Laboratory safety measures include: 1. Always wear protective clothing and tie back long hair. 2. Clean and disinfect all surfaces before and after use. 3. Never eat, drink, smoke or place objects in your mouth in the laboratory. 4. Properly label and handle all cultures, samples and reagents. Dispose of materials safely and do not allow organisms to leave the laboratory.

1. LABORATORY SAFETY
MEASURE

2. 1. Always wear an apron before entering the laboratory
2. Clean your workbench before and after laboratory work with disinfecting
solution. (with phenol 1:100 or 90% alcohol)
3. Never smoke, eat, or drink in the laboratory.
4. Never place pencil labels or any other materials in your mouth.
5. If live culture is spilled cover the area with disinfectant for 15 mins and then
clean.
6. In the event of personal injury such as a cut or burn inform your instructor
immediately as bacteria enter into open wounds.

3. 7. Long hair should be tied back to minimize contamination of culture and fire hazards.
8. All cultures and prepared solutions should be properly labeled or marked.
9. Before using any culture ensure that you are using the right culture.
10. If any culture has to be used see that further stock of culture is available for future work
before giving culture please check for purity.
11. Before discarding any culture, the organism must be killed. Never allow the
organisms to go out of the laboratory.
12. Do not allow the culture whether it Petri dish or in tubes or flasks left open. It
remains open, mites may pick up the colonies and spread the whole laboratory.
13. Put off the burners when they are not in use.

4. 14. Microbial cultures should be used carefully as they are potentially pathogens.
15. Wash your hands with soap before leaving the laboratory.
16. Broth cultures should never be pipette out.
17. Label all plates, tubes, and cultures, properly before starting an experiment.
18. Materials such as stain and reagent bottles, Petri dishes, and pipettes must be
returned to their original place after use.
19. Always clean microscopes before and after use. Clean lenses with lens paper.

5. 20. Keep the laboratory doors and windows closed when the experiments are in
progress.
21. Aseptic conditions should be strictly followed at all times.
22. It is advisable to wear gloves when working with chemicals hazardous to the
eyes and skin.
23. Protect yourself from exposure of eyes and skin to UV light by wearing goggles
and clothing respectively.