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HEPATOMA DISEASE IN RAINBOW
TROUT
Presented By
Pabitra Pandey
BSc. Fisheries
11/7/2023 1
List of contents
• Introduction
• Symptoms
• Control measures
• References
11/7/2023 2
Introduction
• Hepatoma disease is also known as liver cancer
• Etiological agent: deposition of aflatoxin in liver(derived from
Aspergillus flavus)
• Trout are poikilothermous and prefer waters with temperatures from
6 to 20 C
• Hepatomas develop more rapidly in fish reared at the higher
temperatures
11/7/2023 3
Cont…
• Fish which are regularly feeding on commercial feeds containing oil
cause hepatoma
• Rainbow trout hepatoma was found to be widespread in hatcheries
and fish farms in 1960
• In 1961, one common denominator noted as a possible vector in
trout hepatoma was dietary oil seed meals
• Cottonseed meal from commercially pelleted fish feeds was found to
contain a carcinogenic factor
11/7/2023 4
Cont…
• Crystalline aflatoxins fed to trout at 0.5 to 20 ppb in a purified test
diet induced a high incidence of trout hepatoma (Halver, 1965)
• If aflatoxin levels are high (1 to 15 mg/kg body weight of aflatoxin
B1),
• Fish may die in 4 to 18 days with acute hepatitis (aflatoxicosis) before
tumors can develop
• Livers of such fish show varying degrees of hepatocyte degeneration,
necrosis and hemorrhage
11/7/2023 5
Symptoms
• Clinical signs are not usually apparent until the disease is advanced
• Fish may be listless and irritable, but diseased fish continue to feed
• Liver discoloration and the appearance of numerous small nodules in
the liver
• Infected liver enlarge from its normal size
11/7/2023 6
• Disease fish, specially female, may show distress and often die
during soon after spawning. Mortality among disease brood
stock may reach 100%
11/7/2023 7
Control measures
• Fish feed must be free from aflatoxin
• Need to keep it in suitable dry and ventilated storage
• Destroy the contaminated feed stock
11/7/2023 8
Pictures
11/7/2023 9
References
• Fisheries, B. O. S. (1969). The Western Fish Nutrition
Laboratory (No. 81). US Fish and Wildlife Service.
• Halver JE: Aflatoxicosis and rainbow trout hepatoma,
Symposium on Mycotoxins and Foodstuffs, 1964 Proceedings.
Cambridge, Mass, Massachusetts Institute of Technology
Press, 1965, pp 209-234
11/7/2023 10
11/7/2023 11

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Hepatoma Disease in Rainbow Trout.pptx

  • 1. HEPATOMA DISEASE IN RAINBOW TROUT Presented By Pabitra Pandey BSc. Fisheries 11/7/2023 1
  • 2. List of contents • Introduction • Symptoms • Control measures • References 11/7/2023 2
  • 3. Introduction • Hepatoma disease is also known as liver cancer • Etiological agent: deposition of aflatoxin in liver(derived from Aspergillus flavus) • Trout are poikilothermous and prefer waters with temperatures from 6 to 20 C • Hepatomas develop more rapidly in fish reared at the higher temperatures 11/7/2023 3
  • 4. Cont… • Fish which are regularly feeding on commercial feeds containing oil cause hepatoma • Rainbow trout hepatoma was found to be widespread in hatcheries and fish farms in 1960 • In 1961, one common denominator noted as a possible vector in trout hepatoma was dietary oil seed meals • Cottonseed meal from commercially pelleted fish feeds was found to contain a carcinogenic factor 11/7/2023 4
  • 5. Cont… • Crystalline aflatoxins fed to trout at 0.5 to 20 ppb in a purified test diet induced a high incidence of trout hepatoma (Halver, 1965) • If aflatoxin levels are high (1 to 15 mg/kg body weight of aflatoxin B1), • Fish may die in 4 to 18 days with acute hepatitis (aflatoxicosis) before tumors can develop • Livers of such fish show varying degrees of hepatocyte degeneration, necrosis and hemorrhage 11/7/2023 5
  • 6. Symptoms • Clinical signs are not usually apparent until the disease is advanced • Fish may be listless and irritable, but diseased fish continue to feed • Liver discoloration and the appearance of numerous small nodules in the liver • Infected liver enlarge from its normal size 11/7/2023 6
  • 7. • Disease fish, specially female, may show distress and often die during soon after spawning. Mortality among disease brood stock may reach 100% 11/7/2023 7
  • 8. Control measures • Fish feed must be free from aflatoxin • Need to keep it in suitable dry and ventilated storage • Destroy the contaminated feed stock 11/7/2023 8
  • 10. References • Fisheries, B. O. S. (1969). The Western Fish Nutrition Laboratory (No. 81). US Fish and Wildlife Service. • Halver JE: Aflatoxicosis and rainbow trout hepatoma, Symposium on Mycotoxins and Foodstuffs, 1964 Proceedings. Cambridge, Mass, Massachusetts Institute of Technology Press, 1965, pp 209-234 11/7/2023 10