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Dr. Tahir Abbas Baloch
Ph.D. Zoology (Punjab University, Lahore)
tahirabbasbloch@gmail.com 03339810179
Dr. Tahir Abbas Baloch You Tube
https://www.youtube.com/channel/UC-
9mb8kyILhPKKl9PwAdvbw?view_as=subscriber
Previous Lecture Genetics: 10, 11,12
Genetics, Mendelian Inheritance
Dr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch You Tube
https://www.youtube.com/channel/UC-
9mb8kyILhPKKl9PwAdvbw?view_as=subscriber
Dr. Tahir Abbas Baloch
MDCAT, NUMS, PPSC
13
A A
B b
HOMOZYGOUS
HETEROZYGOUS
HETEROZYGOUS (AABb)
A A
bB
A A
bB
A A
bB
Un – replicated
Replicated
ALLELES
NOT- ALLELES
NOT- ALLELES
Bacteria, have a single ring of DNA, have one allele per gene per organism
Chiasma/Synaptonemal Complex
Synapse/Synapsis
Centromere
Primary
Constriction
Sec Constriction
Junk Gene –non coding/Heterochromatin/Euchromatin
TELOMERS TELOMERS
Dr. Tahir Abbas Baloch
coupling/cisphase
trans phase
REPULSION PHASE
Crossing Over
Recombinants
*Coupling
Reaction
Sister Chromatids
Daughter Strands
Replicated Forms
transposonorjumpinggene
GENES A, B, D, E, F, H, I, R, T, W, X, Y, Z, Rh, SRY, W+…… Dominant Genes
a, b, d, e, f, h, i, r, t, w, X, Y, Z, Rh, SRY, ……….…… Recessive Genes
AA, BB, DD, EE, FF, HH, II, RR, TT, W+ W+, XX, YY, ZZ, …… Allele
Aa, Bb, Dd, Ee, Ff, Hh, Ii, Rr, Tt, W+ w, Xx, Yy, Zz, …………. Allele
aa, bb, dd, ee, ff, hh, ii, rr, tt, w w, xx, yy, zz, ………………... AlleleALLELES SINGLE ALLES
AA1A2a, BB1b, CC1C2C3, D1D2D3D4 --------------
AaBbCc ---------------------------------------------------- POLYGENES/ Polygeny/Polygenic
AA, Aa, aa,
A1A1, AA2,
A1A2, AA1
A1a, A2a
Gene Pool of Populations
A, A1, A2, a
4
Individual = 9
Genes= 2n= 18
AA, Aa, aa,
A2A1, AA1,
A1A1, AA1
A1a, aA1
Gene Flow
A2
AA
A2A1
Bottle Neck Effects
Genetic Drift
AA, AA2, A2A2, A1A1,
AA2, A1A2, AA1, A1A,
A2A, AA, AA2, A2A2,
A1A1, AA2, A1A2, AA1,
A1A, A2A, A1A2, AA1
A, A1, A2
3
Population Density
PopulationPressure
A
Gene
a
A1
A2
Alles
Mutation
Genetic Diversity
Gene Pool DeclineDr. Tahir Abbas Baloch
DOUBLE ALLES
MULTIPLE ALLES
GENE:
 unit of heredity transferred from parent to offspring.
 sequence of nucleotides in DNA /RNA determines order of monomers in polypeptide
 basic physical and functional unit of heredity.
 unit of hereditary information that occupies a fixed position on a chromosome.
 bacteria gene is more complex than eukaryotes.
 plasmids, contain (usually) non-essential genes.
 viral genes are mostly present in a single molecule of nucleic acid.
 Females have more genes & more complex genome than male due to XX
 LOCUS is a position of genes on a chromosome.
GENE MARKER
(Probe)
DNA MARKER
Molecular Marker
Morphological
Marker
 Transposable element (transposon, or jumping gene) DNA sequence that can
change its position within a genome.
 Gene silencing is regulation of gene in a cell to prevent their expression.
LOCUST Dr. Tahir Abbas Baloch
 Wilhelm Johannsen coined the word “ GENE "
Wilhelm Johannsen describe the Mendelian units of heredity (gene), expressed as phenotype and
genotype.
Mendel is Considered Father of Genetics
SelfingDusting
Dr. Tahir Abbas Baloch
Mendel First Law
Mendel Law of Inheritance
Law of Dominance
Monohybrid Law
Law of Purity of Gametes
Phenotypic Ratio 3: 1
Genotypic Ratio 1: 2: 1
Genotypic Ratio of Homozygous Dominant 1/4
Genotypic Ratio of Heterozygous 2/4 = ½= 0.5
Genotypic Ratio of Homozygous Recessive 1/4
Dr. Tahir Abbas Baloch
P 1
F 1
F 2
F 3Dr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch
Mendel First Law
Round = ¾ Yellow = ¾
Wrinkle = ¼ Green = ¼
Product Rule
Round x Yellow = ¾ x ¾ = 9/16
Round x Green = ¾ x ¼ = 3/16
Wrinkle x Yellow = ¾ x ¼ = 3/16
Wrinkle x Green = ¼ x ¼ = 1/16Tall = ¾ Short = ¼
Round x Tall = ¾ x ¾ = 9/16
Round x Short = ¾ x ¼ = 3/16
Wrinkle x Tall = ¾ x ¼ = 3/16
Wrinkle x Short = ¼ x ¼ = 1/16
The probability of two or
more independent events
are equal to the product of
their individual probabilities.
1 : 2 : 2 : 4 : 1 : 2 : 1 : 2 : 1 genotypes = 9 Total = 169 : 3 : 3 : 1 phenotypes = 4
F 2
F 1
P 1
LAW OF INDEPENDENT ASSORTMENT
Male gametes 4
Femalegametes4
4 x 4 = 16
Meiosis
Gametogenesis ½ 0.5
DihybridSelf Cross F1 x F1
AaBb
Dihybrid
aabb
Recessive
AB, Ab, aB, ab
Gametes
ab
Gametes
AaBb, Aabb, aaBb,aabb
1 : 1 : 1 : 1
P 1
F 1
Diallelic Characters
Dihybrid Characters
Test Cross
Complete Dominance
Law of Independent
Assortment
Single Crossing Over
Recombination
Dr. Tahir Abbas Baloch
AABBCC
Dark Red
aabbcc
White
ABC
Gametes
abc
Gametes
AaBbCc
P 1
F 1
Triallelic Characters
Trihybrid Characters
Test Cross
Polygeny
Continuously varying
Traits
Recombination
Dr. Tahir Abbas Baloch
Trihybrid
Light Red Incomplete Dominance
Wheat grain color
AaBbCc
Light Red
AaBbCc
F 1 x F1
Meiosis
Gametes
ABC ABc AbC Abc
2
n
aBC aBc abC abc
ABC
ABC
ABc
ABc
AbC
AbC
Abc
Abc
aBC
aBC
aBc
aBc
abC
abC
abc
abc
ABC
ABC
ABC
ABc
ABC
AbC
ABC
Abc
ABC
aBC
ABC
aBc
ABC
abC
ABC
abc
ABc
ABC
ABc
ABc
ABc
AbC
ABc
Abc
ABc
aBC
ABc
aBc
ABc
abC
ABc
abc
AbC
ABC
AbC
ABc
AbC
AbC
AbC
aBC
AbC
aBc
AbC
abC
AbC
abc
AbC
Abc
Abc
ABC
Abc
ABc
Abc
AbC
Abc
aBC
Abc
aBc
Abc
abC
Abc
abc
Abc
Abc
aBC
ABC
aBC
ABc
aBC
AbC
aBC
aBC
aBC
aBc
aBC
abC
aBC
abc
aBC
Abc
aBc
ABC
aBc
ABc
aBc
AbC
aBc
aBC
aBc
aBc
aBc
abC
aBc
abc
aBc
Abc
abC
ABC
abC
ABc
abC
AbC
abC
aBC
abC
aBc
abC
abC
abC
abc
abC
Abc
abc
ABC
abc
ABc
abc
AbC
abc
aBC
abc
aBc
abc
abC
abc
abc
abc
Abc
6
012 11
1
1
1
22
2
2
2
2
2
22
2
5 5 4 5 4 4 3
5 4 4 3 4 3 3
5 4 4 3 4 3 3
2232334
5 4 4 3 4 3 3
4 3 3 2 3
3334
3
Dose Genotypes Phenotypes Ratio
0 aabbcc White 1/64
1 Aabbcc (2) aaBbcc (2) aaBbcc (2) 6/64
AABBC AABCC AABC ABBCC ABBC ABCC ABC
AABBC AABB AABC AAB ABBC ABB ABC BC
AABCC AABC AACC ABCC ABC ACC ACACC
AABC ABC AAC ABC AB ACAA
ABBCC ABBC ABBC BBCC BBC BCC BCABC
ABBC ABB ABC BBC BB BCAB
ABCC ABC ACC BCC BC CCAC
ABC AB AC BC
2 AA(1)
A
B
C
0BB
AB (3) AC (4)BC(5)BB(1) CC(1) 15/64
3 BCC (2)ACC (2)
ABC (9)
ABB (2) BBC (2) AAC (2)AAB (1) 20/64
4 ABCC (3)
ABBC (5)
BBCC (1) AABC (4)AACC (1) AABB (1) 15/64
5 ABBCC (2) AABCC (2) AABBC (2) 6/64
6 AABBCC 1/64
1
3
6
7
6
3
1
Light Pink
Pink
Light Red
Red
Moderate Dark Red
Dark Red
White = 1/64
Light Pink = 3/32
Pink = 15/64
Light Red = 5/16
Red = 15/64
Moderate = 3/32
Dark Red = 1/64
7
Designed by;
Dr. Tahir Abbas
F2
Ratio, Proportion, Probability
Monohybrid First Law Phenotype in F 2 is 3:1, Genotypic 1:2:1
Dihybrid/Second Law Phenotype 9:3:3 :1, Genotype 1:2:2:4:1:2:1:2:1
Incomplete Dominance Phenotype & Genotype 1:2:1
Trihybrid/Polygeny Phenotype 1:6:15:20:15:6:1
Monohybrid First Law, Phenotype in F 1 is 1:1:1:1
Dihybrid Second Law, Phenotype in F 1 is 1:1:1:1
Trihybrid Second Law, Phenotype in F 1 is 1:1:1:1
Test Cross for Homozygous is always 100%
Test Cross for Heterozygous is # 100% but not more than 50 %
T t Aa Bb Rr Yy Complete Dominance
Dominant 100 % in F1, Recessive in F 1 0 %
R1 R2 R1R2 In Complete Dominance
Dominant 50 % in F1, Recessive in F 1 50 %
A B AB
Dominant 100 % in F1, Recessive in F 1 100 %
Co-Dominance
A a
Traits - I
Traits - II
Pleiotropy
Chromosome 9 Chromosome 19
EPISTASIS Non-Homologous Dominance
Polygeny/Contineously Varying Traits
AaBbCc /Trihybrids
F 1
F 2
1:6:15:20:15:6:1
Phenotypes = 7
Multiple Allele
Blood Group
Gene ABO
Dr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch
Sutton and Boveri, states chromosomes are the vehicles of genetic heredity; beave as
segregation, independent assortment, and linkage.
Chromosomal Theory
Gene theory - genes are the basic units in which characteristics are passed from one
generation to the next.
Neutral Gene theory, the behavior of mutant genes in populations is determined by
random genetic drift/bottle neck effect.
Thomas Hunt Morgan won the Nobel Prize in 1933 prove that rule by Drosophila.
LINK
Gene to Gene Linkage Gene to Chromosome Link
Genetic Linkage
Linkage Group
Homologous Pairs
Genes of Chromatid
Autosomal Linkage Sex Linked Genes
Male = Female Male # Female
X-Linked Genes Y-Linked Genes
Only in MaleX-Linked Dominant Genes
X-Linked Recessive GenesMale ≥ Female
Female ≥ Male
Pseudoautosomal Gene
on mammalian X and Y
chromosomes e.g.
Mammary Gland Genes
Hemophilia
Red-green
Blindness
White Eye
Color in
Drosophila
Baldness
Hypophosphatemic ricket
Blood Group Gene
X X
W+ W+
Female Red Eye
X X
W+ w
Hetrozygous, Carrier Female Red Eye
X Y
W+
Male Red Eye
X X
w w
Female White Eye
X Y
w
Male white Eye
X X
X , X
Eggs
X Y
X , Y
Sperms
HOMOGAMETIC HETROGAMETIC
WILD TYPE (Red Eye)
AUTOSOME
A = 2n-2
Sex Chromosome
ALLOSOMES
X X
W+ W+
Female Red Eye
X X
W+ w
Female Red Eye
X X
w w
Female White Eye
X Y
W+
Male Red Eye
X Y
w
Male White Eye
Female/Male Red Eye Female/Male Red Eye
2 Female/1 Male Red Eye
2 Female Red Eye
2 Male White Eye
1 Male White Eye
Female/Male Red Eye
Female / Male White Eye
Female / Male White Eye
Parental Cross Test Cross
Test Cross
Back Cross
Reciprocal Cross Confirmatory Cross
Self Cross = F 1 x F 1
= F 2 x F 2
X X
W+ W+
Female Red Eye
X X
W+ w
Hetrozygous, Carrier Female Red Eye
X Y
W+
Male Red Eye
X X
w w
Female White Eye
X Y
w
Male white Eye
Dr. Tahir Abbas Baloch
X X
W+ w
Hetrozygous, Carrier Female Red Eye
X Y
w
Male white Eye
X X
W+ W+
Female Red Eye
X Y
w
Male white Eye
Parental Cross P 1
X , X
W+ W+
X , Y
w
X X
W+ w
Hetrozygous, Carrier Female Red Eye
X Y
W+
Male Red Eye
SelfCrossF1xF1
X , X
W+ w
X , Y
W+
X X
W+ W+
Female Red Eye
X Y
W+
Male Red Eye
X X
W+ w
Hetrozygous, Carrier Female Red Eye F 2
X X
W+ w
Hetrozygous, Carrier Female Red Eye
X Y
w
Male White Eye
TEST CROSS
X , X
W+ w
X , Y
w
Gametes by Meiosis
X X
W+ w
Hetrozygous, Carrier Female Red Eye
X Y
W+
Male Red Eye
X X
w w
Female White Eye
X Y
w
Male White Eye
X Y
w
Male White Eye
RECIPROCAL
CROSSX X
w w
Female
White Eye
X Y
W+
Male Red Eye
X , X
w w
X , Y
W+
P1 = F(R) x M (W) → 2 F (R,C), 2 M (R)
Parental Cross 2:2, 1: 1, 100 %
F1xF1 = F(R,C) x M (R) → 1 F (R,C), 1 M (R)
Self Cross 1 F (R) , 1 M (W)
3:1 (R,W)/75%, 25%, 100% (F), 50% (M)
Test Cross = F(R,C) x M (W) → 1 F (R,C), 1 M (R)
1 F (W) , 1 M (W)
2:2 (R,W) or 1: 1 50% (F), 50% (M)
Reciprocal Cross = F(W) x M (R) → 2 F (R,C), 2 M (W)
1:1 (R,W) or 50%, 100% (F), 100% (M white)
One gene one enzyme
One gene polypeptide
Sex Determination
Chromosomal Theory
Gene Theory
Sutton Theory
T.H Morgan Work
X-Linked Recessive Trait Page Prepared By Dr. Tahir Abbas Baloch
F 1
X X
W+ W+
Female Red Eye
X Y
w
Male White Eye
Gametes
X , X
W+ W+
X , Y
w
X X
W+ w
Female Red Eye
Carrier
X Y
W+
Male Red Eye
2 F (R) : 2 M (R)
1 : 1
overall 100% Red eyes
ParentalCross
Reciprocal Cross
Phenotypically inverse to P1
Male Red Eye
overall 50% Red eyes
Female 100% Red
Male 100 % White Eye
Test Cross
Test Cross
X X
H h
Heterozygous, Carrier Female
X Y
h
Hemophiliac Male
X X
H H
Normal Female
X Y
h
Hemophiliac Male
Parental Cross P 1
X , X
H H
X , Y
h
X X
H h
Heterozygous, Carrier Female
X Y
H
Normal Male
X , X
H h
X , Y
H
X X
H H
Normal Female
X Y
H
Normal Male
X X
H h
Heterozygous, Carrier Female F 2
X X
H h
Heterozygous, Carrier Female
X Y
h
Male Hemophiliac
TEST CROSS
X , X
H h
X , Y
h
Gametes by Meiosis
X X
H h
Heterozygous, Carrier Female
X Y
H
Normal Male
X X
h h
F Hemophiliac
X Y
h
M Hemophilic
X Y
h
Male Hemophiliac
RECIPROCAL
CROSSX X
h h
Female
Hemophiliac
X Y
H
Normal Male
X , X
h h
X , Y
H
P1 = F(N) x M (H) → 2 F (N,C), 2 M (H)
Parental Cross 2:2, 1: 1, 100 %
F1xF1 = F(N,C) x M (N) → 1 F (N,C), 1 M (N)
Self Cross 1 F (N) , 1 M (H)
3:1 (N,H)/75%, 25%, 100% (F), 50% (M)
Test Cross = F(N,C) x M (H) → 1 F (N,C), 1 M (N)
1 F (H) , 1 M (H)
2:2 (N,H) or 1: 1 50% (F), 50% (M)
Reciprocal Cross = F(H) x M (N) → 2 F (N,C), 2 M (H)
1:1 (N,H) or 50%, 100% (F), 100% (M H)
One gene one enzyme
One gene polypeptide
Sex Determination
Chromosomal Theory
Gene Theory
Sutton Theory
T.H Morgan Work
X-Linked Recessive Trait Page Prepared By Dr. Tahir Abbas Baloch
F 1
Gene recombination is naturally occurring during meiosis in eukaryotes, involves the pairing of homologous
chromosomes and information transfer between the chromosomes.
A
B
a
b
AB ab Ab aB
AB ab
Non-Parental α Crossing Over α Recombination α Genetic Map α Gene Distance
A B
20
A B
20
C
15
35
A B
15
A B
15
C
?
55 ? = 40
A
B
a
b
A
B
a
b
A
B
Synapsis Chiasma
A
B B
a
AB aB
a
bb
A
Ab ab
NP (recombinant)
P P
a
b
a
b
ab
P
AB aB Ab ab
abAa
Bb
aa
Bb
Aa
Bb
aa
bb
PP NP NP
Recombinant Frequency = Σ Non-Parental x 100 = Genetic Map
Total
4x1=4
P = 40+40=80 NP = 10+10=20
A
B
AB
O
IA IA
IA i
IB IB
IB i
IA IB
ii
+
DD
Dd _
dd
+
DD
Dd _
dd
+
DD
Dd _
dd
+
DD
Dd _
dd
PHENOTYPES
GENOTYPE (ABO)GENOTYPE (ABO-Rh)
12
6
4
Dr. Tahir Abbas Baloch
DD Dd dd
DDDddd
+
+, -
-
+ + _
+
+
_
FATHER
MOTHER
OFFSPRINGS
DD +
Dd +
dd _
+ -h h
Dr. Tahir Abbas Baloch
+ +-
+
+, -
-+ +-
+ + +
I I
A A
I i
A
I I
B B
I i
B
I I
A B
i i
H H
H h
h h
A
A
O
B
B
O
AB
AB
O
O
O
O
9
19
EPISTASIS
BOMBAY
PHENOTYPE
ABO Group
I I
A A
I i
A
I I
B B
I i
B
I I
A B
i i9
I I
A A DD Dd dd DD Dd dd DD Dd dd DD Dd dd DD Dd dd DD Dd dd
I i
A
I I
B B
I i
B
I I
A B
i i
1
DD,Dd,dd
DD,Dd,dd
DD,Dd,dd
DD,Dd,dd
DD,Dd,ddDD,Dd,dd
Dr. Tahir Abbas Baloch
i
O A B AB
O
A
B
AB
O +
A -
A+
A+
AB+B - B+ AB -
AB+
AB-
B -
B+
A -O -
O +
O -
ii, dd, hh ii, DD, Dd, HH, Hh IAIA, IAi, dd, Hh, hh IAIA, IAi, DD, Dd, Hh, hh IBIB, IBi, dd, Hh, hh IBIB, IBi, DD, Dd, Hh, hh IAIB, dd, Hh, hh IAIB, DD, Dd, Hh, hh
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
YesYesYes
Yes
Yes
Yes
A+ A-
O+ O-
O-
O+
O-
A- O-
A+
A-
O+
O-
B- O-
B+
B-
O+
O-
A-
B-
A+ A-
B-
O+ O-
O+
A- O-
A+ O+
O-
A+
A-
O+
O-
B+
B+ O+
B- O-
B+ O+
B- O-
A+ B+
A- B-
A+ B+
A- B-
A- O-
A+ O+
A- O-
A- O-
A+ O+
A- O-
A- B-
AB- O-
A+ B+ AB+ O+
A- B- AB- O-
A- B-
AB-
A+ B+ AB+
A- B- AB-
A+ O+
A- O-
A+ O+
A- O-
A+ O+
A- O-
A+ B+AB+O+
A- B-AB- O-
A+ B+AB+ O+
A- B- AB- O-
A+ B+ AB+
A- B- AB-
A+ B+ AB+
A- B- AB-
B- O-
B+ O+
B- O-
A- B-
AB- O-
A+B+AB+O+
A-B-AB-O-
B- O-
B+ O+
B- O-
A- B- AB-
A+ B+ AB+
A- B- AB-
B+ O+
B- O-
B+ O+
B- O-
A+B+AB+ O+
A- B- AB- O-
A+B+AB+ O+
A- B- AB- O-
B+ O+
A- O-
B+ O+
A- O-
A+ B+ AB+
A- B- AB-
A+ B+ AB+
A- B- AB-
A-
O-
A+ B+
A- B-
A- B-
AB-
A+ B+ AB+
A- B- AB-
A- B- AB-
A+ B+ AB+
A- B- AB-
A- B- AB-
A+ B+ AB+
A- B- AB-
A+ A-
B+ B-
A+ A-
B+ B-
A+ B+ AB+
A- B- AB-
A+ B+ AB+
A- B- AB-
A+ B+ AB+
A- B- AB-
A+ B+ AB+
A- B- AB-
A+ B+ AB+
A- B- AB-
A+ B+ AB+
A- B- AB-
B
l
o
o
d
T
r
a
n
s
f
u
s
i
o
n
Dr. Tahir
Abbas
BalochDr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch
DD dd
D d
Dd
+
-
+
+
+
+
anti-Rho(D) test / positive
Dd dd
D, d d
DD, Dd
+
AA : XO M
AA : XY M
AAA : XY Metamale, Suppermale
AA : XX F
AA : XXY F
AA : XXX F
Sex Determination in Drosophila
X : A Genic Ratio System
Humans: XXY = M
Humans: XO = F
Ascaris contains 24 bivalent chromosomes,
n = 24 male 19 bivalents and 5 univalent.
Male 35 , Autosome 13 pairs AA , X = 8, Y
Gametes 13A + 8X, 13A + Y
Female 42, Autosome 13 pairs AA, X = 16
Gametes 13A + 8X XO in Drosophila is sterile male
XO in Man is Turner Syndrome is sterile Female
XO in Bug is Fertile Male
Drophila male genotype XY, XO
AA : XO M (sterile male)
AA : XY M
AAA : XY Metamale, Sterile, Suppermale
AA : XX F
AA : XXY F (fertile female)
AA : XXX F (Metafemale)
Metafemale A metafemale is a low viability Drosophila fruit
fly with a female phenotype in which the ratio of X
chromosomes to sets of autosomes exceeds .
Metamale (supermale) is a low viability Drosophila fruit fly
with a male phenotype in which the ratio of X chromosomes
to sets of autosomes (A) is less than 0.5.
COMPOUND SEX CHROMOSOMES
DimorphicBivalent is one pair of chromosomes in a tetrad,
with crossing over.
Univalent chromosome that is unpaired in
prophase 1 and not show crossing.
These are POLYPLOIDS with odd numbers
of chromosome sets.
Dr. Tahir Abbas Baloch
What is gene ?
What is gene action?
What is gene action in Neurospora?
What is gene action in Man?
What is one gene one enzyme ?
What is one gene one protein ?
What is one gene polypeptide?
Prove that proper position of amino acids is essential for proper activity of proteins ?
Write the genetic base/reason of sickle cell anemia?
Why Neurospora was selected for genetic experiments?
Dr. Tahir Abbas Baloch
E1
B C D ProductA
E2 E3 E4
1 2 3
PRECURSOR
NEGATIVE/FEED BACK INHIBITOR
Gene Gene Gene
Chromosome
On
Off
AutomaticSwitch
Water
Flow
A B CAll Ok
Fault Ok
Fault Fault
Ok
Fault Fault Fault
Dr.TahirAbbasBaloch
NEUROSPORA
Ascomycetes
Ascosporangium/Ascus
8 - Ascospores
X-Rays
Non-Mutant/Normal Grow in Minimal Medium
Vitamins, Sugar, Salt, Water
LETHAL DEAD SPORE NEVER GROW
Mutant Culture Not Grow in Minimal
Medium
SUPPLEMENTS ?
Carbohydrates Nucleic
Acids
Lipid Amino Acids- Proteins Arginine
Histidine
Proline
Methionine
Leucine
Tryptophan
Valine
Lysine
Glycine
Arginine
Ornithine Citruline Arginine
1 2 3 4 5 6
Beta – Normal Chain
F- Mutant Chain
energy
35
5
5
3
need “primer” bases to add on to
energy
energy
energy
3
no energy
to bond
energy
energy
energy
ligase
3 5

REPLICATION OF DNA
single-stranded binding proteins replication fork
helicase
leading strand
lagging strandleading strand
5
3
3
5
5
3
5
3
growing
replication fork
growing
replication fork
5
5
5
5
lagging strand
5 3
DNA polymerase III5
5
5
3
3
3
5
3
5
3 5 3
growing
replication fork
primase
RNA
5
5
5
5
3
3
3
3
growing
replication fork
DNA polymerase I
RNA
ligase
DNA polymerase III5
5
5
5
3
3
3
3
growing
replication fork
DNA polymerase I
RNA
Chromosome erosion
telomerase
5
5
5
3
3
3
3
growing
replication fork
TTAAGGGTTAAGGG
chromosomes get shorter
with each replication 5
Dr. Tahir Abbas Baloch
N 14
Cesium
Chloride
MESELSON & STAHL EXPERIMENT
N 14
N 15
N 15
N 15 N 15 N 15
N 14 N 14 N 14
t~ 0 sec T= 20 Mint T= 40 Mint
REPLICATION
OF
DNA
Dr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch
Non Template or Coding Strand or Sense Strand
Anti-Coding Strand or Anti - Sense Strand or Template Strand
A T C G A A T T C C C G G
T A G C T T A A G G G C C
A T C G
T A G C
A U C G A A U U C C C G G
A U C G
Sense code, Non sense Code, Codon
DNA
DNA
m RNA
Anti sense Code – t RNA
Decode
A U C
U A G
t RNA
(m RNA)
(t RNA)
5
3
3’ 5’
Towards 5' end of the sense strand is upstream
Towards 5' end of the RNA is upstream.
5’
3’
5’ 3’
TRANSCRIPTION
Dr. Tahir Abbas Baloch
Bacteria, sequence at -35 has TTGACA sequence
Promoter
PROMOTER SEQUENCE PROKARYOTES EUKARYOTES
TATAAT (TATA box) -10 -25
TTGACA (CAAT box) -35 -75, -70
Transcription of DNA begins for a particular gene (i.e., positions
upstream are negative numbers counting back from -1
Telomeres is TTAGGG
Palindromic Sequence is ACCTAGGT
Towards 5' end of the sense strand/Coding is upstream
Towards 5' end of the RNA is upstream.
Towards 3‘ end of the template strand is upstream
Dr. Tahir Abbas Baloch
DNA
Promotor
TTGACA-- TAC ACC GGT TAA ATC
AUG UGG CCA AUU UAG
m-RNA
15 Nucleotides
5 Genetic codes
4 Amino acids
Tripeptide Genetic codes = Amino acids
(without stop code)
Genetic codes = Amino acids +1
(with stop code)
21 Nucleotide
TRANSCRIPTION
Dr. Tahir Abbas Baloch
mRNA
5’
Leader Sequence
Leader RNA
AUG
Start Code
UAG
60 S
40 S
80 S
APE
1 Amino acyl t RNA ase Amino Acid
Charge t RNA
Methionine1
Anticode
Initiating Factor
5’
AUG UAG
APE
2 Amino acyl t RNA ase
2 Amino Acid
Charge t RNA
1
Initiating Factor
UAC
UAC
Decoding
2 Charge t RNA
2 Amino Acid
TRANSLATION
INITIATION
Dr. Tahir Abbas Baloch
5’
AUG UAG
APE
3 Amino acyl t RNA ase
3 Amino Acid
Charge t RNA
1
Initiating Factor
UAC
Decoding
3 Charge t RNA
3 Amino Acid2 Charge t RNA
Peptide Bond
2
5’
AUG UAG
APE
n Amino acyl t RNA ase
n Amino Acid
Charge t RNA
1
Initiating Factor n Charge t RNA
n Amino Acid
Peptide Bond
2 Stop Code
Elongation Factor
1
3
2
3
ELONGATION
Dr. Tahir Abbas Baloch
5’
AUG UAG
APE
n Amino acyl t RNA ase
1
Initiating Factor
Peptide Bond
2 Stop Code
Elongation Factor
2
3
3
4
APE
1
Free Polypeptide
n
n
n
Releasing Factor
TERMINATION
UAG
UAA
UGA
Stop Code
Non Sense
3
Total Genetic Codes 4
n
Total Genetic Codes 64
Total Sense Codes 61
Start Code AUG
Dr. Tahir Abbas Baloch
Adenine
C5H5N5
Purine
C5H4N4
Guanine
C5H5N5O
CytosinePyrimidine Thymine Uracil C5H4N4O3
Uric Acid
1. The ___________ is considered as father of genetics.
a) Darwin c) Lamarck d) Malthusb) Mendel
1. The ___________ is considered as father of nucleic acid.
a) Darwin b) Mendel c) Lamarck
1. The physical and structural unit of inheritance is _________
a) DNA b) Chromosome c) Gene d) Nucleotide
The __________ is the basic unit of chromosome and chromatin.
a) Nucleotide b) Nucleosome c) Gene d) Genome
The genetic code is AUG, then its decoding anticode is ________
a) TAC b) ATC c) UAC d) UAG
Which of the following is a correct definition of genetics?
Transmission of traits from parent to offspring
Study of variation between members of a species
Study of DNA Genes and traits determined by genes
If coding strand is AATTCCGCTA then mRNA will be __
a) AAUUCCGCTA b) TTUUGGCGAT c) AAUUCCGCUA
d) TTAAGGCGAT
1. The _____________ store information for protein synthesis.
DNA Cytoplasm r RNA t RNA
d) Friedrich Miescher
Dr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch
Dr. Tahir Abbas Baloch
Fingers / Toes = 5
Bone in Left Palm = 5
Bone in Right Palm = 5
Bone in Left Sole = 5
Bone in Right Sole = 5
Lumbar Vertebrae = 5
Kingdoms of Bio = 5
Prime End of DNA = 5
Head of m RNA = 5
Common Senses = 5
3rd Prime Number = 5
Starfish Appendages = 5
Pentemerous Petals = 5
Pentadactyl = 5
Lobes of Lungs = 5
Adenine = C5H5N5
Pillar of Islam = 5
Namaz/Azan = 5
Panjantan e Pak = 5
Ribs on Left Side = 12
Ribs on Right Side = 12
Thoracic Vertebrae = 12
Pair of Cranial Nerves = 12
Light Reactions = 12
Water Used = 12
Non Cyclic Reactions = 12
ATP Cyclic Reactions = 12
NADH Cyclic Reactions = 12
Photolysis = 12
O atom produced = 12
Duodenum length inch = 12
One foot have inches = 12
Heart Chakra Petals = 12
Max wind speed hurricane = 12
Months of Year = 12
Footballs Players = 12
Hours = 12
Imam = 12
Sure-e-Yusuf = 12
Facial Bones of Man = 14
Phalanges of Left Hand = 14
Phalanges of Right Hand = 14
Phalanges of Left Foot = 14
Phalanges of Right Foot = 14
Pea Plant Chromosomes = 14
Woodlouse legs = 14
Catalan Number = 14
Companion Pell Number = 14
Atomic Wt of Nitrogen = 14
Electrons of f = 14
Holy Helpers = 14
Muqatta (Quran) = 14
Masoomen (Infallibles) = 14
5 12
14
Dr. Tahir Abbas Baloch

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Genetic Competition Exam

  • 1. Dr. Tahir Abbas Baloch Ph.D. Zoology (Punjab University, Lahore) tahirabbasbloch@gmail.com 03339810179 Dr. Tahir Abbas Baloch You Tube https://www.youtube.com/channel/UC- 9mb8kyILhPKKl9PwAdvbw?view_as=subscriber Previous Lecture Genetics: 10, 11,12 Genetics, Mendelian Inheritance Dr. Tahir Abbas Baloch
  • 2. Dr. Tahir Abbas Baloch You Tube https://www.youtube.com/channel/UC- 9mb8kyILhPKKl9PwAdvbw?view_as=subscriber Dr. Tahir Abbas Baloch MDCAT, NUMS, PPSC 13
  • 3. A A B b HOMOZYGOUS HETEROZYGOUS HETEROZYGOUS (AABb) A A bB A A bB A A bB Un – replicated Replicated ALLELES NOT- ALLELES NOT- ALLELES Bacteria, have a single ring of DNA, have one allele per gene per organism Chiasma/Synaptonemal Complex Synapse/Synapsis Centromere Primary Constriction Sec Constriction Junk Gene –non coding/Heterochromatin/Euchromatin TELOMERS TELOMERS Dr. Tahir Abbas Baloch coupling/cisphase trans phase REPULSION PHASE Crossing Over Recombinants *Coupling Reaction Sister Chromatids Daughter Strands Replicated Forms transposonorjumpinggene
  • 4. GENES A, B, D, E, F, H, I, R, T, W, X, Y, Z, Rh, SRY, W+…… Dominant Genes a, b, d, e, f, h, i, r, t, w, X, Y, Z, Rh, SRY, ……….…… Recessive Genes AA, BB, DD, EE, FF, HH, II, RR, TT, W+ W+, XX, YY, ZZ, …… Allele Aa, Bb, Dd, Ee, Ff, Hh, Ii, Rr, Tt, W+ w, Xx, Yy, Zz, …………. Allele aa, bb, dd, ee, ff, hh, ii, rr, tt, w w, xx, yy, zz, ………………... AlleleALLELES SINGLE ALLES AA1A2a, BB1b, CC1C2C3, D1D2D3D4 -------------- AaBbCc ---------------------------------------------------- POLYGENES/ Polygeny/Polygenic AA, Aa, aa, A1A1, AA2, A1A2, AA1 A1a, A2a Gene Pool of Populations A, A1, A2, a 4 Individual = 9 Genes= 2n= 18 AA, Aa, aa, A2A1, AA1, A1A1, AA1 A1a, aA1 Gene Flow A2 AA A2A1 Bottle Neck Effects Genetic Drift AA, AA2, A2A2, A1A1, AA2, A1A2, AA1, A1A, A2A, AA, AA2, A2A2, A1A1, AA2, A1A2, AA1, A1A, A2A, A1A2, AA1 A, A1, A2 3 Population Density PopulationPressure A Gene a A1 A2 Alles Mutation Genetic Diversity Gene Pool DeclineDr. Tahir Abbas Baloch DOUBLE ALLES MULTIPLE ALLES
  • 5. GENE:  unit of heredity transferred from parent to offspring.  sequence of nucleotides in DNA /RNA determines order of monomers in polypeptide  basic physical and functional unit of heredity.  unit of hereditary information that occupies a fixed position on a chromosome.  bacteria gene is more complex than eukaryotes.  plasmids, contain (usually) non-essential genes.  viral genes are mostly present in a single molecule of nucleic acid.  Females have more genes & more complex genome than male due to XX  LOCUS is a position of genes on a chromosome. GENE MARKER (Probe) DNA MARKER Molecular Marker Morphological Marker  Transposable element (transposon, or jumping gene) DNA sequence that can change its position within a genome.  Gene silencing is regulation of gene in a cell to prevent their expression. LOCUST Dr. Tahir Abbas Baloch  Wilhelm Johannsen coined the word “ GENE "
  • 6. Wilhelm Johannsen describe the Mendelian units of heredity (gene), expressed as phenotype and genotype. Mendel is Considered Father of Genetics SelfingDusting Dr. Tahir Abbas Baloch
  • 7. Mendel First Law Mendel Law of Inheritance Law of Dominance Monohybrid Law Law of Purity of Gametes Phenotypic Ratio 3: 1 Genotypic Ratio 1: 2: 1 Genotypic Ratio of Homozygous Dominant 1/4 Genotypic Ratio of Heterozygous 2/4 = ½= 0.5 Genotypic Ratio of Homozygous Recessive 1/4 Dr. Tahir Abbas Baloch
  • 8. P 1 F 1 F 2 F 3Dr. Tahir Abbas Baloch
  • 9. Dr. Tahir Abbas Baloch Mendel First Law Round = ¾ Yellow = ¾ Wrinkle = ¼ Green = ¼ Product Rule Round x Yellow = ¾ x ¾ = 9/16 Round x Green = ¾ x ¼ = 3/16 Wrinkle x Yellow = ¾ x ¼ = 3/16 Wrinkle x Green = ¼ x ¼ = 1/16Tall = ¾ Short = ¼ Round x Tall = ¾ x ¾ = 9/16 Round x Short = ¾ x ¼ = 3/16 Wrinkle x Tall = ¾ x ¼ = 3/16 Wrinkle x Short = ¼ x ¼ = 1/16 The probability of two or more independent events are equal to the product of their individual probabilities.
  • 10. 1 : 2 : 2 : 4 : 1 : 2 : 1 : 2 : 1 genotypes = 9 Total = 169 : 3 : 3 : 1 phenotypes = 4 F 2 F 1 P 1 LAW OF INDEPENDENT ASSORTMENT Male gametes 4 Femalegametes4 4 x 4 = 16 Meiosis Gametogenesis ½ 0.5 DihybridSelf Cross F1 x F1
  • 11. AaBb Dihybrid aabb Recessive AB, Ab, aB, ab Gametes ab Gametes AaBb, Aabb, aaBb,aabb 1 : 1 : 1 : 1 P 1 F 1 Diallelic Characters Dihybrid Characters Test Cross Complete Dominance Law of Independent Assortment Single Crossing Over Recombination Dr. Tahir Abbas Baloch
  • 12. AABBCC Dark Red aabbcc White ABC Gametes abc Gametes AaBbCc P 1 F 1 Triallelic Characters Trihybrid Characters Test Cross Polygeny Continuously varying Traits Recombination Dr. Tahir Abbas Baloch Trihybrid Light Red Incomplete Dominance Wheat grain color
  • 13. AaBbCc Light Red AaBbCc F 1 x F1 Meiosis Gametes ABC ABc AbC Abc 2 n aBC aBc abC abc ABC ABC ABc ABc AbC AbC Abc Abc aBC aBC aBc aBc abC abC abc abc ABC ABC ABC ABc ABC AbC ABC Abc ABC aBC ABC aBc ABC abC ABC abc ABc ABC ABc ABc ABc AbC ABc Abc ABc aBC ABc aBc ABc abC ABc abc AbC ABC AbC ABc AbC AbC AbC aBC AbC aBc AbC abC AbC abc AbC Abc Abc ABC Abc ABc Abc AbC Abc aBC Abc aBc Abc abC Abc abc Abc Abc aBC ABC aBC ABc aBC AbC aBC aBC aBC aBc aBC abC aBC abc aBC Abc aBc ABC aBc ABc aBc AbC aBc aBC aBc aBc aBc abC aBc abc aBc Abc abC ABC abC ABc abC AbC abC aBC abC aBc abC abC abC abc abC Abc abc ABC abc ABc abc AbC abc aBC abc aBc abc abC abc abc abc Abc 6 012 11 1 1 1 22 2 2 2 2 2 22 2 5 5 4 5 4 4 3 5 4 4 3 4 3 3 5 4 4 3 4 3 3 2232334 5 4 4 3 4 3 3 4 3 3 2 3 3334 3 Dose Genotypes Phenotypes Ratio 0 aabbcc White 1/64 1 Aabbcc (2) aaBbcc (2) aaBbcc (2) 6/64 AABBC AABCC AABC ABBCC ABBC ABCC ABC AABBC AABB AABC AAB ABBC ABB ABC BC AABCC AABC AACC ABCC ABC ACC ACACC AABC ABC AAC ABC AB ACAA ABBCC ABBC ABBC BBCC BBC BCC BCABC ABBC ABB ABC BBC BB BCAB ABCC ABC ACC BCC BC CCAC ABC AB AC BC 2 AA(1) A B C 0BB AB (3) AC (4)BC(5)BB(1) CC(1) 15/64 3 BCC (2)ACC (2) ABC (9) ABB (2) BBC (2) AAC (2)AAB (1) 20/64 4 ABCC (3) ABBC (5) BBCC (1) AABC (4)AACC (1) AABB (1) 15/64 5 ABBCC (2) AABCC (2) AABBC (2) 6/64 6 AABBCC 1/64 1 3 6 7 6 3 1 Light Pink Pink Light Red Red Moderate Dark Red Dark Red White = 1/64 Light Pink = 3/32 Pink = 15/64 Light Red = 5/16 Red = 15/64 Moderate = 3/32 Dark Red = 1/64 7 Designed by; Dr. Tahir Abbas F2
  • 14. Ratio, Proportion, Probability Monohybrid First Law Phenotype in F 2 is 3:1, Genotypic 1:2:1 Dihybrid/Second Law Phenotype 9:3:3 :1, Genotype 1:2:2:4:1:2:1:2:1 Incomplete Dominance Phenotype & Genotype 1:2:1 Trihybrid/Polygeny Phenotype 1:6:15:20:15:6:1 Monohybrid First Law, Phenotype in F 1 is 1:1:1:1 Dihybrid Second Law, Phenotype in F 1 is 1:1:1:1 Trihybrid Second Law, Phenotype in F 1 is 1:1:1:1 Test Cross for Homozygous is always 100% Test Cross for Heterozygous is # 100% but not more than 50 %
  • 15. T t Aa Bb Rr Yy Complete Dominance Dominant 100 % in F1, Recessive in F 1 0 % R1 R2 R1R2 In Complete Dominance Dominant 50 % in F1, Recessive in F 1 50 % A B AB Dominant 100 % in F1, Recessive in F 1 100 % Co-Dominance A a Traits - I Traits - II Pleiotropy Chromosome 9 Chromosome 19 EPISTASIS Non-Homologous Dominance Polygeny/Contineously Varying Traits AaBbCc /Trihybrids F 1 F 2 1:6:15:20:15:6:1 Phenotypes = 7 Multiple Allele Blood Group Gene ABO Dr. Tahir Abbas Baloch
  • 16. Dr. Tahir Abbas Baloch Sutton and Boveri, states chromosomes are the vehicles of genetic heredity; beave as segregation, independent assortment, and linkage. Chromosomal Theory Gene theory - genes are the basic units in which characteristics are passed from one generation to the next. Neutral Gene theory, the behavior of mutant genes in populations is determined by random genetic drift/bottle neck effect. Thomas Hunt Morgan won the Nobel Prize in 1933 prove that rule by Drosophila.
  • 17. LINK Gene to Gene Linkage Gene to Chromosome Link Genetic Linkage Linkage Group Homologous Pairs Genes of Chromatid Autosomal Linkage Sex Linked Genes Male = Female Male # Female X-Linked Genes Y-Linked Genes Only in MaleX-Linked Dominant Genes X-Linked Recessive GenesMale ≥ Female Female ≥ Male Pseudoautosomal Gene on mammalian X and Y chromosomes e.g. Mammary Gland Genes Hemophilia Red-green Blindness White Eye Color in Drosophila Baldness Hypophosphatemic ricket Blood Group Gene
  • 18. X X W+ W+ Female Red Eye X X W+ w Hetrozygous, Carrier Female Red Eye X Y W+ Male Red Eye X X w w Female White Eye X Y w Male white Eye X X X , X Eggs X Y X , Y Sperms HOMOGAMETIC HETROGAMETIC WILD TYPE (Red Eye) AUTOSOME A = 2n-2 Sex Chromosome ALLOSOMES
  • 19. X X W+ W+ Female Red Eye X X W+ w Female Red Eye X X w w Female White Eye X Y W+ Male Red Eye X Y w Male White Eye Female/Male Red Eye Female/Male Red Eye 2 Female/1 Male Red Eye 2 Female Red Eye 2 Male White Eye 1 Male White Eye Female/Male Red Eye Female / Male White Eye Female / Male White Eye Parental Cross Test Cross Test Cross Back Cross Reciprocal Cross Confirmatory Cross Self Cross = F 1 x F 1 = F 2 x F 2 X X W+ W+ Female Red Eye X X W+ w Hetrozygous, Carrier Female Red Eye X Y W+ Male Red Eye X X w w Female White Eye X Y w Male white Eye Dr. Tahir Abbas Baloch
  • 20. X X W+ w Hetrozygous, Carrier Female Red Eye X Y w Male white Eye X X W+ W+ Female Red Eye X Y w Male white Eye Parental Cross P 1 X , X W+ W+ X , Y w X X W+ w Hetrozygous, Carrier Female Red Eye X Y W+ Male Red Eye SelfCrossF1xF1 X , X W+ w X , Y W+ X X W+ W+ Female Red Eye X Y W+ Male Red Eye X X W+ w Hetrozygous, Carrier Female Red Eye F 2 X X W+ w Hetrozygous, Carrier Female Red Eye X Y w Male White Eye TEST CROSS X , X W+ w X , Y w Gametes by Meiosis X X W+ w Hetrozygous, Carrier Female Red Eye X Y W+ Male Red Eye X X w w Female White Eye X Y w Male White Eye X Y w Male White Eye RECIPROCAL CROSSX X w w Female White Eye X Y W+ Male Red Eye X , X w w X , Y W+ P1 = F(R) x M (W) → 2 F (R,C), 2 M (R) Parental Cross 2:2, 1: 1, 100 % F1xF1 = F(R,C) x M (R) → 1 F (R,C), 1 M (R) Self Cross 1 F (R) , 1 M (W) 3:1 (R,W)/75%, 25%, 100% (F), 50% (M) Test Cross = F(R,C) x M (W) → 1 F (R,C), 1 M (R) 1 F (W) , 1 M (W) 2:2 (R,W) or 1: 1 50% (F), 50% (M) Reciprocal Cross = F(W) x M (R) → 2 F (R,C), 2 M (W) 1:1 (R,W) or 50%, 100% (F), 100% (M white) One gene one enzyme One gene polypeptide Sex Determination Chromosomal Theory Gene Theory Sutton Theory T.H Morgan Work X-Linked Recessive Trait Page Prepared By Dr. Tahir Abbas Baloch F 1
  • 21. X X W+ W+ Female Red Eye X Y w Male White Eye Gametes X , X W+ W+ X , Y w X X W+ w Female Red Eye Carrier X Y W+ Male Red Eye 2 F (R) : 2 M (R) 1 : 1 overall 100% Red eyes ParentalCross Reciprocal Cross Phenotypically inverse to P1 Male Red Eye overall 50% Red eyes Female 100% Red Male 100 % White Eye Test Cross Test Cross
  • 22. X X H h Heterozygous, Carrier Female X Y h Hemophiliac Male X X H H Normal Female X Y h Hemophiliac Male Parental Cross P 1 X , X H H X , Y h X X H h Heterozygous, Carrier Female X Y H Normal Male X , X H h X , Y H X X H H Normal Female X Y H Normal Male X X H h Heterozygous, Carrier Female F 2 X X H h Heterozygous, Carrier Female X Y h Male Hemophiliac TEST CROSS X , X H h X , Y h Gametes by Meiosis X X H h Heterozygous, Carrier Female X Y H Normal Male X X h h F Hemophiliac X Y h M Hemophilic X Y h Male Hemophiliac RECIPROCAL CROSSX X h h Female Hemophiliac X Y H Normal Male X , X h h X , Y H P1 = F(N) x M (H) → 2 F (N,C), 2 M (H) Parental Cross 2:2, 1: 1, 100 % F1xF1 = F(N,C) x M (N) → 1 F (N,C), 1 M (N) Self Cross 1 F (N) , 1 M (H) 3:1 (N,H)/75%, 25%, 100% (F), 50% (M) Test Cross = F(N,C) x M (H) → 1 F (N,C), 1 M (N) 1 F (H) , 1 M (H) 2:2 (N,H) or 1: 1 50% (F), 50% (M) Reciprocal Cross = F(H) x M (N) → 2 F (N,C), 2 M (H) 1:1 (N,H) or 50%, 100% (F), 100% (M H) One gene one enzyme One gene polypeptide Sex Determination Chromosomal Theory Gene Theory Sutton Theory T.H Morgan Work X-Linked Recessive Trait Page Prepared By Dr. Tahir Abbas Baloch F 1
  • 23. Gene recombination is naturally occurring during meiosis in eukaryotes, involves the pairing of homologous chromosomes and information transfer between the chromosomes. A B a b AB ab Ab aB AB ab Non-Parental α Crossing Over α Recombination α Genetic Map α Gene Distance A B 20 A B 20 C 15 35 A B 15 A B 15 C ? 55 ? = 40 A B a b A B a b A B Synapsis Chiasma A B B a AB aB a bb A Ab ab NP (recombinant) P P a b a b ab P AB aB Ab ab abAa Bb aa Bb Aa Bb aa bb PP NP NP Recombinant Frequency = Σ Non-Parental x 100 = Genetic Map Total 4x1=4 P = 40+40=80 NP = 10+10=20
  • 24. A B AB O IA IA IA i IB IB IB i IA IB ii + DD Dd _ dd + DD Dd _ dd + DD Dd _ dd + DD Dd _ dd PHENOTYPES GENOTYPE (ABO)GENOTYPE (ABO-Rh) 12 6 4 Dr. Tahir Abbas Baloch
  • 25. DD Dd dd DDDddd + +, - - + + _ + + _ FATHER MOTHER OFFSPRINGS DD + Dd + dd _ + -h h Dr. Tahir Abbas Baloch + +- + +, - -+ +- + + +
  • 26. I I A A I i A I I B B I i B I I A B i i H H H h h h A A O B B O AB AB O O O O 9 19 EPISTASIS BOMBAY PHENOTYPE ABO Group
  • 27. I I A A I i A I I B B I i B I I A B i i9 I I A A DD Dd dd DD Dd dd DD Dd dd DD Dd dd DD Dd dd DD Dd dd I i A I I B B I i B I I A B i i 1 DD,Dd,dd DD,Dd,dd DD,Dd,dd DD,Dd,dd DD,Dd,ddDD,Dd,dd Dr. Tahir Abbas Baloch
  • 28. i O A B AB O A B AB O + A - A+ A+ AB+B - B+ AB - AB+ AB- B - B+ A -O - O + O - ii, dd, hh ii, DD, Dd, HH, Hh IAIA, IAi, dd, Hh, hh IAIA, IAi, DD, Dd, Hh, hh IBIB, IBi, dd, Hh, hh IBIB, IBi, DD, Dd, Hh, hh IAIB, dd, Hh, hh IAIB, DD, Dd, Hh, hh Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes YesYesYes Yes Yes Yes A+ A- O+ O- O- O+ O- A- O- A+ A- O+ O- B- O- B+ B- O+ O- A- B- A+ A- B- O+ O- O+ A- O- A+ O+ O- A+ A- O+ O- B+ B+ O+ B- O- B+ O+ B- O- A+ B+ A- B- A+ B+ A- B- A- O- A+ O+ A- O- A- O- A+ O+ A- O- A- B- AB- O- A+ B+ AB+ O+ A- B- AB- O- A- B- AB- A+ B+ AB+ A- B- AB- A+ O+ A- O- A+ O+ A- O- A+ O+ A- O- A+ B+AB+O+ A- B-AB- O- A+ B+AB+ O+ A- B- AB- O- A+ B+ AB+ A- B- AB- A+ B+ AB+ A- B- AB- B- O- B+ O+ B- O- A- B- AB- O- A+B+AB+O+ A-B-AB-O- B- O- B+ O+ B- O- A- B- AB- A+ B+ AB+ A- B- AB- B+ O+ B- O- B+ O+ B- O- A+B+AB+ O+ A- B- AB- O- A+B+AB+ O+ A- B- AB- O- B+ O+ A- O- B+ O+ A- O- A+ B+ AB+ A- B- AB- A+ B+ AB+ A- B- AB- A- O- A+ B+ A- B- A- B- AB- A+ B+ AB+ A- B- AB- A- B- AB- A+ B+ AB+ A- B- AB- A- B- AB- A+ B+ AB+ A- B- AB- A+ A- B+ B- A+ A- B+ B- A+ B+ AB+ A- B- AB- A+ B+ AB+ A- B- AB- A+ B+ AB+ A- B- AB- A+ B+ AB+ A- B- AB- A+ B+ AB+ A- B- AB- A+ B+ AB+ A- B- AB- B l o o d T r a n s f u s i o n Dr. Tahir Abbas BalochDr. Tahir Abbas Baloch
  • 29. Dr. Tahir Abbas Baloch DD dd D d Dd + - + + + + anti-Rho(D) test / positive Dd dd D, d d DD, Dd +
  • 30. AA : XO M AA : XY M AAA : XY Metamale, Suppermale AA : XX F AA : XXY F AA : XXX F Sex Determination in Drosophila X : A Genic Ratio System Humans: XXY = M Humans: XO = F
  • 31. Ascaris contains 24 bivalent chromosomes, n = 24 male 19 bivalents and 5 univalent. Male 35 , Autosome 13 pairs AA , X = 8, Y Gametes 13A + 8X, 13A + Y Female 42, Autosome 13 pairs AA, X = 16 Gametes 13A + 8X XO in Drosophila is sterile male XO in Man is Turner Syndrome is sterile Female XO in Bug is Fertile Male Drophila male genotype XY, XO AA : XO M (sterile male) AA : XY M AAA : XY Metamale, Sterile, Suppermale AA : XX F AA : XXY F (fertile female) AA : XXX F (Metafemale) Metafemale A metafemale is a low viability Drosophila fruit fly with a female phenotype in which the ratio of X chromosomes to sets of autosomes exceeds . Metamale (supermale) is a low viability Drosophila fruit fly with a male phenotype in which the ratio of X chromosomes to sets of autosomes (A) is less than 0.5. COMPOUND SEX CHROMOSOMES DimorphicBivalent is one pair of chromosomes in a tetrad, with crossing over. Univalent chromosome that is unpaired in prophase 1 and not show crossing. These are POLYPLOIDS with odd numbers of chromosome sets.
  • 32. Dr. Tahir Abbas Baloch What is gene ? What is gene action? What is gene action in Neurospora? What is gene action in Man? What is one gene one enzyme ? What is one gene one protein ? What is one gene polypeptide? Prove that proper position of amino acids is essential for proper activity of proteins ? Write the genetic base/reason of sickle cell anemia? Why Neurospora was selected for genetic experiments?
  • 33. Dr. Tahir Abbas Baloch E1 B C D ProductA E2 E3 E4 1 2 3 PRECURSOR NEGATIVE/FEED BACK INHIBITOR Gene Gene Gene Chromosome On Off AutomaticSwitch Water Flow A B CAll Ok Fault Ok Fault Fault Ok Fault Fault Fault
  • 34. Dr.TahirAbbasBaloch NEUROSPORA Ascomycetes Ascosporangium/Ascus 8 - Ascospores X-Rays Non-Mutant/Normal Grow in Minimal Medium Vitamins, Sugar, Salt, Water LETHAL DEAD SPORE NEVER GROW Mutant Culture Not Grow in Minimal Medium SUPPLEMENTS ? Carbohydrates Nucleic Acids Lipid Amino Acids- Proteins Arginine Histidine Proline Methionine Leucine Tryptophan Valine Lysine Glycine Arginine Ornithine Citruline Arginine
  • 35. 1 2 3 4 5 6 Beta – Normal Chain F- Mutant Chain
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  • 37. energy 35 5 5 3 need “primer” bases to add on to energy energy energy 3 no energy to bond energy energy energy ligase 3 5 
  • 38. REPLICATION OF DNA single-stranded binding proteins replication fork helicase leading strand lagging strandleading strand 5 3 3 5 5 3 5 3 growing replication fork growing replication fork 5 5 5 5 lagging strand 5 3
  • 39. DNA polymerase III5 5 5 3 3 3 5 3 5 3 5 3 growing replication fork primase RNA 5 5 5 5 3 3 3 3 growing replication fork DNA polymerase I RNA ligase
  • 40. DNA polymerase III5 5 5 5 3 3 3 3 growing replication fork DNA polymerase I RNA Chromosome erosion telomerase 5 5 5 3 3 3 3 growing replication fork TTAAGGGTTAAGGG chromosomes get shorter with each replication 5
  • 41. Dr. Tahir Abbas Baloch N 14 Cesium Chloride MESELSON & STAHL EXPERIMENT N 14 N 15 N 15 N 15 N 15 N 15 N 14 N 14 N 14 t~ 0 sec T= 20 Mint T= 40 Mint REPLICATION OF DNA
  • 42. Dr. Tahir Abbas Baloch
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  • 44. Dr. Tahir Abbas Baloch Non Template or Coding Strand or Sense Strand Anti-Coding Strand or Anti - Sense Strand or Template Strand A T C G A A T T C C C G G T A G C T T A A G G G C C A T C G T A G C A U C G A A U U C C C G G A U C G Sense code, Non sense Code, Codon DNA DNA m RNA Anti sense Code – t RNA Decode A U C U A G t RNA (m RNA) (t RNA) 5 3 3’ 5’ Towards 5' end of the sense strand is upstream Towards 5' end of the RNA is upstream. 5’ 3’ 5’ 3’ TRANSCRIPTION
  • 45. Dr. Tahir Abbas Baloch Bacteria, sequence at -35 has TTGACA sequence Promoter PROMOTER SEQUENCE PROKARYOTES EUKARYOTES TATAAT (TATA box) -10 -25 TTGACA (CAAT box) -35 -75, -70 Transcription of DNA begins for a particular gene (i.e., positions upstream are negative numbers counting back from -1 Telomeres is TTAGGG Palindromic Sequence is ACCTAGGT Towards 5' end of the sense strand/Coding is upstream Towards 5' end of the RNA is upstream. Towards 3‘ end of the template strand is upstream
  • 46. Dr. Tahir Abbas Baloch DNA Promotor TTGACA-- TAC ACC GGT TAA ATC AUG UGG CCA AUU UAG m-RNA 15 Nucleotides 5 Genetic codes 4 Amino acids Tripeptide Genetic codes = Amino acids (without stop code) Genetic codes = Amino acids +1 (with stop code) 21 Nucleotide TRANSCRIPTION
  • 47. Dr. Tahir Abbas Baloch mRNA 5’ Leader Sequence Leader RNA AUG Start Code UAG 60 S 40 S 80 S APE 1 Amino acyl t RNA ase Amino Acid Charge t RNA Methionine1 Anticode Initiating Factor 5’ AUG UAG APE 2 Amino acyl t RNA ase 2 Amino Acid Charge t RNA 1 Initiating Factor UAC UAC Decoding 2 Charge t RNA 2 Amino Acid TRANSLATION INITIATION
  • 48. Dr. Tahir Abbas Baloch 5’ AUG UAG APE 3 Amino acyl t RNA ase 3 Amino Acid Charge t RNA 1 Initiating Factor UAC Decoding 3 Charge t RNA 3 Amino Acid2 Charge t RNA Peptide Bond 2 5’ AUG UAG APE n Amino acyl t RNA ase n Amino Acid Charge t RNA 1 Initiating Factor n Charge t RNA n Amino Acid Peptide Bond 2 Stop Code Elongation Factor 1 3 2 3 ELONGATION
  • 49. Dr. Tahir Abbas Baloch 5’ AUG UAG APE n Amino acyl t RNA ase 1 Initiating Factor Peptide Bond 2 Stop Code Elongation Factor 2 3 3 4 APE 1 Free Polypeptide n n n Releasing Factor TERMINATION UAG UAA UGA Stop Code Non Sense 3 Total Genetic Codes 4 n Total Genetic Codes 64 Total Sense Codes 61 Start Code AUG
  • 50. Dr. Tahir Abbas Baloch Adenine C5H5N5 Purine C5H4N4 Guanine C5H5N5O CytosinePyrimidine Thymine Uracil C5H4N4O3 Uric Acid
  • 51. 1. The ___________ is considered as father of genetics. a) Darwin c) Lamarck d) Malthusb) Mendel 1. The ___________ is considered as father of nucleic acid. a) Darwin b) Mendel c) Lamarck 1. The physical and structural unit of inheritance is _________ a) DNA b) Chromosome c) Gene d) Nucleotide The __________ is the basic unit of chromosome and chromatin. a) Nucleotide b) Nucleosome c) Gene d) Genome The genetic code is AUG, then its decoding anticode is ________ a) TAC b) ATC c) UAC d) UAG Which of the following is a correct definition of genetics? Transmission of traits from parent to offspring Study of variation between members of a species Study of DNA Genes and traits determined by genes If coding strand is AATTCCGCTA then mRNA will be __ a) AAUUCCGCTA b) TTUUGGCGAT c) AAUUCCGCUA d) TTAAGGCGAT 1. The _____________ store information for protein synthesis. DNA Cytoplasm r RNA t RNA d) Friedrich Miescher Dr. Tahir Abbas Baloch
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  • 62. Fingers / Toes = 5 Bone in Left Palm = 5 Bone in Right Palm = 5 Bone in Left Sole = 5 Bone in Right Sole = 5 Lumbar Vertebrae = 5 Kingdoms of Bio = 5 Prime End of DNA = 5 Head of m RNA = 5 Common Senses = 5 3rd Prime Number = 5 Starfish Appendages = 5 Pentemerous Petals = 5 Pentadactyl = 5 Lobes of Lungs = 5 Adenine = C5H5N5 Pillar of Islam = 5 Namaz/Azan = 5 Panjantan e Pak = 5 Ribs on Left Side = 12 Ribs on Right Side = 12 Thoracic Vertebrae = 12 Pair of Cranial Nerves = 12 Light Reactions = 12 Water Used = 12 Non Cyclic Reactions = 12 ATP Cyclic Reactions = 12 NADH Cyclic Reactions = 12 Photolysis = 12 O atom produced = 12 Duodenum length inch = 12 One foot have inches = 12 Heart Chakra Petals = 12 Max wind speed hurricane = 12 Months of Year = 12 Footballs Players = 12 Hours = 12 Imam = 12 Sure-e-Yusuf = 12 Facial Bones of Man = 14 Phalanges of Left Hand = 14 Phalanges of Right Hand = 14 Phalanges of Left Foot = 14 Phalanges of Right Foot = 14 Pea Plant Chromosomes = 14 Woodlouse legs = 14 Catalan Number = 14 Companion Pell Number = 14 Atomic Wt of Nitrogen = 14 Electrons of f = 14 Holy Helpers = 14 Muqatta (Quran) = 14 Masoomen (Infallibles) = 14 5 12 14 Dr. Tahir Abbas Baloch