The cell cycle is regulated through various signaling pathways that use different mechanisms for signal transduction, including competitive inhibitors and dimerization. The EGF pathway regulates cell division and is often mutated in cancers, utilizing EGF to dimerize receptors and transduce signals. Common techniques to analyze cell size and molecular weight include separating DNA by charge and size-based separation that can distinguish wild type and mutant samples.

1. Lecture #4

2. How cell cycle is regulated?

5. Signaling pathway
Different signaling pathways use different mechanisms for signal transduction.
Those mechanisms have different kinds of on/off switches

6. Competitive inhibitor

11. EGF pathway regulates cell division, and is mutant in many cancers.
Epidermal Growth Factor (EGF) pathway

13. Dimerize—dimer

31. 2 common techniques

46. DNA is negatively charged

47. WT: 1000kDa, larger
Mutant: <1000kDa, smaller