Match each term with its description or main characteristic(s). A. Seguencing by repication of ONA by DNLA polymerase and delection of the incorporation of Sanger dNTPe inte the groweg DNA chain B. Identilying the genome of a novel (ie for the frst time) beganism nbere no raferense gensme. is avaluble far mapping C. Herkable changes in gene acsivy caused by mechanisms other than DNA sequnca changes: D. A peivate inscoustoc that pioneered (and competed with Nit) the sequencing af Human Genome Project which was "completed" in 2001 C. A subset of repions of inerest are sequenced ts acheve much higher ceverage than achiered in WGS 6. Uied in lilumaina SBS technology to detect wach base as at is incorporatid into ONA. Their use mininizes incerperation tas and greatly reduces raw anger rates compared to ether technoldgies v Multipiexing C. In mutplexed Berades, missssignment of reado to the leraries. V Index hopping 14. Unicue barcoce sequences can be added to each Deci fragment se that each read eas then be liset esed and sorted by these unique sequences. This olloss a large number of samples io WaS be pealed and sequeeced simulaneously in a single sequanchg nin. Exome sequencing sesuencing of the entien gthame of as organism. - De novo sequencing 5. In ordec to idecsty the regions of ONA that a transcripton factor tinds to. a chronath Byesenciecpilasea is done io pull down the proteh of intarest and any bound bNA regiont. - v Contig folened by kos of these ONe tragments. K Segjesdiag depth can be selected to detect loe frequency mutafons mithin a mixed cel * Targeted sequencing pobdiain er to detect high frequency geneme mide variacts - Eplgenetics C. Chan aermination method of ONA sequences kented h 197 . W ieles an 5 . deacy reciesedeb bhesphates (deNTPa) to lerrinale chain elengation and the readout of the * v Methylation sequencing ienshe of these terminated hagments on a get * ChP sequencing edepter foetos. Taese adapter legted tregments are PCR ampetied and gel purifed 1. Cnebles mece accurata read algament and the ablity to deted indels Allos the allynment plocritere to ule the informaton about diatance betiretn these shert sequences P. A centiguous segrent of ONA cosstructed tom overlapeing reads a Irvestigatso of Cpiosine methylation states. Cytotin methylafion in promster and heteroctiomatin regese can zigrificanty athe gene regulation Match each term with its description or main char Sanger Celera Library prepration SBS Reversible terminators Paired-end sequencing Tunable coverage Multiplexing Index hopping WGS Exome sequencing De novo sequencing Contig Targeted sequencing Epigenetics Methylation sequencing ChIP sequencing A. Sequencing by replication of DNA by DNA polymerase and detection of the incorporation of dNTPs into the growing DNA chain. B. Identifiying the genome of a novel (ie. for the first time) organism where no reference genome is available for mapping. C. Heritable changes in gene activity caused by mechanisms other tha.