Preservation of biological samples at cryogenic temperatures - Conservazione di campioni biologici a temperature criogeniche

1. Robotic system to store biological samples
at cryogenic temperatures
SMARTFREEZER®
Angelantoni Life Science S.r.l. – All Rights Reserved - © 2013

2. Biological sample storage

3. Samples are useful as long as they are stored
reliably, without deterioration, protected from
accidental and intentional physical damage.
Biological sample storage

4. The samples are precious
What researchers think about their vial content:
• Months of hard work
• Unique patient sample
• A lot of money spent
• Many people involved
Current limits of traditional systems

5. Traditional storage systems
Liquid Nitrogen Tank
(-196°C liquid phase,
-150 °C vapor phase)
Ultra low temperature
freezers, -80°C

6. Recent Survey to Sample Quality (M. Baker, Nature: 486:141–146)
Sample quality

7. The most Biobanks in institutes, clinics and
universities are full of useless specimens. Several
billon Dollars are waste in the last years
(Carolyn Compton, Biobank National Cancer Institute)
High deficits at existing biobanks

8. Decrease of sample quality! A sample without data link is lost!
Literally looking for the
needle in the hay.
Temperature change
at worst thawing
Loss of sample
Data and / or location
Key issues

9. Even a precise workflow of
99,8%
Problems in Biobanking Processes
10 / 50,000

11. The only completely automated device for the
storage of biological samples at cryogenic
temperature (-180 °C, LN vapor phase).
Smartfreezer ®®
 Error free
 Processing time
 Staff costs
 Sample tracking
Precision of 100%

12. Automation Room
Humidity free area,
ambient temperature
Detail of the gripper to
handle vials
Storage Room
Dry LN2 vapour phase,
cryogenic temperature
Detail of a disc to store vials
inside LN2 tank
Electronic Room
Detail of the touch screen user
interface
Liquid Nitrogen and Air
Connections Room
Detail of the LN2 line connector
Loading/unloading
for cryotubes
Smartfreezer ®®

13. Software
 User friendly ouch Screen Interface
 Easy searches through queries
 Full traceability of loading/unloading (complete
audit trail)
 Automatic tracking of the sample, historical data
and inventory
 Seamless integration with LIMS (Laboratory
Information Management System)
 FreezerPro is certified according to FDA 21 CFR part
11

14.  Individual identification of the sample by dot code or
barcode
 Pneumatic gripper
 Withdrawal of a single sample (single picking/cherry
picking)
 No extra storage required
Single picking of vials

15.  Fully automated system
 Controlled humidity
 Separation between storage volume and automation
 Pressurized automation compartment
 Up to 14.470 tubes of 1.0 ml in LN2
 Up to 8.410 by 2.0 ml vials in LN2
Automation

16.  Computer Vision aided setup and
maintenance activities
 In case of unexpected events, the
function "Vision" allows to perform
alignment between the storage
positions and the gripper.
SW interface and on board PC

17. Thank you for your
attention!
www.angelantonilifescience.it
Angelantoni Life Science S.r.l. – All Rights Reserved - © 2013