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Methods of detection of
Helicobacter pylori
Naimenur KORKMAZ 4th course 2nd group
What is helicobacter pylori?
• Helicobacter pylori is a type of bacteria that infects the
lining of the stomach and upper intestine.
• It's spiral-shaped and is known to be a major cause of
various gastrointestinal disorders, including gastritis
(inflammation of the stomach lining) and peptic ulcers
(sores in the lining of the stomach or duodenum).
• H. pylori is primarily transmitted through oral-oral or
fecal-oral routes.
• It's estimated that over half of the world's population is
infected with this bacterium, though not everyone who
is infected will develop symptoms or complications.
• There are several methods to detect the presence
of H. pylori in a person. These methods include:
BLOOD TESTS
• Serology: This test looks for
antibodies in the blood that the
body produces in response to an
H. pylori infection. However, it's
worth noting that serology tests
can remain positive even after the
bacteria have been eradicated.
STOOL TESTS
• Antigen Tests: These tests detect H. pylori proteins in a stool sample. They are a reliable way to
confirm the presence of the bacteria and monitor the effectiveness of treatment.
UREA BREATH TEST
• In this test, the patient swallows a capsule or liquid that contains a special type of urea labeled
with a radioactive or non-radioactive marker. If H. pylori is present, it will break down the urea
and release the marker, which can be detected in the breath.
ENDOSCOPY
• Biopsy: During an endoscopy, a thin, flexible tube with a
camera at the end is passed through the mouth and into the
stomach. If H. pylori is suspected, the doctor will take small
tissue samples (biopsies) from the lining of the stomach for
analysis. These biopsies are then examined under a
microscope to check for the presence of H. pylori.
RAPID UREASE TEST
• A small sample of tissue from the stomach lining is taken during an endoscopy and placed in a
special solution that contains urea. If H. pylori is present, it will produce an enzyme (urease) that
breaks down the urea, leading to a change in the color of the solution.
PCR (POLYMERASE CHAIN REACTION)
• PCR is a molecular biology technique that amplifies specific DNA sequences. It can be used to
detect the DNA of H. pylori in samples taken from the stomach lining.
CULTURES
• Culturing involves taking a sample from the
stomach lining and attempting to grow H.
pylori in a laboratory. This method is less
commonly used due to its complexity and
the time it takes to obtain results.
• Histology:
• This involves examining tissue samples
under a microscope to look for signs of H.
pylori infection, such as inflammation and
bacterial presence.
Sources
• https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4616200/
• https://link.springer.com/article/10.1007/s10096-018-3414-4
Thank you

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Methods of detection of Helycobacter.pptx

  • 1. Methods of detection of Helicobacter pylori Naimenur KORKMAZ 4th course 2nd group
  • 2. What is helicobacter pylori? • Helicobacter pylori is a type of bacteria that infects the lining of the stomach and upper intestine. • It's spiral-shaped and is known to be a major cause of various gastrointestinal disorders, including gastritis (inflammation of the stomach lining) and peptic ulcers (sores in the lining of the stomach or duodenum). • H. pylori is primarily transmitted through oral-oral or fecal-oral routes. • It's estimated that over half of the world's population is infected with this bacterium, though not everyone who is infected will develop symptoms or complications. • There are several methods to detect the presence of H. pylori in a person. These methods include:
  • 3. BLOOD TESTS • Serology: This test looks for antibodies in the blood that the body produces in response to an H. pylori infection. However, it's worth noting that serology tests can remain positive even after the bacteria have been eradicated.
  • 4. STOOL TESTS • Antigen Tests: These tests detect H. pylori proteins in a stool sample. They are a reliable way to confirm the presence of the bacteria and monitor the effectiveness of treatment.
  • 5. UREA BREATH TEST • In this test, the patient swallows a capsule or liquid that contains a special type of urea labeled with a radioactive or non-radioactive marker. If H. pylori is present, it will break down the urea and release the marker, which can be detected in the breath.
  • 6. ENDOSCOPY • Biopsy: During an endoscopy, a thin, flexible tube with a camera at the end is passed through the mouth and into the stomach. If H. pylori is suspected, the doctor will take small tissue samples (biopsies) from the lining of the stomach for analysis. These biopsies are then examined under a microscope to check for the presence of H. pylori.
  • 7. RAPID UREASE TEST • A small sample of tissue from the stomach lining is taken during an endoscopy and placed in a special solution that contains urea. If H. pylori is present, it will produce an enzyme (urease) that breaks down the urea, leading to a change in the color of the solution.
  • 8. PCR (POLYMERASE CHAIN REACTION) • PCR is a molecular biology technique that amplifies specific DNA sequences. It can be used to detect the DNA of H. pylori in samples taken from the stomach lining.
  • 9. CULTURES • Culturing involves taking a sample from the stomach lining and attempting to grow H. pylori in a laboratory. This method is less commonly used due to its complexity and the time it takes to obtain results. • Histology: • This involves examining tissue samples under a microscope to look for signs of H. pylori infection, such as inflammation and bacterial presence.