This slide is a brief introduction of Nano-adjuvant. This presentation includes the introduction of Nano-adjuvant, advantages of nano-adjuvant, types of Nano-adjuvant, the prospect of Nano-adjuvant, and the vaccine services of creative-biolabs.

1. Nano-Adjuvant
PRESENTED BY CREATIVE BIOLABS
www.creative-biolabs.com/vaccine

2. CONTENTS
Introduction to
Nano-adjuvant
01
Advantages of
Nano-Adjuvant
02
Types of
Nano-adjuvants
03
Prospect of
Nano-adjuvants
04
About us
05

3. Introduction to Nano-adjuvant
01

4. Nanoparticle-based adjuvants is a kind of nanoparticles with
particle size less than 1000 nm, which can enhance the immune
response of the body to antigens or change the type of immune
response.
Compared with traditional adjuvants, nano-adjuvants are favored
by vaccine researchers because of their targeting, sustained-
release, safety and high efficiency.
In particular, it shows great advantages in immune adjuvant effect
and safety, and promotes the wide application of many new
vaccines in clinical practice.
Introduction to Nano-adjuvant
www.creative-biolabs.com/vaccine

5. Advantages of Nano-Adjuvant
02

6. Advantages of Nano-Adjuvant
www.creative-biolabs.com/vaccine
2.1 Function of Adjuvant
Adjuvants have been widely used in the preparation of
vaccines. Adjuvants help the body produce a specific
immune response to antagonises.
Induce sustained immune response in children, the elderly, immune
system deficiency and other immunocompromised people.
Increase the absorption of vaccine by gastric mucosa and ingesting
antigen through mucosa.
Reduce the dosage of immune substances to the minimum
threshold of the dose required for immune protection.
Promote the infiltration of vaccine into cells and targeting antigen presentation
cell (APC), to induce cytokine release or enhance antigen presentation.
Improve the validity of antigen and enhance its immunogenicity,
immune tolerance and immune response speed.

7. Advantages of Nano-Adjuvant
www.creative-biolabs.com/vaccine
2.1 Function of Adjuvant
Adjuvants have been widely used in the preparation of
vaccines. Adjuvants help the body produce a specific
immune response to antagonises.
The abundant activation center on the surface of nano-adjuvant
can make it bind to antigen stably, and as an efficient means of
delivery, antigen can be targeted and transported.
Some nano-adjuvants also have immunostimulatory effect.
nano-adjuvants can delay the release of antigens, thus
continuously stimulating the body to produce high titers of
antibodies.
Nano-adjuvants can load antigens by encapsulation, passive
absorption, or gene fusion, thereby forming a "warehouse" for
antigens to prevent enzymatic hydrolysis or hydrolysis.
2.2 Characteristics of
Nano-Adjuvant
Nano-adjuvant has the characteristics of improving vaccine
antigen stability, immunogenicity and sustained-release
property.

8. Advantages of Nano-Adjuvant
03

9. Types of Nanoparticles
www.creative-biolabs.com/vaccine
01
Inorganic nano-
adjuvants
03
New type nano-
adjuvants
02
Biomolecular nano-
adjuvants
• Calcium phosphate adjuvant
• Nano-aluminum hydroxide
adjuvant
At present, nano-adjuvants are divided into three categories: inorganic nano-adjuvants, biomolecular nano-
adjuvants, and new type nano-adjuvants.
• Liposomes
• Virosomes
• virus-like particles
• Immunostimulating complexes
(ISCOMs)
• MF59

10. Types of Nanoparticles
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Nano-aluminum hydroxide adjuvant
Nano-aluminum hydroxide adjuvant is a kind of
particle with the size of 4.5nm × 2.2nm × 10nm, which
is formed by the aggregation of small fiber particles.
Nano-aluminum adjuvant may sometimes cause slight
inflammatory reaction at the injection site, which is
related to its immunostimulatory effect.
Calcium phosphate adjuvant
Calcium phosphate adjuvant is a kind of nanoparticles
with particle size less than 1000nm prepared by
coprecipitation and reverse microemulsion.
When calcium phosphate nanoparticles are used for
gene transfection, it may lead to the integration of
foreign genes into the host genome and reduce the
safety of the vaccine.
Inorganic Nano-Adjuvants
01 02

11. Types of Nanoparticles
www.creative-biolabs.com/vaccine
Biomolecular nano-adjuvants
Liposomes
Liposome is a hollow vesicle structure encapsulated by one or more
bilayer membranes, which has both adjuvant and carrier functions.
Advantages
I. Liposome as a carrier has the same immune effect as aluminum
adjuvant.
II. Liposome carrier is mainly composed of lipids, which is easy to be
biodegraded by the body, and can significantly reduce the
probability of inflammatory reaction at the injection site
III. Liposome as a carrier can encapsulate a large number of
antigens and has a strong repository effect.
When calcium phosphate nanoparticles are used for
gene transfection, it may lead to the integration of
foreign genes into the host genome and reduce the
safety of the vaccine.
Problems
While in the process of liposome fusion, it is easy to
cause the loss of encapsulated antigen, thus affecting
the immune effect.
Virosomes Virus-like particles

12. Types of Nanoparticles
www.creative-biolabs.com/vaccine
Biomolecular nano-adjuvants
Virosomes
Virosomes, about 150nm in diameter, is composed of circular monolayer
vesicles, which is an empty shell structure without viral nucleic acid.
Advantages
Viroids belong to microorganisms, which may replicate
themselves in sensitive hosts, causing the host to show
some symptoms of infection.
ProblemsLiposomes Virus-like particles
I. Virosomes can not only stimulate humoral immunity, but also
stimulate cellular immunity and mucosal immunity.
II. It can still induce a strong immune response in the
absence of any adjuvants.

13. Types of Nanoparticles
www.creative-biolabs.com/vaccine
Biomolecular nano-adjuvants
Virosomes
Virus-like particles are empty shell structures with diameter in 20~200nm
formed by self-assembly of one or more structural proteins.
Advantages
The limitation of virus-like particles is the loadable length
of foreign genes. When the number of amino acids in
foreign genes exceeds 300, it will affect the self-assembly
of virus-like particles into particle-like structure.
ProblemsLiposomes Virus-like particles
I. Virus-like particles are easily bound to the MHC I molecular surface
and the MHC II molecular surface, and are identified by the APC,
stimulating the body to produce a high level of humoral immunity
and cellular immune response.
II. The multivalence of virus-like particles enables B cells to
recognize specific antigen receptors that can be effectively
crosslinked and activated.

14. Types of Nanoparticles
www.creative-biolabs.com/vaccine
MF59
MF59 is a kind of oil-in-water emulsion, which is
formed by microfluidization of Sharkene (4.3%),
Tween80 (0.5%) and sorbitol trioleate Span85
(0.5%) under high pressure, and its average
particle size is less than 250nm.
Only a very small number of volunteers were
limited to the inflammatory response at the
injection site
Immunostimulating complexes
Immunostimulating complex is a compound adjuvant
which mixes antigen, cholesterol and plant saponins
QuilA at 1:1:1.
The immunogen containing more hydrophilic
groups is not suitable for the preparation of vaccine
mixed with ISCOMs.
New Type Nano-Adjuvants
01 0201 02

15. Prospect of Nano-adjuvants
04

16. Prospect of Nano-adjuvants
www.creative-biolabs.com/vaccine
Nano-
Adjuvants
It has shown many advantages, such as safety, sustained-
release, and high efficiency immune adjuvant, in a variety of
animal model experiments.
With the further development of vaccinology and the in-
depth study of the mechanism of nano-adjuvants, more
nano-adjuvants will be found and used in human and animal
vaccines.
The structure of hydrophilic nano-adjuvants can be
optimized by physical and chemical methods to
improve the antigen loading rate.
Nano-adjuvants are easy to enter the draining
lymph nodes and induce specific immune response.

17. About us
05

18. About us
www.creative-biolabs.com/vaccine
In terms of our extensive experience in delivery system development, Creative Biolabs is proud to offer our clients a series of nanoparticles
development for vaccine delivery with the best quality and most competitive price.
Vaccine Preclinical
Assessment04
Analytical Development
& Qualification02Vaccine Design01
Adjuvant
Selection03
Process Development &
GMP Manufacturing06
Vaccine Formulation
Development05

19. Nano-Adjuvant
www.creative-biolabs.com/vaccine
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-381-2994 Fax: 1-631-207-8356
Email: info@creative-biolabs.com