George Harper is a chemical engineering student at North Carolina State University seeking a position in biomanufacturing. He has a 3.25 GPA and will graduate in May 2016 with a B.S. in Chemical Engineering and a minor in Biotechnology and Tissue Engineering. His experience includes undergraduate research utilizing molecular biotechnology techniques like DNA manipulation and protein purification. He has presented his research at several conferences and is published as a coauthor. Harper also has experience as a teaching assistant and engineering consultant.

1. George Harper
gwharpe2@ncsu.edu
704-975-2801 (Cell)
Objective:
To obtain a position in a biomanfacturing company where I can apply my technical research and interpersonal skills to
various clinical and engineering challenges.
Education:
North Carolina State University: B.S. in Chemical Engineering - Biomolecular Concentration (Expected
May 2016)
o Overall GPA: 3.25/4.0
o Minor in Biotechnology and Tissue Engineering
o Member of the NCSU Scholars Program: (Fall 2012 - Present)
o Dean’s List: Spring 2013 and Spring 2014
Experience:
 Undergraduate Research at NCSU in Dr. Robert Kelly’s Hyperthermophile Lab Group: (Summer 2015 - Present)
o Utilized various molecular biotechnology techniques - plasmid and genomic DNA purification
o DNA transformation and E. coli manipulation
o Purified proteins using affinity chromatography FPLC techniques
o Conducted in vivo genetic manipulations in the hyperthermophilic bacterium Caldicellulosiruptor bescii
o Examined the activity of biomass degradation enzymes in vitro using various assays and HPLC techniques
o Prepared samples for immunofluorescence microscopy using primary and secondary antibody labeling
o Performed recombinant protein expression in E. coli shake flask cultures
o Participated at weekly group lab meetings including periodic presentations
 Volunteer Teacher’s Assistant - Organic Chemistry 1& 2 with Dr. Kay Sandberg: (Fall 2014 & Spring 2015)
o Facilitated learning in a dynamic and interpersonal teaching environment
o Organized and led independent review sessions before each exam
 Burke Design Group Engineering Consultant: (Summer 2014)
o Produced industrial floor plans using DataCAD as a part of a collaborative team for time sensitive client projects
Publications and Presentations:
 Coauthor of:
o Modular Surface-layer Homology (SLH) Domain Glycoside Hydrolases (Calkro_0111 and Calkro_0402)
Contribute to Plant Cell Wall Deconstruction by the Extremely Thermophilic Bacterium Caldicellulosiruptor
kronotskyensis
 Research Poster presentation:
o AIChE’s National Conference in Salt Lake City, Utah (November 2015)
o State of North Carolina Undergraduate Research and Creativity Symposium (November 2015)
o North Carolina State University Undergraduate Summer Research Symposium (July 2015)
Laboratory Courses:
 BME 483: Tissue Engineering Technologies
o Constructed, maintained, and assayed angiogenic response in bi-layered collagen based living skin equivalent by layering
normal human epidermal keratinocytes on top of a human dermal fibroblast 3-D cell culture.
 BIT 566: Animal Cell Culture
o Demonstrated proper aseptic technique while isolating, maintaining, counting, and freezing animal cells in culture.
o Established primary myoblast cell culture from chick embryos.
 BIT 471: RNA Interference
o Produced phenotypes via RNAi genetic knock-downs in C.ellegans, N.bethamiana tobacco plants, and HeLa cells.
 BIT 562: Microarray Analysis
o Experience with JmpGenomics analyzing and trending microarray data
 BIT 410: Manipulation of Recombinant DNA
Activities & Skills:
 Member of the American Institute of Chemical Engineers (AIChE) student chapter: (Fall 2012 - Spring 2013 & Fall 2015
– Present)
 Microsoft Excel Spreadsheets applying non-linear equation solving, Solver, and VBA programing
Permanent Address
8705 Man of War Drive
Waxhaw, NC, 28173
Current Address
Unit 1420, 2730 Wolf Village Way
Raleigh NC, 27607