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Presented by
Miss. SRUTHI A B
B.Sc Agriculture
Name of the disease
Myrothecium leaf spot
©http://:cotton.ces.ncsu.edu
Occurrence:©
Brazil, Ecuador, Columbia, India, Pakistan, China.
History:
Myrothecium leaf spot was first observed on the
fields in Maranhao State, Brazil, causing yield reduction
upto 60%.
Symptoms:
o Initially, reddish spots of 0.5mm to 1cm diameter may
appear on the margin of the leaves.
o Disease symptoms are lesions with concentric
necrotic rings, with salient structures(sporodochia)
irregularly distributed.
o The affected portions fall off leaving irregular shot
holes on the leaves.
o The symptoms are observed on the petioles, bracts,
leaves and bolls.
©
©www.mississippi-crops.com
Isolation:
o The pathogen was isolated and extracted in Potato-
dextrose-agar medium.
o Thirteen isolates of M. roridum were tested, which
includes 8 from cotton and 5 from soyabean.
o Fungal structures were evaluated by using light
microscope with ocular micrometer.
o The MA-75 was the most aggressive strain.
o Spores from cotton isolates are 5,1µm × 1,5µm.
o This is the first report on Myrothecium leaf spot from
Brazilian cotton fields.
Pathogen:
o Myrothecium roridum Tode ex Fr.
o Conidia are hyalines due to dilute olivaceous, single-
celled, abundantly produced in a dark green to black
aggregate mass.
o Spores from cotton isolates are 5,1µm × 1,5µm.
Systematic position:
Kingdom: Fungi
Division: Ascomycota
Class: Sordariomycetes
Order: Hypocreales
Family: incertae sedis
Genus: Myrothecium
Species: M. roridum
Spread:
Spread by the presence of conidia present in the
infected fallen leaves.
Favourable condition:
o In vitro studies show that the maximum disease
development was reported at 25ºC±1 in both Bt cotton
and non Bt cotton crops.
o Average humidity: 84.7%.
o Rain: 11.4mm.
o pH : 6.
o Heavy during June – October.
o Dense crop canopy.
o The leaf spot incidence increased with delay in sowing
date at 15 days interval from 1-April to 1- june.
Disease management:
Resistant varieties:
No varieties released yet.
Cultural management:
Remove and destroy infected plant parts.
Biological management:
By medicinal plant extract:
Maximum inhibition ia obtained at 5% solvent
extract of Datura metal followed by Alium sativum and
Eucalyptus citriodora.
By bioagent:
Apply Trichoderma harzianum, Pseudomonas
fluorescens.
Chemical management:
o Spray Mancozeb or Copper oxychloride at 2kg/ha at
the intimation of the disease.
o Two to three sprays may be given at 15 days interval.
o In vitro studies indicated that propiconazole and cobalt
chloride at 100 and 1000 ppm conc gave 100 and
87.9% growth inhibotion of M. roridum.
o Foliar spray of propiconazole and salicylic acid at
0.1% and 0.01 % effectively controls disease.

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Myrothecium leaf spot of cotton

  • 1. Presented by Miss. SRUTHI A B B.Sc Agriculture
  • 2. Name of the disease Myrothecium leaf spot ©http://:cotton.ces.ncsu.edu
  • 3. Occurrence:© Brazil, Ecuador, Columbia, India, Pakistan, China. History: Myrothecium leaf spot was first observed on the fields in Maranhao State, Brazil, causing yield reduction upto 60%.
  • 4. Symptoms: o Initially, reddish spots of 0.5mm to 1cm diameter may appear on the margin of the leaves. o Disease symptoms are lesions with concentric necrotic rings, with salient structures(sporodochia) irregularly distributed. o The affected portions fall off leaving irregular shot holes on the leaves. o The symptoms are observed on the petioles, bracts, leaves and bolls.
  • 6. Isolation: o The pathogen was isolated and extracted in Potato- dextrose-agar medium. o Thirteen isolates of M. roridum were tested, which includes 8 from cotton and 5 from soyabean. o Fungal structures were evaluated by using light microscope with ocular micrometer. o The MA-75 was the most aggressive strain. o Spores from cotton isolates are 5,1µm × 1,5µm. o This is the first report on Myrothecium leaf spot from Brazilian cotton fields.
  • 7. Pathogen: o Myrothecium roridum Tode ex Fr. o Conidia are hyalines due to dilute olivaceous, single- celled, abundantly produced in a dark green to black aggregate mass. o Spores from cotton isolates are 5,1µm × 1,5µm.
  • 8. Systematic position: Kingdom: Fungi Division: Ascomycota Class: Sordariomycetes Order: Hypocreales Family: incertae sedis Genus: Myrothecium Species: M. roridum
  • 9. Spread: Spread by the presence of conidia present in the infected fallen leaves.
  • 10. Favourable condition: o In vitro studies show that the maximum disease development was reported at 25ºC±1 in both Bt cotton and non Bt cotton crops. o Average humidity: 84.7%. o Rain: 11.4mm. o pH : 6. o Heavy during June – October. o Dense crop canopy. o The leaf spot incidence increased with delay in sowing date at 15 days interval from 1-April to 1- june.
  • 11. Disease management: Resistant varieties: No varieties released yet. Cultural management: Remove and destroy infected plant parts.
  • 12. Biological management: By medicinal plant extract: Maximum inhibition ia obtained at 5% solvent extract of Datura metal followed by Alium sativum and Eucalyptus citriodora. By bioagent: Apply Trichoderma harzianum, Pseudomonas fluorescens.
  • 13. Chemical management: o Spray Mancozeb or Copper oxychloride at 2kg/ha at the intimation of the disease. o Two to three sprays may be given at 15 days interval. o In vitro studies indicated that propiconazole and cobalt chloride at 100 and 1000 ppm conc gave 100 and 87.9% growth inhibotion of M. roridum. o Foliar spray of propiconazole and salicylic acid at 0.1% and 0.01 % effectively controls disease.