The use of probiotics in supplements and foods is increasing. Probiotics are live microorganisms that provide health benefits when consumed in adequate amounts. They help maintain a balance of beneficial bacteria in the gut. However, manufacturers must address challenges to ensure probiotics survive processing and storage. Probiotics are sensitive to moisture, heat, air and light. Strict controls and short transit times are needed. Products should be labeled with colony-forming units and stored and shipped under refrigeration to maintain viability until consumed.