Nilesh Kamble presented a seminar on the development and validation of a method for the enantiomeric estimation of omeprazole enantiomers in enteric-coated formulations using high-performance liquid chromatography. Omeprazole exists as two enantiomers but the S-enantiomer is metabolized more slowly and at lower doses. The method was developed using a chiral column with normal phase chromatography. Validation showed the method was specific, stability-indicating, linear, accurate and suitable for quantifying the undesired enantiomer in formulations. The developed method can help ensure only the desired enantiomer is present in marketed formulations.