Venezia 31 marzo 2009 Venice Sessions 2 presso il Future Centre di Telecom Italia Maria Luisa Lavitrano presenta: Molecular medicine

1. MOLECULAR MEDICINE
…from bench to bedside
Marialuisa Lavitrano, PhD
Professor of Pathology and Immunology
Head of Molecular Medicine Lab
Department of Surgical Science
marialuisa.lavitrano@unimib.it
University of Milano-Bicocca

2. Marialuisa Lavitrano - Molecular Medicine Lab
MEDICINE - BIOTECHNOLOGY
Research & Training Projecst
Genomics
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Proteomics
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Molecular & Cell biology
cytogenetic, molecular imaging
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Pre-clinical studies
M
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Novel Therapeutic
C
Applications
S
Links with Technology platforms European
SME
EC networks Trained researchers regulations
University of Milano-Bicocca

3. Marialuisa Lavitrano - Molecular Medicine
RESEARCH ACTIVITY
Preclinical
Development Data-
Prevention/ Biological Experimental
of disease mining
Treatment validation Medicine
model
Inflammatory Diseases
Ischemia/Riperfusion Injury
Transplantation
…from bench …to bedside
Cardiovascular Diseases
Neurological Disorders
Cancer
University of Milano-Bicocca

4. Production of Transgenic animals - SMGT technology in mice
SMGT:
1989
Sperm cells as vectors for introducing foreign
DNA into eggs: genetic transformation of mice.
Lavitrano M. et al Cell; 57: 717-723.
University of Milano-Bicocca

5. Production of Transgenic animals - SMGT technology in pigs
SMGT:
2002
Efficient production by sperm-mediated gene transfer of
human decay accelerating factor (hDAF) transgenic pigs
for xenotransplantation.
Lavitrano M. et al. Proc Natl Acad Sci U S A. 2002 Oct 29;99 (22):14230-5
University of Milano-Bicocca

6. Animal models to study rejection in transplantation
2007
Reduction of hyperacute rejection and protection of
metabolism and function in hearts of human decay
accelerating factor (hDAF)-expressing pigs.
Smolenski et al Cardiovasc. Res. 2007, 73(1):143-52
University of Milano-Bicocca

7. Production of Transgenic animals - SMGT technology in pigs
SMGT: Multi-transgenic pigs
2003
Piglets add some colors to transgenic story. Nature, 424:624
2005
Multi-transgenic pigs expressing three fluorescent proteins
produced with high efficiency by sperm mediated gene transfer.
Webster et al. Mol Reprod Dev. 72: 68-76
Green filter
Blue filter
Red filter
Wide range filter
Light microscope
University of Milano-Bicocca

8. Production of Transgenic animals - SMGT technology in pigs
University of Milano-Bicocca

9. Production of Transgenic animals - SMGT technology in pigs
SMGT: Transgenic pigs
2006
non-viral
episomal
Vector
Manzini et al Proc Natl Acad Sci U S A. 2006, 21;103(47):17672-7
University of Milano-Bicocca

10. Production of Transgenic animals - Neurological disorders
SMGT:
2007
Early glial dysfunction in absence of neurodegeneration in
a Spinocerebellar Ataxia Type 1 transgenic model
Giovannoni et al Neuron Glia Biology 3, p335-351.
Conditional Transgenesis
Canzoniere et al., J Neuosci, 2004
al., Neuosci,
University of Milano-Bicocca

11. Ischemia Reperfusion Injury: protective role of CO
2004
Carbon monoxide improves cardiac energetics and
safeguards the heart during reperfusion after
cardiopulmonary bypass in pigs.
Lavitrano et al. FASEB J. 2004 Jul;18(10):1093-5.
University of Milano-Bicocca

12. Atherosclerosis: plaque prevention
2006
Indication for carotid endarterectomy versus carotid
stenting for the prevention of brain embolization
from carotid artery plaques: in search of consensus.
Biasi et al J Endovasc Ther. 2006 Oct;13(5):578-91
University of Milano-Bicocca

13. Aneurysm: gene expression profiling
2009
Gene Expression Profiling of Peripheral Blood in Patients
with Abdominal Aortic Aneurysm.
Giusti et al Eur J Vasc Endovasc Surg. 2009
University of Milano-Bicocca

14. Molecular Medicine Lab - Milano Bicocca
University of Milano-Bicocca

15. Animal Facilities & Surgical Theatre
University of Milano-Bicocca