BioChain Company Overview


Published on

BioChain Company Overview

Published in: Business, Technology
  • Be the first to comment

  • Be the first to like this

No Downloads
Total views
On SlideShare
From Embeds
Number of Embeds
Embeds 0
No embeds

No notes for slide

BioChain Company Overview

  1. 1. BioChain Institute, Inc. Turn Ideas into Reality<br /> Bio-Sample Preparation for Life Science and Diagnostics<br />1<br />
  2. 2. Company Profile<br />Founded in 1994, Privately held<br />Group Companies :<br /> Biochain Institute, Inc in Hayward, CA, USA<br /> Biochain(Beijing) Science-Tech Corp. in Beijing, China<br /> Beijing Modern Gaoda Biotechnology Co. in Beijing, China <br />Branch over 35 distributors worldwide <br />About 200 employees around the world <br />Revenue has been growing annually in double digits.<br />Profitable since 1999 <br />Over ten thousand customers, industry and academic<br />Over 5000 products and providing various custom services<br />ISO 9001, 13485 Certified & GMP<br />2<br />
  3. 3. Facility<br />USA Facility<br />Location: 3517 Breakwater Avenue, Hayward, CA, USA<br />Size: 10,000 sq ft <br />Level: R&D and Manufacture facility <br />China Facility<br />Location: No.7A,Yongchang North Road, Economic and Technological Development Area, Beijing, China <br />Size: 80,000 sq ft manufacture<br />Level: Clinical Diagnostic GMP manufacture and laboratory <br />3<br />
  4. 4. Facility - USA<br />4<br />
  5. 5. Facility - USA<br />.<br />5<br />
  6. 6. Facility - China<br />6<br />
  7. 7. Company Culture<br />Respect<br />Integrity<br />Improvement<br />Innovation <br />Honor<br />7<br />
  8. 8. Team<br />Scientific Team<br /> 35 Ph.D. and over 40 Master’s Degree scientists who are well trained in the related research and technology development field. Many of them have over 10 years of working experience in the industry.<br />8<br />
  9. 9. Quality Policy<br /><ul><li>Business Growth.
  10. 10. Customers’ Satisfaction.
  11. 11. Quality Products.
  12. 12. Continuous Improvement. </li></ul>9<br />
  13. 13. Quality Certificate - USA<br />10<br />
  14. 14. Quality Certificate - China<br />11<br />
  15. 15. Business Scope<br />Biochain Institute Inc. USA<br /> Sample Preparation for Diagnostics & Life Science Research<br />Biochain(Beijing) Science-Tech Corp. <br /> Molecular Diagnostics and Central Lab<br />Beijing Modern Gaoda Biotechnology Co.<br /> Immunodiagnostics <br />12<br />
  16. 16. Product Category<br />DNA <br />cDNA<br />RNA <br />Protein<br />Tissue<br />Antibody<br />Cell & Cell Culture<br />Biomarker<br />Bioassay<br />Enzymes<br />Custom Service<br />Diagnostic Kit<br />13<br />
  17. 17. Nucleic Acid<br />Extraction and Purification<br />Gene Expression Profiling and Analysis<br />Gene Amplification and Cloning<br />Gene Regulation and Sequencing<br />Proteins<br />Extraction and Purification<br />Protein Expression Profiling and Analysis<br />Tissue Analysis & Antibodies<br />Tissue Section and Array<br />IHC and ISH<br />Conjugated Antibodies<br />Cell and Cell Culture<br />Primary Cell and Culture Media<br />Custom Service<br />Preclinical Animal Service<br />Cancer Patient Sample Collection and Analysis<br />Custom Antibodies<br />Molecular Biology Service<br />Product Application for Life Science Research<br />14<br />
  18. 18. Product Application forDiagnostics<br />Molecular Diagnostics<br /> PCR <br /> Sequencing<br />Immunodiagnostics<br /> ELISA<br /> Point of Care<br /> Strip - Colloidal Gold-Based Immunoassay<br />Biochemical Diagnostics<br /> Biochemical Detector and Bioassay<br />15<br />
  19. 19. Bio-Sample Preparation Process<br />Sample Collection<br />Storage & Transportation<br />Extraction & Purification<br />Extracts of Bio-Sample<br />Detection and Analysis<br />16<br />
  20. 20. Bio-Sample Collection <br />Sample Resource<br />From licensed venders and Hospitals with Supply Agreement<br />Collected under strict IRB ethical consenting practices (human patient samples)<br />Annotated with associated clinical data and pathological diagnosis<br />Preserved using stringent protocols to maximize sample quality<br />Species <br />Human: Normal, Cancer, Diseases<br />Animal: Monkey, Mouse, Nude Mouse, Rat, Rabbit, Hamster, Porcine, Guinea Pig, Bovine <br />Plant: Arabidopsis , Corn , Wheat , Soy bean , Potato , Rice , Orange <br />Type<br />Tissue, Whole Blood, Serum, Plasma, Urine, Stool<br />17<br />
  21. 21. Vast Tissue Variety <br />Broadest tissue collection <br />Brain<br />Throat <br />Thyroid<br />Lymphoma<br />Esophagus<br />Lung<br />Artery<br />Breast <br />Spleen<br />Liver <br />Bone marrow<br />Gallbladder<br />Stomach<br />Kidney <br />Vein<br />Pancreas<br />Skin <br />Intestine, colon, rectum<br />Bladder<br />Ovary, Uterus, Cervix, Testis<br />Prostate<br />
  22. 22. Total RNA, mRNA, microRNA <br />Quality <br />High quality RNA for high efficiency in PCR, reverse transcription, and microRNA studies <br />Extensive quality control procedures <br />MicroRNA certified total RNA<br />Documentation of donors' clinical history available<br />Variety<br />Human normal, cancer (primary and metastasis) and diseased tissues & blood<br />Matched Pairs: normal and primary pair (PP), primary and metastatic pair (PM)<br />Control genomic DNA, human and animal<br />Human cancer cell lines<br />Animals and plants<br />Format<br />Single organ, single donor or multi-donors, male or female<br />Multi-organs, single donor or multi-donors, male or female<br />19<br />
  23. 23. Total RNA and mRNA <br /> Normal: >50 Organs<br />Vast Variety <br /> Cancers: >40 Types<br /> Cancer Matched Pair: Normal & primary/primary and metastatic<br /> Neurological<br /> Diabetic<br /> Cancer cell lines: Hela, Jurkat, A431, K562, MCF7, Raji<br />Human<br /> Cardiovascular<br /> Adult diseased<br /> Pulmonary<br /> Liver cirrhosis<br /> Lupus disease<br />Total and message RNA<br /> Fetal normal: >30 Organs<br />Animal: Bovine, Equine, Chicken, Dog, Mouse, Rat, Hamster, <br /> Guinea pig, Rabbit, Cynomolgus monkey, Rhesus monkey<br />Plant<br />Human (male and female): multiple organs<br />Control Genomic DNA<br />Animal (male and female): Bovine, Equine, Chicken, Dog, Mouse, Rat, Hamster, Guinea pig, Rabbit, Cynomolgus monkey, Rhesus monkey<br />20<br />
  24. 24. Total RNA and mRNA<br />Features <br /><ul><li>Isolated from a wide variety of normal and diseased tissues
  25. 25. Applicable for microRNA regulation profiling
  26. 26. Documentation of tissues clinical history available</li></ul>Application<br /><ul><li>cDNA synthesis and cDNA library construction
  27. 27. Northern Blotting, RT-PCR, and RACE
  28. 28. MicroRNA study
  29. 29. cDNA probe for profiling study in gene expression
  30. 30. RNA protection and primer extension
  31. 31. RNA display
  32. 32. Purification of mRNA </li></ul>21<br />
  33. 33. Sample Collection Service Scope<br />Sample Collection<br /><ul><li>Body fluid and tumor samples according to various customer‘s</li></ul>specifications.  <br /><ul><li>Lung , Breast, Colon, Esophagus, Liver, Kidney, Stomach, Ovarian, Cervical, </li></ul>Uterus cancers. Other samples may be available upon request.<br />Sample Preparation<br /><ul><li>DNA/RNA/Protein Isolation
  34. 34. Tissue Sections and Tissue Arrays</li></ul>Sample Analysis<br /><ul><li>PCR Test for DNA and RNA
  35. 35. Western Analysis of Protein lysate
  36. 36. IHC and ISH test for tissue sections and tissue arrays</li></ul>22<br />
  37. 37. Clinical info collection<br />23<br />
  38. 38. Collection Network<br />Many hospitals in China <br />Tissue vendors world wide<br />BioChain<br />Customers<br />24<br />
  39. 39. Tissue arrays, sections, and panels<br />Quality <br /><ul><li>Ready-to-use for IHC, ISH, FISH, LCM, etc
  40. 40. Tissues selected by board-certified pathologists
  41. 41. Tissues collected under strict IRB and ethical consenting practices
  42. 42. Clinical data available including age, gender, tumor TNM and stage</li></ul>Variety<br />In both paraffin-embedded/FFPE and frozen formats<br />Human normal, cancer (primary and metastasis) and diseased tissues<br />Matched Pairs: normal and primary pair (PP), primary and metastatic pair (PM)<br />Human cancer cell lines<br />Animals and plants<br />25<br />
  43. 43. High-throughput tool for therapeutic and diagnostic antibody validation<br />30 human normal organs arrayed according to FDA guidelines<br />3 donors per organ for better statistics under FDA regulations<br />Cost- and time-efficient screening of cross-reactivity<br />FDA Standard FFPE Tissue Array available<br />FDA Standard Frozen Tissue Array<br />H &E stained FDA Standard Frozen Tissue Array. A total of 90 tissues spotted on 2 glass slides.<br />Examples of BioChain’s Frozen Tissue Array OCT Blocks<br />26<br />
  44. 44. Features <br />Large cancer tissue collection <br />Pre-arrayed and ready-to-use <br />12 to 150 different tissues are spotted on one array<br />In both paraffin-embedded/FFPE and frozen formats<br />Multi-cancers, Multi-donors, Multi-applications <br />Matched tissue DNA, RNA, protein and single sections available <br />Clinical Information<br />Tissue samples selected by board-certified pathologists, collected under strict IRB and ethical consenting practices<br />Accompanied with pathological and clinical annotation including patient age, gender, tumor diagnosis, tumor stage (or TNM), tumor size, tumor differentiation, tumor history and H&E images of the source, etc<br />Cancer Tissue Array<br />27<br />
  45. 45. Selected cancer tissue arrays<br />Cancer Tissue Array<br />For complete product list, visit or call 1-888-762-2568<br />28<br />
  46. 46. FFPE and Frozen Tissue Sections<br />Fig. 1 (left) In Situ Hybridization and Immunohistochemistry of p53 gene in human colon cancer tissue FFPE sections. Three serial paraffin embedded tissue sections were analyzed for p53 gene expression in human colon cancer tissue. It is shown that p53 mRNA and p53 protein are localized in the same area under 400x magnification.<br />Top: H.E. stained image<br />Middle: Image of immunohistochemistry<br />Bottom: Image of in situ hybridization<br />Fig. 2 (left) H. E. stained image of both paraffin embedded and frozen kidney tissue sections. <br />Top: Paraffin embedded kidney section<br />Bottom: Frozen kidney section <br />Tumor Matched Pair sections - Same Donor – Adjacent normal and Primary tumor pair - Primary and Metastasis tumor pair <br />A<br />B<br />C<br />D<br />Fig. 3 (above) H.E. stained tumor matched pair tissue sections.<br />A: Paraffin embedded breast cancer tissue<br />B: Paraffin embedded breast cancer adjacent normal tissue<br />C: Frozen breast cancer tissue<br />D: Frozen breast cancer adjacent normal tissue<br />29<br />
  47. 47. Sample Collection Service<br /><ul><li>Current ongoing project:</li></ul>1. Collect 400 FFPE lung cancer samples, and 200 matched serum samples<br />Perform FFPE tissue and circulating DNA isolation<br />Perform real time PCR with Roche L480<br />2. Collect pregnant women plasma from 100 donors and perform circulating DNA isolation<br />3. Collection 4 types of matched paired frozen tumor tissue, 25-30 donors from each type of tumor<br />Sample Collection<br />Sample Analysis<br />Sample Preparation<br />
  48. 48. IHC Service<br />CD 29 Negative Control<br />L0061<br />L0039<br />L0055<br />L0039 has stronger expression of CD 29. Due to our OCT blocks were made for micro dissection RNA quality, we have quick frozen procedure, which possibly generates ice crystal. However, the staining is clearly showed and negative control is listed as well. <br />31<br />
  49. 49. HMGA1 Is Induced by Wnt/β-Catenin Pathway and Maintains Cell Proliferation in Gastric Cancer Shin-ichi Akaboshi, et al. Am. J. Pathol., Oct 2009; 175: 1675 - 1685. <br />Up-regulation of C-Terminal Tensin-like Molecule Promotes the Tumorigenicity of Colon Cancer through β-Catenin Yi-Chun Liao, et al. Cancer Res., Jun 2009; 69: 4563 - 4566.<br />HMGA2 Maintains Oncogenic RAS-Induced Epithelial-Mesenchymal Transition in Human Pancreatic Cancer Cells Sugiko Watanabe, et al. Am. J. Pathol., Mar 2009; 174: 854 - 868. <br />MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activityLifeng Liu, et al. J. Biol. Chem., Feb 2009; 284: 5165 - 5174. <br />Genetic and Epigenetic SLC18A2 Silencing in Prostate Cancer Is an Independent Adverse Predictor of Biochemical Recurrence after Radical ProstatectomyKarina Dalsgaard Sørensen, et al. Clin. Cancer Res., Feb 2009; 15: 1400 - 1410. <br />References<br />
  50. 50. Summary<br /><ul><li>A leading provider of thousands of biological samples and integrated solutions.
  51. 51. 16 years of growing company experience
  52. 52. Highly trained scientists
  53. 53. Global business with the advantages of both US and China
  54. 54. Quality, cost, and reliability</li></li></ul><li>Conclusion<br />BioChain can provide reliable and cost-effective reagents for sequencing sample preparation<br />BioChain has solid experiences for OEM reagents for HTS equipment companies <br />High quality and excellent service<br />34<br />
  55. 55. Thank you!<br />35<br />