INTENDED LEARNING OUTCOMES
At the end of this class, students should be able to:
1. Define Medical Laboratory Science and explain its relevance as a discipline.
2. Define Medical Microbiology and list its sub- specialties.
3. Define micro-organism and highlight the differences between Prokaryotes and Eukaryotes.
4. Differentiate between infection and infectious disease.
5. List and explain the modes of transmission of infectious agents.
6. List and explain the different methods of diagnosis of infectious diseases
This document provides an introduction to microbiology. It defines microbiology as the science that deals with microscopic living organisms. The study of microorganisms began after the invention of the microscope. Key figures in the development of microbiology include Antony Van Leeuwenhoek, known as the father of microbiology for his early microscopic observations, and Louis Pasteur, who demonstrated that microorganisms cause fermentation and is considered the father of modern microbiology. The document further discusses the classification, characteristics, and importance of microorganisms such as bacteria, fungi, algae, protozoa and viruses. It also outlines the scope and major divisions of microbiology.
The document discusses the history, structure, and role of medical laboratories. It begins by defining a medical laboratory and tracing the history from informal training in the 1940s-50s to modern degree programs. It describes the structure of laboratory services from community health centers up to national reference laboratories. Finally, it outlines the important role of laboratories in providing quality healthcare through accurate diagnosis, screening, and epidemiological surveillance. Medical laboratory personnel play a vital role in diagnosing disease, monitoring treatment, and ensuring optimal patient care. Laboratories also adhere to strict rules of ethics, conduct, and policies to ensure quality testing.
Analysis of used disinfectants and antiseptics correlated with the occurrence...iosrphr_editor
This document analyzes the use of disinfectants and antiseptics at a clinical hospital in Macedonia from 2007-2011 and how it correlates with nosocomial infections. It finds that the most commonly used liquid disinfectants were Betadine 10% and ethanol, while the most common solid disinfectants were formaldehyde tablets and Medicarine Nat tablets. The gynecology department predominantly used Betadine 10%. The study aims to establish a connection between disinfectant use and nosocomial infection rates to help guide prevention efforts.
Clinical bacteriology involves the diagnosis and treatment of infectious diseases through microbiological techniques. It determines the causative agents of infections, and tests the effectiveness of antibiotics against isolated bacteria. Bacteria are unicellular prokaryotes that can rapidly grow and inhabit many environments. In clinical bacteriology, physicians want to know if a specimen contains pathogens, what type, and their antibiotic susceptibility. Appropriate specimens are collected and tested through staining, culturing, and antibiotic susceptibility testing to identify pathogens and determine the most effective treatments. The final report is used by physicians to interpret and treat patients.
Biofilm is a community of microorganisms of same or different species encased in a self -produced extracellular polymeric substance on both living and non-living surfaces. Catheters are generally critical indwelling medical devices commonly used in clinical applications to enhanced flow of flu ids out of the patient’s body as well as influx of medications into human biological systems. Like many other indwelling medical devices, catheters are prone to enhanced risk of nosocomial infections which becomes a critical challenge as a result of microbial attachment to the surfaces of the catheters.
Attachment and subsequent biofilm development on catheter surfaces cause a number of persistent infections. The biofilm development increases resistance to antibiotics. However, this sometimes led to high pathogenesis, patient morbidity and mortality. This condition usually calls for a premature catheter removal which will increase the cost of treatment and improvidence of resources. This review focuses on how catheters get infected, microbial diversity among catheter biofilms, factors mediating biofilm formation on catheters and current strategies us ed in controlling biofilm formation on indwelling catheters.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
The document summarizes a study conducted over six months to identify common bacterial contaminants in a microbiology laboratory. Samples were collected from surfaces, air, hands, and clothing of laboratory personnel using culture methods and identified using biochemical and molecular tests. The most common contaminants isolated were Micrococcus (52.94% of cultures) and Bacillus subtilis (23.52% of cultures), primarily from surfaces and air, respectively. The study concludes that proper disinfection, sterilization, and personal hygiene are needed to reduce laboratory contaminants and prevent false positive culture reports and laboratory-acquired infections.
This document discusses infections associated with patients in intensive care units (ICUs). It notes that ICU patients are at high risk for infections due to their critical illness and invasive treatments like ventilators and catheters. Nosocomial infections are a major problem in ICUs, with ventilator-associated pneumonia, central line-associated bloodstream infections, and catheter-associated urinary tract infections being most common. The document provides strategies to prevent infections, including strict hand hygiene, prudent antibiotic use, aseptic technique, environmental cleaning, and education. It focuses on preventing central line-associated bloodstream infections through strategies like chlorhexidine skin antisepsis and avoiding unnecessary catheter replacements.
This document provides an introduction to microbiology. It defines microbiology as the science that deals with microscopic living organisms. The study of microorganisms began after the invention of the microscope. Key figures in the development of microbiology include Antony Van Leeuwenhoek, known as the father of microbiology for his early microscopic observations, and Louis Pasteur, who demonstrated that microorganisms cause fermentation and is considered the father of modern microbiology. The document further discusses the classification, characteristics, and importance of microorganisms such as bacteria, fungi, algae, protozoa and viruses. It also outlines the scope and major divisions of microbiology.
The document discusses the history, structure, and role of medical laboratories. It begins by defining a medical laboratory and tracing the history from informal training in the 1940s-50s to modern degree programs. It describes the structure of laboratory services from community health centers up to national reference laboratories. Finally, it outlines the important role of laboratories in providing quality healthcare through accurate diagnosis, screening, and epidemiological surveillance. Medical laboratory personnel play a vital role in diagnosing disease, monitoring treatment, and ensuring optimal patient care. Laboratories also adhere to strict rules of ethics, conduct, and policies to ensure quality testing.
Analysis of used disinfectants and antiseptics correlated with the occurrence...iosrphr_editor
This document analyzes the use of disinfectants and antiseptics at a clinical hospital in Macedonia from 2007-2011 and how it correlates with nosocomial infections. It finds that the most commonly used liquid disinfectants were Betadine 10% and ethanol, while the most common solid disinfectants were formaldehyde tablets and Medicarine Nat tablets. The gynecology department predominantly used Betadine 10%. The study aims to establish a connection between disinfectant use and nosocomial infection rates to help guide prevention efforts.
Clinical bacteriology involves the diagnosis and treatment of infectious diseases through microbiological techniques. It determines the causative agents of infections, and tests the effectiveness of antibiotics against isolated bacteria. Bacteria are unicellular prokaryotes that can rapidly grow and inhabit many environments. In clinical bacteriology, physicians want to know if a specimen contains pathogens, what type, and their antibiotic susceptibility. Appropriate specimens are collected and tested through staining, culturing, and antibiotic susceptibility testing to identify pathogens and determine the most effective treatments. The final report is used by physicians to interpret and treat patients.
Biofilm is a community of microorganisms of same or different species encased in a self -produced extracellular polymeric substance on both living and non-living surfaces. Catheters are generally critical indwelling medical devices commonly used in clinical applications to enhanced flow of flu ids out of the patient’s body as well as influx of medications into human biological systems. Like many other indwelling medical devices, catheters are prone to enhanced risk of nosocomial infections which becomes a critical challenge as a result of microbial attachment to the surfaces of the catheters.
Attachment and subsequent biofilm development on catheter surfaces cause a number of persistent infections. The biofilm development increases resistance to antibiotics. However, this sometimes led to high pathogenesis, patient morbidity and mortality. This condition usually calls for a premature catheter removal which will increase the cost of treatment and improvidence of resources. This review focuses on how catheters get infected, microbial diversity among catheter biofilms, factors mediating biofilm formation on catheters and current strategies us ed in controlling biofilm formation on indwelling catheters.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
The document summarizes a study conducted over six months to identify common bacterial contaminants in a microbiology laboratory. Samples were collected from surfaces, air, hands, and clothing of laboratory personnel using culture methods and identified using biochemical and molecular tests. The most common contaminants isolated were Micrococcus (52.94% of cultures) and Bacillus subtilis (23.52% of cultures), primarily from surfaces and air, respectively. The study concludes that proper disinfection, sterilization, and personal hygiene are needed to reduce laboratory contaminants and prevent false positive culture reports and laboratory-acquired infections.
This document discusses infections associated with patients in intensive care units (ICUs). It notes that ICU patients are at high risk for infections due to their critical illness and invasive treatments like ventilators and catheters. Nosocomial infections are a major problem in ICUs, with ventilator-associated pneumonia, central line-associated bloodstream infections, and catheter-associated urinary tract infections being most common. The document provides strategies to prevent infections, including strict hand hygiene, prudent antibiotic use, aseptic technique, environmental cleaning, and education. It focuses on preventing central line-associated bloodstream infections through strategies like chlorhexidine skin antisepsis and avoiding unnecessary catheter replacements.
Doctors Data Inc A Revolution in the Evaluation of Gastrointestinal MicrofloraBonnieReynolds4
Recent research regarding the gastrointestinal microbiome has irrefutably confirmed the fact that the
microbial inhabitants of the gastrointestinal tract, and their astonishing scope of metabolic activities,
are at the very core of health and numerous disease processes. It is also clear that clinical microbiology
testing should be optimized to address the relative abundance of all bacterial species present in a stool
specimen.
Evaluation of resistance profile of pseudomonas aeruginosa with reference to ...iosrjce
This study evaluated the resistance profile and biofilm production of 112 Pseudomonas aeruginosa isolates from clinical samples. It found:
1) Isolates exhibited high resistance to ciprofloxacin (50.89%), ceftazidime (38.39%), and ceftriaxone (34.82%), but all isolates were susceptible to imipenem.
2) 48 isolates were biofilm producers, most commonly from pus and sputum samples. Biofilm producers showed significantly higher resistance to several antibiotics compared to non-producers.
3) 17 isolates were multi-drug resistant, and most (12/17) were biofilm producers. Biofilm production was significantly associated with multi-drug resistance.
milestones of Medical microbiology-lecture notesSelvajeyanthi S
This document provides an overview of milestones in medical microbiology. It discusses important developments such as Anton van Leeuwenhoek first observing microbes in 1676, Edward Jenner developing the smallpox vaccine in 1796, Louis Pasteur designing vaccines in the 1850s, Joseph Lister introducing antiseptic surgery in 1867, and Robert Koch establishing the germ theory of disease in the 1870s-1880s. It also outlines methods used in medical microbiology like microbial culture, microscopy, biochemical tests, and serological methods. The document covers commonly treated infectious diseases, causes and transmission of infectious diseases, and diagnostic tests and treatment.
This presentation give a brief background about infection control, source of infection, what microorganisms need to grow and impact of infection on patients and healthcare system.
Nosocomial infections epidemiology and key conceptsJasmine John
Nosocomial infections, also known as hospital-acquired infections, are infections that patients acquire during the course of receiving medical treatment for other conditions within healthcare facilities. They are an important public health problem due to their frequency, associated increased morbidity and mortality, and costs. Nosocomial infections can increase patient's length of hospital stay by 5-10 days on average and double their risk of death. Strict infection control practices and standard precautions like proper hand hygiene are essential to breaking the chain of transmission between patients and healthcare workers within hospitals.
This document provides guidelines from the Infectious Diseases Society of America (IDSA) for the management of cryptococcal disease. A group of medical mycology experts reviewed and updated the previous 2000 IDSA guidelines. The guidelines discuss management of cryptococcal meningitis in three at-risk groups and make recommendations for other sites of infection. Key principles include induction therapy with fungicidal regimens followed by suppressive therapy, early recognition and treatment of increased intracranial pressure and immune reconstitution inflammatory syndrome, and use of lipid formulations of amphotericin B for patients with renal impairment. While cryptococcosis remains challenging, adherence to these guidelines can lead to successful management for most patients.
This document provides guidelines from the Infectious Diseases Society of America (IDSA) for the management of cryptococcal disease. It discusses recommendations for treating cryptococcal meningoencephalitis in 3 patient groups: HIV-infected individuals, organ transplant recipients, and non-HIV/nontransplant patients. Key recommendations include using induction therapy with a polyene and flucytosine, followed by fluconazole suppressive therapy. It emphasizes the importance of treating increased intracranial pressure and immune reconstitution inflammatory syndrome. Lipid formulations of amphotericin B are recommended for patients with renal impairment. Overall management of cryptococcosis requires a multifaceted approach considering the underlying disease, ant
Antibiotic resistance occurs when bacteria change in response to the use of these medicines. A growing number of infections – such as pneumonia, tuberculosis, gonorrhoea, and salmonellosis – are becoming harder to treat as the antibiotics used to treat them become less effective. Antibiotic resistance leads to longer hospital stays,higher medical costs and increased mortality.
This document provides an overview and introduction to a course on medical virology. It outlines the learning objectives which include gaining knowledge of biosafety guidelines, laboratory safety procedures, specimen collection and processing, cell culture techniques, and molecular detection methods for viruses. The document also acknowledges sources of images used and provides copyright information.
Basic laboratory procedures in clinical bacteriologyamin beni
book of Basic laboratory procedures in clinical bacteriology Basic laboratory procedures in clinical bacteriology Basic laboratory procedures in clinical bacteriology Basic laboratory procedures in clinical bacteriology
Infection prevention and control FULL COURSE(2).pdfmsalahabd
The document discusses infection prevention and control (IPC). It defines key IPC terms like infection, colonization, and nosocomial infections. It explains the chain of infection and how breaking the links in the chain can prevent spread. It also outlines standard infection control precautions that should be used at all times like hand hygiene and use of personal protective equipment. The document provides an overview of important IPC concepts, definitions, and best practices.
The document provides information about pathogenesis presented in a student assignment. It defines pathogenesis and lists some general terms used. It discusses virulence factors and provides examples. It describes the stages of pathogenesis as exposure, adhesion, invasion, and infection. It explains how pathogens penetrate host defenses and produce toxins. It covers transmission of disease and lists references. The skill achieved from the seminar is described as communication skills, confidence, better subject knowledge, presentation skills, and motivation.
SESSION _1_INFECTION AND PREVENTION.pptxjacob735118
This document discusses infection prevention and control (IPC) in healthcare facilities. It defines key terms like healthcare-associated infections (HAIs), standard precautions, and IPC. The objectives of IPC programs are outlined as protecting patients, healthcare workers, communities, and the environment from infection. Components of standard precautions include hand hygiene, personal protective equipment, handling sharps and waste safely. Common types of HAIs are urinary tract infections, surgical site infections, and healthcare-associated pneumonia. Risk factors for HAIs relate to the patient, healthcare environment and procedures, and patient resistance.
This document provides an overview of the laboratory diagnosis of common hospital-acquired infections (HAIs). It discusses the major causative agents and diagnostic methods for urinary tract infections (UTIs), nosocomial pneumonia, surgical site infections (SSIs), and catheter-related bloodstream infections (CRBSIs). Standard diagnostic tests include urine culture, sputum culture, wound culture, and blood culture. Microscopic examination, antigen detection assays, and molecular techniques like PCR are also used. An accurate laboratory diagnosis is important to identify the infecting organism and guide appropriate antibiotic treatment for HAIs.
Laboratory diagnosis of hospital acquired infections(nosocomial infections)Dr. Karrar Alwash
This document discusses laboratory diagnosis of common hospital-acquired infections. It begins by defining hospital-acquired infections and explaining their global impact. The most common types of infections are then outlined, including UTIs, pneumonia, surgical site infections, and catheter-related bloodstream infections. For each of these top four infections, the document discusses the main causative agents and important laboratory diagnostic methods. These include urine culture and analysis for UTIs, sputum culture and imaging for pneumonia, wound culture and blood tests for surgical site infections, and blood culture for catheter-related bloodstream infections. The document concludes by emphasizing the importance of accurate and timely diagnosis of hospital-acquired infections.
Intensive care units experience high rates of infection due to patients having more comorbidities and invasive devices, with ventilator-associated pneumonia, central line-associated bloodstream infections, and catheter-associated urinary tract infections being common; infection control strategies like hand hygiene, isolation precautions, environmental cleaning, and surveillance are effective at reducing the transmission of multidrug-resistant pathogens in ICUs and improving patient outcomes. Surveillance of device-associated infection rates and antimicrobial resistance patterns is important for guiding infection control efforts and antimicrobial stewardship in the ICU.
pathogens in periodontal microbiology. the red complex bacteria described in detail. recent updates regarding proteases and virulence factors of all pathogens.
Nosocomial infections are infections contracted by patients during a hospital stay. These secondary infections can occur as complications of the primary illness or disease being treated. Sources of nosocomial infections may be exogenous from other patients, staff, or the inanimate hospital environment, or endogenous from the patient's own normal flora. Factors that contribute to nosocomial infections include the hospital environment containing many pathogens, multi-drug resistant hospital microbial flora, instrumentation introducing infections, contaminated blood/fluids, and accidental inoculation of infectious material. Common agents causing nosocomial infections include Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa.
The document discusses key concepts of infection control including definitions of terms like nosocomial infection, disinfection, and sterilization. It also addresses factors that influence nosocomial infections like microbial agents, patient susceptibility, and environmental factors. Nosocomial infections are a major problem that increase healthcare costs and can be prevented through proper infection control programs and practices in healthcare facilities.
Test bank for karp s cell and molecular biology 9th edition by gerald karp.pdfrightmanforbloodline
Test bank for karp s cell and molecular biology 9th edition by gerald karp.pdf
Test bank for karp s cell and molecular biology 9th edition by gerald karp.pdf
Test bank for karp s cell and molecular biology 9th edition by gerald karp.pdf
Co-Chairs, Val J. Lowe, MD, and Cyrus A. Raji, MD, PhD, prepared useful Practice Aids pertaining to Alzheimer’s disease for this CME/AAPA activity titled “Alzheimer’s Disease Case Conference: Gearing Up for the Expanding Role of Neuroradiology in Diagnosis and Treatment.” For the full presentation, downloadable Practice Aids, and complete CME/AAPA information, and to apply for credit, please visit us at https://bit.ly/3PvVY25. CME/AAPA credit will be available until June 28, 2025.
Doctors Data Inc A Revolution in the Evaluation of Gastrointestinal MicrofloraBonnieReynolds4
Recent research regarding the gastrointestinal microbiome has irrefutably confirmed the fact that the
microbial inhabitants of the gastrointestinal tract, and their astonishing scope of metabolic activities,
are at the very core of health and numerous disease processes. It is also clear that clinical microbiology
testing should be optimized to address the relative abundance of all bacterial species present in a stool
specimen.
Evaluation of resistance profile of pseudomonas aeruginosa with reference to ...iosrjce
This study evaluated the resistance profile and biofilm production of 112 Pseudomonas aeruginosa isolates from clinical samples. It found:
1) Isolates exhibited high resistance to ciprofloxacin (50.89%), ceftazidime (38.39%), and ceftriaxone (34.82%), but all isolates were susceptible to imipenem.
2) 48 isolates were biofilm producers, most commonly from pus and sputum samples. Biofilm producers showed significantly higher resistance to several antibiotics compared to non-producers.
3) 17 isolates were multi-drug resistant, and most (12/17) were biofilm producers. Biofilm production was significantly associated with multi-drug resistance.
milestones of Medical microbiology-lecture notesSelvajeyanthi S
This document provides an overview of milestones in medical microbiology. It discusses important developments such as Anton van Leeuwenhoek first observing microbes in 1676, Edward Jenner developing the smallpox vaccine in 1796, Louis Pasteur designing vaccines in the 1850s, Joseph Lister introducing antiseptic surgery in 1867, and Robert Koch establishing the germ theory of disease in the 1870s-1880s. It also outlines methods used in medical microbiology like microbial culture, microscopy, biochemical tests, and serological methods. The document covers commonly treated infectious diseases, causes and transmission of infectious diseases, and diagnostic tests and treatment.
This presentation give a brief background about infection control, source of infection, what microorganisms need to grow and impact of infection on patients and healthcare system.
Nosocomial infections epidemiology and key conceptsJasmine John
Nosocomial infections, also known as hospital-acquired infections, are infections that patients acquire during the course of receiving medical treatment for other conditions within healthcare facilities. They are an important public health problem due to their frequency, associated increased morbidity and mortality, and costs. Nosocomial infections can increase patient's length of hospital stay by 5-10 days on average and double their risk of death. Strict infection control practices and standard precautions like proper hand hygiene are essential to breaking the chain of transmission between patients and healthcare workers within hospitals.
This document provides guidelines from the Infectious Diseases Society of America (IDSA) for the management of cryptococcal disease. A group of medical mycology experts reviewed and updated the previous 2000 IDSA guidelines. The guidelines discuss management of cryptococcal meningitis in three at-risk groups and make recommendations for other sites of infection. Key principles include induction therapy with fungicidal regimens followed by suppressive therapy, early recognition and treatment of increased intracranial pressure and immune reconstitution inflammatory syndrome, and use of lipid formulations of amphotericin B for patients with renal impairment. While cryptococcosis remains challenging, adherence to these guidelines can lead to successful management for most patients.
This document provides guidelines from the Infectious Diseases Society of America (IDSA) for the management of cryptococcal disease. It discusses recommendations for treating cryptococcal meningoencephalitis in 3 patient groups: HIV-infected individuals, organ transplant recipients, and non-HIV/nontransplant patients. Key recommendations include using induction therapy with a polyene and flucytosine, followed by fluconazole suppressive therapy. It emphasizes the importance of treating increased intracranial pressure and immune reconstitution inflammatory syndrome. Lipid formulations of amphotericin B are recommended for patients with renal impairment. Overall management of cryptococcosis requires a multifaceted approach considering the underlying disease, ant
Antibiotic resistance occurs when bacteria change in response to the use of these medicines. A growing number of infections – such as pneumonia, tuberculosis, gonorrhoea, and salmonellosis – are becoming harder to treat as the antibiotics used to treat them become less effective. Antibiotic resistance leads to longer hospital stays,higher medical costs and increased mortality.
This document provides an overview and introduction to a course on medical virology. It outlines the learning objectives which include gaining knowledge of biosafety guidelines, laboratory safety procedures, specimen collection and processing, cell culture techniques, and molecular detection methods for viruses. The document also acknowledges sources of images used and provides copyright information.
Basic laboratory procedures in clinical bacteriologyamin beni
book of Basic laboratory procedures in clinical bacteriology Basic laboratory procedures in clinical bacteriology Basic laboratory procedures in clinical bacteriology Basic laboratory procedures in clinical bacteriology
Infection prevention and control FULL COURSE(2).pdfmsalahabd
The document discusses infection prevention and control (IPC). It defines key IPC terms like infection, colonization, and nosocomial infections. It explains the chain of infection and how breaking the links in the chain can prevent spread. It also outlines standard infection control precautions that should be used at all times like hand hygiene and use of personal protective equipment. The document provides an overview of important IPC concepts, definitions, and best practices.
The document provides information about pathogenesis presented in a student assignment. It defines pathogenesis and lists some general terms used. It discusses virulence factors and provides examples. It describes the stages of pathogenesis as exposure, adhesion, invasion, and infection. It explains how pathogens penetrate host defenses and produce toxins. It covers transmission of disease and lists references. The skill achieved from the seminar is described as communication skills, confidence, better subject knowledge, presentation skills, and motivation.
SESSION _1_INFECTION AND PREVENTION.pptxjacob735118
This document discusses infection prevention and control (IPC) in healthcare facilities. It defines key terms like healthcare-associated infections (HAIs), standard precautions, and IPC. The objectives of IPC programs are outlined as protecting patients, healthcare workers, communities, and the environment from infection. Components of standard precautions include hand hygiene, personal protective equipment, handling sharps and waste safely. Common types of HAIs are urinary tract infections, surgical site infections, and healthcare-associated pneumonia. Risk factors for HAIs relate to the patient, healthcare environment and procedures, and patient resistance.
This document provides an overview of the laboratory diagnosis of common hospital-acquired infections (HAIs). It discusses the major causative agents and diagnostic methods for urinary tract infections (UTIs), nosocomial pneumonia, surgical site infections (SSIs), and catheter-related bloodstream infections (CRBSIs). Standard diagnostic tests include urine culture, sputum culture, wound culture, and blood culture. Microscopic examination, antigen detection assays, and molecular techniques like PCR are also used. An accurate laboratory diagnosis is important to identify the infecting organism and guide appropriate antibiotic treatment for HAIs.
Laboratory diagnosis of hospital acquired infections(nosocomial infections)Dr. Karrar Alwash
This document discusses laboratory diagnosis of common hospital-acquired infections. It begins by defining hospital-acquired infections and explaining their global impact. The most common types of infections are then outlined, including UTIs, pneumonia, surgical site infections, and catheter-related bloodstream infections. For each of these top four infections, the document discusses the main causative agents and important laboratory diagnostic methods. These include urine culture and analysis for UTIs, sputum culture and imaging for pneumonia, wound culture and blood tests for surgical site infections, and blood culture for catheter-related bloodstream infections. The document concludes by emphasizing the importance of accurate and timely diagnosis of hospital-acquired infections.
Intensive care units experience high rates of infection due to patients having more comorbidities and invasive devices, with ventilator-associated pneumonia, central line-associated bloodstream infections, and catheter-associated urinary tract infections being common; infection control strategies like hand hygiene, isolation precautions, environmental cleaning, and surveillance are effective at reducing the transmission of multidrug-resistant pathogens in ICUs and improving patient outcomes. Surveillance of device-associated infection rates and antimicrobial resistance patterns is important for guiding infection control efforts and antimicrobial stewardship in the ICU.
pathogens in periodontal microbiology. the red complex bacteria described in detail. recent updates regarding proteases and virulence factors of all pathogens.
Nosocomial infections are infections contracted by patients during a hospital stay. These secondary infections can occur as complications of the primary illness or disease being treated. Sources of nosocomial infections may be exogenous from other patients, staff, or the inanimate hospital environment, or endogenous from the patient's own normal flora. Factors that contribute to nosocomial infections include the hospital environment containing many pathogens, multi-drug resistant hospital microbial flora, instrumentation introducing infections, contaminated blood/fluids, and accidental inoculation of infectious material. Common agents causing nosocomial infections include Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa.
The document discusses key concepts of infection control including definitions of terms like nosocomial infection, disinfection, and sterilization. It also addresses factors that influence nosocomial infections like microbial agents, patient susceptibility, and environmental factors. Nosocomial infections are a major problem that increase healthcare costs and can be prevented through proper infection control programs and practices in healthcare facilities.
Test bank for karp s cell and molecular biology 9th edition by gerald karp.pdfrightmanforbloodline
Test bank for karp s cell and molecular biology 9th edition by gerald karp.pdf
Test bank for karp s cell and molecular biology 9th edition by gerald karp.pdf
Test bank for karp s cell and molecular biology 9th edition by gerald karp.pdf
Co-Chairs, Val J. Lowe, MD, and Cyrus A. Raji, MD, PhD, prepared useful Practice Aids pertaining to Alzheimer’s disease for this CME/AAPA activity titled “Alzheimer’s Disease Case Conference: Gearing Up for the Expanding Role of Neuroradiology in Diagnosis and Treatment.” For the full presentation, downloadable Practice Aids, and complete CME/AAPA information, and to apply for credit, please visit us at https://bit.ly/3PvVY25. CME/AAPA credit will be available until June 28, 2025.
10 Benefits an EPCR Software should Bring to EMS Organizations Traumasoft LLC
The benefits of an ePCR solution should extend to the whole EMS organization, not just certain groups of people or certain departments. It should provide more than just a form for entering and a database for storing information. It should also include a workflow of how information is communicated, used and stored across the entire organization.
DECLARATION OF HELSINKI - History and principlesanaghabharat01
This SlideShare presentation provides a comprehensive overview of the Declaration of Helsinki, a foundational document outlining ethical guidelines for conducting medical research involving human subjects.
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotesPsychoTech Services
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
8 Surprising Reasons To Meditate 40 Minutes A Day That Can Change Your Life.pptxHolistified Wellness
We’re talking about Vedic Meditation, a form of meditation that has been around for at least 5,000 years. Back then, the people who lived in the Indus Valley, now known as India and Pakistan, practised meditation as a fundamental part of daily life. This knowledge that has given us yoga and Ayurveda, was known as Veda, hence the name Vedic. And though there are some written records, the practice has been passed down verbally from generation to generation.
Are you looking for a long-lasting solution to your missing tooth?
Dental implants are the most common type of method for replacing the missing tooth. Unlike dentures or bridges, implants are surgically placed in the jawbone. In layman’s terms, a dental implant is similar to the natural root of the tooth. It offers a stable foundation for the artificial tooth giving it the look, feel, and function similar to the natural tooth.
Histololgy of Female Reproductive System.pptxAyeshaZaid1
Dive into an in-depth exploration of the histological structure of female reproductive system with this comprehensive lecture. Presented by Dr. Ayesha Irfan, Assistant Professor of Anatomy, this presentation covers the Gross anatomy and functional histology of the female reproductive organs. Ideal for students, educators, and anyone interested in medical science, this lecture provides clear explanations, detailed diagrams, and valuable insights into female reproductive system. Enhance your knowledge and understanding of this essential aspect of human biology.
1. MLS 211: INTRODUCTION TO MEDICAL
LABORATORY SCIENCE
BY
MLS SHUAIBU B.I
Department of Medical Laboratory Science
Faculty Of Applied Health Sciences
Edo State University, Uzairue
25/10/2022 1
3. Learning Outcomes
At the end of this class, students should be able to:
1. Define Medical Laboratory Science and explain its relevance as a discipline.
2. Define Medical Microbiology and list its sub- specialties.
3. Define micro-organism and highlight the differences between Prokaryotes
and Eukaryotes.
4. Differentiate between infection and infectious disease.
5. List and explain the modes of transmission of infectious agents.
25/10/2022 3
4. INTRODUCTION
Medical Laboratory Science is concerned with the analysis of clinical
specimen such as:
urine
blood
stool
peritoneal fluid
synovial fluid e.t.c, with the aim of identifying the cause/s of disease
conditions.
It is pivotal to the effective management of diseases.
Reports has shown that over 60 percent of decision relating to hospital
admissions, prescribed medicals and discharge of patients depends on
25/10/2022 4
5. INTROD……………
Medical laboratory science as a discipline is thus a fulcrum for practice of
modern day medicine.
One trained to perform the function above is regarded as a Medical
Laboratory Scientist or a Clinical Laboratory Scientist.
25/10/2022 5
6. INTROD……………
Medical laboratory (also called Clinical laboratory) is a facility that
provides controlled conditions in which tests are done on clinical
specimens in order to acquire information about the health of an
individual (or patient) for the purpose of:
Diagnosis
Treatment
Prevention of disease or medical research.
25/10/2022 6
7. Specialties in Medical Laboratory Science
Medical Laboratory Science has many specialties.
They include:
Medical Microbiology
Chemical Pathology
Haematology and Blood transfusion
Histopathology and
Immunology.
Different specialists work together providing valuable data for management of
25/10/2022 7
8. Medical Microbiology
Medical Microbiology is the study of micro-organisms, the diseases they cause and
host response to these diseases.
It is a branch of medical sciences that deals with the etiology, pathogenesis,
laboratory diagnosis, treatment and control of infection.
Micro-organisms are small (microscopic) organism that may exist in single or
multicellular form.
They are very small organism that cannot be seen with the naked eyes except with
25/10/2022 8
9. Medical Microbiology……………
Micro-organisms are ubiquitous and can be found everywhere and
anywhere.
They can be found in the air, soil, water, animals, and man.
Many micro-organisms are known to cause disease conditions in man.
Micro-organisms that can cause diseases are termed pathogens.
Based on cellular structure, all cells including microbes are divided into two
groups, namely:
25/10/2022 9
10. Medical Microbiology Laboratory
This laboratory deals with the study of human pathogens-Pathogens are
biological agents that cause diseases to their hosts.
They include microorganisms such as:
Bacteria
Viruses
Fungi)
Parasites (e.g. intestinal worms, lice and malaria parasites) of medical
25/10/2022 10
11. SUB- SPECIALTIES OF MEDICAL MICROBIOLOGY
These sub-specialties are:
1. MedicalVirology: Focuses on the study of viruses and the diseases they
cause in man.
2. Medical Bacteriology: Focuses on the study of bacteria and the diseases
they cause in man
3. Medical Mycology: This focuses on the study of fungi that causes disease
in man.
4. Medical Parasitology: Focuses on the study of parasitic diseases in man
25/10/2022 11
12. INFECTION AND INFECTIOUS DISEASE
Infection is the invasion of a tissue by micro-organisms (bacteria, viruses,
fungi etc).
An infectious disease is a disorder resulting from the invasion and
colonization of tissue by micro-organism (bacteria, fungi, viruses etc).
One with an infectious disease typically presents with signs and symptoms
of illness.
25/10/2022 12
13. Cont.……
Based on place of acquisition, infection can be classified as
1. Nosocomial infection. This is sometimes called Health-care associated
infection.
This is an infection that is acquired in a hospital or any other health care
facility such as clinics, maternity homes, rehabilitation homes etc.
Infection can be spread to a susceptible host in a clinical setting through
contaminated equipment, beddings, health care staff, another patient, or
even in some cases from the patient’s own microbiota, particularly after
surgery.
2. Community acquired infection: This is any infection acquired outside a
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14. MODE OF TRANSMISION OF INFECTIOUS AGENTS
There are a number of ways through which infectious agents can be
transmitted to cause disease. These are;
1. Human to human contact: This involves direct body contact with that of
an infected person leading to transfer of infective agent.
2. Feacal oral route: This involves the ingestion of materials (food, water)
that have been contaminated with infective micro-organisms.
3. Vertical transmission: This refers to placenta transfer of infectious agents
from mother to child.
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15. Cont.…………
4. Aerosol: Transmission can be through infective airborne droplets.
Droplets are formed from infected persons during actions like coughing ,
sneezing and talking.
Inhalation of these droplets that contain infective micro-organism by
susceptible host can cause infection.
5. Vector borne transmission: This occurs when certain vectors such as
mosquitoes, rats, fly etc, transmit infective micro-organism to susceptible
host.
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16. DIAGNOSIS OF INFECTIOUS DISEASES
Effective management of infectious diseases depends largely on the timely and
accurate diagnosis of its etiologic agents. This can be achieved by the use of
one or a combination of the following laboratory methods
Microscopy: This involves the use of microscope in viewing clinical specimen
in other to reveal the presence of inherent micro-organism that may be the
cause of disease.
Serological Technique: This relates to procedures that detects specific
antigens or antibodies of pathogens in clinical specimens of patients.
Cultural method: This employs the use of appropriate culture media in
growing and identifying the pathogen in the laboratory.
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17. Cont.…………..
Biochemical tests:
These are tests used to confirm the identity of pathogens in the Laboratory.
Laboratory.
Common biochemical test used in the laboratory include citrate utilization
test, coagulase, catalase, indole tests, urease test, oxidase tests and vogue-
proskauer tests.
Nucleic acid detection (Polymerase Chain Reaction):
This is an amplification technique that allows the detection and selective
replication of a targeted portion of a genome.
Basically, the procedure sets out to amplify small quantity of microbes into
several million copies, followed by the detection of nucleic acid on a
specialized medium.
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18. • Haematology: This laboratory is involved in the performance of
relevant tests (on blood) in the diagnosis of blood diseases, (e.g.
Anaemia, Haemoglobinopathies, Leukaemia etc) and blood
transfusion services e.g. blood group, blood cross matching.
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19. • Chemical Pathology Laboratory (Clinical Chemistry or Clinical
Biochemistry Laboratory): This division of laboratory is concerned
with the performance of quantitative and qualitative tests on clinical
specimens to investigate the state of various body chemistries.
• Such clinical specimens include body fluids (e.g. whole blood, plasma,
serum, urine, sweat, cerebrospinal fluid) and occasionally faeces,
tissue, hair e.t.c.
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20. • Histopathology Laboratory: This is the laboratory where tissues (or
cells) are processed for microscopic examination in order to
investigate or study disease manifestations on the tissue (or cells),
structure, for diagnostic purposes e.g. Cancer diagnosis.
• In the laboratory, tissue samples are processed onto glass slides from
which effects of diseases on the histological architecture of tissues
can be microscopically examined and hence diagnostic inferences are
made e.g. Cancer diagnosis.
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