The document discusses the development and production of cultured meat, or meat grown from animal cells in vitro, as a more sustainable alternative to traditional livestock farming. It explores the process by which muscle cells are cultured and stimulated to grow into tissues and compares the resource usage and costs of cultured meat production to conventional meat production. The document also examines public perceptions of and willingness to purchase cultured meat products.

1. Redesigning meat
Mark Post, Physiology, Maastricht
@MarkPost6
A Great Day for Food Innovation, Danish Food Cluster, Aarhus 2016

2. land
water
Fossil fuel
energy

3. Human trophic level
Bonhommeau et al, PNAS, Dec 2013
Medianhumantrophiclevel
GDPpercapita(USD)
1960 1970 1980 1990 2000 2010
2.052.152.252.35
2.0 2.2 2.4 2.6
World
World without China and India
China and India

8. 3 wk

11. efficiency mimicrysustainability
Van Gogh Norris

12. =30 x 109 satellite cells
=100
=25,000 ltr
=40,000

13. No
coating
Low
conc
High
conc
Day 7

14. Life cycle analysis
% land % water % energy
meat
total
meat
total
cultured
livestock

15. sustainability
serum
other animal derived products
recycling

16. Serum free media
0,246 0,015
0,056 0,016
0,170 0,010
0,189 0,012
0,272 0,013
0,156 0,006
0,177 0,011
0,207 0,004
0,149 0,009
0,155 0,012
0,186 0,010
0,166 0,006
0,196 0,002
0,245 0,011
0,175 0,007
0,200 0,005
0,177 0,005
0,187 0,008
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
GM
6%Xerumfree+1%P/S/A
6%Xerumfree+Mix+1%P/S/A
GM
GM+mix
6%Xerumfree+1%P/S/A
6%Xerumfree+Mix+1%P/S/A
GM25%mediumeringelaten
6%Xerumfree+1%P/S/A25%mediumer
ingelaten
6%Xerumfree+Mix+1%P/S/A25%
mediumeringelaten
6%Xerumfree+Mix+1%P/S/A
ingevrorenaliquots
1%lipogro+1%P/S/A
1%lipogro+Mix+1%P/S/A
2%lipogro+1%P/S/A
2%lipogro+Mix+1%P/S/A
5%lipogro+1%P/S/A
5%lipogro+Mix+1%P/S/A
GM
6%Xerumfree+1%P/S/A
6%Xerumfree+Mix+1%P/S/A
GM25%mediumeringelaten
6%Xerumfree+1%P/S/A25%mediumer
ingelaten
6%Xerumfree+Mix+1%P/S/A25%
mediumeringelaten
6%Xerumfree+Mix+1%P/S/A
ingevrorenaliquots
no medium change day 2 medium change serum change every day
Absorption(AU)
Day 1
Day 4

17. mimicry
taste
texture
color

18. MPC differentiate better than C2C12
M.Langelaan, J Tissue Eng Regen Med, 2011

19. Color, Fe2+?
0
1
2
3
4
5
6
7
0 10 20 30
Relativemyoglobin
expression
Caffeine concentration (mM)
Myoglobin expression in bovine satellite cells
Normoxic
Hypoxic

20. Other than skeletal muscle cells

21. creating fat tissue (preferably containing
ω-3, ω-6 fatty acids)
0
0,05
0,1
0,15
0,2
0,25
0,3
0,35
0,4
0,45
Fatcontent(AU)
no insulin

24. © Next Nature Network, Bis publishers
DIY !

25. ?

26. Y
Y
Y
Y
Y
YY
Y
Y Y
Y
Y
Y
63%

27. 63%
37%
Are you in favor or
against in vitro
meat (independent
of whether you’d
buy it)?
in favoragainst
23%
29%23%
13%
12%
Would you buy in
vitro meat if it was
for sale in the local
supermarket?
yes,
sure
yes,
probably
perhaps
prob.
not
no

30. $

31. TEB Master Cell Bank Production

32. Outcomes of the modeling
Cells produced per single batch 1.75E+14
Cells per gram of muscle tissue 5.00E+06
Mass of beef produced in kgs 3.50E+04
Cost per kg of beef production $ 65.57
Population fed by single batch 815
Cost per single human being fed $ 2,815.87
Population fed by single bioreactor (13 cycles per yr) 10595
data: J. Rowley