This document discusses value-based purchasing and pay-for-performance programs implemented by the Centers for Medicare and Medicaid Services (CMS). It provides an overview of key CMS value-based programs for hospitals, home health agencies, skilled nursing facilities, end-stage renal disease facilities, and physicians. The goals are to improve quality of care, patient outcomes, and reduce healthcare costs through linking provider payments to performance and quality measures. The document describes the measures, payment adjustments, and potential incentives/penalties associated with each program.
The document discusses components of the revenue cycle in healthcare, including pre-claims submission, claims processing, accounts receivable, claims reconciliation, and collections. It describes the charge description master which houses billing information for healthcare services and supplies. Effective revenue cycle management is important for a provider's financial stability. The revenue cycle involves coordination between many departments to accurately capture and bill for services provided to patients.
The resource-based relative value scale (RBRVS) is used to determine reimbursement for professional services under Medicare Part B. RBRVS assigns relative value units (RVUs) to codes based on physician work, practice expense, and malpractice insurance. RVUs are adjusted by geographic practice cost indexes and multiplied by a conversion factor to calculate payment amounts. Correct coding and documentation are essential for full and accurate RBRVS reimbursement. Unnecessary administrative costs associated with health plan management can significantly impact provider revenues.
MIPS APM for ACOs: A Hybrid Reimbursement ModelCitiusTech
This document discusses MIPS APM scoring for ACOs that do not meet the patient and payment thresholds to be classified as Advanced APMs. It provides an overview of MIPS APM reporting requirements and timelines, the measures ACOs can report through various methods like surveys and claims, and how payment adjustments will be determined based on a composite performance score. Key advantages of MIPS APM scoring include reduced reporting burdens and greater weight given to quality over cost measures.
This document discusses Medicare's inpatient prospective payment systems (IPPS) for reimbursing hospitals. It defines key concepts like major diagnostic categories (MDCs), diagnosis-related groups (DRGs), and Medicare severity diagnosis-related groups (MS-DRGs). It explains the multi-step process for assigning a case to an MS-DRG based on principal diagnosis, procedures, complications/comorbidities, and other criteria. The goal of IPPS is to prospectively pay hospitals a pre-determined rate based on the expected costs for treating a patient in a particular MS-DRG.
This document discusses Medicare's prospective payment system for home health agencies. It provides key details about the home health prospective payment system including: how agencies are reimbursed using a case rate methodology based on 60-day episodes of care; how data is collected using OASIS assessments and ICD codes; how payments are determined based on home health resource groups which classify patients into clinical severity, functional status and service utilization categories; and how the standardized episode rate is calculated and adjusted based on wage indexes and other payment provisions like LUPAs.
This document discusses Medicare's prospective payment system for long-term care hospitals (LTCHs). It describes how LTCHs are reimbursed under Medicare Part A based on Medicare-severity long-term care diagnosis-related groups (MS-LTC-DRGs). Admissions are grouped into MS-LTC-DRGs which are assigned relative weights based on expected resource use. Payments are calculated by multiplying a standardized federal base rate by the MS-LTC-DRG relative weight. The base rate is adjusted for area wage levels and provisions exist for short stays, interrupted stays, high cost outliers, and restrictions on "hospitals within hospitals." Data is collected using the LTCH Continuity Assessment Record and Evaluation
This document discusses Medicare's prospective payment system for inpatient rehabilitation facilities (IRFs). It provides an overview of IRFs, including the services they provide and requirements for reimbursement. The key aspects of the IRF prospective payment system are described, including the case rate methodology, compliance percentage, data collection via the Patient Assessment Instrument, case mix group structure, and provisions for additional payments in certain situations. Classification of patients into case mix groups determines the reimbursement amount an IRF receives.
This document discusses value-based purchasing and pay-for-performance programs implemented by the Centers for Medicare and Medicaid Services (CMS). It provides an overview of key CMS value-based programs for hospitals, home health agencies, skilled nursing facilities, end-stage renal disease facilities, and physicians. The goals are to improve quality of care, patient outcomes, and reduce healthcare costs through linking provider payments to performance and quality measures. The document describes the measures, payment adjustments, and potential incentives/penalties associated with each program.
The document discusses components of the revenue cycle in healthcare, including pre-claims submission, claims processing, accounts receivable, claims reconciliation, and collections. It describes the charge description master which houses billing information for healthcare services and supplies. Effective revenue cycle management is important for a provider's financial stability. The revenue cycle involves coordination between many departments to accurately capture and bill for services provided to patients.
The resource-based relative value scale (RBRVS) is used to determine reimbursement for professional services under Medicare Part B. RBRVS assigns relative value units (RVUs) to codes based on physician work, practice expense, and malpractice insurance. RVUs are adjusted by geographic practice cost indexes and multiplied by a conversion factor to calculate payment amounts. Correct coding and documentation are essential for full and accurate RBRVS reimbursement. Unnecessary administrative costs associated with health plan management can significantly impact provider revenues.
MIPS APM for ACOs: A Hybrid Reimbursement ModelCitiusTech
This document discusses MIPS APM scoring for ACOs that do not meet the patient and payment thresholds to be classified as Advanced APMs. It provides an overview of MIPS APM reporting requirements and timelines, the measures ACOs can report through various methods like surveys and claims, and how payment adjustments will be determined based on a composite performance score. Key advantages of MIPS APM scoring include reduced reporting burdens and greater weight given to quality over cost measures.
This document discusses Medicare's inpatient prospective payment systems (IPPS) for reimbursing hospitals. It defines key concepts like major diagnostic categories (MDCs), diagnosis-related groups (DRGs), and Medicare severity diagnosis-related groups (MS-DRGs). It explains the multi-step process for assigning a case to an MS-DRG based on principal diagnosis, procedures, complications/comorbidities, and other criteria. The goal of IPPS is to prospectively pay hospitals a pre-determined rate based on the expected costs for treating a patient in a particular MS-DRG.
This document discusses Medicare's prospective payment system for home health agencies. It provides key details about the home health prospective payment system including: how agencies are reimbursed using a case rate methodology based on 60-day episodes of care; how data is collected using OASIS assessments and ICD codes; how payments are determined based on home health resource groups which classify patients into clinical severity, functional status and service utilization categories; and how the standardized episode rate is calculated and adjusted based on wage indexes and other payment provisions like LUPAs.
This document discusses Medicare's prospective payment system for long-term care hospitals (LTCHs). It describes how LTCHs are reimbursed under Medicare Part A based on Medicare-severity long-term care diagnosis-related groups (MS-LTC-DRGs). Admissions are grouped into MS-LTC-DRGs which are assigned relative weights based on expected resource use. Payments are calculated by multiplying a standardized federal base rate by the MS-LTC-DRG relative weight. The base rate is adjusted for area wage levels and provisions exist for short stays, interrupted stays, high cost outliers, and restrictions on "hospitals within hospitals." Data is collected using the LTCH Continuity Assessment Record and Evaluation
This document discusses Medicare's prospective payment system for inpatient rehabilitation facilities (IRFs). It provides an overview of IRFs, including the services they provide and requirements for reimbursement. The key aspects of the IRF prospective payment system are described, including the case rate methodology, compliance percentage, data collection via the Patient Assessment Instrument, case mix group structure, and provisions for additional payments in certain situations. Classification of patients into case mix groups determines the reimbursement amount an IRF receives.
As healthcare is a language “all its own,” PYA Principal David McMillan presented “Learning the New Language of Healthcare" at the Georgia Society of CPAs' 2014 Healthcare Conference.
This chapter discusses Medicare's prospective payment systems for post-acute care settings like skilled nursing facilities. It describes how Medicare uses a per diem payment model and adjusts payments based on a facility's costs and a patient's care needs, which are assessed using the Minimum Data Set and classified into Resource Utilization Groups. Facilities are reimbursed using an adjusted per diem rate based on the patient's RUG classification and length of stay. The chapter also explains how these payment systems aim to ensure beneficiaries receive appropriate high-quality care in the least costly post-acute care setting.
ICD-10 Is Really Here: What Does That Mean To Compliance Officers?PYA, P.C.
This document provides an overview of a presentation for the Tennessee Hospital Association's 2015 Fall Compliance Conference on ICD-10 implementation. The presentation covers the current regulatory status of ICD-10, an overview of industry testing successes and challenges, how ICD-10 will be used for outcome-based and population health data in the future, and what to expect regarding claim denials. It also discusses bills in Congress regarding ICD-10 transition and provides examples of Medicare coverage determination changes.
ICD-10 Transition Presentation: What Health Lawyers Need to KnowPYA, P.C.
PYA Consulting Principal Denise Hall, along with co-presenter Julie Chicoine, recently updated health lawyers about ICD-10 transition readiness at the American Health Lawyers Association Institute on Medicare and Medicaid Payment Issues, held March 26-27, 2014.
Quality and Outcome Framework (QOF) is a voluntary annual incentive programme for GPs in England, detailing practice achievement results. The primary objective of QOF is to drive the quality of primary care and reduce variations in the quality of care amongst GPs
ICD-10 Transition: What Health Lawyers Need to KnowPYA, P.C.
PYA Principal Denise Hall, along with Senior Corporate Counsel Julie Chicoine of Ohio State University Wexner Medical Center, presented “ICD-10 Transition: What Health Lawyers Need to Know” at the AHLA 2015 Institute on Medicare and Medicaid Payment Issues.
Speaking before the Georgia Pediatric Practice Managers Association, PYA Consultant and ICD-10-CM Trainer Kim-Marie Walker addressed recent ICD-10 developments along with basic guidance for the transition, including:
• Comparison of ICD-9 and ICD-10
• ICD-10 organizational and structural differences
• Vendor recommendations and available resources
• Transition planning and roles
Modern Physician-Hospital Affiliations in an Era of Increased Fraud and Abuse...PYA, P.C.
PYA Senior Manager Chris Beckham co-presented “Modern Physician-Hospital Affiliations in an Era of Increased Fraud and Abuse Scrutiny” with Ross Burris of Polsinelli at the American Health Lawyer Association’s (AHLA) Physicians and Hospitals Law Institute, February 8-10, 2016.
The Uncertain Future of Medicare Add-Ons and Pass-ThroughsBESLER
With so many changes resulting from the Patient Protection and Affordable Care Act (ACA) and other potential initiatives under consideration, a significant amount of your organization’s future Medicare revenue may be at risk. The trend to reduce and/or revamp payment methodologies comes at a time when hospitals face shrinking or non-existent margins. Revenue sources potentially on the chopping block include Medicare Bad Debt, Nursing Allied Health, Graduate Medical Education, Wage Index adjustments, and transplant, to name a few. Additionally, the Office of Inspector General (OIG) continues to add reimbursement-related topics to its annual Work Plan, expanding the areas for potential paybacks or penalties.
CMS’ Hospital Readmission Reduction Program: What does it mean for your hospi...PYA, P.C.
The document summarizes information presented at an Alabama Hospital Association meeting about the CMS Hospital Readmission Reduction Program. It discusses rising healthcare costs, the shift from fee-for-service to value-based reimbursement, and new programs linking hospital payment to quality metrics like readmission rates. Hospitals face reductions of up to 3% of Medicare reimbursement payments if they have excess readmissions for conditions like heart attacks, heart failure and pneumonia. The presentation provides Alabama-specific data on the financial impacts of readmission adjustments and new billing codes for transitional care management.
Uncertain future of medicare pass throughs and add-onsBESLER
Very few items are still settled on your cost report. With so many changes resulting from the ACA and other potential initiatives being discussed every day, your organization should be acutely aware of the total amount of Medicare Revenue that is at risk. There is talk of eliminating, greatly reducing or completely altering payment methodologies that hospitals have become so reliant on for so long. Revenue potentially at risk includes Medicare Bad Debt, Nursing Allied Health, Graduate Medical Education, Wage Index adjustments, and Transplant.
The Evolving Role of the Compliance Officer in the Age of Accountable CarePYA, P.C.
Much has been written about new competencies physicians must develop in the face of payment and delivery system reform. But providers are not the only ones seeing their roles change. Compliance officers, who serve as organizations’ internal police officers, will have many new challenges. PYA Principal Martie Ross presented a national Health Care Compliance Association (HCCA) webinar entitled “The Evolving Role of the Compliance Officer In the Age of Accountable Care.”
The Heartaches Associated with Billing for Cardiac DevicesPYA, P.C.
PYA Principal Denise Hall-Gaulin and Consulting Manager Joanna Malcolm presented a free webinar for the Georgia chapter of the Healthcare Financial Management Association, on Tuesday, December 6, 2016.
The presentation was geared toward C-suite hospital leaders, compliance officers, in-house counsel, operational leaders, and patient accounting leadership, and covered:
The criteria for implantable cardioverter defibrillators (ICDs), pacemakers, and other devices
The documentation requirements for payment
The prerequisites for a clean audit
Accounting Update Overview with a Healthcare SlantPYA, P.C.
PYA Principal and Director of Audit Services Doug Arnold presented during East Tennessee State University’s 38th Annual Accounting, Auditing, and Tax Updating CPE conference. His presentation covered many recent Accounting Standards Updates, but leaned toward their applications in healthcare.
This document outlines the 10 steps of the medical billing cycle: 1) preregistering patients, 2) establishing financial responsibility, 3) checking patients in, 4) checking patients out and documenting diagnoses and procedures, 5) reviewing coding for compliance, 6) checking billing compliance, 7) preparing and transmitting insurance claims, 8) monitoring payer adjudication of claims, 9) generating patient statements, and 10) following up on payments and handling collections. It also defines key terms related to medical billing and coding.
Presentation Explores Many Contexts of Community BenefitPYA, P.C.
PYA Principal David McMillan gets to the bottom of the definition of community benefit in “Community Benefit: One Term, Many Contexts,” a presentation given at the 2013 AICPA Healthcare Industry Conference.
Presentation Uncovers Trends in the Unpredictable Healthcare IndustryPYA, P.C.
With the healthcare industry in a state of flux, not much is known about what lies ahead; but trends across the industry have become apparent and are likely to stick. These trends were the subject of a presentation given by PYA Principal David McMillan at the PKF North America Healthcare Fly-In.
PYA Healthcare Consulting Senior Manager Robert Mundy co-presented during, “Valuing Hospitals,” Thursday, July 31, at 1 p.m. EST. This webinar explores the changing world of hospital economics, regulations, and valuations and how appraisers can best prepare themselves for both the opportunities and challenges that lie ahead.
PYA Highlights Next Steps of Meaningful UsePYA, P.C.
At the 2013 AICPA Healthcare Industry Conference, PYA Principal David McMillan and Senior Manager Chris Wilson recently explored the “new normal” of meaningful use as compliance and strategic standards in new care/reimbursement-model development.
The document discusses the drivers and pressures for organizational change. It identifies that change comes from both external environmental pressures such as competition, regulations and technological changes as well as internal pressures like growth, leadership changes, and politics. Some of the key external pressures mentioned are globalization, hypercompetition, and reputation concerns. The document also examines why organizations may not change in response to environmental pressures or after crises, citing factors such as organizational learning difficulties and defensive priorities over innovation.
This document discusses evolutionary developmental biology and how changes in development can lead to evolutionary changes. It provides examples of modularity and molecular parsimony which help explain this. Modularity means parts of the body and DNA can develop differently. Molecular parsimony means organisms share developmental toolkit genes. The document then discusses specific examples like stickleback fish pelvic spines being due to different Pitx1 expression, and Darwin's finches having beak shape variations due to differing Bmp4 and Calmodulin expression levels. Mechanisms of evolutionary change include changes in location, timing, amount, or kind of gene expression.
As healthcare is a language “all its own,” PYA Principal David McMillan presented “Learning the New Language of Healthcare" at the Georgia Society of CPAs' 2014 Healthcare Conference.
This chapter discusses Medicare's prospective payment systems for post-acute care settings like skilled nursing facilities. It describes how Medicare uses a per diem payment model and adjusts payments based on a facility's costs and a patient's care needs, which are assessed using the Minimum Data Set and classified into Resource Utilization Groups. Facilities are reimbursed using an adjusted per diem rate based on the patient's RUG classification and length of stay. The chapter also explains how these payment systems aim to ensure beneficiaries receive appropriate high-quality care in the least costly post-acute care setting.
ICD-10 Is Really Here: What Does That Mean To Compliance Officers?PYA, P.C.
This document provides an overview of a presentation for the Tennessee Hospital Association's 2015 Fall Compliance Conference on ICD-10 implementation. The presentation covers the current regulatory status of ICD-10, an overview of industry testing successes and challenges, how ICD-10 will be used for outcome-based and population health data in the future, and what to expect regarding claim denials. It also discusses bills in Congress regarding ICD-10 transition and provides examples of Medicare coverage determination changes.
ICD-10 Transition Presentation: What Health Lawyers Need to KnowPYA, P.C.
PYA Consulting Principal Denise Hall, along with co-presenter Julie Chicoine, recently updated health lawyers about ICD-10 transition readiness at the American Health Lawyers Association Institute on Medicare and Medicaid Payment Issues, held March 26-27, 2014.
Quality and Outcome Framework (QOF) is a voluntary annual incentive programme for GPs in England, detailing practice achievement results. The primary objective of QOF is to drive the quality of primary care and reduce variations in the quality of care amongst GPs
ICD-10 Transition: What Health Lawyers Need to KnowPYA, P.C.
PYA Principal Denise Hall, along with Senior Corporate Counsel Julie Chicoine of Ohio State University Wexner Medical Center, presented “ICD-10 Transition: What Health Lawyers Need to Know” at the AHLA 2015 Institute on Medicare and Medicaid Payment Issues.
Speaking before the Georgia Pediatric Practice Managers Association, PYA Consultant and ICD-10-CM Trainer Kim-Marie Walker addressed recent ICD-10 developments along with basic guidance for the transition, including:
• Comparison of ICD-9 and ICD-10
• ICD-10 organizational and structural differences
• Vendor recommendations and available resources
• Transition planning and roles
Modern Physician-Hospital Affiliations in an Era of Increased Fraud and Abuse...PYA, P.C.
PYA Senior Manager Chris Beckham co-presented “Modern Physician-Hospital Affiliations in an Era of Increased Fraud and Abuse Scrutiny” with Ross Burris of Polsinelli at the American Health Lawyer Association’s (AHLA) Physicians and Hospitals Law Institute, February 8-10, 2016.
The Uncertain Future of Medicare Add-Ons and Pass-ThroughsBESLER
With so many changes resulting from the Patient Protection and Affordable Care Act (ACA) and other potential initiatives under consideration, a significant amount of your organization’s future Medicare revenue may be at risk. The trend to reduce and/or revamp payment methodologies comes at a time when hospitals face shrinking or non-existent margins. Revenue sources potentially on the chopping block include Medicare Bad Debt, Nursing Allied Health, Graduate Medical Education, Wage Index adjustments, and transplant, to name a few. Additionally, the Office of Inspector General (OIG) continues to add reimbursement-related topics to its annual Work Plan, expanding the areas for potential paybacks or penalties.
CMS’ Hospital Readmission Reduction Program: What does it mean for your hospi...PYA, P.C.
The document summarizes information presented at an Alabama Hospital Association meeting about the CMS Hospital Readmission Reduction Program. It discusses rising healthcare costs, the shift from fee-for-service to value-based reimbursement, and new programs linking hospital payment to quality metrics like readmission rates. Hospitals face reductions of up to 3% of Medicare reimbursement payments if they have excess readmissions for conditions like heart attacks, heart failure and pneumonia. The presentation provides Alabama-specific data on the financial impacts of readmission adjustments and new billing codes for transitional care management.
Uncertain future of medicare pass throughs and add-onsBESLER
Very few items are still settled on your cost report. With so many changes resulting from the ACA and other potential initiatives being discussed every day, your organization should be acutely aware of the total amount of Medicare Revenue that is at risk. There is talk of eliminating, greatly reducing or completely altering payment methodologies that hospitals have become so reliant on for so long. Revenue potentially at risk includes Medicare Bad Debt, Nursing Allied Health, Graduate Medical Education, Wage Index adjustments, and Transplant.
The Evolving Role of the Compliance Officer in the Age of Accountable CarePYA, P.C.
Much has been written about new competencies physicians must develop in the face of payment and delivery system reform. But providers are not the only ones seeing their roles change. Compliance officers, who serve as organizations’ internal police officers, will have many new challenges. PYA Principal Martie Ross presented a national Health Care Compliance Association (HCCA) webinar entitled “The Evolving Role of the Compliance Officer In the Age of Accountable Care.”
The Heartaches Associated with Billing for Cardiac DevicesPYA, P.C.
PYA Principal Denise Hall-Gaulin and Consulting Manager Joanna Malcolm presented a free webinar for the Georgia chapter of the Healthcare Financial Management Association, on Tuesday, December 6, 2016.
The presentation was geared toward C-suite hospital leaders, compliance officers, in-house counsel, operational leaders, and patient accounting leadership, and covered:
The criteria for implantable cardioverter defibrillators (ICDs), pacemakers, and other devices
The documentation requirements for payment
The prerequisites for a clean audit
Accounting Update Overview with a Healthcare SlantPYA, P.C.
PYA Principal and Director of Audit Services Doug Arnold presented during East Tennessee State University’s 38th Annual Accounting, Auditing, and Tax Updating CPE conference. His presentation covered many recent Accounting Standards Updates, but leaned toward their applications in healthcare.
This document outlines the 10 steps of the medical billing cycle: 1) preregistering patients, 2) establishing financial responsibility, 3) checking patients in, 4) checking patients out and documenting diagnoses and procedures, 5) reviewing coding for compliance, 6) checking billing compliance, 7) preparing and transmitting insurance claims, 8) monitoring payer adjudication of claims, 9) generating patient statements, and 10) following up on payments and handling collections. It also defines key terms related to medical billing and coding.
Presentation Explores Many Contexts of Community BenefitPYA, P.C.
PYA Principal David McMillan gets to the bottom of the definition of community benefit in “Community Benefit: One Term, Many Contexts,” a presentation given at the 2013 AICPA Healthcare Industry Conference.
Presentation Uncovers Trends in the Unpredictable Healthcare IndustryPYA, P.C.
With the healthcare industry in a state of flux, not much is known about what lies ahead; but trends across the industry have become apparent and are likely to stick. These trends were the subject of a presentation given by PYA Principal David McMillan at the PKF North America Healthcare Fly-In.
PYA Healthcare Consulting Senior Manager Robert Mundy co-presented during, “Valuing Hospitals,” Thursday, July 31, at 1 p.m. EST. This webinar explores the changing world of hospital economics, regulations, and valuations and how appraisers can best prepare themselves for both the opportunities and challenges that lie ahead.
PYA Highlights Next Steps of Meaningful UsePYA, P.C.
At the 2013 AICPA Healthcare Industry Conference, PYA Principal David McMillan and Senior Manager Chris Wilson recently explored the “new normal” of meaningful use as compliance and strategic standards in new care/reimbursement-model development.
The document discusses the drivers and pressures for organizational change. It identifies that change comes from both external environmental pressures such as competition, regulations and technological changes as well as internal pressures like growth, leadership changes, and politics. Some of the key external pressures mentioned are globalization, hypercompetition, and reputation concerns. The document also examines why organizations may not change in response to environmental pressures or after crises, citing factors such as organizational learning difficulties and defensive priorities over innovation.
This document discusses evolutionary developmental biology and how changes in development can lead to evolutionary changes. It provides examples of modularity and molecular parsimony which help explain this. Modularity means parts of the body and DNA can develop differently. Molecular parsimony means organisms share developmental toolkit genes. The document then discusses specific examples like stickleback fish pelvic spines being due to different Pitx1 expression, and Darwin's finches having beak shape variations due to differing Bmp4 and Calmodulin expression levels. Mechanisms of evolutionary change include changes in location, timing, amount, or kind of gene expression.
Developmental plasticity allows an organism's phenotype to change in response to environmental conditions during development. There are two main types of phenotypic plasticity: reaction norms, where the environment determines the phenotype from a continuum of genetic possibilities, and polyphenisms, where discrete alternative phenotypes are produced. Examples include caterpillars changing appearance to match plant growth stages, frogs hatching early in response to vibrations, and temperature determining sex in crocodiles. Stressors like water levels can also influence development, as seen in spadefoot toads. Symbiotic relationships between organisms, like nitrogen-fixing bacteria in plant roots, are important to development and often involve vertical transmission from parents. Gut bacteria are also necessary for
This document discusses several genetic and environmental factors that can influence human development. Genetic factors like pleiotropy and mosaicism can result in syndromes with multiple abnormalities. The same genetic mutation can also produce different phenotypes depending on gene interactions. Environmental teratogens during critical periods of embryonic development can irreversibly damage organ formation, with alcohol, retinoic acid, and endocrine disruptors like bisphenol A and atrazine posing particular risks like fetal alcohol syndrome, cleft palate, lower sperm counts, and cancer. Both genetic and environmental heterogeneity contribute to the complexity of human development.
The endoderm forms the epithelial lining of the digestive and respiratory systems. It gives rise to tissues like the notochord, heart, blood vessels, and parts of the mesoderm. The endoderm comes from two sources - the definitive endoderm and the visceral endoderm. The transcription factor Sox17 marks and regulates the formation of the endoderm. The endoderm lines tubes in the body and gives rise to organs like the liver, pancreas, lungs and digestive system through the formation of buds and pouches along the foregut.
The document summarizes the development of the intermediate mesoderm and lateral plate mesoderm. The intermediate mesoderm forms the urogenital system including the kidneys, ureters, ovaries, fallopian tubes, testes and vas deferens. Kidney development occurs through the pronephros, mesonephros and metanephros stages. The lateral plate mesoderm splits into somatic and splanchnic layers and forms the heart through the merging of cardiac progenitor cells from both sides of the embryo. The heart tube loops to the right to begin resembling the four-chambered adult heart.
The paraxial mesoderm lies just lateral to the notochord and gives rise to vertebrae, skeletal muscles, and skin connective tissue. It is divided into somites which then form dermomyotomes and sclerotomes. Dermomyotomes develop into dermatomes that make dermis and myotomes that form back, rib, and body wall muscles. Sclerotomes form the vertebrae and rib cage. Somitogenesis occurs through a clock-wavefront model where somites sequentially segment from cranial to caudal regions under the influence of signaling molecules like retinoic acid and FGF.
The document summarizes ectodermal placodes and the epidermis. It discusses how placodes give rise to sensory structures like the eye lens, inner ear, and nose. It describes the different cranial placodes that form sensory tissues and nerves, including the anterior placodes that form the pituitary gland and eye lens. The intermediate placodes form nerves involved in sensation of the face and hearing/balance. The epidermis derives from surface ectoderm under the influence of BMPs and forms the protective outer layer of skin and its appendages like hair, sweat glands, and teeth.
- The neural plate transforms into a neural tube through a process called neurulation regulated by proteins like BMP and transcription factors like Sox1, 2, and 3.
- Primary neurulation involves the elongation, bending, and convergence of the neural folds before their closure at the midline to form the neural tube. Key regulation events involve hinge points at the midline and dorsolateral edges.
- Neural tube defects can occur if closure fails, as in spina bifida where the posterior neuropore remains open, preventing proper spinal cord development.
Mammalian development begins with fertilization and cleavage of the egg. The egg develops membranes that allow development outside of water. In mammals, the placenta exchanges gases and nutrients between the embryo and mother. Cleavage is rotational, with zygotic genes activating later than other animals. Cells compact and the morula forms an inner cell mass and trophoblast cells. The trophoblast secretes fluid to form a blastocyst cavity. The inner cell mass forms the epiblast and hypoblast, which generate the embryo and extraembryonic tissues through gastrulation. Axis formation is guided by gradients of genes like HOX and left/right asymmetries are regulated by proteins including Nodal.
- Drosophila melanogaster is a useful model organism for studying development due to its short life cycle, fully sequenced genome, and ease of breeding.
- Early Drosophila development involves syncytial cleavage where nuclei divide without cell division, specifying the dorsal/ventral and anterior/posterior axes.
- Fertilization occurs when sperm enters an egg that has already begun specifying axes; maternal and paternal chromosomes remain separate during early divisions.
This document summarizes key patterns in animal development. It describes that animals undergo gastrulation where cells migrate to form germ layers and axes. Animals are categorized into 35 phyla based on features like germ layers, organ formation, and cleavage patterns. It describes that diploblastic animals have two germ layers while most are triploblastic with three germ layers. Triploblastic animals are further divided into protostomes and deuterostomes based on mouth formation. The document also provides examples of cleavage patterns in snails which are spirally arranged in either a dextral or sinistral pattern determined by maternal factors.
1) Sex determination in mammals is primarily determined by the XY sex determination system, with females having XX and males having XY. The SRY gene on the Y chromosome causes the development of testes.
2) The gonads are initially bipotential but develop into either ovaries or testes based on the sex chromosomes. Testes secrete AMH and testosterone to direct male development while ovaries secrete estrogens for female development.
3) Gametogenesis includes the process of meiosis which produces haploid gametes from diploid germ cells in the gonads. In females, oogenesis begins in the embryo but arrests until puberty while spermatogenesis only occurs at puberty in males.
Stem cells are unspecialized cells that can divide and differentiate into specialized cell types. There are several types of stem cells defined by their potency, including totipotent stem cells found in early embryos, pluripotent stem cells in the embryo, and multipotent adult stem cells. Stem cell regulation is controlled through extracellular signals from the stem cell niche and intracellular factors that influence gene expression and cell fate. Researchers have also induced pluripotency in adult cells by introducing genes that code for key transcription factors.
This document discusses cell-to-cell communication and how it allows for the development of specialized tissues and organs through three main mechanisms: cell adhering, cell shape changing, and cell signaling. It describes how cells interact at the cell membrane through various receptor and ligand proteins. These interactions can be homophilic or heterophilic, and occur through direct contact between neighboring cells (juxtacrine signaling) or over short distances (paracrine signaling). Differential adhesion and cadherins allow cells to sort themselves into tissues based on adhesion strengths. The extracellular matrix and integrins also influence cell communication and development.
Differential gene expression refers to the process where different genes are activated in different cell types, leading to cellular specialization. While all cells contain the full genome, only a small percentage of genes are expressed in each cell. Gene expression is regulated at multiple levels, including differential transcription, selective pre-mRNA processing, selective mRNA translation, and posttranslational protein modification. The most common mechanisms involve regulating transcription through epigenetic modifications of chromatin and the use of transcription factors.
The document summarizes key stages in animal development from fertilization through organogenesis. It begins with fertilization and cleavage, followed by gastrulation where the three germ layers (endoderm, mesoderm, ectoderm) are formed. During organogenesis, organs develop from the germ layers. Metamorphosis may also occur to transition organisms like frogs from immature to sexually mature forms. Examples are provided of developmental processes in frogs and other model organisms like fruit flies and plants. Cell behavior and patterning during these stages are also discussed.
The document discusses considerations for small businesses when hiring employees. It covers deciding when to hire an employee, defining job roles, writing job descriptions, attracting and evaluating candidates, selecting the right hire, training employees, rewarding and compensating employees, and managing ownership and dividends when there are family business partners involved. The key aspects of setting up an employee program for a small business are planning job roles, writing thorough job descriptions, developing fair hiring and review processes, providing training, and establishing clear compensation and ownership structures.
This document discusses various legal issues that small business owners should be aware of, including:
- Understanding the different types of laws (federal, state, local) that may apply to a small business.
- Hiring an experienced small business attorney to provide legal advice and represent the business as needed.
- Choosing an appropriate legal structure for the business, such as a sole proprietorship, partnership, corporation, or LLC.
- Protecting the business name as intellectual property and complying with regulations regarding contracts, liability, taxation and other legal matters.
This document discusses risk management and insurance for small businesses. It begins by defining risk for business owners and identifying common sources of risk such as financial investments, theft, nonpayment of debts, and natural disasters. It then examines risks related to a business's property, personnel, customers, and intangible property. The document provides strategies for managing these risks, such as developing policies and procedures, securing valuable assets, and obtaining different types of insurance. It concludes by discussing ways for businesses to share risk through joint ventures, industry groups, and government funding programs.