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Milk Ketone Bodies Assessment in A Local Italian Cow Breed (Modenese) vs. Hol...Agriculture Journal IJOEAR
Abstract— In Northern Italy are present several autochthonous cattle breeds characterized by a small territorial diffusion. These breeds were breed for century the milk and meat production but also for work. The technical data show that these animals have a good reproductive performances and disease resistance and resilience. The objective of this study was to characterize some productive, reproductive and metabolic parameters (ketone bodies) in the Italian autochthonous cattle breeds Modenese, comparing them with those of Holstein and crossbred (F1=Modenese x Holstein; F2=Modenese x F1) breed in the same farm in order to understand if there is a different metabolic situation that can influence the reproductive performances. The milk samples have been taken at different times of lactation. Milk samples have been collected from the whole udder during morning milking and from a lactometer and analyzed by Gas chromatography-mass spectrometry to obtain the ketone bodies concentration. The reproductive (Open Days Period and number of Services Per Pregnancy) and productive (percentage and kg of protein between the 40th and 90th DIM) data have been recovered by the consultation of the farm registers and the APA (Provincial breeder association) data. On days open, number of services per pregnancy, % of proteins in milk, and kg of proteins in milk, a Spearman correlation analysis was applied. The comparative study between the races in the same breeding conditions is a fundamental tool to understand the correct reproductive and productive physiology of dairy cattle. Among the ketone bodies concentration, the Modenese breed showed a significant (p<0,05) lower concentration. A high concentration of ketone bodies is related with serious negative energy balance condition that can affect the reproductive performances. The F1, F2 and Modenese showed also better reproductive performances compared to Holstein, with a day’s open length in average between the 80-105 days. In conclusion, the better resilience against the negative energy balance and his adverse effects of Modenese cattle could be one of the phenomena underlying their better reproductive efficiency.
Austin publishing group - Oral kefir grains supplementation improves metaboli...Austin Publishing Group
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Background: Foodborne infections like Listeriosis cover several disorders and are a worldwide public health emergency. Listeria monocytogenes has been isolated from various foodstuffs, including milk and fishes. MALDI-TOF mass spectrometry technique is known for its rapid and accurate identification of bacterial organisms.
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Result: In current study, on screening 360 animal origin food samples, three isolates were recovered from raw milk samples and identified as Listeria monocytogenes indicating an overall prevalence of Listeria monocytogenes to the tune of 0.83%. Excellent correlation was observed with identification of Listeria species using conventional phenotypic tests and advanced molecular tool Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) technique. The results depict dependability of advanced technique for rapid and reliable identification of Listeria species.
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Identification of functional novel traits in dairy cattle.pptx
1. IDENTIFICATION OF FUNCTIONAL NOVEL TRAITS
IN DAIRY CATTLE
Novel traits?
Need? Identification?
Limitation?
Dr. Sakshi Vaishnav
(PhD Scholar, IVRI)
2. A trait is defined as novel if a heritable change in that trait allows for the exploitation of a new
behavioural or ecological niche or allows population persistence in the face of a challenge that the
population had not previously encountered.
DEFINITION
Word of origin : Latin word novellus meaning “NEW”
Traits that are being introduced to breeding goals in order to satisfy new demands faced by livestock
production or trait that has not previously been widely recorded but are economically, environmentally
or societally important.
4. NEED OF NOVEL TRAITS?
World population: 9 billion in 2050 (UN reports)
Increase in food production, demand for limited resources
Change in global environment
Change in breeding goal with greater emphasis on novel traits
5. DIFFERENT TYPES OF TRAITS
Functional traits are morphological, biochemical, physiological, structural or behavioural
characteristics that are expressed in phenotypes of individual organism and considered relevant
to response of such organism and/or their effect on ecosystem properties.
Or
Traits that are not directly linked to production and reproduction but indirectly influence them
greatly by playing major role in fitness.
1. Reproduction traits
2. Production traits
3. Functional traits
6. NOVEL TRAITS IN DAIRY CATTLE
• Current breeding goal: Genetic improvement of production traits
• Functional traits- negative correlation- production traits
• Compensation: Identification and selection of novel traits
Traits may be novel in one country and existing in others.
Identification of novel traits
Recording programmes
Use of advanced technologies ( SNP based genotyping, sequencing, metabolomics, proteomics)
7. 1. Direct health traits
• Recording- Treatment , diagnosis
• Data standardisation
• Routine genetic evaluation
• Collaboration and combination of data coverage of trait
Genomic evaluation – Jersey cattle (ADSA)
Traits measured – milk fever, displaced abomasum, ketosis, mastitis, metritis and retained
placenta.
Genetic evaluation
8. • Mastitis diagnosis, early reproductive disorders, cystic ovaries and milk fever- Austria
• Two new indices:
1. FRW (fertility index) – EREP and CYST
2. EGW (Udder health index)- SCC (70%) and MAS(30%) Udder confirmation traits
C. Fuerst & C. Egger-Danner (2016)
9. 2. Feet and legs traits
• Third reason of culling
• Indicator trait for claw health
• Confirmation recording: Linear type classification
Locomotion scoring
Lameness scoring
Automated lameness detection- activity sensors
• Structural soundness – beef industry – longevity
and welfare
• Enough measurements – genetic evaluation
Rowan(2021)
10. 3. Metabolic status
• Direct traits rarely used
• Milk content traits – early diagnosis
Limitations - limited predictive ability
Solution- automated milk recording system
• Subclinical metabolic disorders- ketosis- Non
esterified fatty acids or BHBA concentration
• Body condition score
• Rumen activity
• Body weight change
• Identification of important metabolites
11. 4. Climate resilience
• Heat stress : Impaired welfare production losses
• Selection for heat stress:
Impact of heat stress in population (PLF technologies)
Define and measure heat stress (THI, MIR-predicted fatty acid C18:1 cis 9 content in milk to heat stress,
heat shock proteins )
Ways to model heat stress
Identification of heat tolerant animals
5. Behavioural traits – subjectively scored
Temperament
Milking speed: Slow production losses ; Fast Mastitis (optimum: 2 Kg/min)
Suckling behaviours
12. LIMITATIONS OF NOVEL TRAITS
• Difficult to measure
• Laborious and expensive recording
• Negative correlation with production traits
• Less number of observations available
• Most of the novel traits have low heritability and hence difficult to improve by conventional methods