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Laboratory safety
Prepared by
Samira fattah
Assis. Lec.
College of health sciences-HMU
Lab. 1
Introduction
• Laboratory safety rules are a major aspect of
every microbiology lab.
• Each student in microbiology laboratory must
follow specific safety rules and procedures.
Why is Lab Safety Important?
• Lab safety rules and
symbols are needed so
that students do not injure
themselves or their
classmates.
Lab Safety Rules
Lab Safety Rules
1-Wear protective clothing .
Protective clothes
o Gloves are essential.
o Lab coats are
required.
o Safety glasses
( goggles) may be
required to avoid
splashes.
2-Laboratory personnel should not wear sandals
Lab Safety Rules
Lab Safety Rules
Do NOT Wear:
• Jewelry
• Loose or Baggy clothing
3-Avoid touching objects (e.g., pencils, cell phones,
door handles) while wearing gloves.
Lab Safety Rules
4-Pencils, labels, or any other materials should never be
placed in your mouth.
5-Caution must be taken when using gas burners. Be
sure gas burners are turned off when finished.
6-Long hair must be tied back or covered to minimize
fire hazard or contamination of experiments.
Lab Safety Rules
Lab Safety Rules
7- Do not eat food or drink water in the lab.
do not use lab glassware as food or water containers.
Lab Safety Rules
8- Protect your hands safety:
- wash hands after every lab.
- Handle glassware, sharp tools and heated containers carefully.
Lab Safety Rules
9- electrical safety:
- unplug electrical equipment after use.
- keep all electrical cords and wires away from water .
Lab Safety Rules
10- Chemical safety:
-never touch, taste or smell a chemical unless instructed to do
so.
- never mix chemicals unless instructed to do so.
-keep lids on chemical containers when not in use.
Lab Safety Rules
11-Do not take any cultures out of the
lab for any reason.
• All cultures should be handled as
potentially pathogenic.
• Liquid cultures must always be
kept in a test tube rack.
Lab Safety Rules
12- Do not engage in practical jokes or horseplay in the
lab.
Lab Safety Rules
13-Keep nonessential books and clothing far away from
your work area.
Lab Safety Rules
14-Wipe the bench tops down with disinfectant both before you
begin your work and after you have completed your work.
Lab Safety Rules
15- Dispose of waste products according to instructions.
16-Report all accidents, no matter how minor, to
your supervisor!!
Lab Safety Rules
Lab Safety Equipment
Lab Safety Equipment
• Safety shower
• Eye wash
Lab Safety Equipment
• Fire Blanket
• Fire Extinguisher
Lab Safety Symbols
Lab Safety Symbols
• They alert about the possible dangers in the lab.
Biological Safety Levels
Biological Safety Levels
1. BSL1
2. BSL2
3. BSL3
4. BSL4
BSL-1
– Not known to cause disease in healthy
adult humans
• Safety equipment
– Minimal requirements
• Facilities
– Open bench top
BSL-2
– Associated with mild to moderate
disease in humans
• Safety equipment
– Biological Safety Cabinet and
personal protective equipment as
needed.
• Facilities
– BSL-1 plus the availability of a
mechanism for decontamination.
BSL-3
– Associated with serious or
potentially lethal disease in
humans
• Safety equipment
– Biological Safety Cabinet and
personal protective equipment
required.
• Facilities
– BSL-2 with self-closing double
door access and single-pass
negative directional airflow.
BSL-4
– Associated with high risk of
life-threatening disease in
humans and/or animals
• Safety equipment
– Biological Safety Cabinet
– Full-body air-supplied,
positive pressure personnel
suit
• Facilities
– BSL-3 plus dedicated air and
exhaust, decontamination
procedures for exit, separate
building, etc.

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