The document discusses three layers of communication on Twitter: the macro level of hashtags (#hashtags), the meso level of follower networks, and the micro level of direct messages (@mentions). These layers interact and information can transition between them through retweets. Studying them individually provides only a limited understanding, as the layers intersect and public discussions on Twitter are interwoven with the wider media environment. More research is needed on how publics form and interact on Twitter, how communication patterns on Twitter relate to the broader society, and how to apply social media research methods beyond any single platform.