One Health Capacity Building Workshop was held from March 19-21, 2014 in Yogyakarta, Indonesia. The workshop aimed to promote the One Health approach through training programs by describing its basic principles, participatory learning processes, and designing training programs. It also sought to broaden understanding of emerging zoonotic diseases and pandemic threats among One Health Cadres and future leaders through discussions of key elements of One Health approaches and their application. The workshop was attended by university staff from One Health member institutions as well as other universities in Yogyakarta, Central Java, East Java, and Bali regions.