This document discusses genomic research technologies, including the invader assay. The invader assay is used to detect single nucleotide polymorphisms without requiring PCR. It works by using reporter and invader probes that are cleaved by a flap endonuclease only when the target sequence is present. The assay has the advantage of not needing PCR but has drawbacks of requiring a large sample size and having background reactions. Overall, the invader assay is a method for detecting SNPs and mutations without PCR amplification.