DNA can form loops and coils called supercoils. There are two types of supercoiling - positive and negative. Positive supercoiling occurs when DNA is coiled tightly in a right-handed direction, while negative supercoiling occurs when DNA is coiled in a left-handed direction. The degree of supercoiling is represented by linking number, twist, and writhe. Enzymes called DNA topoisomerases regulate supercoiling by cutting and rejoining DNA strands to reduce tension. There are two classes of topoisomerases - Class I cuts one strand while Class II cuts both strands.