The document discusses various sperm preparation methods used in assisted reproductive technology (ART) procedures, specifically comparing IVF and IUI techniques. It details methods such as simple sperm wash, swim up technique, and density gradient sperm wash, each varying in complexity and effectiveness. The sperm preparation techniques aim to enhance the viability of sperm used in IUI by separating healthy sperm from less active or dead cells.

2. ART procedure : IVF vs IUI

3. ART procedure ICSI

4. ART procedure PICSI

5. DIFFERENT SPERM
PREPARATION METHOD
• Simple Sperm Wash
• Semen is diluted in a test tube with a special
solution of antibiotics and protein
supplements. It is then placed in a centrifuge,
a machine that spins around at extremely
high speeds.As the sperm mixture is spun,
sperm cells fall to the bottom of the test
tube, producing a mass of dense, highly active
sperm.These sperm can then be removed
from the test tube and used in IUI.A simple
sperm wash takes about 20 to 40 minutes.

6. DIFFERENT SPERM PREPARATION METHOD
• A. SWIM UP: The swim up technique is rapidly becoming more
and more popular throughout American fertility clinics. semen
is placed in a culture dish with a layer of media culture. Sperm
are attracted to this culture, and will swim up to it. As the
sperm swim up to the culture, they are collected. This
technique takes about two hours in order to harvest enough
sperm for use in IUI. Only the most powerful sperm will be
able to do this. In order to retrieve this powerful sperm.
• This technique revolves around the fact that sperm need to
swim forwards and up in order to
reach the uterus.

7. • Density Gradient Sperm Wash
• The density gradient sperm wash is one of the most popular sperm washing methods. This is
because it also works to separate dead sperm cells, white blood cells, and other waste
products from the sperm.
• A test tube is filled with multiple layers of liquids of different densities. Semen is then placed on
the top layer of liquid and the test tube is spun in a centrifuge. After it is spun, active, healthy
sperm will make their way to the very bottom layer of liquid in the test tube, while debris and
dead sperm will get caught in the top two layers. These layers can be siphoned off in order to
remove the active sperm from the test tube. This sperm is then used in the IUI procedure.
• Density gradient sperm washes take approximately 60 minutes.
DIFFERENT SPERM PREPARATION METHOD