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Muddy Microbes
Standards

                                   Process standards
 Observe and Measure; Classify; Experiment; Interpret and Communicate; Inquiry
                      Physical Science Content Standards
  Substances react chemically with other substances to form new substances with
 different characteristics (e.g., rusting, burning, reaction between baking soda and
                                        vinegar).
                     National Science Education Standards
Soil consists of weathered rocks and decomposed organic material from dead plants,
  animals and bacteria. Soils are often found in layers, with each having a different
                         chemical composition and texture.
Start with a question.
Inquiry

What processes help crude oil and natural
              gas form?
Concept

 Soil and water are full of microorganisms
   that can have a dramatic effect on the
decomposition of dead plants and animals.
This process, along with heat, pressure and
time, can create crude oil and natural gas.
Activity – Muddy Microbes

  A changing model of decomposing
 living matter reacting and providing
the element carbon which is involved
   in the formation of crude oil and
              natural gas.
Procedure

1. Remove any stones, twigs, or
     lumps from the soil.
Procedure

2. Make a compost mixture using the
   newspaper (organic), egg shells
 (carbonate), and egg yolk (sulfate)
       with a handful of soil.
Procedure

  3. Pack the compost mixture in the
 bottom of the bottle using the dowel
   rod to compact the mixture and
remove trapped air. The bottom layer
should be a uniform depth of three to
 four centimeters. (2.45cm = 1 inch)
Procedure

 4. Add soil to the bottle, using the dowel rod to
     compact the soil and remove trapped air.
Continue until the column is packed to within five
      centimeters from the top of the bottle.
Procedure

5. Cover the surface of the soil with pond water,
    filling to the lower threads of the bottle.
Procedures

6. Seal the top of the bottle tightly and place the
  bottle in a window that receives INDIRECT
SUNLIGHT. Temperature will affect the rate of
                     reaction.
Procedure

 7. Keep the bottle in the classroom and observe weekly
      for a minimum of three months. Record your
    observations. (NOTE: Watch for excessive gas
   buildup. Gas will need to be released as time
passes. When releasing gas from the bottle, do not
 remove the lid in the classroom as contents will
  produce a strong odor. Direct bottle top away
              from the body and face.)
Summary

1. What do you observe?
Summary

 The system shows decomposition, which
is one of the first steps in the formation of
                 petroleum.
Summary

How would this system relate to oil and
      natural gas formation?
Summary

Are microorganisms present?
Summary

Yes, in water from pond and soil.
Summary

How do these microorganisms
  affect the environment?
Summary

The microorganisms feed on organic
    material, decomposing it.
Summary

 5. What are the inorganic
components of this system?
Summary

Water, oxygen and rocks.
Summary

   What are the organic
components of this system?
Summary

Newspaper, eggs, microorganisms in
 pond water and soil, and bacteria.
Summary

 How do living organisms affect
the soil column? What evidence
         do you observe?
Summary

The organisms decay and form
      gas from carbon.
Summary

 This model shows that the soil and water
are not lifeless parts of ecosystems. Soil and
 water are full of microorganisms that can
have a dramatic effect on the environment.
Summary

This system of microorganisms feeds on the decomposing organic material
when oxygen is available. This process of life ensuing from life is known as
  biogenesis and is one of the first steps in oil and natural gas formation.
   Biogenesis is followed by diagenesis (non-organic reactions involving
   pressure, heat, etc.). As heat increases due to pressure created during
diagenesis, microorganism activity stops. Living organisms produce carbon
     that is vital to energy-transfer and oil and natural gas production.

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Muddy micros

  • 2. Standards Process standards Observe and Measure; Classify; Experiment; Interpret and Communicate; Inquiry Physical Science Content Standards Substances react chemically with other substances to form new substances with different characteristics (e.g., rusting, burning, reaction between baking soda and vinegar). National Science Education Standards Soil consists of weathered rocks and decomposed organic material from dead plants, animals and bacteria. Soils are often found in layers, with each having a different chemical composition and texture.
  • 3. Start with a question.
  • 4. Inquiry What processes help crude oil and natural gas form?
  • 5. Concept Soil and water are full of microorganisms that can have a dramatic effect on the decomposition of dead plants and animals. This process, along with heat, pressure and time, can create crude oil and natural gas.
  • 6.
  • 7. Activity – Muddy Microbes A changing model of decomposing living matter reacting and providing the element carbon which is involved in the formation of crude oil and natural gas.
  • 8.
  • 9. Procedure 1. Remove any stones, twigs, or lumps from the soil.
  • 10. Procedure 2. Make a compost mixture using the newspaper (organic), egg shells (carbonate), and egg yolk (sulfate) with a handful of soil.
  • 11. Procedure 3. Pack the compost mixture in the bottom of the bottle using the dowel rod to compact the mixture and remove trapped air. The bottom layer should be a uniform depth of three to four centimeters. (2.45cm = 1 inch)
  • 12. Procedure 4. Add soil to the bottle, using the dowel rod to compact the soil and remove trapped air. Continue until the column is packed to within five centimeters from the top of the bottle.
  • 13. Procedure 5. Cover the surface of the soil with pond water, filling to the lower threads of the bottle.
  • 14. Procedures 6. Seal the top of the bottle tightly and place the bottle in a window that receives INDIRECT SUNLIGHT. Temperature will affect the rate of reaction.
  • 15. Procedure 7. Keep the bottle in the classroom and observe weekly for a minimum of three months. Record your observations. (NOTE: Watch for excessive gas buildup. Gas will need to be released as time passes. When releasing gas from the bottle, do not remove the lid in the classroom as contents will produce a strong odor. Direct bottle top away from the body and face.)
  • 16. Summary 1. What do you observe?
  • 17. Summary The system shows decomposition, which is one of the first steps in the formation of petroleum.
  • 18. Summary How would this system relate to oil and natural gas formation?
  • 20. Summary Yes, in water from pond and soil.
  • 21. Summary How do these microorganisms affect the environment?
  • 22. Summary The microorganisms feed on organic material, decomposing it.
  • 23. Summary 5. What are the inorganic components of this system?
  • 25. Summary What are the organic components of this system?
  • 26. Summary Newspaper, eggs, microorganisms in pond water and soil, and bacteria.
  • 27. Summary How do living organisms affect the soil column? What evidence do you observe?
  • 28. Summary The organisms decay and form gas from carbon.
  • 29. Summary This model shows that the soil and water are not lifeless parts of ecosystems. Soil and water are full of microorganisms that can have a dramatic effect on the environment.
  • 30. Summary This system of microorganisms feeds on the decomposing organic material when oxygen is available. This process of life ensuing from life is known as biogenesis and is one of the first steps in oil and natural gas formation. Biogenesis is followed by diagenesis (non-organic reactions involving pressure, heat, etc.). As heat increases due to pressure created during diagenesis, microorganism activity stops. Living organisms produce carbon that is vital to energy-transfer and oil and natural gas production.