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Presented by-Mr . Swapnil Kale
Pharmaceutics
(M . Pharmacy sem-1st )
Department of Pharmaceutical Sciences R.T.M.N.U.,Nagpur-440033
17/4/2019
Contents
 Principle of working
 Types of mucosa
 Advantages
 Disadvantages
 Criticism
 Nono system
 Nasal/mucosal
7/4/2019 2
 A vaccine is a biological preparation that improves
immunity to a particular disease.
 A vaccine typically contains an agent that resembles a
disease-causing microorganism and is often made from
weakened or killed forms of the microbe.
 The agent stimulates the body's immune system to
recognize the agent as foreign, destroy it, and keep a
record of it.
 So that the immune system can more easily recognize
and destroy any of these microorganisms that it later
encounters.
 The terms vaccine and vaccination are derived from
Variolae vaccine (smallpox of the cow), the term
devised by Edward Jenner to denote cowpox.
7/4/2019 3
Reasons for going to mucosal delivery
for vaccines
 Mucosal surface is most common
media of infection
 Parenteral vaccines are not interact
with mucosal barrier(protective
immunity)
7/4/2019 4
 Mucosal route allows to interact antigen with
defensive mucosal layers,
 so induced mucosal immunity then kills
pathogen before reaching the systemic
circulation(next time)
7/4/2019 5
 Sublingual
 Intranasal
 Oral
 Vaginal
 Rectal
7/4/2019 6
 Induce Primary stage immunity
 Possible mass vaccination
 Systemic-mucosal response
 Needle-free
 Non-invasive
 Don't require extensive purification
 Easier production
7/4/2019 7
 Insufficient uptake(adjuvants use is the
remedy)
 Lack of human mucosal adjuvants
 Rapid clearance
7/4/2019 8
 Challenges
 Enzymes
 Barriers
 IgG release
 Stimulation
 Nanotechnology
/Remedy
 Protection
 Penetration
targeting/enhancers
 Sustained release
 Adjuvants
7/4/2019 9
Polymers
Synthetic-
Polyethylenimine , Acrylic acid derivatives.
Natural-
chitosan , hyaluronic acid , alginate, dextrin
Starch
Nano vaccines
 Needle free
 Single dose
 Thermo stable
 Adjuvant effect
7/4/2019 10
 Advantages
 Easy administration
 Highly vascularised mucosa
 Large surface area
 No enzymatic activity
 No first pass effect
 Disadvantages
 Potential irritation
 Irreversible allergy
7/4/2019 11
Contents
 Skin barriers
 Approaches
7/4/2019 12
Skin as a site for vaccine delivery
 Physical
 Enzymatic
 Immunological-epithelial defence by
keratinocytes
7/4/2019 13
Langerhans cells
(stimulate)
T cells stimulation
(produces)
Systemic IgG + IgM + IgA release
7/4/2019 14
 Liquid jet injection-(100-200 m/s speed)
eg . needle free devices
 Epidermal powder immunisation
first used in 1988
Fine power + helium gas impact
 Topical application needs adjuvant-
colloidal carrier (Nano carriers ,
liposomes)
7/4/2019 15
 Energy based approaches
electroporation ,
ultrasound and
sonophoresis
 Thermal ablation or microporation
 micro needles
7/4/2019 16
 Cordeiro, AS, Alonso, MJ (2015). Recent
advances in vaccine delivery. Pharmaceutical
Patent Analyst, in press.
 https://www.researchgate.net/publication/30
1780335_Transdermal_Delivery_of_Vaccines
 www.wiki/mucosal_delivery_of_vaccines.com
 https://www.sciencedirect.com/science/articl
e/pii/S0168365916300670?via%3Dihub
7/4/2019 17
7/4/2019 18

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Mucosal and transdermal vaccination

  • 1. Presented by-Mr . Swapnil Kale Pharmaceutics (M . Pharmacy sem-1st ) Department of Pharmaceutical Sciences R.T.M.N.U.,Nagpur-440033 17/4/2019
  • 2. Contents  Principle of working  Types of mucosa  Advantages  Disadvantages  Criticism  Nono system  Nasal/mucosal 7/4/2019 2
  • 3.  A vaccine is a biological preparation that improves immunity to a particular disease.  A vaccine typically contains an agent that resembles a disease-causing microorganism and is often made from weakened or killed forms of the microbe.  The agent stimulates the body's immune system to recognize the agent as foreign, destroy it, and keep a record of it.  So that the immune system can more easily recognize and destroy any of these microorganisms that it later encounters.  The terms vaccine and vaccination are derived from Variolae vaccine (smallpox of the cow), the term devised by Edward Jenner to denote cowpox. 7/4/2019 3
  • 4. Reasons for going to mucosal delivery for vaccines  Mucosal surface is most common media of infection  Parenteral vaccines are not interact with mucosal barrier(protective immunity) 7/4/2019 4
  • 5.  Mucosal route allows to interact antigen with defensive mucosal layers,  so induced mucosal immunity then kills pathogen before reaching the systemic circulation(next time) 7/4/2019 5
  • 6.  Sublingual  Intranasal  Oral  Vaginal  Rectal 7/4/2019 6
  • 7.  Induce Primary stage immunity  Possible mass vaccination  Systemic-mucosal response  Needle-free  Non-invasive  Don't require extensive purification  Easier production 7/4/2019 7
  • 8.  Insufficient uptake(adjuvants use is the remedy)  Lack of human mucosal adjuvants  Rapid clearance 7/4/2019 8
  • 9.  Challenges  Enzymes  Barriers  IgG release  Stimulation  Nanotechnology /Remedy  Protection  Penetration targeting/enhancers  Sustained release  Adjuvants 7/4/2019 9
  • 10. Polymers Synthetic- Polyethylenimine , Acrylic acid derivatives. Natural- chitosan , hyaluronic acid , alginate, dextrin Starch Nano vaccines  Needle free  Single dose  Thermo stable  Adjuvant effect 7/4/2019 10
  • 11.  Advantages  Easy administration  Highly vascularised mucosa  Large surface area  No enzymatic activity  No first pass effect  Disadvantages  Potential irritation  Irreversible allergy 7/4/2019 11
  • 12. Contents  Skin barriers  Approaches 7/4/2019 12
  • 13. Skin as a site for vaccine delivery  Physical  Enzymatic  Immunological-epithelial defence by keratinocytes 7/4/2019 13
  • 14. Langerhans cells (stimulate) T cells stimulation (produces) Systemic IgG + IgM + IgA release 7/4/2019 14
  • 15.  Liquid jet injection-(100-200 m/s speed) eg . needle free devices  Epidermal powder immunisation first used in 1988 Fine power + helium gas impact  Topical application needs adjuvant- colloidal carrier (Nano carriers , liposomes) 7/4/2019 15
  • 16.  Energy based approaches electroporation , ultrasound and sonophoresis  Thermal ablation or microporation  micro needles 7/4/2019 16
  • 17.  Cordeiro, AS, Alonso, MJ (2015). Recent advances in vaccine delivery. Pharmaceutical Patent Analyst, in press.  https://www.researchgate.net/publication/30 1780335_Transdermal_Delivery_of_Vaccines  www.wiki/mucosal_delivery_of_vaccines.com  https://www.sciencedirect.com/science/articl e/pii/S0168365916300670?via%3Dihub 7/4/2019 17