MOTION MANAGEMANT IN LUNG SBRT BY DR KANHU CHARAN PATRO
synopsis
1. UTILITY OF WBC
SCATTERGRAM IN
LEUCOPENIA
DR.MALAVIKA G
DISSERTATION SYNOPSIS FOR THE COURSE
OF MD PATHOLOGY
GUIDE: DR.CHETHANA MANNEM
ASSOCIATE PROFESSOR, DEPARTMENT OF
PATHOLOGY
2. INTRODUCTION
• A basic hematological work up is often the first
investigation requested in any diseased condition.
• It forms the basis for decision-making regarding further
evaluation and management.
• With automation in hematology, accurate results are
available which are time and labour efficient
3. INTRODUCTION
• Automated Hematology Analyzers utilize light scatter
properties of WBCs to provide information on WBC
distribution patterns.
• Abnormal patterns in the scatterplots generated by these
instruments has been proven to be useful in the diagnosis
of many leukocytic disorders.
4. NEED FOR STUDY
• Automated hematology analyzers (AHA) flag blood
samples showing leucopenia, necessitating peripheral
blood smear examination, which is time-intensive and
skill-dependent. Further, leucopenic samples require
examination of multiple smears / buffy coat preparation
for adequate evaluation.
• On the contrary, AHA study large number of cells from
each blood sample and generate WBC scattergrams
providing a clue about the cause of leukopenia.
• Hence, the study proposes to evaluate the use of WBC
scattergrams in identifying the cause of leucopenia.
5. AIM AND OBJECTIVES
• To study the WBC scattergram patterns generated in
samples showing leucopenia.
• To compare the WBC scattergrams with peripheral
blood smear examination.
• To correlate the WBC scattergram patterns with
clinical and other relevant laboratory investigations.
6. MATERIALAND METHODS
• The study will be conducted in the Department of
Pathology, Central laboratory, KIMS, Bangalore.
• All EDTA anticoagulated blood samples showing
leucopenia (Total count <4000 cells/ cc) on automated
hematology analyzer Sysmex XN-1000 will be studied.
• For the WBC scattergrams generated, corresponding
peripheral blood smears (PBS) will be prepared and
examined.
• The patterns observed in scattergram will be compared with
PBS findings, clinical details and other relevant laboratory
investigations.
7. MATERIALS
• STUDY DURATION : 18 months.
• STUDY DESIGN: Observational study
• SAMPLE SIZE : 240
• SAMPLING METHOD : Purposive sampling
• INCLUSION CRITERIA : Blood samples showing leucopenia
on automated hematology analyzer will be included
• EXCLUSION CRITERIA : Leucopenia cases wherein clinical
details or peripheral blood smear is not available.
8. APPROACH TO ETHICALASPECTS
• The study is being conducted on clinician-indicated
hematology samples, hence requiring no additional
patient intervention.
• Patients confidentiality will be maintained.
