExplorerMathieuFRECHIN
Long-term imaging of fine dynamics of cellular organelles is today’s biggest challenge in cell biology
(Frechin et al., 2015; Kruse & Jülicher, 2005; Kueh, Champhekhar, Nutt, Elowitz, & Rothenberg, 2013;
Skylaki, Hilsenbeck, & Schroeder, 2016). The goal is to acquire not only snapshots of dynamic biological
systems, but to actually see processes unfolding over time in term of spatial and morphological
changes and biological outcome (Muzzey, Gómez-Uribe, Mettetal, & van Oudenaarden, 2009).
Imaging over time is of utmost importance in the study of key organelles implicated in cellular
metabolism: mitochondria and lipid droplets. The current method of choice in high-content live
imaging approaches is fluorescence microscopy. However, fluorescence microscopy induces
phototoxicity when the sample is stimulated at various wavelengths. This stress induces cellular
damages via radical-induced cellular structure alterations, which limits live imaging possibilities.
Therefore, with the current live cell imaging strategies a tradeoff must be found between short
live cell imaging with high-frequency acquisition or long-term live cell imaging with low-frequency
acquisition.
On one hand, high-frequency acquisition induces a lot of phototoxic stress and, if successful, a
researcher might observe fine dynamics but cannot be sure that they have not been perturbed
by the imaging process. On the other hand, low-frequency acquisition might be more sustainable,
however, fine dynamics are lost, while the observed phenomenon, to a lesser extent, could likewise
be perturbed by the imaging process.
2009 September Kinesin Talk at UNM ChemistrySteve Koch
Talk given by Steve at the Unviversity of New Mexico Chemistry Department on September 11, 2009. It is mostly still an introduction to our kinesin project, but now I'm able to include the latest results from gliding motility assay, tracking software, and stochastic kinetics simulation.
Osmotic stress and water isotope effects in kinesin-1 gliding motility assaysSteve Koch
The osmotic pressure and kinetic properties of water play important roles in biomolecular interactions. As pointed out by Parsegian, Rand, and Rau, these crucial roles are often overlooked1. In some fields, osmotic stress and isotope effects have been exploited for probing the role water plays in binding interactions of biomolecules. To our knowledge, there have been no studies of osmotic stress and water isotope effects for kinesin, and only a handful for myosin. We’re currently using the gliding motility assay to see whether we can extract new information about kinesin-1 / microtubule interactions by changing osmotic stress and water isotopes. We will describe our open-source, automated analysis platform for extracting microtubule gliding speeds from image series. We will also show our preliminary analyses of the changes seen in gliding assays when done in heavy water (either heavy-hydrogen or heavy-oxygen) or osmolytes (betaine). We will discuss whether osmotic stress and isotopes, particularly heavy-oxygen water, might be an important tool for probing effects of water on binding interactions between kinesin and microtubules. We will also discuss potential applications of deuterium water for stabilizing microtubules and kinesin for lab or device applications.
[1] Parsegian, V. A., Rand, R. P., & Rau, D. C. (1995). Macromolecules and water: probing with osmotic stress. Methods in Enzymology, 259.
This work was supported by the DTRA CB Basic Research Program under Grant No. HDTRA1-09-1-008 in collaboration with Dr. Susan Atlas lab (UNM).
Computational Biophysics in the Petascale Computing Erainside-BigData.com
In this deck from the Blue Waters Symposium, Dr. Rommie E. Amaro from UC San Diego presents: Computational Biophysics in the Petascale Computing Era.
"Advances in structural, chemical, and biophysical data acquisition (e.g., protein structures via X-ray crystallography and near atomic cryoEM, isothermal calorimetry, etc.), coupled with the continued exponential growth in computing power and advances in the underlying algorithms are opening a new era for the simulation of biological systems at the molecular level, and at scales never before reached. In this talk I will discuss how the BlueWaters Petascale computing architecture forever altered the landscape and potential of computational biophysics. In particular, new and emerging capabilities for multiscale dynamic simulations that cross spatial scales from the molecular (angstrom) to cellular ultrastructure (near micron), and temporal scales from the picoseconds of macromolecular dynamics to the physiologically important time scales of organelles and cells (milliseconds to seconds) are now possible. These efforts are driven by the outstanding and persistent advances in peta- and exascale computing and availability of multimodal biological datasets, as well as by gaps in current abilities to connect across scales where it is already clear that new approaches will result in novel fundamental understanding of biological phenomena or open new therapeutic avenues."
Watch the video: https://wp.me/p3RLHQ-j1u
Learn more: https://bluewaters.ncsa.illinois.edu/blue-waters-symposium-2018
Sign up for our insideHPC Newsletter: http://insidehpc.com/newsletter
The Algorithms of Life - Scientific Computing for Systems Biologyinside-BigData.com
In this deck from ISC 2019, Ivo Sbalzarini from TU Dresden presents: The Algorithms of Life - Scientific Computing for Systems Biology. In his talk, Sbalzarini mainly discussed the rapidly growing importance and influence in the life sciences for scientific high-performance computing.
"Scientific high-performance computing is of rapidly growing importance and influence in the life sciences. Thanks to the increasing knowledge about the molecular foundations of life, recent advances in biomedical data science, and the availability of predictive biophysical theories that can be numerically simulated, mechanistic understanding of the emergence of life comes within reach. Computing is playing a pivotal and catalytic role in this scientific revolution, both as a tool of investigation and hypothesis testing, but also as a school of thought and systems model. This is because a developing tissue, embryo, or organ can itself be seen as a massively parallel distributed computing system that collectively self-organizes to bring about behavior we call life. In any multicellular organism, every cell constantly takes decisions about growth, division, and migration based on local information, with cells communicating with each other via chemical, mechanical, and electrical signals across length scales from nanometers to meters. Each cell can therefore be understood as a mechano-chemical processing element in a complexly interconnected million- or billion-core computing system. Mechanistically understanding and reprogramming this system is a grand challenge. While the “hardware” (proteins, lipids, etc.) and the “source code” (genetic code) are increasingly known, we known virtually nothing about the algorithms that this code implements on this hardware. Our vision is to contribute to this challenge by developing computational methods and software systems for high-performance data analysis, inference, and numerical simulation of computer models of biological tissues, incorporating the known biochemistry and biophysics in 3D-space and time, in order to understand biological processes on an algorithmic basis. This ranges from real-time approaches to biomedical image analysis, to novel simulation languages for parallel high-performance computing, to virtual reality and machine learning for 3D microscopy and numerical simulations of coupled biochemical-biomechanical models. The cooperative, interdisciplinary effort to develop and advance our understanding of life using computational approaches not only places high-performance computing center stage, but also provides stimulating impulses for the future development of this field."
Watch the video: https://wp.me/p3RLHQ-kBB
Learn more: https://www.isc-hpc.com/
Sign up for our insideHPC Newsletter: http://insidehpc.com/newsletter
ATEAS V1(2):: American Transactions on Engineering & Applied Sciencesdrboon
Research from American Transactions on Engineering & Applied Sciences:: A Detailed Analysis of Capillary Viscometer
Fuzzy Logic Modeling Approach for Risk Area Assessment for Hazardous Materials Transportation
Computer Modeling of Internal Pressure Autofrettage Process of a Thick-Walled Cylinder with the Bauschinger Effect
Types of Media for Seeds Germination and Effect of BA on Mass Propagation of Nepenthes mirabilis Druce
Numerical Analysis of Turbulent Diffusion Combustion in Porous Media
Production of Hydrocarbons from Palm Oil over NiMo Catalyst
Philosophy of Biological Cell Repair informs Geoethical Nanotechnology: Cellular repair is an age-old function in biology. This talk examines the cellular process of repair in philosophical terms. Biologically, wound-healing is the primary form of cellular repair, drawing on numerous cell types and the extracellular matrix to perform a variety of operations during the phases of inflammation, proliferation, and maturation. Philosophically, these functions can be discussed from a systems theory perspective, through the concepts pairs of parts-whole, autonomy-dependency, self-other, sickness-wellness, and scarcity-abundance. Understanding cellular repair at the theory level could facilitate the development of nanotechnology solutions that augment biological processes in ways that are congruently geoethical with nature’s ethos.
DNA sequencing: rapid improvements and their implicationsJeffrey Funk
these slides analyze the rapid improvements in DNA sequencers and the implications for these rapid improvements for drug discovery, new crops, materials creation, and new bio-fuels. Many of the rapid improvements are from "reductions in scale." As with integrated circuits, reducing the size of features on DNA sequencers has enabled many orders of magnitude improvements in them. Unlike integrated circuits, the improvements are also due to changes in technology. For example, changes from pyrosequencing to semiconductor and nanopore sequencing have also been needed to achieve the reductions in scale. Second, pyrosequencing also benefited from improvements in lasers and camera chips.
2011 NSF CAREER_Steve Koch Full Project Description Steve Koch
This is the full Project Description for my 2011 NSF CAREER proposal. As I described on my blog, I am disappointed in the unfinished product, mostly because I still think the proposed research is important, exciting, and achievable by my lab. ( http://stevekochresearch.blogspot.com/2011/08/2011-nsf-career-proposal-ugh-failures.html )
Here are links to prior years' proposals, which were declined:
* 2009 http://www.scribd.com/doc/17548381/2009-ProposalCAREER-SingleMolecule-Analysis-of-Genomic-DNA-and-Chromatin-in-Eukaryotic-Transcription
* 2008 http://www.scribd.com/doc/10196076/2008-NSF-CAREERproposal-Only
2009 September Kinesin Talk at UNM ChemistrySteve Koch
Talk given by Steve at the Unviversity of New Mexico Chemistry Department on September 11, 2009. It is mostly still an introduction to our kinesin project, but now I'm able to include the latest results from gliding motility assay, tracking software, and stochastic kinetics simulation.
Osmotic stress and water isotope effects in kinesin-1 gliding motility assaysSteve Koch
The osmotic pressure and kinetic properties of water play important roles in biomolecular interactions. As pointed out by Parsegian, Rand, and Rau, these crucial roles are often overlooked1. In some fields, osmotic stress and isotope effects have been exploited for probing the role water plays in binding interactions of biomolecules. To our knowledge, there have been no studies of osmotic stress and water isotope effects for kinesin, and only a handful for myosin. We’re currently using the gliding motility assay to see whether we can extract new information about kinesin-1 / microtubule interactions by changing osmotic stress and water isotopes. We will describe our open-source, automated analysis platform for extracting microtubule gliding speeds from image series. We will also show our preliminary analyses of the changes seen in gliding assays when done in heavy water (either heavy-hydrogen or heavy-oxygen) or osmolytes (betaine). We will discuss whether osmotic stress and isotopes, particularly heavy-oxygen water, might be an important tool for probing effects of water on binding interactions between kinesin and microtubules. We will also discuss potential applications of deuterium water for stabilizing microtubules and kinesin for lab or device applications.
[1] Parsegian, V. A., Rand, R. P., & Rau, D. C. (1995). Macromolecules and water: probing with osmotic stress. Methods in Enzymology, 259.
This work was supported by the DTRA CB Basic Research Program under Grant No. HDTRA1-09-1-008 in collaboration with Dr. Susan Atlas lab (UNM).
Computational Biophysics in the Petascale Computing Erainside-BigData.com
In this deck from the Blue Waters Symposium, Dr. Rommie E. Amaro from UC San Diego presents: Computational Biophysics in the Petascale Computing Era.
"Advances in structural, chemical, and biophysical data acquisition (e.g., protein structures via X-ray crystallography and near atomic cryoEM, isothermal calorimetry, etc.), coupled with the continued exponential growth in computing power and advances in the underlying algorithms are opening a new era for the simulation of biological systems at the molecular level, and at scales never before reached. In this talk I will discuss how the BlueWaters Petascale computing architecture forever altered the landscape and potential of computational biophysics. In particular, new and emerging capabilities for multiscale dynamic simulations that cross spatial scales from the molecular (angstrom) to cellular ultrastructure (near micron), and temporal scales from the picoseconds of macromolecular dynamics to the physiologically important time scales of organelles and cells (milliseconds to seconds) are now possible. These efforts are driven by the outstanding and persistent advances in peta- and exascale computing and availability of multimodal biological datasets, as well as by gaps in current abilities to connect across scales where it is already clear that new approaches will result in novel fundamental understanding of biological phenomena or open new therapeutic avenues."
Watch the video: https://wp.me/p3RLHQ-j1u
Learn more: https://bluewaters.ncsa.illinois.edu/blue-waters-symposium-2018
Sign up for our insideHPC Newsletter: http://insidehpc.com/newsletter
The Algorithms of Life - Scientific Computing for Systems Biologyinside-BigData.com
In this deck from ISC 2019, Ivo Sbalzarini from TU Dresden presents: The Algorithms of Life - Scientific Computing for Systems Biology. In his talk, Sbalzarini mainly discussed the rapidly growing importance and influence in the life sciences for scientific high-performance computing.
"Scientific high-performance computing is of rapidly growing importance and influence in the life sciences. Thanks to the increasing knowledge about the molecular foundations of life, recent advances in biomedical data science, and the availability of predictive biophysical theories that can be numerically simulated, mechanistic understanding of the emergence of life comes within reach. Computing is playing a pivotal and catalytic role in this scientific revolution, both as a tool of investigation and hypothesis testing, but also as a school of thought and systems model. This is because a developing tissue, embryo, or organ can itself be seen as a massively parallel distributed computing system that collectively self-organizes to bring about behavior we call life. In any multicellular organism, every cell constantly takes decisions about growth, division, and migration based on local information, with cells communicating with each other via chemical, mechanical, and electrical signals across length scales from nanometers to meters. Each cell can therefore be understood as a mechano-chemical processing element in a complexly interconnected million- or billion-core computing system. Mechanistically understanding and reprogramming this system is a grand challenge. While the “hardware” (proteins, lipids, etc.) and the “source code” (genetic code) are increasingly known, we known virtually nothing about the algorithms that this code implements on this hardware. Our vision is to contribute to this challenge by developing computational methods and software systems for high-performance data analysis, inference, and numerical simulation of computer models of biological tissues, incorporating the known biochemistry and biophysics in 3D-space and time, in order to understand biological processes on an algorithmic basis. This ranges from real-time approaches to biomedical image analysis, to novel simulation languages for parallel high-performance computing, to virtual reality and machine learning for 3D microscopy and numerical simulations of coupled biochemical-biomechanical models. The cooperative, interdisciplinary effort to develop and advance our understanding of life using computational approaches not only places high-performance computing center stage, but also provides stimulating impulses for the future development of this field."
Watch the video: https://wp.me/p3RLHQ-kBB
Learn more: https://www.isc-hpc.com/
Sign up for our insideHPC Newsletter: http://insidehpc.com/newsletter
ATEAS V1(2):: American Transactions on Engineering & Applied Sciencesdrboon
Research from American Transactions on Engineering & Applied Sciences:: A Detailed Analysis of Capillary Viscometer
Fuzzy Logic Modeling Approach for Risk Area Assessment for Hazardous Materials Transportation
Computer Modeling of Internal Pressure Autofrettage Process of a Thick-Walled Cylinder with the Bauschinger Effect
Types of Media for Seeds Germination and Effect of BA on Mass Propagation of Nepenthes mirabilis Druce
Numerical Analysis of Turbulent Diffusion Combustion in Porous Media
Production of Hydrocarbons from Palm Oil over NiMo Catalyst
Philosophy of Biological Cell Repair informs Geoethical Nanotechnology: Cellular repair is an age-old function in biology. This talk examines the cellular process of repair in philosophical terms. Biologically, wound-healing is the primary form of cellular repair, drawing on numerous cell types and the extracellular matrix to perform a variety of operations during the phases of inflammation, proliferation, and maturation. Philosophically, these functions can be discussed from a systems theory perspective, through the concepts pairs of parts-whole, autonomy-dependency, self-other, sickness-wellness, and scarcity-abundance. Understanding cellular repair at the theory level could facilitate the development of nanotechnology solutions that augment biological processes in ways that are congruently geoethical with nature’s ethos.
DNA sequencing: rapid improvements and their implicationsJeffrey Funk
these slides analyze the rapid improvements in DNA sequencers and the implications for these rapid improvements for drug discovery, new crops, materials creation, and new bio-fuels. Many of the rapid improvements are from "reductions in scale." As with integrated circuits, reducing the size of features on DNA sequencers has enabled many orders of magnitude improvements in them. Unlike integrated circuits, the improvements are also due to changes in technology. For example, changes from pyrosequencing to semiconductor and nanopore sequencing have also been needed to achieve the reductions in scale. Second, pyrosequencing also benefited from improvements in lasers and camera chips.
2011 NSF CAREER_Steve Koch Full Project Description Steve Koch
This is the full Project Description for my 2011 NSF CAREER proposal. As I described on my blog, I am disappointed in the unfinished product, mostly because I still think the proposed research is important, exciting, and achievable by my lab. ( http://stevekochresearch.blogspot.com/2011/08/2011-nsf-career-proposal-ugh-failures.html )
Here are links to prior years' proposals, which were declined:
* 2009 http://www.scribd.com/doc/17548381/2009-ProposalCAREER-SingleMolecule-Analysis-of-Genomic-DNA-and-Chromatin-in-Eukaryotic-Transcription
* 2008 http://www.scribd.com/doc/10196076/2008-NSF-CAREERproposal-Only
This is a summary I gave at group meeting today on what I'd learned about D2O (aka "heavy water" aka "deuterium oxide") and its effects on biochemistry/biophysics of enzymes and proteins.
Here are the slides describing talents and hedgehog concepts in the context of students' future careers. It's the background to our final assignment for the semester: http://openwetware.org/wiki/User:Steven_J._Koch/Talents_assignment
Discussion of dispersion and rainbows. Also some cool photos of blackbody and fluorescent spectra from Tom Decaro and Analisa Goodman as part of the homework question.
08 Feb 17 Light, Electron E Levels Actual PresentedSteve Koch
Introduction to electromagnetic radiation and light. Viewing atomic spectra with diffraction gratings. Optical tweezers (cool example of light having momentum).
This is lecture 5, Wave interference, standing waves, resonance, intro to sound waves. For Conceptual Physics course, Physics 102, at University of New Mexico. Koch's section.
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
UiPath Test Automation using UiPath Test Suite series, part 3DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 3. In this session, we will cover desktop automation along with UI automation.
Topics covered:
UI automation Introduction,
UI automation Sample
Desktop automation flow
Pradeep Chinnala, Senior Consultant Automation Developer @WonderBotz and UiPath MVP
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Key Trends Shaping the Future of Infrastructure.pdfCheryl Hung
Keynote at DIGIT West Expo, Glasgow on 29 May 2024.
Cheryl Hung, ochery.com
Sr Director, Infrastructure Ecosystem, Arm.
The key trends across hardware, cloud and open-source; exploring how these areas are likely to mature and develop over the short and long-term, and then considering how organisations can position themselves to adapt and thrive.
Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But there’s more:
In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
But—if the “Reject” button is pushed, colleagues will be alerted via Slack message
Join us to learn more about this new, human-in-the-loop capability, brought to you by Integration Service connectors.
And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
Builder.ai Founder Sachin Dev Duggal's Strategic Approach to Create an Innova...Ramesh Iyer
In today's fast-changing business world, Companies that adapt and embrace new ideas often need help to keep up with the competition. However, fostering a culture of innovation takes much work. It takes vision, leadership and willingness to take risks in the right proportion. Sachin Dev Duggal, co-founder of Builder.ai, has perfected the art of this balance, creating a company culture where creativity and growth are nurtured at each stage.
UiPath Test Automation using UiPath Test Suite series, part 4DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 4. In this session, we will cover Test Manager overview along with SAP heatmap.
The UiPath Test Manager overview with SAP heatmap webinar offers a concise yet comprehensive exploration of the role of a Test Manager within SAP environments, coupled with the utilization of heatmaps for effective testing strategies.
Participants will gain insights into the responsibilities, challenges, and best practices associated with test management in SAP projects. Additionally, the webinar delves into the significance of heatmaps as a visual aid for identifying testing priorities, areas of risk, and resource allocation within SAP landscapes. Through this session, attendees can expect to enhance their understanding of test management principles while learning practical approaches to optimize testing processes in SAP environments using heatmap visualization techniques
What will you get from this session?
1. Insights into SAP testing best practices
2. Heatmap utilization for testing
3. Optimization of testing processes
4. Demo
Topics covered:
Execution from the test manager
Orchestrator execution result
Defect reporting
SAP heatmap example with demo
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
LF Energy Webinar: Electrical Grid Modelling and Simulation Through PowSyBl -...DanBrown980551
Do you want to learn how to model and simulate an electrical network from scratch in under an hour?
Then welcome to this PowSyBl workshop, hosted by Rte, the French Transmission System Operator (TSO)!
During the webinar, you will discover the PowSyBl ecosystem as well as handle and study an electrical network through an interactive Python notebook.
PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
- A fully editable and extendable library for grid component modelling;
- Visualization tools to display your network;
- Grid simulation tools, such as power flows, security analyses (with or without remedial actions) and sensitivity analyses;
The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
What you will learn during the webinar:
- For beginners: discover PowSyBl's functionalities through a quick general presentation and the notebook, without needing any expert coding skills;
- For advanced developers: master the skills to efficiently apply PowSyBl functionalities to your real-world scenarios.
Smart TV Buyer Insights Survey 2024 by 91mobiles.pdf91mobiles
91mobiles recently conducted a Smart TV Buyer Insights Survey in which we asked over 3,000 respondents about the TV they own, aspects they look at on a new TV, and their TV buying preferences.
Software Delivery At the Speed of AI: Inflectra Invests In AI-Powered QualityInflectra
In this insightful webinar, Inflectra explores how artificial intelligence (AI) is transforming software development and testing. Discover how AI-powered tools are revolutionizing every stage of the software development lifecycle (SDLC), from design and prototyping to testing, deployment, and monitoring.
Learn about:
• The Future of Testing: How AI is shifting testing towards verification, analysis, and higher-level skills, while reducing repetitive tasks.
• Test Automation: How AI-powered test case generation, optimization, and self-healing tests are making testing more efficient and effective.
• Visual Testing: Explore the emerging capabilities of AI in visual testing and how it's set to revolutionize UI verification.
• Inflectra's AI Solutions: See demonstrations of Inflectra's cutting-edge AI tools like the ChatGPT plugin and Azure Open AI platform, designed to streamline your testing process.
Whether you're a developer, tester, or QA professional, this webinar will give you valuable insights into how AI is shaping the future of software delivery.
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
JMeter webinar - integration with InfluxDB and Grafana
2010 DTRA Update
1. Experimental overview of DTRA kinesin projectKoch Lab, UNM Dept. Physics and Center for High Technology Materials (CHTM) “Kiney” Steve Koch, DTRA Co-PI, Experimental LeadAsst. Prof. Physics and Astronomy Larry HerskowitzPhysics Ph.D. Student Andy Maloney Physics Ph.D. Student Anthony Salvagno, IGERT FellowPhysics Ph.D. Student Brian Josey Physics B.S. Student SJK Note: For most of the embedded movies, try thispublic directory: http://kochlab.org/files/Movies/2010%20Feb%20DTRA%20Presentation Email: sjkoch@unm.edu Emmalee Jones, (rotating)NSMS Ph.D. Student
2. KochLab Overview / Acknowledgments Single-molecule manipulation Optical tweezers; magnetic tweezers; MEMS Kinesin / mictrotubules Osmotic stress; isotope effects Protein-DNA interactions; transcription Collaborations Susan Atlas—Lead of the DTRA projectUNM Physics / Cancer Center / Director of CARC HaiqingLiu (G. Mantano lab)—Microdeviceapplications of kinesin LANL & Center for Integrated Nanotechnology (CINT) Evan Evans Lab—Single-molecule thermodynamics and kinetics U. New Mexico / U. British Columbia / Boston U. Funding DTRA—Basic Science; CHTM—Startup; ACS—Jan Oliver IRG
3. Microtubules are polymers of tubulin heterodimers Fast polymerizing end Plus end 8 nanometers 25 nanometers Slow polymerizing end Minus end Tubulin can be purified from, for example, cow brains Microtubules can be reliably polymerized in vitro Stabilized with anti-cancer drug Taxol
4. Our goal is to gain atomistic insight through a variety of experiments and simulations…especially focusing on water Susan Atlas (PI) and Steve Valone (LANL) “Charge transfer embedded atom model” (CT-EAM) Atomistic modeling of kinesin catalytic core Molecular dynamics Kochlab: Biophysical experimental studies of kinesin
5. Gliding motility assay Buffer includes ATP, antifade cocktail Andy is currently leading the GMA project 100 microns Passivated glass surface (casein) Parameters we can measure Speedspeed distribution MT morphology (straight; circles; length) Assay longevity (activity; photobleaching) (Show movie externally) “motility in regular water”
6. Gliding motility assay is initially our main assay Buffer includes ATP, antifade cocktail Passivated glass surface (casein) Operate in the high motor density regime Main experimental result is transport velocity Heavy water Osmotic stress Temperature, metal ions, ATP concentration Site-directed mutagenesis Fascinating early results! Experimental “knobs” to obtain datathat can be compared with theory in the iterative loop
7. Heavy water background Naturally abundant 1 / 6600 hydrogen molecules is deuterium 17 mM deuterium in “standard mean ocean water” 11% denser than H2O. Freezes at 3.8C. D-bonds stronger. Toxic to eukaryotes. The toxic effects are similar to chemotherapeutic drugs. D2O has been used to stabilize viral vaccines. D2O stabilizes tubulin and microtubules. D2O stimulates tubulin assembly formation. (Other fascinating factoids…) Effects on kinesin motility has not yet been studied
8. Gliding assays in D2OSquiggly Microtubules; MT-MT interactions (Show movie externally) “Motility in 100% D2O”
9. Gliding assays in D2OSignificantly more stable microtubules (and maybe kinesin) Activity lasts > 24 hours (Show movie externally) (“motility after 1 day in D2O”) Also reduces photobleaching and possibly the “opticution” effects.
10. Microtubule velocity in gliding assay is measured viaLabVIEW image tracking software written by Larry Open source software Preparing publication (Show movie externally)
11. Gliding motility assay—Deuterium Isotope Effects 7. Guydosh, Nicholas R, and Steven M Block. “Direct observation of the binding state of the kinesin head to the microtubule.” Nature 461, no. 7260 (September 3, 2009): 125-128. doi:10.1038/nature08259. Supplemental information. Preparing publication
12. Implications of DTRA research so far Basic Research Gaining insights into fundamental mechanochemistry of kinesin/MTs Properties of water ideal connection with theory group (didn’t show): Have built stochastic simulation to interpret data Applications D2O Results point towards strategies for improving device robustness Fundamental understanding will guide directed engineering of motors Open source software will help community
13. Next steps – Study isotope effect; osmotic stress 18-oxygen water (does not exchange with protein groups; hydrogen bonding same) Osmolytes (e.g. betaine, sucrose) Is this a real effect?
14. Acknowledgments Our Lab—Larry Herskowitz, Andy Maloney, Anthony Salvagno,Brian Josey, Emmalee Jones, Linh Le, Brigette Black, Igor Kuznetsov Collaborations Susan Atlas—Lead of the DTRA projectUNM Physics / Cancer Center / Director of CARC Steve Valone—Co-PI (LANL) Haiqing Liu—Microdevice applications of kinesin LANL & Center for Integrated Nanotechnology (CINT) Evan Evans Lab—Single-molecule thermodynamics and kinetics U. New Mexico / U. British Columbia / Boston U. Funding DTRA—Basic Science; CHTM—Startup; ACS—Jan Oliver IRG