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1. INTRODUCTION
TO HISTOLOGICAL
TECHNIQUES
B Y A A M I R R A S H I D K H A N
C L I N I C A L T U T O R
H I S T O L O G Y L A B
O C H S - M U S C A T

2. DEFINITIONS-
• Histological techniques are a number of steps which is taken by Histotechnologist in
order to prepare biopsy sample for microscopic examination in a histology lab.
• Biopsy
• Histology Lab ( Histopathology Lab)
• HistoTechnician/Technologist

3. COMMON STEPS DURING
PROCESSING-
• Biopsy procedure
• Initial fixation
• Registration
• Grossing
• Completion of fixation

4. CONT.
• Processing (Manual/ Automated)
Completion of Fixation*
Dehydration
Clearing
Impregnation

5. EMBEDDING
• Block making
• Use of paraffin
• Use of machines
• Cassettes and moulds

6. MICROTOMY
• AKA section cutting
• Microtome
• Ribbon
• Thickness
• Knife/ Blades

7. STAINING AND MOUNTING
• Universal stain H&E
• Types of staining solutions / reagents used
• Progressive and Regressive
• Use of automation

8. SUBMISSION AND REPORTING
• Final submission
• QC check before submission
• Reporting by a Histopathologist only!

9. • Thank you.