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LACRIMAL
FUNCTION TESTS
Dr. Anshumalee Patel
TEAR FILM ADEQUACY
• Schirmer tests
• Tear film Break Up Time
• Fluorescein stain
• Rose Bengal stain
• Lissamine green
TEAR FILM ADEQUACY
• Marginal tear strip
• pH
• Tear lyzozyme level
• Tear lactoferrin level
• Tear evaporation
TEAR FILM ADEQUACY
• Tear film osmolarity
• Conjunctival biopsy and scrapings
• Conjunctival impression cytology
• Fluoophotometry
Schirmer tests
• Whatman filter paper 41
• 5*35 mm
Schirmer I
• Tests both basal and reflex secretion
Reading at 5 mins Inference
10 -15 mm Normal
5 - 10 mm Diminished
<5 mm Severe dry eye
Basal secretion test
• Conjuctiva is anesthetised
• Does not test reflex secretion
Schirmer II test
• Schirmer test performed after rubbing
nasal mucosa with dry cotton swab
• Wetting noted after 2 mins
Schirmer III test
• Pt looks directly in the sun
• Tests reflex secretion
TEAR FILM BREAK UP TIME
• Interval b/w complete blink and
appearance of dry spot on cornea
• F-stain > observed in slit lamp
• Normal: >/= 10 seconds
Rose Bengal stain
Stains red
• Mucus
• Debris
• Devitalized cells
Very irritating
Rose Bengal stain
• Pattern A
Confluent stain on
exposed bulbar
conjunctiva + blotchy
and confluent on
cornea
Rose Bengal stain
• Pattern B
Excessive staining of
exposed area
Rose Bengal stain
• Pattern C
Fine punctate stains
in the interpalpebral
area
Lissamine green stain
Stains green
• Mucus
• Debris
• Devitalized cells
Less irritating than rose bengal
Marginal tear strip
• Tear meniscus - continuous, full and
concave meniscus, b/w eyelid margin
and inferior bulbar conjunctiva
Height > 0.5 mm = NORMAL
pH
• Slight alkaline shift in dry eye
Tear lysozyme levels
• Bacteriolytic enzyme
• Tubuloacinar cells of lacrimal glands
Decreased in dry eye
Tear film osmolarity
3.02 + 6.3 mOsm/l
• Becomes hypertonic in KCS
• Specific diagnostic test for KCS
Conjunctival biopsy
• Goblet cell
density
• Keratinisation of
epithelial cells
Grading
Stage 0: Normal cellular structure
Stage 1: Early loss of goblet cells
Stage 2: Total loss of goblet cells
Stage 3: Early and mild keratinisation
Stage 4: Moderate keratinisation
Stage 5: Severe keratinisation
WATERING EYE
• ROPLAS test
• FDDT
• Lacrimal syringing test
• Jones tests
• DCG
• Lacrimal scintillography
ROPLAS
Regurgitation on
pressure of Lacrimal
sac
Mucopurulent
discharge - Chronic
dacryoscystitis
Fluorescein Dye Disappearance test
• Drop instilled in conjunctival sac
• Observed after 5 mins
• Color intensity after 5 mins: 0 to +4
• +2 to +4 > Inadequate drainage
Lacrimal syringing test
• TA - 2 to 4% xylocaine
Result Inference
Free passage No obstruction
Passes with considerable
pressure on syringe
Partial obstruction
Reflux through same punctum Obstruction in same canaliculus
Reflux through other punctum
Obstruction in common
canaliculus, lacrimal sac, NLD
Primary Jone’s test
• 1% fluorescein dye placed in
conjunctival sac
• Cotton bud dipped in xylocaine +
epinephrine is placed in inferior
meatus
Inference
• Stained bud - Adequate drainage:
Watering is due to hypersecretion
• Unstained bud - Inadequate drainage:
Obstruction
Failure of lacrimal pump
Secondary Jone’s test
• Residual fluorescein is flushed from
conjunctival sacs
• Lacrimal syringing done with clear
saline
Inference
• Stained bud - normal lacrimal pump,
partial obstruction
• Unstained bud - lacrimal pump failure
Dacryocystography
• Radio-opaque dye:
Lipiodol
Pentopaque
Dianosil
Conray-280
• Xray taken at 5 & 30 mins
Dacryocystography
• Exact site,extent and nature of block
• Information about mucosa about sac,
fistula, diverticula, stone or tumor of
lacrimal passage

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Lacrimal function tests.pptx