Cancer chemoprevention with dietary phytochemicalsdegarden
Young-Joon Surh
Chemoprevention refers to the use of agents to inhibit, reverse or retard tumorigenesis.
Numerous phytochemicals derived from edible plants have been reported to interfere with a
specific stage of the carcinogenic process. Many mechanisms have been shown to account
for the anticarcinogenic actions of dietary constituents, but attention has recently been
focused on intracellular-signalling cascades as common molecular targets for various
chemopreventive phytochemicals.
Cancer chemoprevention with dietary phytochemicalsdegarden
Young-Joon Surh
Chemoprevention refers to the use of agents to inhibit, reverse or retard tumorigenesis.
Numerous phytochemicals derived from edible plants have been reported to interfere with a
specific stage of the carcinogenic process. Many mechanisms have been shown to account
for the anticarcinogenic actions of dietary constituents, but attention has recently been
focused on intracellular-signalling cascades as common molecular targets for various
chemopreventive phytochemicals.
Anticancer Activity of Some Medicinal Plants A Reviewijtsrd
Cancer is a disease in which abnormal cells multiply in the body. It is caused by the uncontrolled multiplication and division of abnormal cells in the body. Currently medicinal plants have become the huge source of discovering drugs for treating diseases including cancer. Medicinal plants with antioxidant and anticancer properties are still in use for treating diseases. Traditional medicine have served many people globally. Chemotherapy produced a number of toxic side effects. Whereas, natural therapies such as the medicinal plants products in the treatment of cancer reduce toxic side effects. This article produce a detailed review of some medicinal plants with anticancer properties to discover a new drug. Mounica U | Pooja S | Senthil J | Janakidevi V "Anticancer Activity of Some Medicinal Plants: A Review" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-6 , October 2020, URL: https://www.ijtsrd.com/papers/ijtsrd35751.pdf Paper Url: https://www.ijtsrd.com/biological-science/biotechnology/35751/anticancer-activity-of-some-medicinal-plants-a-review/mounica-u
As the conventional therapeutic strategies fail to fulfil the major requirements for a successful cancer therapy. The use of naturally developed anticancer agents has evolved as an alternative safe, low-cost and convenient one.
chaemoprevention of cancer using dietary phytochemicals awan867
dietary phytochemicals are natural ,non-nutritive ,secondary metabolites .they are mainly for defense system in plants ,also provide colour ,aroma, flavour .they also have anti mutagenic & anti carcinogenic properties . there are 900 phytochemicals but main phytochemicals like curcumin ,gingerol,ECGC, Capsacin and many more . various cell signalling molecules can act as a target for these phytochemicals .
Dr. Patrick Hwu presents the latest information on immunotherapies for melanoma at the MRF's Patient Symposium at MD Anderson Cancer Center on January 31, 2015.
El ser humano por interacción social crea formas de obtener conocimiento sobre algo, enriqueciendose personalmente y consiguiendo habilidades que pueden sobresalir de los demás, creando así términos de superioridad o puede también que al no conseguirlo cree términos de inferioridad. Pero mayormente queriendo obtener el mando, el PODER.
Anticancer Activity of Some Medicinal Plants A Reviewijtsrd
Cancer is a disease in which abnormal cells multiply in the body. It is caused by the uncontrolled multiplication and division of abnormal cells in the body. Currently medicinal plants have become the huge source of discovering drugs for treating diseases including cancer. Medicinal plants with antioxidant and anticancer properties are still in use for treating diseases. Traditional medicine have served many people globally. Chemotherapy produced a number of toxic side effects. Whereas, natural therapies such as the medicinal plants products in the treatment of cancer reduce toxic side effects. This article produce a detailed review of some medicinal plants with anticancer properties to discover a new drug. Mounica U | Pooja S | Senthil J | Janakidevi V "Anticancer Activity of Some Medicinal Plants: A Review" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-6 , October 2020, URL: https://www.ijtsrd.com/papers/ijtsrd35751.pdf Paper Url: https://www.ijtsrd.com/biological-science/biotechnology/35751/anticancer-activity-of-some-medicinal-plants-a-review/mounica-u
As the conventional therapeutic strategies fail to fulfil the major requirements for a successful cancer therapy. The use of naturally developed anticancer agents has evolved as an alternative safe, low-cost and convenient one.
chaemoprevention of cancer using dietary phytochemicals awan867
dietary phytochemicals are natural ,non-nutritive ,secondary metabolites .they are mainly for defense system in plants ,also provide colour ,aroma, flavour .they also have anti mutagenic & anti carcinogenic properties . there are 900 phytochemicals but main phytochemicals like curcumin ,gingerol,ECGC, Capsacin and many more . various cell signalling molecules can act as a target for these phytochemicals .
Dr. Patrick Hwu presents the latest information on immunotherapies for melanoma at the MRF's Patient Symposium at MD Anderson Cancer Center on January 31, 2015.
El ser humano por interacción social crea formas de obtener conocimiento sobre algo, enriqueciendose personalmente y consiguiendo habilidades que pueden sobresalir de los demás, creando así términos de superioridad o puede también que al no conseguirlo cree términos de inferioridad. Pero mayormente queriendo obtener el mando, el PODER.
eSAC offers professional certification programs in Web Designing, Graphic Designing and Digital Marketing. The Web Designing Training Program is a 6 months comprehensive program in the fields of wireframes, web pages, responsive and HTML/CSS. The course curriculum includes advanced learning course ware prepared as per the International standards. Visit : http://www.esac.in/website-designing
Potentiale und Hindernisse für das selbstorganisierte Lernen im Web 2.0MFG Innovationsagentur
bwcon: Creative Think Net Socia "Social Media in der Weiterbildung - Lernrevolution am Arbeitsplatz?"
Vortrag: Thorsten Klein, Fraunhofer IAO
"Potentiale und Hindernisse für das selbstorganisierte Lernen im Web 2.0"
Social Media Marketing ist die Nutzung von Social Networks wie beispielsweise Facebook für Marketingzwecke. Social Networks machen Unternehmensinformationen und PR-Mitteilungen öffentlich und bringen sie schnell und direkt zu den Zielgruppen. Daher gewinnen die Netzwerke für die Unternehmenskommunikation immer mehr an Bedeutung. Social Networks ermöglichen einen Dialog zwischen Unternehmen und Kunden, weshalb Social Media Marketing mehr als klassisches Marketing im Internet ist.
Our bodies are made up of millions of cells, grouped together to form tissues and organs such as muscles and bones, the lungs and the liver. Genes inside each cell order it to grow, work, reproduce and die. Normally, our cells obey these orders and we remain healthy. But sometimes the instructions get mixed up, causing the cells to form lumps or tumors, or spread through the bloodstream and lymphatic system to other parts of the body.
Cancer is describes as the disease that results due to cellular changes and these changes cause the uncontrolled growth and division of cells. A cell receives instructions to die so that the body can replace it with a newer cell that functions better. Cancerous cells lack the components that instruct them to stop dividing and to die. Chemotherapeutic drugs CDs are the most widespread worldwide modality used in cancer treatment, and other autoimmune diseases. However, their non selective mechanism of action affects both cancerous and non cancerous cells, that resulting in well documented side effects. Nurses are at risk of suffering side effects. Little negligence or mistake may lead to adverse unpleasant effects for patients, staff and environment. Miss. Madhu Rajput | Mr. Raghavendran M "Chemotherapeutic Drugs" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd43941.pdf Paper URL: https://www.ijtsrd.com/medicine/nursing/43941/chemotherapeutic-drugs/miss-madhu-rajput
Human health has many aspects, we need to feed the diet which provides better nutrition and gives good health, an absence of disease and good behaviour. There are many health-related problems and conditions are responsible for the weak health and sometimes death causing illness, in which cancer is one most common health risk in human healthcare. Cancer is the state in which cell division is uncontrolled which damage the cells and in the last stage, if incurable caused death. There are many reasons why cell got infected due to cancerous infections, in which food habits and quality of foodstuffs are also increased the risk of cancer, like mycotoxins contamination in the food. MSG additives, taste enhancer, food colouring etc. done by the chemical processing which produces toxins inside the during the oxidation process of food. Cancer is causes of death rate higher than other health risks all over the world. The number of cancer cases is likely to rise up to 24 million by 2035. Several studies were performed in the last years in order to explore and analyze associations between diet and risk of cancer. The risk of cancer is depending on the degree of exposure to contaminated food, availability of nutrients in the body, dietary pattern and lifestyle as well as food behaviour. Research from a number of sources provides information that some vegetables, fruits, and whole grains, dietary fibre, certain micronutrients, few fatty acids and physical activity protect against cancers. Whereas poor dietary intake, imbalanced nutrition, less physical activity trances fatty acids, food processing and cooking method may increase risks.
Keywords: Cancer, death, nutrition, fruits and vegetable
Running head INEFFECTIVE CANCER TREATMENTS LEADING TO DEATHS1.docxcowinhelen
Running head: INEFFECTIVE CANCER TREATMENTS LEADING TO DEATHS 1
INEFFECTIVE CANCER TREATMENTS LEADING TO DEATHS 18
Ineffective Cancer treatments leading to deaths
Name
Course
Tutor
Date
Abstract
The main focus of the report will be to research on the various methods through which cancer can be treated with a keen eye on why some methods are ineffective and lead to death. A discussion on different cancer treatment will be done followed by the types of cancer that cause millions of death today. Presentation of answers to the research questions will be done in line with ineffective cancer treatment methods. Relevant literature review will be conducted and used to support the claims of ineffective cancer treatments. Lastly, recommendations on the best cancer treatment will be done.
Introduction
Cancer is an ailment caused by the unrestrained division of abnormal cells in the body. The cancer cells are malignant meaning they can spread from the origin to distant organs and tissues. The disease can be genetically hereditary hence can be crossed over from one generation to another. Some of the forms of cancer treatment include targeted therapy, hormone therapy, chemotherapy, precision medicine, surgery, immunotherapy, and stem cell transplant. Some of the common types of this disease include lung, liver, stomach, and bowel cancers.
Cancer is also called as malignancy which means abnormal cells growth. More than 100 types of cancer are found in this world today, including breast cancer (widely spread among women), skin cancer (found in the person of almost every age), lung cancer (common among smokers), colon cancer, lymphoma and prostate cancer. Each kind of cancer has varying symptoms. Cancer differs with respect to the cell it affects first. The uncontrollable division of cells harm the body and form lumps and the masses of tissues which are known as tumors. The tumor grows in size and sometimes even intervene the digestive system, circulatory system, excretory system and nervous system. In the case of leukemia, cancer inhibits the normal blood functioning which is caused due to the abnormal cell division into the blood stream. Cancer also causes the systems of the body to secrete hormones that alter the body functioning. Tumors that do not grow and remain limited to one spot are considered to slightly less harmful and benign. The sign of the more dangerous and malignant cells is:
1. The harmful cancerous cells move from one spot to another throughout the body using blood as a medium and invade the organs and the healthy tissues of the body.
2. These cells grow and divide rapidly, they make blood vessels of their own which are used by them in the process of feeding, called as angiogenesis.
Then comes a stage is known as metastasized in which the tumor spread successfully to the other parts of the body, penetrating into the healthy tissues of the body and damaging them badly. The process is known as metastasis. It cause ...
Studies that examined the therapeutic potential of plants leaf extracts
Plant Scientific Name Common Name Type of extraction Proposed active material
1. Solanum viarum Tropical Soda Apple Ether Solasodine glycoalkaloid
2. Acanthus illicifolious Harkucha Kanta Methanol Triterpenoids,Flavonoids,
Alkaloids
3. Annona squamosa Custard Apple Ethyl acetate Acetogenins,Alkaloids,
Dofamine
4 Alstonia scholaris. Chatium Methanol Alkaloids,Flavonoids
5. Calotropis gigantea Akanda Ethanol Triterpenoids,Flavonol
Glycosides
SOCIAL AND ECONOMIC BURDEN OF CANCER ON 2020- REVIEW Tamizhazhagan, Pugazh...Earthjournal Publisher
SOCIAL AND ECONOMIC BURDEN OF CANCER ON 2020- REVIEW
Tamizhazhagan, Pugazhendy, Sakthidasan, Jayanthi, Ki-Hyun Kim
IRO INTERNATIONAL JOURNAL OF MEDICAL AND APPLIED SCIENCES 2018, 1(1):24-30.
Gene therapy is a new tool used in combating different diseases. The majority of gene therapy clinical trials are focused on cancer and so it was no coincidence that the first commercial treatment in 2003 was for neoplasia. Currently there are a wide variety of gene therapy proposals involving a large number of anti tumour molecular mechanisms that will conceivably pave the way for highly effective a treatment options. Despite the significant advances that how been made in gene therapy in the fight against cancer, its efficacy,safety and commercial availability are still limited. Ms. Chetana D. Patil | Ms. Siddhi Chavan | Mr. Ritesh Kadam "Gene Therapy for Cancer Treatment" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26537.pdfPaper URL: https://www.ijtsrd.com/pharmacy/biotechnology-/26537/gene-therapy-for-cancer-treatment/ms-chetana-d-patil
A review on cancer therapy immunotherapy perspectivePushkar Sathe
This is a presentation on a review on present available cancer treatments. This presentation covers all the line of treatment in short. Emphasis is given on present immunotherapy and recent advances to cure cancers.
Natural Products An Emerging Therapeutic Agent For Treatment of Cancer
1. 10 | IJPRT | October – December
International Journal of Pharmacy Research and Technology
2012, Volume 2, Issue4, 10-14.
ISSN 2250 – 0944 (Online)
ISSN 2250 – 1150 (Print)
Review Article
Natural Products: An Emerging Theraputic Agents For Treatment of Cancer
Prajapati Rajendra*1
, Shah Chainesh1
, Ajudiya Keyur1
, Patel Milee1
, Patel Ayush2
1
R. H. Patel College of Pharmacy, Dahemi, Anand - 388001.
2
Sal Institute of Pharmacy, Ahmadabad.
*Corresponding Author: Email: raj199175@gmail.com
Received: 26/09/2012, Revised: 26/10/2012 Accepted: 25/012/2012
ABSTRACT
Cancer is being a life threatening disease which can prove to be fatal for an infected patient if proper care or treatment
is not taken in early stages. The cases of cancer are increasing day by day with the increase in the number of carcinogen. The
natural therapy is required to be adopted to reduce the toxicity to other cells associated with available allopathic treatment.
This review article mainly focuses on Natural products having various positive effects on the cancer cells and is very useful
in the treatment of various type of the cancer and in the death of various carcinogens. It has been proved that the natural
products show the reduction of growth of the cancer cells and other cytotoxic effect. Many of available drugs having the
capability to reduce the growth of cancer cells by 90% but having serious side effects to other cells and sometimes it may
leads to serious complications. Similarly many natural products have the ability of killing cancerous cells by 90% having
minimal or no side effects. The natural products such as beta-glucan, cat’s claw, vitamin C, mushroom extracts and various
herbs, etc are useful in the treatment of cancer and cost effective. When the proper combination of such natural products is
given it shows more potency to kill or inhibit growth of cancer cells and increase patient compliance by reducing treatment
of cost minimal side effects.
Keywords: Cancer, Carcinogen, Natural Products, Cytotoxic Effect, Cat’s Claw.
INTRODUCTION
The disease cancer is increasing at an enormous rate
and racing to the population rate. [1]
The main objective of
this review article is to understand the effect of the natural
products on the cancer cells by reduction in cell growth or
causing cell death and to acquire the knowledge if they are
more active than synthetic therapy. The Use of natural
therapy for the treatment of cancer which has very low
chances of side effects on the host cells of the human body.
Various natural products have ability to eradicate cancer
and can be used, which are easily available in a large
quantity and economical involving in unregulating cell
growth. In cancer, cells divide, rapidly and uncontrollably,
forming malignant tumors, and invade nearby parts of the
body. The cancer may also spread to more distant parts of
the body through the lymphatic system or bloodstream. Not
all tumors are cancerous, Benign tumors do not grow
uncontrollably, do not invade neighboring tissues, and do
not spread throughout the body. There are over 200
different known cancers that afflict humans. Determining
what causes cancer is complex. Many things are known to
increase the risk of cancer, including tobacco use, certain
infections, radiation, lack of physical activity, obesity, and
environmental pollutants. These can directly damage genes
or combine with existing genetic faults within cells to cause
the disease. Approximately five to ten percent of cancers
are entirely hereditary. [2,3,4]
Cancer is a disease characterized by uncontrolled
multiplication and spread of abnormal forms of the body’s
own cells. Cancer cells differs in four ways from the normal
cells. [2,4]
1. Uncontrolled proliferation
2. Dedifferentiation and loss of function
3. Invasiveness
4. Metastasis
Cancer occurs through changes in the DNA sequence
of our genome. Thus changes in the codon sequence keep in
progress as our body comes in contact with UV radiations
which can cause mutation. Cancer is mainly caused through
environmental causes like the carcinogens such as nitrogen
mustard, benzyl chloride, etc. sometimes it is caused by the
carelessness and addiction of the people like addiction of
chewing tobacco, smoking, etc and in few cases hereditary.
The environmental causes are way more in the number than
the hereditary. A mutation in signaling of the cell can send
error-causing signals to nearby cells which may cause cells
to become neoplastic, causing them to migrate and disrupt
more healthy cells. It may also cause the cell to become
immortal causing them to disrupt healthy cells forever.
Thus cancer can also be defined as continuous dividing of
the cells. Normal cells during division they pass through
various phases of cell division. During this they produce an
enzyme called telomerase which replaces the telomere. The
cells stop producing the telomerase enzyme but the
cancerous cells keep on producing this enzyme and do not
stop dividing. Normal cells do depend on their DNA
damage and cannot survive whereas cancer cells are
independent of DNA damage and thus they live further and
life span is more which creates tumors and mass.
[5,6,7,8,9,10,11]
Various class of anticancer drugs are being used
but they have severe adverse effect such as alkylating
agents such as Cisplastin is a good anticancer drug but it
has an adverse effect which leads to deafness.
Antimetabolites too have a large adverse effects such as
mucositis, thrombocytopenia, cirrhosis, etc. [2,3]
so if the
natural products are being used that would be much better
as the side effects would be less and if in any rural area is
there then it can taken as raw as the natural activity would
mostly depend on the active constituent present in that
2. Prajapati et al / International Journal of Pharmacy Research & Technology 2012 2(4) 10-14
IJPRT | October – December | 11
particular plant which we are going to use for the treatment
of cancer and some natural derivatives also helps in the
prevention of cancer as in some cases we don’t know the
etiology of the cancer because in many times the
environment causes are being seen so these chemical
constituents will help in prevention of development of any
cancer cells or preventing the normal cells from getting
infected. Almost 31 natural herbs and plants or natural
products are being used for the treatment of cancer which is
spinach, Astragalus, beta-glucan, Baikal skullcap, calcium
D-glucarate, cat’s claw, cesium, chlorophyll, Chrysin,
cordyceps, coriolus mushroom, etc[12,13,14,15,16,17]
.
Effect of Cancer on human body [2,3]
Infection due to cancer causes two genetic changes.
Out of which one is the activation of proto-oncogenes to
oncogenes due to which the control over cell division,
apoptosis and differentiation is lost. Another genetic change
which occur is inactivation of tumor suppressor genes due
to which the ability to suppress malignant change is lost.
Thus due to formation of such cells the functions of the
normal cells gets lost. The two types of tumors are being
seen- benign tumor and malignant tumor.
• Benign tumors causes destroying of hypothalamus,
atrial myxoma blocking the flow of blood through the
heart, meningiomas compressing the brain, colloid
cysts blocking the flow of cerebrospinal fluid, too much
of tumor causes a lot of pain, GI problems which causes
bleeding, potassium wasting, obstruction, hormone
production, torsion and infarction, etc
• Malignant tumors are of various types depending on
the site where they are forming and once they are
removed they are seen again but at different site. If any
of that cancer is not treated successfully it will
eventually kill the patient, causing edema, and different
type of this tumors show different effect on the body.
Natural products and their effect on the cancer cells
Generally three methods are being used for the
treatment of cancer which are surgery, radiation and
chemotherapy. In the chemotherapy generally the use of
various drugs is done such as alkylating agents, folic acid
analogs, Antimetabolites, natural derivatives, etc. now out
of all these class of anticancer drugs, the most interesting
study is done on natural derivatives. Various herbs, plants,
natural products such as spinach, Astragalus, beta-glucan,
Baikal skullcap, calcium D-glucarate, cat’s claw, cesium,
chlorophyll, Chrysin, cordyceps, coriolus mushroom, etc.
are being used in the treatment of the cancer. The
mechanism and effect of the natural products vary and so
their efficacy. Various natural products are showing the
following effect. It is estimated that there are 31 herbs and
natural products known used in treatment of cancer. [15,19]
Some of the natural products and there effects are shown in
Table: 1 as below:
Table 1: Effect of Different natural product on various types [15, 19, 20, 21]
Sr.
No.
Natural
Products
Cancer Type Effects
1.
Astragalus
saponins
Colon cancer
Inhibit cell proliferation through accumulation in S phse and G-2/M arrest,
it reduces the tumor volume as well as pro-apoptotic and anti-proliferative
effects
2. Beta-glucan Breast cancer
Obtained from mushroom and yeast and in wheat too, it helps in raising the
activity of the macrophage and NK cells in the body which helps In fighting
against the cancer cells. It also inhibits the hepatic metastases which inhibit
further growth of tumors and thus enhance long term survival of the patient.
3.
Baikal
skullcap
Leukemia, prostate,
lung cancer
Prevention of proliferation of malignant tumors, angiogenesis and causes
apoptosis.
4.
Calcium D-
glucarate
It has anticarinogenic properties in addition to glucuronidation. It generally
conjugates with the carcinogen or cancer cells and then they are excreted
through the biliary tract which is helpful during the treatment of cancer.
5. Cat’s claw Leukemia
It is helpful in treating feline leukemia virus. It is used to stimulate the
immune system. The oxindole alkaloids especially isopterodine and
pterodine stimulate phagocytosis.
6. Cesium Leukemia
Help in disappearance of pain in all patients within one to three days after
initiation of the treatment. It changes the ph from acidic to alkaline due to
which the pain sensation disappears.
7. Chrysin Breast cancer Aromatse inhibiting action which I helpful for women in breast cancer
8. Squalamine
Human ovarian
cancer, lung cancer.
Blocks blood vessels growth in tumors, it blocks the growth factor due to
which the growth of cancer cells either stops or reduces.
9. Fish oils Breast cancer
Immune system stimulation, tumor cell toxicity, alteration of cell
membrane.
10. Vitamin C
Lung cancer,
melanoma skin
cancer
It helps in killing the cancerous cells and also in inhibition of cancerous cell
growth.
Thus many such natural products such as chlorophyll,
honey, etc are useful in the treatment of cancer by cytotoxic
effect and inhibition of cell growth. The effects of the
natural products can be known and studied by performing
the following experiment where some mushroom extracts
and vitamin C is being used.
Official Bioassay of Determine the Cell Growth
Inhibition Activity of Natural Products: [22,23]
Various experiments regarding the effects of natural
products can be done in many ways but to show the effect
of natural products as they decrease the growth of the
cancerous cells. Experiment for determining the inhibition
3. Prajapati et al / International Journal of Pharmacy Research & Technology 2012 2(4) 10-14
12 | IJPRT | October – December
of cancer cell growth. The following experiment will show
the effect of natural products to inhibit of cell growth.
Certain mushroom extract act on the bladder cancer
and reduces its growth. So Bladder cancer T24 cells are
being taken from the patient infected with transitional cell
carcinoma. These cells were maintained in McCoy’s 5a
medium which consists of 10% fetal bovine serum,
penicillin (100u/ml) and streptomycin (100microgram/ml).
The culture medium is changed after every 3-4 days and the
passage of cells was performed weekly. For experiments
these cells were seeded in six-well culture plates at an
initial cell density of 1x105
cells/ml. T24 cells were cultured
for 72 hours with varying concentration of different natural
products. Cell morphology, appearance, cell number and
viability can be checked using trypan blue exclusion test
and Alamar Blue cell viability test.
Trypan Blue Exclusion Test: [22,23]
In trypan blue exclusion test, trypan blue dye is being
used in the infected cancerous cells, the viable cells uptake
the dye and which shows a clear vision of the viable cells
and thus they can be differentiated. Thus by counting the
viable cells or general movement in the vision of the cells,
reading can be recorded.
Alamar Blue cell viability test: [22,23]
In Alamar Blue cell viability test, the alamar blue dye
is used for the cell viability test. 96-well plate is being used
in this test. You can examine 50 cells per well. Serial
dilution of alamar blue is done at 1/10 volume in black,
clear bottom 96-well plates and fluorescence was measured
after 40 minutes and 18 hours. The effect of the natural
products depends on the intensity of fluorescent light
emerging from the 96-well plate. It works on the
mechanism that it contains resazurin, a non fluorescent
indicator dye, is converted into highly red-fluorescent
resorufin via redox reactions in metabolically active cells.
Thus it indicates the number of the active and living cells.
The following natural products are being tested by the
above experiment and the study of their effect on the
cancerous cells was being observed.
1) Active fraction of maitake mushroom
2) Extract of meshimakobu mushroom
3) Extract of yeast cell wall
4) Extract of Agaricus blazei Murill mushroom
5) Arabinoxylan from rice bran
6) Mixed powder of Agaricus blazei Murill mushroom and
shark cartilage
7) Mixed powder of three kinds of mushrooms and three
herbs
8) Mycelial extract of several mushrooms
9) Vitamin C
These natural products are being tested one after
another on the cancerous cells and the concentration of
them is varied too and the following effect is shown by
some of the natural products on the cancerous cells.
Sometimes to obtain better result in the effect on the cancer
cells the combination of the natural products can be given.
The results show in the following table-2.
Table 2: Results of various Natural Products with their Different Effective Concentration and Effects [24]
Sr.
No.
Natural product Effective Concentration Effect
1
Active fraction of maitake
mushroom
60 µg/ml
120 µg/ml
≥ 240 µg/ml
No effect
25% reduction in cell growth
≥ 90% reduction in cell growth
2
Extract of meshimakobu
mushroom
20 µg/ml
50 µg/ml
100 µg/ml
≥ 200 µg/ml
No effect
30% reduction in cell growth
80% reduction in cell growth
≥ 90% reduction in cell growth
3 Vitamin C
100 µM
200 µM
300 µM
500 µM
700 µM
1000 µM
No effect
No effect
25% reduction in cell growth
50% reduction in cell growth
90% reduction in cell growth
No cell growth
4 Extract of yeast cell wall 20 µg/ml ≥ 90% cell death
All the above mentioned natural products showed
reduction in the growth of cancerous cell. The other natural
products such as Extract of Agaricus blazei Murilll
mushroom, Arabinoxylan from rice bran, Mixed powder of
Agaricus blazei Murill mushroom and shark cartilage,
Mixed powder of three kinds of mushrooms and three herbs
show very little effect even when they are used in high
concentration.
Etiology [25]
Cause that is not inherited genetically, not merely
pollution.Common environmental factors that contribute to
cancer death include tobacco (25–30%), diet and obesity
(30–35%), infections (15–20%), radiation (both ionizing
and non-ionizing, up to 10%), stress, lack of physical
activity, and environmental pollutants. Substances that
cause DNA mutations are known as mutagens, and
mutagens that cause cancers are known as carcinogens.
Many mutagens are also carcinogens, but some carcinogens
are not mutagens. Alcohol is an example of a chemical
carcinogen that is not a mutagen.
Some hormones play a
role in the development of cancer by promoting cell
proliferation. Hormones are important agents in sex-related
cancers such as cancer of the breast, endometrium, prostate,
ovary, and testis, and also of thyroid cancer and bone
cancer. Excepting the rare transmissions that occur with
pregnancies and only a marginal few organ donors, cancer
is generally not a transmissible disease.
Pathogenesis [25]
Cancer occurs when a single progenitor cell accumulates
mutations and other changes in the DNA, histones, and
4. Prajapati et al / International Journal of Pharmacy Research & Technology 2012 2(4) 10-14
IJPRT | October – December | 13
other biochemical compounds that make up the cell's
genome. The cell genome controls the structure of the cell's
biochemical components, the biochemical reactions that
occur within the cell, and the biological interactions of that
cell with other cells. Certain combinations of mutations in
the given progenitor cell ultimately result in that cell (also
called a cancer stem cell) displaying a number of abnormal,
malignant cellular properties that, when taken together, are
considered characteristic of cancer, including:
• The ability to continue to divide perpetually, producing
an exponentially (or near-exponentially) increasing
number of new malignant cancerous "daughter cells"
(uncontrolled mitosis);
• The ability to penetrate normal body surfaces and
barriers, and to bore into or through nearby body
structures and tissues (local invasiveness);
• The ability to spread to other sites within the body
(metastasize) by penetrating or entering into the
lymphatic vessels (regional metastasis) and/or the blood
vessels (distant metastasis).
If this process of continuous growth, local invasion,
and regional and distant metastasis is not halted via a
combination of stimulation of immunological defenses and
medical treatment interventions, the end result is that the
host suffers a continuously increasing burden of tumor cells
throughout the body. Eventually, the tumor burden
increasingly interferes with normal biochemical functions
carried out by the host's organs, and death ultimately
ensues.
Mycelial extract of several mushrooms but when they
were used with another natural product such as Vitamin C,
it showed better effect and it helped in killing the cancerous
cells (cytotoxic effect) as shown in Table-3
Table 3: Effect Vitamin-C and Active Extracted fractions
of several Mushrooms with their effects [26]
Vitamin- C
Active
fraction of
Maitake
Mushroom
Extract of
Meshimakobu
Mushroom
Effect
200 µM 30 µg/ml -
› 90% cell
death
200 µM - 20 µg/ml
› 90% cell
death
Thus the above experiment explains the property of
natural products of inhibiting cell growth and cytotoxic
effect by causing the cell death. Some of the natural
products show the cytotoxic effect whereas some are useful
in inhibition of the growth of the cancerous cells.
Some of the above natural products such as mushroom
extract and vitamin c show effects on the cancerous cells.
Active fraction of Maitake mushroom and Extract of
Meshimakobu mushroom when inserted in the cancerous
cells shows their effect. It was observed that the intensity of
fluorescent in Alamar blue dye test was decreasing with the
increase in the concentration of the natural products which
shows that there was depletion in the growth of the cancer
cells. They mainly cause the inhibition of the growth of the
cancer cells. As the concentration of these natural products
increases, the effect increases and there in reduction in the
growth of cancerous cells to a great extent. Both of them
were required in amount of 240µg/ml and 200µg/ml
respectively for causing 90% reduction in growth of cancer
cell but when they were used in combination with vitamin
C they were used 30 µg / ml and 20 µg / ml respectively to
cause 90% cell death. Vitamin C itself doesn’t give
cytotoxic effect but in combination with active fraction of
Maitake mushroom and extract of Meshimakobu mushroom
it gives the cytotoxic effect. Therefore when used in proper
combination of natural products for the treatment of cancer,
it is proved to be more efficient and potency becomes high
whereas extract of yeast cell wall directly gives the
cytotoxic effect on the cancerous cells. Thus these natural
products can be very useful in the treatment of cancer as the
side effect is less. [26]
From the experiment which was being performed, it
was concluded that natural products do have some effect on
the cancer cells in the body of an infected person. From the
result it was shown that natural products do show two main
promising effects on the cancer cells
A. They inhibit the proliferation of the cancer cells which
results into the inhibition of the growth of the cancer
cells.
B. They show cytotoxic effect due to which the activity of
macrophage and NK cells increases which helps in
killing the cancerous cells from the body. [27,28]
CONCLUSIONS
Natural products are emerging novel approach for the
treatment of cancer and enlarge the fields of pharmaceutical
researchers. It can be predicted that the exercise of natural
product in the treatment of cancer is favorable, due to other
available treatment in the market employed with high
treatment cost and chances of severe side effects by acting
on other than cancerous cells which causes some serious
complications comparing to natural product. Evan use of
natural product in treatment of cancer is cost effective
because it is extensively available so it is affordable by
patients and hence increases patient’s compliance.
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