The document discusses the characterization of graphene through various techniques. It begins with an introduction to the unique 2D crystal structure of graphene. It then discusses several characterization techniques used to study graphene's morphology, properties, defects, and layers, including Raman spectroscopy, SEM, TEM, XRD, UV-Vis spectroscopy, and AFM. Specifically, it describes how Raman spectroscopy can be used to study graphene layers and structural quality by analyzing peaks in the Raman spectrum. The document also briefly mentions some applications of graphene in areas like energy storage, water filtration, transistors, solar cells, and biomedicine.