This document provides guidance on having difficult conversations with employees. It discusses 10 ways to make tough talks easier such as addressing issues as soon as possible, setting a specific time and place, focusing on the problem not the person, being collaborative, and documenting the meeting. The document also discusses how to provide feedback so it is heard, including asking permission, using a soft entry, not blaming others, keeping it simple and direct, and reaching agreement. Finally, it provides conversation starters and notes the importance of documentation.
In these 5 tips you will learn the best practice when filming a Vox Pops interview video. Discover how to get release forms, overcome rejection and setup sound for a vox pops video.
www.boldcontent.tv
phage for detection of viable Salmonella typhimuriumLiliane Majed
Development of an engineered bioluminescent reporter phage for the sensitive detection of viable Salmonella typhimurium
Because foodborne illnesses continuously threaten public health, rapid
and sensitive detection of pathogens in food has become an important issue. As an
alternative to time-consuming and laborious conventional detection methods, a
technique using recombinant reporter phages has been developed. Here, we developed
an advanced bioluminescent reporter phage SPC32H-CDABE by inserting a bacterial
luxCDABE operon into the Salmonella temperate phage SPC32H genome. Whole
SPC32H genome sequencing enabled the selection of nonessential genes, which can
be replaced with approximately 6-kb luxCDABE operon, which provides both
luciferase (LuxAB) and its substrate, fatty aldehyde, as generated by fatty acid
reductase (LuxCDE). Thus, the SPC32H-CDABE detection assay is simpler and more
efficient compared to the luxAB-based assay because the substrate addition step is
excluded. At least 20 CFU/mL of pure S. Typhimurium culture was detectable using
SPC32H-CDABE within 2 h, and the signals increased proportionally to the number
of cells contaminated in lettuce, sliced pork, and milk. These results thereby demonstrate that this phage successfully detects live
Salmonella without appreciable interference from food components. Furthermore, the presented data suggest that SPC32HCDABE
represents a promising easy-to-use diagnostic tool for the detection of Salmonella contamination in food.
Seongmi Kim,# Minsik Kim,# and Sangryeol Ryu*
Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute for Agriculture and
Life Sciences, and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, South Korea
pubmed link :
http://www.ncbi.nlm.nih.gov/pubmed/24806327
french traduction and power point presentation by Liliane M. Lebanese university - Section 1 - Hadath
Biology department
In these 5 tips you will learn the best practice when filming a Vox Pops interview video. Discover how to get release forms, overcome rejection and setup sound for a vox pops video.
www.boldcontent.tv
phage for detection of viable Salmonella typhimuriumLiliane Majed
Development of an engineered bioluminescent reporter phage for the sensitive detection of viable Salmonella typhimurium
Because foodborne illnesses continuously threaten public health, rapid
and sensitive detection of pathogens in food has become an important issue. As an
alternative to time-consuming and laborious conventional detection methods, a
technique using recombinant reporter phages has been developed. Here, we developed
an advanced bioluminescent reporter phage SPC32H-CDABE by inserting a bacterial
luxCDABE operon into the Salmonella temperate phage SPC32H genome. Whole
SPC32H genome sequencing enabled the selection of nonessential genes, which can
be replaced with approximately 6-kb luxCDABE operon, which provides both
luciferase (LuxAB) and its substrate, fatty aldehyde, as generated by fatty acid
reductase (LuxCDE). Thus, the SPC32H-CDABE detection assay is simpler and more
efficient compared to the luxAB-based assay because the substrate addition step is
excluded. At least 20 CFU/mL of pure S. Typhimurium culture was detectable using
SPC32H-CDABE within 2 h, and the signals increased proportionally to the number
of cells contaminated in lettuce, sliced pork, and milk. These results thereby demonstrate that this phage successfully detects live
Salmonella without appreciable interference from food components. Furthermore, the presented data suggest that SPC32HCDABE
represents a promising easy-to-use diagnostic tool for the detection of Salmonella contamination in food.
Seongmi Kim,# Minsik Kim,# and Sangryeol Ryu*
Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute for Agriculture and
Life Sciences, and Center for Food and Bioconvergence, Seoul National University, Seoul 151-921, South Korea
pubmed link :
http://www.ncbi.nlm.nih.gov/pubmed/24806327
french traduction and power point presentation by Liliane M. Lebanese university - Section 1 - Hadath
Biology department
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Want your customer service representatives to deliver great customer service? Help them become customer service superstars. Simple things you can do to bring out the superstar in your customer service delivering employees.
How to prepare for an interview
Attending an interview is not an easy one as one would be meeting people who are not known and will have to introduce themselves to others. Proper preparation helps to reduce the stress level and let you speak more confidently. Practicing before the interview boosts your confidence level. Apt dressing is an important and most crucial factor for attending an interview. Having multiple copies of resumes along with a pen and paper for making notes let you make a note of possible mistakes that can occur which will help you prepare better in the future. Always relax and stay calm while attending and listen carefully to the questions being asked so as to give a proper answer. Relating known facts along with facts about the company helps you to make a match between your expertise and company’s requirements. Make a follow up with a thank you note to reiterate your interest in the position.
1-ten commandments of positive relationships.
2-Communication barriers.
3-body language.
3- Listening skills
4-Johari window .
5-presentation skills.
6-email-writing-skills
1-speak to people.
2-smile at people.
3-call people by name.
4-be friendly and helpful.
5-be cordial.
6-be interested.
7-be generous .
8-be considerate.
9-be alert to give service.
10-add to this good sense of humor
Listening skills
Body language
Johari window
six thing people determine fram your appearance
presentation skills
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How to keep safety top-of-mind.
Making safety a responsibility for all employees.
Creating a line of communication about safety issues.
Implementing an effective safety program.
How to maintain organize and consistent safety practices.
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Want your customer service representatives to deliver great customer service? Help them become customer service superstars. Simple things you can do to bring out the superstar in your customer service delivering employees.
How to prepare for an interview
Attending an interview is not an easy one as one would be meeting people who are not known and will have to introduce themselves to others. Proper preparation helps to reduce the stress level and let you speak more confidently. Practicing before the interview boosts your confidence level. Apt dressing is an important and most crucial factor for attending an interview. Having multiple copies of resumes along with a pen and paper for making notes let you make a note of possible mistakes that can occur which will help you prepare better in the future. Always relax and stay calm while attending and listen carefully to the questions being asked so as to give a proper answer. Relating known facts along with facts about the company helps you to make a match between your expertise and company’s requirements. Make a follow up with a thank you note to reiterate your interest in the position.
1-ten commandments of positive relationships.
2-Communication barriers.
3-body language.
3- Listening skills
4-Johari window .
5-presentation skills.
6-email-writing-skills
1-speak to people.
2-smile at people.
3-call people by name.
4-be friendly and helpful.
5-be cordial.
6-be interested.
7-be generous .
8-be considerate.
9-be alert to give service.
10-add to this good sense of humor
Listening skills
Body language
Johari window
six thing people determine fram your appearance
presentation skills
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With all of the regulations and forms, FMLA can be very confusing to understand and comply with. The overlapping State and Federal laws, eligibility qualifications, and process requirements make FMLA into a complicated regulation, but with the right information it doesn’t have to be a headache. Join KPA’s Human Resource Advocate, Kim Kavanagh, as she helps to take the mystery out of FMLA. Kim will delve into:
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How to maintain organize and consistent safety practices.
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Hard Conversations: How to have tough talks with employees
1. – KPA CONFIDENTIAL –
Hard Conversations: How to have
tough talks with employees
February 4, 2010
2. Learning Objectives
• 10 ways to make a tough talk
easier
• How to provide feedback so it
is heard
• Conversation starters
• Documentation
2– KPA CONFIDENTIAL –
4. Start With Yourself
• What is the purpose of the conversation?
• What do you want to accomplish?
• What assumptions are you making about
the person’s intentions?
• How is your attitude toward the
conversation influencing your behavior?
• What are the facts about the situation?
4– KPA CONFIDENTIAL –
5. 10 Ways To Make A Tough Talk Easier
1. Address the problem as soon as possible
2. Set a specific time and place
3. Sit, don’t stand
4. Private location
5. Focus on the issue, not the person
6. Be specific about the concern
7. Be collaborative
8. Avoid negative statements
9. Encourage the subordinate
10.Document the meeting
5– KPA CONFIDENTIAL –
6. Making Yourself Heard
• Ask permission to provide the feedback
– “ I have something I need to discuss with you, when are
you available?”
• Use a soft entry
– “ I have some feedback that is rather difficult to hear”
• Don’t blame others
– “this is behavior that I (or company policy) require you
to change”
• Keep it simple and be direct
– “I am talking with you because this is an issue you need
to address”
• Accentuate the positive
– “If you can resolve this issue we can discuss the
promotion
6–
7. Making Yourself Heard
• Be sensitive to culture differences
– “I understand that this might be new information for
you”
• Reach agreement about the required change
– “So you will set your alarm 10 minutes earlier to get
here on time?”
• Confirm the dates
– “We will see this change by tomorrow and then
ongoing”
• Follow- Up
– “Let’s get together next Thursday to review progress”
7– KPA CONFIDENTIAL –
8. Three Of The Toughest Talks
• Personal Hygiene
– Be sensitive to cultural difference
– Tie to business concerns
– Involve medical personal if not able to resolve
• Poor Performance
– Specific time, date, individual involved
– Do not make it personal
– Document the required change
• No Raise
– Review performance and offer praise
– Explain the business circumstance
– Be consistent and be fair
8– KPA CONFIDENTIAL –
9. Conversation Starters
• “ I have something I would like to discuss
with you that will help us work together
more effectively”
• “I’d like to talk with you about___, but first I
would like to get your point of view”
• “I need your help with what just happened,
do you have a few minutes to talk about it”
• “This is a hard thing to hear, and a hard
thing for me to tell you but we need to
resolve as quickly as possible”
9– KPA CONFIDENTIAL –
10. Documentation
• Always document the conversation
– Notes if a feedback session or oral warning
• Time, location, attending, issue, resolution, follow-up
– Disciplinary Action Form if a serious offense
10– KPA CONFIDENTIAL –
11. Keep Calm and Carry On
• Prepare, use a script if necessary
• Select time and location
• Be objective, never make it personal
• Remain calm at all time
• Address as quickly as possible, don’t delay
it won’t go away
• Document all conversations
• Set time to follow up- positive or negative
reinforcement
11– KPA CONFIDENTIAL –