Fermentation is a metabolic process that converts sugar to acids, gases or alcohol. It occurs in yeast and bacteria, and also in oxygen-starved ( Deficient ) muscle cells, as in the case of lactic acid fermentation.
Fermentation, chemical process by which molecules such as glucose are broken down anaerobically. More
Fermentation is a metabolic process that converts sugar to acids, gases or alcohol. It occurs in yeast and bacteria, and also in oxygen-starved ( Deficient ) muscle cells, as in the case of lactic acid fermentation.
Fermentation, chemical process by which molecules such as glucose are broken down anaerobically. More
Fermentation in food processing is the process of converting carbohydrates to alcohol or organic acids using microorganisms—yeasts or bacteria under anaerobic conditions.
Or
Any metabolic process that releases energy from a sugar or other organic molecule, does not require oxygen or an electron transport system, and uses an organic molecule as the final electron acceptor
Fermentation usually implies that the action of microorganisms is desired.
The science of fermentation is known as zymology.
in microorganisms, fermentation is the primary means of producing ATP by the degradation of organic nutrients anaerobically
This presentation detail introduction to Fermentation.
This Presentation contains,
Introduction to Fermentation.
Media Formulation.
Historical Background.
Types of Fermentation.
Production of Antibiotics.
Production of Vitamins
Production of Statins.
Explain the difference between fermentation and respiration in biolo.pdfindiaartz
Explain the difference between fermentation and respiration in biology?
Solution
Fermentation and Respiration are the two widely used processes in biology.
Respiration : It is a biochemical process in which food molecules are broken down by cells of an
organism with the release of carbon dioxide , water and ATP ( energy currency of cells).
Food + oxygen gives carbondioxide + water + ATP.
Types : 1. Aerobic respiration : Occurs in presence of oxygen, performed by eukaryotic cells,
yields 38 ATP molecules and Occurs in both cytosol and mitochondria.
2 : Anaerobic Respiration : Occurs in absence of oxygen, performed by prokaryotic cells, yields
only 2 ATP molecules and Occurs in cytosol only.
Fermentation : Term ist used by Louis Pasteur in 19th century.
It is a metabolic process in which sugar is converted to acids or alcohol.
It occurs in yeast and bacteria and sometimes in muscle cells ( lactic acid fermentation )
Fermentation Occurs when electron transport chain becomes defunct due to lack of final electron
acceptor I,e oxygen.
Difference between Respiration and Fermentation:
1. Respiration uses oxygen as electron acceptor to form ATP while fermentation uses inorganic
donars ( Sulphur) to form ATP.
2. Both the processes convert nutrients from sugars to form ATP but they differ in their
processes and the amount of energy (ATP) produced.
3. Respiration produces 38 ATP molecules while fermentation produces only 2 ATP molecules..
Dive into the fascinating world of plant respiration with our comprehensive guide designed for Respiration in Plants Class 11 notes students. Learn how plants convert organic molecules into energy, discover the differences between aerobic and anaerobic respiration, and understand the crucial role of respiration in sustaining plant life and growth.
For more information, visit-www.vavaclasses.com
Heterotrophic Metabolism
Bacterial Metabolism heterotrophic metabolism is the biological oxidation of organic substances such as glucose to produce ATP and simpler organic (or inorganic) chemicals that the bacterial cell need for biosynthetic or assimilatory activities.
Respiration
Respiration is a kind of heterotrophic metabolism that utilizes oxygen and produces 380,000 calories from the oxidation of one mole of glucose. (Another 308,000 calories are wasted as heat.)
Heterotrophic Metabolism
Bacterial Metabolism heterotrophic metabolism is the biological oxidation of organic substances such as glucose to produce ATP and simpler organic (or inorganic) chemicals that the bacterial cell need for biosynthetic or assimilatory activities.
Respiration
Respiration is a kind of heterotrophic metabolism that utilises oxygen and produces 380,000 calories from the oxidation of one mole of glucose. (Another 308,000 calories are wasted as heat.)
Krebs Cycle
The Krebs cycle is the oxidative mechanism in respiration that fully decarboxylates pyruvate (through acetyl coenzyme A). 15 moles of ATP (150,000 calories) are produced by the route.
Glyoxylate Cycle
The glyoxylate cycle, seen in some bacteria, is a variant of the Krebs cycle. The oxidation of fatty acids or other lipid molecules produces acetyl coenzyme A.
Electron Transport and Oxidative Phosphorylation
ATP is produced in the last stage of respiration by a series of electron transfer processes within the cytoplasmic membrane that drive the oxidative phosphorylation of ADP to ATP. For this process, bacteria utilise a variety of flavins, cytochrome and non-heme iron components, as well as several cytochrome oxidases.
Fermentative metabolism and development of bioprocessing technology, processi...Ananya Sinha
This ppt includes, fermentation, the metabolism, the bioprocessor, it's technology, and the recombinant products. It connects all these topics together. The outline for plants and animals is nearly same
Fermentation in food processing is the process of converting carbohydrates to alcohol or organic acids using microorganisms—yeasts or bacteria under anaerobic conditions.
Or
Any metabolic process that releases energy from a sugar or other organic molecule, does not require oxygen or an electron transport system, and uses an organic molecule as the final electron acceptor
Fermentation usually implies that the action of microorganisms is desired.
The science of fermentation is known as zymology.
in microorganisms, fermentation is the primary means of producing ATP by the degradation of organic nutrients anaerobically
This presentation detail introduction to Fermentation.
This Presentation contains,
Introduction to Fermentation.
Media Formulation.
Historical Background.
Types of Fermentation.
Production of Antibiotics.
Production of Vitamins
Production of Statins.
Explain the difference between fermentation and respiration in biolo.pdfindiaartz
Explain the difference between fermentation and respiration in biology?
Solution
Fermentation and Respiration are the two widely used processes in biology.
Respiration : It is a biochemical process in which food molecules are broken down by cells of an
organism with the release of carbon dioxide , water and ATP ( energy currency of cells).
Food + oxygen gives carbondioxide + water + ATP.
Types : 1. Aerobic respiration : Occurs in presence of oxygen, performed by eukaryotic cells,
yields 38 ATP molecules and Occurs in both cytosol and mitochondria.
2 : Anaerobic Respiration : Occurs in absence of oxygen, performed by prokaryotic cells, yields
only 2 ATP molecules and Occurs in cytosol only.
Fermentation : Term ist used by Louis Pasteur in 19th century.
It is a metabolic process in which sugar is converted to acids or alcohol.
It occurs in yeast and bacteria and sometimes in muscle cells ( lactic acid fermentation )
Fermentation Occurs when electron transport chain becomes defunct due to lack of final electron
acceptor I,e oxygen.
Difference between Respiration and Fermentation:
1. Respiration uses oxygen as electron acceptor to form ATP while fermentation uses inorganic
donars ( Sulphur) to form ATP.
2. Both the processes convert nutrients from sugars to form ATP but they differ in their
processes and the amount of energy (ATP) produced.
3. Respiration produces 38 ATP molecules while fermentation produces only 2 ATP molecules..
Dive into the fascinating world of plant respiration with our comprehensive guide designed for Respiration in Plants Class 11 notes students. Learn how plants convert organic molecules into energy, discover the differences between aerobic and anaerobic respiration, and understand the crucial role of respiration in sustaining plant life and growth.
For more information, visit-www.vavaclasses.com
Heterotrophic Metabolism
Bacterial Metabolism heterotrophic metabolism is the biological oxidation of organic substances such as glucose to produce ATP and simpler organic (or inorganic) chemicals that the bacterial cell need for biosynthetic or assimilatory activities.
Respiration
Respiration is a kind of heterotrophic metabolism that utilizes oxygen and produces 380,000 calories from the oxidation of one mole of glucose. (Another 308,000 calories are wasted as heat.)
Heterotrophic Metabolism
Bacterial Metabolism heterotrophic metabolism is the biological oxidation of organic substances such as glucose to produce ATP and simpler organic (or inorganic) chemicals that the bacterial cell need for biosynthetic or assimilatory activities.
Respiration
Respiration is a kind of heterotrophic metabolism that utilises oxygen and produces 380,000 calories from the oxidation of one mole of glucose. (Another 308,000 calories are wasted as heat.)
Krebs Cycle
The Krebs cycle is the oxidative mechanism in respiration that fully decarboxylates pyruvate (through acetyl coenzyme A). 15 moles of ATP (150,000 calories) are produced by the route.
Glyoxylate Cycle
The glyoxylate cycle, seen in some bacteria, is a variant of the Krebs cycle. The oxidation of fatty acids or other lipid molecules produces acetyl coenzyme A.
Electron Transport and Oxidative Phosphorylation
ATP is produced in the last stage of respiration by a series of electron transfer processes within the cytoplasmic membrane that drive the oxidative phosphorylation of ADP to ATP. For this process, bacteria utilise a variety of flavins, cytochrome and non-heme iron components, as well as several cytochrome oxidases.
Fermentative metabolism and development of bioprocessing technology, processi...Ananya Sinha
This ppt includes, fermentation, the metabolism, the bioprocessor, it's technology, and the recombinant products. It connects all these topics together. The outline for plants and animals is nearly same
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
2. Objectives of Course
•Appreciate the use of microorganisms
technology.
• Genetic engineering applications in relation
in fermentation
to production of
pharmaceuticals.
Learning Outcomes
• Students will learn about various fermentation methods
including their sterlization methods, aeration and stirring methods.
•They will also learn about various types of fermentor design and
parameters used to control fermentation process.
3. Definition:
Fermentation is the chemical transformation of organic
substances into simpler compounds by the action of
enzymes, complex organic catalysts, which are produced by
microorganisms such as molds, yeasts, or bacteria.
Enzymes act by hydrolysis, a process of breaking down or
predigesting complex organic molecules to form smaller
(and in the case of foods, more easily digestible)
compounds and nutrients.
4.
5. HISTORY OF FERMENTATION
Fermentation is a natural process. People applied
fermentation to make products such as wine, mead (Made of
Fermented honey and water), cheese and beer long before the
biochemical process was understood. In the 1850s and
1860s Louis Pasteur became the first zymurgist (One who
6. expert in the field of fermentation) or scientist to study
fermentation when he demonstrated fermentation was
caused by living cells.
The first solid evidence of the living nature of yeast
appeared between 1837 and 1838 when three publications
appeared by C. Cagniard de la Tour, T. Swann, and F.
Kuetzing, each of whom independently concluded as a
result of microscopic investigations that yeast was a living
organism that reproduced by budding. The word "yeast," it
7. should be noted, traces its origins back to the Sanskrit word
meaning "boiling." It was perhaps because wine, beer, and
bread were each basic foods in Europe, that most of the early
studies on fermentation were done on yeasts, with which
they were made. Soon bacteria were also discovered; the
term was first used in English in the late 1840s, but it did not
come into general use until the 1870s, and then largely in
connection with the new germ theory of disease.
The view that fermentation was a process initiated by living
organisms soon aroused fierce criticism from the finest
8. chemists of the day, especially Justus von Liebig, J.J.
Berzelius, and Friedrich Woehler. This view seemed to give
new life to the waning mystical philosophy of vitalism, which
they had worked so hard to defeat. Proponents of vitalism held
that the functions of living organisms were due to a vital
principal (life force, chi, ki, prana , etc.) distinct from physico-
chemical forces, that the processes of life were not explicable
by the laws of physics and chemistry alone, and that life was in
some part self determining. As we shall soon see, the vitalists
played a key role in debate on the nature of
9. fermentation. A long battle ensued, and while it was
gradually recognized that yeast was a living organism, its
exact function in fermentations remained a matter of
controversy. The chemists still maintained that fermentation
was due to catalytic action or molecular vibrations.
The debate was finally brought to an end by the great French
chemist Louis Pasteur (1822-1895) who, during the 1850s
and 1860s, in a series of classic investigations, proved
conclusively that fermentation was initiated by living
organisms. In 1857 Pasteur showed that lactic acid
10. fermentation is caused by living organisms. In 1860 he
demonstrated that bacteria cause souring in milk, a process
formerly thought to be merely a chemical change, and his
work in identifying the role of microorganisms in food
spoilage led to the process of pasteurization. In 1877,
working to improve the French brewing industry, Pasteur
published his famous paper on fermentation, Etudes sur la
Biere , which was translated into English in 1879 as Studies
on Fermentation . He defined fermentation (incorrectly) as
"Life without air," but correctly showed specific types of
11. microorganisms cause specific types of fermentations and
specific end products. In 1877 the era of modern medical
bacteriology began when Koch (a German physician; 1843-
1910) and Pasteur showed that the anthrax bacillus caused
the infectious disease anthrax. This epic discovery led in
1880 to Pasteur's general germ theory of infectious disease,
which postulated for the first time that each such disease
was caused by a specific microorganism. Koch also made
the very significant discovery of a method for isolating
microorganisms in pure culture.
12. TYPES BASED ON RESPIRATION (AEROBIC AND
AN-AEROBIC)
Aerobic Fermentation: Aerobic fermentation means that
oxygen is present. Wine, beer and acetic acid vinegar (such
as apple cider vinegar), need oxygen in the “primary” or
first stage of fermentation.
When creating acetic vinegar, for example, exposing the
surface of the vinegar to as much oxygen as possible,
creates a healthy, flavorful vinegar with the correct pH.
13. Anaerobic Fermentation: Anaerobic fermentation is a
method cells use to extract energy from carbohydrates
when oxygen or other electron acceptors are not available
in the surrounding environment. This differentiates it from
anaerobic respiration, which doesn’t use oxygen but does
use electron-accepting molecules that come from outside
of the cell. The process can follow glycolysis as the next
step in the breakdown of glucose and other sugars to
produce molecules of adenosine triphosphate (ATP) that
create an energy source for the cell.
14. Through this method, a cell is able to regenerate
nicotinamide adenine dinucleotide (NAD+) from the
reduced form of nicotinamide adenine dinucleotide
(NADH), a molecule necessary to continue glycolysis.
Anaerobic fermentation relies on enzymes to add a
phosphate group to an individual adenosine diphosphate
(ADP) molecule to produce A
TP
, which means it is a form
of substrate-level phosphorylation. This contrasts with
oxidative phosphorylation, which uses energy from an
established proton gradient to produceA
TP
.
15. There are two major types of anaerobic fermentation:
ethanol fermentation and lactic acid fermentation. Both
restore NAD+ to allow a cell to continue generating ATP
through glycolysis.
Ethanol fermentation: Ethanol fermentation converts two
pyruvate molecules, the products of glycolysis, to two
molecules of ethanol and two molecules of carbon dioxide.
The reaction is a two-step process in which pyruvate is
converted to acetaldehyde and carbon dioxide first, by the
enzyme pyruvate decarboxylase.
16. Yeast and certain bacteria perform ethanol
where pyruvate (from glucose
fermentation
metabolism) is broken into ethanol and
carbon dioxide. The net chemical
for the production of ethanol from
equation
glucose
is:
C6H12O6 (glucose) → 2 C2H5OH (ethanol) + 2 CO2 (carbon dioxide)
Ethanol fermentation is used the production of beer, wine
and bread. It's worth noting that fermentation in the presence
of high levels of pectin result in the production of small
amounts of methanol, which is toxic when consumed.
18. Lactic acid fermentation: Lactic acid fermentation is a
biological process by which glucose and other six-carbon
sugars (also, disaccharides of six-carbon sugars, e.g.
sucrose or lactose) are converted into cellular energy and
the metabolite lactate.
The pyruvate molecules from glucose metabolism
(glycolysis) may be fermented into lactic acid. Lactic acid
fermentation is used to convert lactose into lactic acid in
yogurt production. It also occurs in animal muscles when
19. the tissue requires energy at a faster rate than oxygen can be
supplied. The next equation for lactic acid production from
glucose is:
C6H12O6 (glucose) → 2 CH3CHOHCOOH (lacticacid)
The production of lactic acid from lactose and water may be
summarized as:
C12H22O11 (lactose) + H2O (water) → 4 CH3CHOHCOOH
(lactic acid)
Yogurt is made by fermenting milk. It's high in protein,
calcium, and probiotics ("good" bacteria). Here's how to
make yogurt and a look at the chemistry of yogurt.
20. TYPES OFFERMENTATION
Homo Lactic fermentation: The fermentation in which only
the lactic acid is produced. There is no any side product
formed after the reaction.
Hetero-Lactic Fermentation: The Fermentation in which the
lactic acid is produced along with some by products like
gases.
21. MECHANISM OF FERMENTATION
Fermentation takes place when the electron transport chain is
unusable (often due to lack of a final electron receptor, such
as oxygen), and becomes the cell’s primary means of ATP
(energy) production.[1] It turns NADH and pyruvate
produced in glycolysis into NAD+ and an organic molecule
(which varies depending on the type of fermentation; see
examples below). In the presence of O2, NADH and pyruvate
are used to generateA
TPin respiration. This iscalled
22. oxidative phosphorylation, and it generates much more ATP than
glycolysis alone. For that reason, cells generally benefit from
avoiding fermentation when oxygen is available, the exception
being obligate anaerobes which cannot tolerate oxygen.
The first step, glycolysis, is common to all fermentation
pathways this is the cause of fermentation:
C6H12O6 + 2 NAD+ + 2 ADP + 2 Pi → 2 CH3COCOO− + 2
NADH + 2A
TP+ 2 H2O + 2H+
Pyruvate is CH3COCOO−. Pi is inorganic phosphate. Two ADP
molecules and two Pi are converted to two ATP and two water
molecules via substrate-level phosphorylation. Two molecules of
NAD+ are also reduced to NADH.
23. In oxidative phosphorylation the energy for A
TP formation
is derived from an electrochemical proton gradient
generated across the inner mitochondrial membrane (or, in
the case of bacteria, the plasma membrane) via the
electron transport chain. Glycolysis has substrate-level
phosphorylation (ATP generated directly at the point of
reaction).
Humans have used fermentation to produce food and
beverages since the Neolithic age. For example,
24. fermentation is used for preservation in a process that
produces lactic acid as found in such sour foods as
pickled cucumbers, kimchi and yogurt (see fermentation
in food processing), as well as for producing alcoholic
beverages such as wine (see fermentation in
winemaking) and beer. Fermentation can even occur
within the stomachs of animals, such as humans.
32. FERMENTATION AS A FOOD PRESERVATION
TECHNIQUE
Fermented foods are foods that have been prepared in a way so
that the bacteria naturally found within them starts to ferment.
Fermentation, also known as lacto-fermentation, is a chemical
process in which bacteria and other micro-organisms break
down starches and sugars within the foods, possibly making
them easier to digest, and resulting in a product that is filled
with helpful organisms and enzymes.
This process of fermentation is a natural preservative, which
means that fermented foods can last a long time.
33. ADVANTAGES AND DIS ADVANTAGES OF
FERMENTATION
Advantages of fermentation are that lactic acid can be
produced and it can produce energy forATPs.
Disadvantages of fermentation are that production can be
slow, the product is impure and needs to have further treatment
and the production carries a high cost and more energy.
34. IMPORTANCE OF FERMENTATION
Fermentation is important to cells that
don't have oxygen or cells that don't use
oxygen because:
1.It allows the cells to get 2 ATPgain from onemolecule
of glucose, even without oxygen.
2.Fermentation takes away the end products of glycolysis
so glycolysis can continue ... freeing up the electron
carriers, and so on.
35. 3.Fermentation is important to the baking industry because
it is the process that yeast uses to produce the bubbles of
carbon dioxide that make the dough rise.
4.Fermentation is important in wineries and breweries
because yeast uses fermentation to produce alcohol.
5.Fermentation is important in muscles because it allows the
muscles to keep getting a little energy from glucose even
when the oxygen supply can't keep up with the demand.
37. Introduction
Blood: It consistsof
1. Cells i.e. RBC, WBC, Platelets
2. Plasma i.e. Proteins, Coagulation factors
Blood Substitutes: It consistsof
1. Volume Expanders
2. Synthetic oxygen carriers
Blood Products: : Any therapeutic substance prepared from the blood. It consists of
Blood Components
Constituent separated from whole
blood
Plasma Derivatives
1. Red cell concentrate
2. Leuko-reduced RBC
3. Plasma
4. Plasma derivatives
5. Granulocyte concentrate
6. Platelet concentrate
1. Fresh frozen plasma
2. Cryoprecipitate
3. Albumin
4. Coagulation factors concentrate
5. Immunoglobulins
38. Collection
350ml{301ml blood+49ml anticoagulant} is taken from previously
screened person .
Tested for- syphilis, HBsAg, HCV, HIV 1&2
Platelet concentrates for bacterial contamination
Group determination (ABO & Rh) & presence of any RBC
antibody
Processed into sub-components
39. Criteria for donor
▶ Normal body temperature, Blood pressure
▶ Weight above 50-55 kgs
▶ Normal Hb levels..
▶ Free from RTI, skin dz or blood dz
▶ No h/o drug addiction
▶ No h/o viral hepatitis
▶ No HIV infection or risk for it
▶ No h/o blood transfusion (<=6mo)
40. Whole Blood
▶ It is donor blood mixed with an anticoagulant
▶ Collected by venesection
▶ Collected in 63 ml of anticoagulant to form 450 ml.
“ “ “ 350 ml
OR, in 49 ml “ “ “
▶ Stored at 1-6º C
▶ Shelf Life up to 5 weeks
▶ 1 unit{350ml} will increase the Hb level of an
Adult (60-70Kg)
Pediatric pts
by 0.8 gm/dl
by 1gm/dl
41. • Indication :
• Exchange transfusions.
• Hemorrhage (>= 20%blood loss):
-To ↑O₂ carrying capacity
-volume replacement
-stabilize coagulation factors
Advantages :
• simple and inexpensive
• no special equipment required for
processing
Disadvantages :
•Risk of circulatory overload
•Maintaining at ~4oC leads to:
-platelet dysfunction
-degradation of coagulation
factors
-decreased 2,3 DPG levels
• Febrile reactions
• Narrow Shelf life- 5wks
42. Preservation techniques
▶ Chemical incorporation
▶ Rejuvenation solutions
▶ Additive solutions
▶ Red cell freezing
▶ Addition of buffers
43. Anticoagulants
Citrate -chief component of almost all anticoagulants used
chelates Ca2+ ... stops coagulation
Dextrose- energy source.
Phosphate- buffer
1. CG (sod.citrate+glucose)- 1st soln ever used
2. ACD (acid+citrate+dextrose)
3. CPD (citrate+phosphate+dextrose)- Higher pH & 2,3 DPG level is maintained.
SHELF-LIFE is 21 days.
44. 4.CPDA
(+adenine)-
ATP production
ADP levels- glycolysis-
increases RBC viability to 35 days
5.SAG-M (saline+adenine+glucose+mannitol)-
maintains cell nutrition
increases viability to 42 days,
M-prevents spontaneous hemolysis
45. Red cel concentrate
Blood to separate under gravity (sedimentation method)
Or,Centrifugation done in special refrigerated centrifuge
Most of the plasma is removed and replaced with a solution of
glucose and adenine in saline to maintain viability of red cells.
Maintained at temp : 2ºC to 6ºC
High O₂ carrying capacity
Optimal target for infusion- 7g/dl
Indications:
- replacement of red cells in anemic patients
- acute massive blood loss
46. Advantages :
Easy to prepare
To avoid volume overload in c/o CCF
Less chances of infection or alloimmunization
Less immunosuppressant
Dec. allergic reaction if plasma is also removed
Disadvantages :
High Red cells to plasma ratio ↑viscosity
.·. ↑ time required for passing
through cannula & vessels
thus Hct should not exceed 80%
47. Platelet concentrates
Platelets separated from plasma obtained after 4-6
donations are pooled
or,
from a single donor by plateletapheresis
Composed of mainly platelets, some nonfunctional
WBCs, few RBCs & plasma[maintains pH].
volume= 50 ml contains 5.5x10^9/lt plts
Stored at 20-24oC
Shelf life- 5 days.. Once opened, transfuse within 6 hrs
1 unit of PC increases platelet count by: 5000-10000(adults)
20,000 (children)
75,000-1,00,000(infants)
48. If single donor- 1 unit of PC should contain >5.5 х 10 9platelets
If pooled then, > 250х 109 platelets
• Indications:
- Thrombocytopenia
- Platelet dysfunction
- Complication of anti-platelet
therapy(Clopidogrel)
- DIC
49. Fresh Frozen Plasma
Stored at -40 to -50oC
Composed of plasma, all coagulation factors, albumin & Ig
1 unit= 200-250ml
Plasma removed from a unit of whole blood & frozen (by
immersing in a solid carbondioxide and ethyl alcohol
mixture) at/ below -25ºC within 4 hrs. ofcollection.
Each unit of FFP increases the level of each clotting
factor by 2-3 %
Shelf life: Frozen—1 year(<-30 degree centigrade)
50. Indications:
-active bleeding in pts with multiple factor
deficiencies
-surgery in patient with liver failure(treatment of choice)
-after massive transfusion
-DIC
-rapid reversal of warfarin(Prothrombim complex
concentrates,with factors II,IX,X can also be given)
-TTP
Advantage:
it is a acellular component so no chance of transmission of
intracellular infection
51. Cryoprecipitate
▶ When FFP is allowed to Thaw at 4oC, glutinous precipitate remains and,
if the suprenatant plasma is removed, cryoprecipitate
▶ contains factors VIII (very rich) , fibrinogen as well as factor XIII &
vwf.
at -40oC
▶Shelf life:2 years , if frozen
Used
1. If fibrinogen <1g/dl due to dilution
2. DIC
3. Von Willebrand disease and hemophilia VIII deficiency
52. Coagulation Factors
Factor VIII concentrate
Prepared from large pools of donor plasma by fractionation process.
Commercially prepared, lyophilized powder
Hemophilia A & von Willebrand’s disease.
Storage : 2 to 6°C
Factor IX
Commercially prepared, lyophilized powder
Hemophilia B
Contains Factor II (prothrombin), VII,IX,X.
Purified Factor IX contains only IX.
Refrigerated at 35-45 °F
53. Immunoglobulin
▶ It is a concentrated solution of IgG
antibody component of plasma
▶ Prepared from large pools of donors
▶ Uses
1. To reduce infective complications in
patients with Ab deficiencies
2. Immunological d/o- Immune
thrombocytopenia, GBS
3. Anti-zoster Ig in varicella zoster
prophylaxis
4. Anti-Rhesus D Ig in pregnancy to
prevent hemolytic dsz in newborn
▶ Can Cause
▶ Acute renal failure ( in elderly)
▶ Acute reactions
Fibrinogen
▶ Prepared by organic liquid fractionation of plasma
▶ Stored in dried form
▶ Can be reconstituted with distilled water
▶ Used in cases of severe fibrinogendepletion
eg DIC, congenital afibrinogenaemia
▶ Carry high risk of hepatitis
55. Crystalloids
a) Is isotonic, but with the
metabolism of glucose
inside the body, becomes
hypotonic.
b) Blood cannot be given through
the same drip set otherwise
rouleaux formation will cause
clumping of RBCs
4) Dextrose Normal Saline (DNS)
a) Is hypertonic
b) But 1/5 NS +4.3% dextose and ¼ NS +5%
dextrose are isotonic and are best used as
maintenance fluids.
1) ) Ringer’s Lactate Solution(Hartman
Solution)
a) Consists of Na, Cl, K, Ca, Lactate. pH=6.5,
Osmolarity=273mmol/lt (slightly hypotonic)
b) Blood should not be given through the same drip set as
it contains Ca.
c) Crystalloid of choice for blood loss replacement.
2) ) Normal Saline {0.9% NaCl (isotonic)}
a) Preferred over RL for treating
• Hypochloremic metabolic alkalosis
• Brain injury (Ca2+ and lactate can increase the
neuronal injury)
• Hyponatremia
▶ 3)5% Dextose
56. Colloids
▶
▶ 1) Dextrans (Lomodex)
▶ Polysaccharides, can be stored for 10 years, half life=2-8 hours
▶ Advantages
a) Non toxic, neutral and chemically inert
b) Low molecular wt dextran improves microcirculation
▶ Drawbacks
a) Interfere with blood grouping and cross matching (by causing RBC aggregation, so a
blood sample should be taken before-hand)
b) Interferes with platelet function and is a/w abnormal bleeding (so total volume given
should not exceed 1000ml)
c) Can cause severe anaphylaxis
d) Large molecular weight dextrans can block renal tubules
e) ARDS (rarely) because of direct toxic effect on pulmonary capillaries
57. ▶ 2)Albumin(available as 5% and 20% solution)
▶ Very expensive, intravascular half-life= 10-15days
▶ Used in protein loss like, peritonitis, liver failure, burns, protein losing enterepathies.
▶ 20% is hypertonic and expands plasma volume by more than the amount infused.
▶ Stored for several months in liquid form at 4degree
▶ 3)Gelatins (Haemaccel)(available as 3.5% solution)
▶ Consists of Gelatin, Na, Cl, K, Ca
▶ Expand plasma effectively for 2 hours
▶ At clinically used doses, these do not interfere with blood grouping, plt fxn and clotting
but at high doses can interfere clotting.
▶ As it contains high Calcium, citrated blood should not be mixed.
58. Synthetic oxygen carriers:
mimic blood's O₂ transport ability
Types
1. Abiotic - perfluorocarbons, perfluoroctyl bromide
2. Biomimetic - HBOC( Hb based oxygen carriers)
- recombinant erythropoietin, hemoglobin under clinical trials
some available products:
Hemopure
Oxygent
PolyHeme
59. Perfluorocarbons
▶ High O₂ carryingcapacity
▶ Emulsified and transfused
▶ Short survival in the circulation
▶ Adverse effects : Flu like symptoms.
Immunologic effects.
HBOC
▶ Hb synthesised by controlled lysis of Red
Cells
▶ Short half life as compared to RBCs
▶ Side effects :
GI distress, neurotoxicity,
vasoconstriction,
interfere with macrophage system.