The document provides instructions for using a Polax 2L polarimeter to measure specific rotation of samples. Key steps include: setting the front panel display to 0.00, rinsing and filling the observation tube with sample solution, checking for bubbles, placing the tube in the polarimeter and adjusting brightness of fields, recording the angle of rotation and calculating specific rotation using a provided formula.

1. PETUNJUK PENGGUNAAN
POLARIMETER POLAX 2L (ATAGO)
Specific Rotation Measurements
• Press and hold both the “TEMP” button and either the “R + ” rotate right
handed.
• button or the “L – ” rotate left handed button to set the front panel display to
“0.00”
• Open sample chamber cover and remove observation tube.
• Rinse observation tube three times with sample solution to be tested,
overfill tube with sample solution until it forms a meniscus. Note: pour
slowly to avoid formation of bubbles.
• Turn observation tube upside down and tap tube gently to observe any
bubbles. For large bubbles, observation tube should be re-filled with
sample solution to be tested. Place filled observation tube in polarimeter
and close sample chamber cover
• If the right semicircular field is brighter, then press and hold both the
“TEMP” button and the “R + ” rotate right handed button to approximately
equalize the brightness of the right and left translucent semicircular fields
while observing the fields through the eyepiece
• If the left semicircular field is brighter, then press and hold both the
“TEMP” button and the “L – ” rotate left handed button to approximately
equalize the brightness of the right and left translucent semicircular fields
while observing the fields through the eyepiece
• Press the the “R + ” rotate right handed button or the “L – ” rotate left handed
button to fine adjust the brightness of the right and left translucent
semicircular fields while observing the fields through the eyepiece
• Record angle of rotation displayed on front panel and calculate specific
rotation using following formula:
“a” is angle of rotation
“l” is length of observation tube in decimeters ( 2 dm)
“c” is grams of sample per 100 ml
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