Superlooping in DNA relieves torsional strain energy and affects gene expression by compacting DNA. Several mathematical models describe superlooping mechanics but lack experimental validation. This study aims to verify T. Strick et al.'s model relating applied force, polymer properties, and first superloop radius using a fishing line to simulate DNA. Twisting tests used varying masses to induce superloops. Results show the twist at first superloop correlates to initial length and the radius is independent of length. The final length after unwinding correlates linearly with initial length. Experimental radii differ from theoretical values by a factor of 1.304, partially validating the model.