This slide is basically a conclusive result of various Research journals based on different disappearing ink's Examination and Analysis.
The presentation comprises of research papers from Journal of Forensic science mainly from edition 1st - 8th.
Hope this slide will be helpful for you all. Please have a look
Gracias !
age of document , document, paper, writing matter, typescript, printed matter, signature and handwriting, typewriter and various types of type writing devices
Alteration is defined as any change to a document which gives it a different effect from
that it originally possessed. If change is made after execution of a document and without
the consent of the other party or concerned person, then such change is called fraudulent
alteration. Document examiners must be able to identify documents that were altered to change the conditions of a contract or other legal entity.
Alterations can take the form of erasures and replacement and/or insertion of material into a document.
age of document , document, paper, writing matter, typescript, printed matter, signature and handwriting, typewriter and various types of type writing devices
Alteration is defined as any change to a document which gives it a different effect from
that it originally possessed. If change is made after execution of a document and without
the consent of the other party or concerned person, then such change is called fraudulent
alteration. Document examiners must be able to identify documents that were altered to change the conditions of a contract or other legal entity.
Alterations can take the form of erasures and replacement and/or insertion of material into a document.
forensic questioned document examinationkiran malik
Power point presentation basically related to document examination in forensic science laboratories, related various facilities and was related to expert evidences.
VSC VIDEO SPECTRAL COMPARATAOR FORENSIC APPLICATIONS BY SHAILESH CHAUBEY STUDENT OF FORENSIC SCIENCE & CRIMINOLOGY FROM BUNDELKHAND UNIVERSITY JHANSI UTTAR PRADESH INDIA . THIS PPT SHOWS ABOUT THE FEATURES, APPLICATIONS , CASE LAWS & NEED OF VSC IN FORENSIC ASPECTS FOR DOCUMENT EXAMINATION & HANDWRITING . THIS PRESENTATION WILL HELP TO GET MORE INFORMATION ABOUT VSC BY VARIOUS SLIDES.
Encryption is one of the most important parts of modern application security. But the practice of obfuscating messages go far further back in history than many realise. In this talk we'll take a look at the development ; the differences between steganography and cryptography, between codes and ciphers, and the unceasing battle between cryptographers and cryptanalysts throughout history and the very human stories behind it.
Fingerprint - Everything You Need To Know About FingerprintsSwaroopSonone
A detailed fingerprint presentation. Fingerprint is one of the most important criminal investigation tools due to their two significant features- uniqueness and persistence. The unique features of friction ridge skin persist from before birth, i.e. during fetal development to the decomposition after death...
A digital imaging instrument manufactured by Foster + Freeman that employs combinations of light sources and filters to examine document evidence under various wavelengths of radiation ranging from ultraviolet to the infrared regions of the electromagnetic spectrum.
VSC is a preferred tool as it supports non destructive examination of documents. VSC uses multiple parameters like IR, UV, and White light providing accurate results.
Developing Latent Fingerprints on Wet Surfaces with a Fluorescent Schiffs Bas...ijtsrd
The fingerprints left by the perpetrators are the first thing a forensic team searches for during a crime investigation. These prints could be visible or hidden also known as latent . The development of these latent fingerprints on various surfaces is done using a variety of approaches. An important and useful method, most commonly used in forensic investigations for wet surfaces involves Small Particle Reagent method. In present work, we report the use of a UV fluorescent Schiffs base as an effective organic compound which has been employed as a small particle reagent. It has been used for the visualisation of latent finger prints on various non absorbent surfaces. The advantage of employing this Schiffs base as a small particle reagent over previously utilised dyes is that it is less expensive, contains no heavy metal, and may be used on a variety of surfaces. Furthermore, while taking images, visualisation does not necessitate the use of a particular UV lamp or filter. Krishan Kumar Arora | Sarita Passey | Ruby Mishra "Developing Latent Fingerprints on Wet Surfaces with a Fluorescent Schiff's Base as SPR" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46390.pdf Paper URL : https://www.ijtsrd.com/chemistry/other/46390/developing-latent-fingerprints-on-wet-surfaces-with-a-fluorescent-schiffs-base-as-spr/krishan-kumar-arora
Use of a Fluorescent Schiff’S Base as Developing Agent for Latent Finger Printsijtsrd
During investigations of a crime, the first thing that a forensic team looks for is the finger prints left by the culprits. These finger prints may be visible or latent. Various methods are used for the development of these latent fingerprints on different surfaces. Amongst these methods, powder method is still the most preferred one in forensic investigations due to its simplicity and efficiency. In this paper we report the application of a UV fluorescent schiff’s base as an efficient organic compound in various powder formulations for the visualization of latent finger prints on different absorbent and non absorbent surfaces. The results obtained showed clear ridge details in almost all the cases. The benefit of using this schiff’s base in powder formulations over the earlier used powders lies in the fact that it is economical, does not contain any heavy metal and can be used on objects of various colours. Further the visualisation does not require any specific UV lamp or filter to take the photographs. Krishan Kumar Arora | Sarita Passey "Use of a Fluorescent Schiff’S Base as Developing Agent for Latent Finger Prints" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-1 , December 2020, URL: https://www.ijtsrd.com/papers/ijtsrd38031.pdf Paper URL : https://www.ijtsrd.com/chemistry/other/38031/use-of-a-fluorescent-schiff’s-base-as-developing-agent-for-latent-finger-prints/krishan-kumar-arora
forensic questioned document examinationkiran malik
Power point presentation basically related to document examination in forensic science laboratories, related various facilities and was related to expert evidences.
VSC VIDEO SPECTRAL COMPARATAOR FORENSIC APPLICATIONS BY SHAILESH CHAUBEY STUDENT OF FORENSIC SCIENCE & CRIMINOLOGY FROM BUNDELKHAND UNIVERSITY JHANSI UTTAR PRADESH INDIA . THIS PPT SHOWS ABOUT THE FEATURES, APPLICATIONS , CASE LAWS & NEED OF VSC IN FORENSIC ASPECTS FOR DOCUMENT EXAMINATION & HANDWRITING . THIS PRESENTATION WILL HELP TO GET MORE INFORMATION ABOUT VSC BY VARIOUS SLIDES.
Encryption is one of the most important parts of modern application security. But the practice of obfuscating messages go far further back in history than many realise. In this talk we'll take a look at the development ; the differences between steganography and cryptography, between codes and ciphers, and the unceasing battle between cryptographers and cryptanalysts throughout history and the very human stories behind it.
Fingerprint - Everything You Need To Know About FingerprintsSwaroopSonone
A detailed fingerprint presentation. Fingerprint is one of the most important criminal investigation tools due to their two significant features- uniqueness and persistence. The unique features of friction ridge skin persist from before birth, i.e. during fetal development to the decomposition after death...
A digital imaging instrument manufactured by Foster + Freeman that employs combinations of light sources and filters to examine document evidence under various wavelengths of radiation ranging from ultraviolet to the infrared regions of the electromagnetic spectrum.
VSC is a preferred tool as it supports non destructive examination of documents. VSC uses multiple parameters like IR, UV, and White light providing accurate results.
Developing Latent Fingerprints on Wet Surfaces with a Fluorescent Schiffs Bas...ijtsrd
The fingerprints left by the perpetrators are the first thing a forensic team searches for during a crime investigation. These prints could be visible or hidden also known as latent . The development of these latent fingerprints on various surfaces is done using a variety of approaches. An important and useful method, most commonly used in forensic investigations for wet surfaces involves Small Particle Reagent method. In present work, we report the use of a UV fluorescent Schiffs base as an effective organic compound which has been employed as a small particle reagent. It has been used for the visualisation of latent finger prints on various non absorbent surfaces. The advantage of employing this Schiffs base as a small particle reagent over previously utilised dyes is that it is less expensive, contains no heavy metal, and may be used on a variety of surfaces. Furthermore, while taking images, visualisation does not necessitate the use of a particular UV lamp or filter. Krishan Kumar Arora | Sarita Passey | Ruby Mishra "Developing Latent Fingerprints on Wet Surfaces with a Fluorescent Schiff's Base as SPR" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46390.pdf Paper URL : https://www.ijtsrd.com/chemistry/other/46390/developing-latent-fingerprints-on-wet-surfaces-with-a-fluorescent-schiffs-base-as-spr/krishan-kumar-arora
Use of a Fluorescent Schiff’S Base as Developing Agent for Latent Finger Printsijtsrd
During investigations of a crime, the first thing that a forensic team looks for is the finger prints left by the culprits. These finger prints may be visible or latent. Various methods are used for the development of these latent fingerprints on different surfaces. Amongst these methods, powder method is still the most preferred one in forensic investigations due to its simplicity and efficiency. In this paper we report the application of a UV fluorescent schiff’s base as an efficient organic compound in various powder formulations for the visualization of latent finger prints on different absorbent and non absorbent surfaces. The results obtained showed clear ridge details in almost all the cases. The benefit of using this schiff’s base in powder formulations over the earlier used powders lies in the fact that it is economical, does not contain any heavy metal and can be used on objects of various colours. Further the visualisation does not require any specific UV lamp or filter to take the photographs. Krishan Kumar Arora | Sarita Passey "Use of a Fluorescent Schiff’S Base as Developing Agent for Latent Finger Prints" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-1 , December 2020, URL: https://www.ijtsrd.com/papers/ijtsrd38031.pdf Paper URL : https://www.ijtsrd.com/chemistry/other/38031/use-of-a-fluorescent-schiff’s-base-as-developing-agent-for-latent-finger-prints/krishan-kumar-arora
Antimicrobial Effect on Different Dyes and Inksijtsrd
The present study has been undertaken to find out the antimicrobial activity of methanol base ink. The antimicrobial activity was screened against ecoli, pseudomonas and staphylococcus. The dilutions of inks were prepared with different concentration, tested with respect to the bacterial strains and their inhibition was calculated. The methanolic extract of the ink of CAMLIN ink especially white board marker ink and parker gel pen ink with its blue and black colour respectively were prepared and the antimicrobial activity was determined by agar well diffusion method on different bacterial strains. It is been observed that with 10 dilution of samples Pseudomonas shows the maximum degradation of ink, in 20 dilution of samples staphylococcus and pseudomonas both shows the approximate equal degradation of ink, in 30 dilution of samples Pseudomonas shows maximum degradation, in 40 dilution of samples almost all bacteria shows the equally degradation in their mean positions, and in 50 dilution of sample only staphylococcus shows the degradation. Hence, it is very important to use the inks which show null or less sensitivity towards the microbes during the preparation of any important documents. Prasansha Singla | Shipra Rohatgi | Tripti Bhatnagar "Antimicrobial Effect on Different Dyes and Inks" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-4 , June 2020, URL: https://www.ijtsrd.com/papers/ijtsrd31634.pdf Paper Url :https://www.ijtsrd.com/chemistry/other/31634/antimicrobial-effect-on-different-dyes-and-inks/prasansha-singla
Comparative Analysis of Inkjet Printer Inks Extracted from Printed Documents ...IOSR Journals
Primary coloured inks i.e. Cyan, Magenta, Yellow, and Black used in inkjet printers were extracted from the printed document and analyzed in order to identify the possible functional groups using Infrared (IR) spectroscopic technique. Experiments were conducted on the all four primary inks and the results are presented. The technique can provide valuable information if an admitted sample is provided for comparison with the suspect printed document.
Schiff’s Bases as Potential Mapping Agents for Iron Traces on Skinijtsrd
Invisible impression of weapon which are left on holder’s palm form an important evidence during investigation of a crime. Identifying the traces of such metals is finding an increasing role of chemists in forensic science. A few reagents like 8 hydroxyquinoline and triazines have been tried for this purpose but their usage entails a number of drawbacks as well. We report here the utility of schiff’s bases as potential reagents for mapping the traces of iron left on skin after the suspect held the weapon made up of iron or an alloy of iron. Krishan Kumar Arora | Sarita Passey "Schiff’s Bases as Potential Mapping Agents for Iron Traces on Skin" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-6 , October 2020, URL: https://www.ijtsrd.com/papers/ijtsrd35710.pdf Paper Url: https://www.ijtsrd.com/chemistry/other/35710/schiff’s-bases-as-potential-mapping-agents-for-iron-traces-on-skin/krishan-kumar-arora
Similar to Examination of Disappearing Ink Writting (9)
Confirmatory Test for Semen identificationParth Chuahan
Seminal fluid is a complex mixture of secretions from at least four male urogenital glands. The seminal vesicle gland contributes approximately 60% to this mixture, the prostate gland contributes approximately 30%, and the combined contribution of the epididymis and bulbourethral glands account for the remaining 10%
Confirmatory Tests for semen:
1- The Christmas Tree Stain: The most reliable confirmation for the presence of semen is the positive visual identification of sperm cells (or spermatozoa) using the Christmas tree stain.
Measure of Dispersion, Range, Mean and Standard Deviation, Correlation and Re...Parth Chuahan
It is simply the difference between the maximum value and the minimum value given in a data set. Example: 1, 3,5, 6, 7 => Range = 7 -1= 6
Standard Deviation: The square root of the variance is known as the standard deviation i.e. S.D. = √σ
Mean and Mean Deviation: The average of numbers is known as the mean and the arithmetic mean of the absolute deviations of the observations from a measure of central tendency is known as the mean deviation (also called mean absolute deviation).
There are two main types of dispersion methods in statistics which are:
Absolute Measure of Dispersion
Relative Measure of Dispersion
The most commonly used techniques for investigating the relationship between two quantitative variables are correlation and linear regression. Correlation quantifies the strength of the linear relationship between a pair of variables, whereas regression expresses the relationship in the form of an equation.
Compound Microscope and Its Forensic Applications Parth Chuahan
The term “compound” in compound microscopes refers to the microscope having more than one lens.
Devised with a system of combination of lenses, a compound microscope consists of two optical parts, namely the objective lens and the ocular lens.
A compound microscope is of great use in pathology labs so as to identify diseases.
Various crime cases are detected and solved by drawing out human cells and examining them under the microscope in forensic laboratories.
The presence or absence of minerals and the presence of metals can be identified using compound microscopes.
Students in schools and colleges are benefited by the use of a microscope for conducting their academic experiments.
It helps to see and understand the microbial world of bacteria and viruses, which is otherwise invisible to the naked eye.
Plant cells are examined and the microorganisms thriving on it can be ascertained with the help of a compound microscope. Thereby, a compound microscope has proved to be crucial to biologists.
significance of fingerprinting , skin pattern and its collection with CASE ST...Parth Chuahan
Fingerprints are the tiny ridges, whorls and valley patterns on the tip of each finger. They form from pressure on a baby's tiny, developing fingers in the womb. ... Fingerprints are even more unique than DNA, the genetic material in each of our cells.
Fingerprints can be found on practically any solid surface, including the human body. Analysts classify fingerprints into three categories according to the type of surface on which they are found and whether they are visible or not: Fingerprints on soft surfaces (such as soap, wax, wet paint, fresh caulk, etc.) are likely to be three-dimensional plastic prints; those on hard surfaces are either patent (visible) or latent (invisible) prints. Visible prints are formed when blood, dirt, ink, paint, etc., is transferred from a finger or thumb to a surface. Patent prints can be found on a wide variety of surfaces: smooth or rough, porous (such as paper, cloth or wood) or nonporous (such as metal, glass or plastic).
Latent prints are formed when the body’s natural oils and sweat on the skin are deposited onto another surface. Latent prints can be found on a variety of surfaces; however, they are not readily visible and detection often requires the use of fingerprint powders, chemical reagents or alternate light sources. Generally speaking, the smoother and less porous a surface is, the greater the potential that any latent prints present can be found and developed.
Pairwise kinship analysis - By the Index Of Chromosome Sharing Using Single N...Parth Chuahan
a new approach for pairwise kinship analysis in forensic genetics based on chromosomal sharing between two individuals. Here, we defined “index of chromosome sharing” (ICS) calculated using 174,254 single nucleotide polymorphism (SNP) loci typed by SNP microarray and genetic length of the shared segments from the genotypes of two individuals. To investigate the expected ICS distributions from first- to fifth-degree relatives and unrelated pairs, we used computationally generated genotypes to consider the effect of linkage disequilibrium and recombination. The distributions were used for probabilistic evaluation of the pairwise kinship analysis, such as likelihood ratio (LR) or posterior probability, without allele frequencies and haplotype frequencies. Using our method, all actual sample pairs from volunteers showed significantly high LR values (i.e., ≥ 108); therefore, we can distinguish distant relationships (up to the fifth-degree) from unrelated pairs based on LR. Moreover, we can determine accurate degrees of kinship in up to third-degree relationships with a probability of > 80% using the criterion of posterior probability ≥ 0.90, even if the kinship of the pair is totally unpredictable. This approach greatly improves pairwise kinship analysis of distant relationships, specifically in cases involving identification of disaster victims or missing persons.
“Mobile Forensic Van” (MFV) (Big/Small) is being launched by Gujarat
Forensic Sciences University (GFSU) and Helik Advisory Ltd which helps
in reaching the crime scene at the earliest to assist the Investigating
Officers (IOs) in identifying and collecting relevant forensic evidence
from crime scene, victims & from suspects which are the primary
sources of forensic evidence. The Mobile Forensic Team assists the
Investigating Officer in linking the crime with the perpetrator through
the forensic evidence, collected from the crime scene, victims and
suspects.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
1. Examination of Disappearing Ink Writings
SUBMITTED BY:
Parth Chauhan
BSc. (H) Forensic Science VI Sem
A51605917024
2. Secret writing is the art of covered or hidden writing. The art of secret writing is intended
to make a message unreadable by at third party but does not hide the existence of the secret
communication. A new technique for committing document frauds using disappearing inks
in the execution of various bank instruments is currently very much in vogue. This study
deals with basic understanding about disappearing ink, different types of disappearing
inks, its compositions, laboratory preparation using different concentrations of chemical
compounds, comparative analysis between disappearing and erasable ink, Thermal ink as
disappearing ink, analysis fading time of different disappearing ink and decipherment of
the faded writing using physical and chemical methods and its consequence on the
evidences.
ABSTRACT
3. Secret Writing: Secret writing is any means of written communication whereby a spy conceals the
actual written text, whether it is enciphered or encoded or not. Dissapearing inks are used for
secret writing.
Disappearing ink: Inks that are visible for a period of time without the intention of being made
visible again are called disappearing inks.
Many criminals defraud banks with untrue identification, falsified, fabricated or altered cheques,
bank instruments, other documents and stolen financial information.
Criminals are using different types of erasures and disappearing inks to decolorize the writing for
fraudulent purposes.
INTRODUCTION
4. The alteration is one of the most common types of fraudulent observed in the
examination of questioned documents. It includes addition, subtraction, and obliteration
and to alter any security features on the documents(4)
Invisible inks can be classified into three main categories:
1. those that are revealed by heat,
2. those revealed by chemical reactions and
3. those that are visible under ultraviolet light.
It is very difficult for bank officials to detect such types of bank instruments written with
disappearing ink, as there are no signs of either physical or chemical erasure in the
aforesaid instruments and hence forgers typically achieve success in their goals. This
laboratory dealt with a case of this very nature involving a bank cheque and, prima facie,
it was difficult to detect any alteration or tampering in the said cheque. (3)
5. With the advancement of new research and technology, different types of pen having disappearing
inks are easily available in the market which are used by criminals for forgery.(2)
Literature survey revealed that mainly two types of inks are used in such pens-
(i) Thymolphthalein and Phenolphthalein based disappearing inks or its mixture,
(ii)Inks based on thermochromic substances disappearing due to changes in temperature (thermal
erasable ink).(2)
Some of the disappearing inks are water-based acid-base indicators (pH indicators), which change
from a coloured to a colourless solution upon exposure to air. The most common pH indicator for inks
is thymolphthalein (blue). This indicator is mixed with a basic solution that becomes more acidic
upon exposure to air, resulting in a change of colour.(5)
8. We prepare ink using Thymolphthalein and Phenolphthalein separately.
0.1 – 1.5 gm of Thymolphthalein or Phenolphthalein is dissolved in 60 mL of absolute ethyl
alcohol (laboratory grade) and solution is shaken until complete solubility.
After then, 6 mL of Glycerin (laboratory grade) added in the solution and shaken and 34 mL
water to make 100 mL volume of each ink solution.
0.5 – 4 mL of Sodium Hydroxide is then mixed keeping in mind the pH of the solution.
Desired ink is prepared which can be used by filling it in appropriate writing instrument.
Preparation of common disappearing inks(1)
9. Mohamed El-Molla Et. Al. in their research based on the experiment of different concentrations of
Thymolphthalein and Phenolphthalein ink and their mixtures on different types of papers were used
as described :
RESULT AND DISCUSSION
Papers Manufacturing company
White paper (80 g ⁄m²) Azhar (Al Husein International
Paper Industry, Giza, Egypt)
White paper (80 g ⁄m²) Xerox (Giza, Egypt)
White paper (80 g ⁄m²) Quena Paper Industry Company
(Kous, Quena, Egypt)
Edfo lined paper (60g ⁄m²) Edfu Misr Company
(Edfu, Asawan, Egypt)
Stencil printing paper (70
g ⁄m²)
Rakta Paper Manufacturing
Company (Abukeer Aalexandria,
Egypt)
PHENOLPHTHALEIN
in 60 mL Ethanol
THYMOLPHTHALEI
N in 60 mL Ethanol
0.1 gm(P1) 0.1 gm(T1)
0.2 gm(P2) 0.2 gm(T2)
0.4 gm(P3) 0.4 gm(T3)
0.8 gm(P4) 0.8 gm(T4)
1.2 gm(P5) 1.2 gm(T5)
10. Table 1: Effects of different conc. Of NaOH with different conc. Of Thymolphthalein on the fading time.
*Table 1 taken from El-Molla, M. M., Shama, S. A., & El-Sayed Saeed, S. (2012). Preparation of Disappearing Inks and Studying the
Fading Time on Different Paper Surfaces. Journal of Forensic Sciences, 58(1), 188–194*
11. Table 2: Effect of increase in volume of 1N NaOH with different conc. Of Thymolphthalein
Table 3 Effect of increase in volume of 1N NaOH with different conc. Of Thymolphthalein
*Table 2 & 3 taken from El-Molla, M. M., Shama, S. A., & El-Sayed Saeed, S. (2012). Preparation of Disappearing Inks and Studying the
Fading Time on Different Paper Surfaces. Journal of Forensic Sciences, 58(1), 188–194*
12. Table 4: Effects of different conc. Of NaOH with different conc. Of Thymolphthalein on the fading time.
*Table 4 taken from El-Molla, M. M., Shama, S. A., & El-Sayed Saeed, S. (2012). Preparation of Disappearing Inks and Studying the
Fading Time on Different Paper Surfaces. Journal of Forensic Sciences, 58(1), 188–194*
13. (1)Azhar x1000 (2)Xerox (x1000) (3)EDFO (x1000)
(4)Quena (x1000) (5)Rakta (x1000)
*Figure 1, 2, 3, 4 & 5 taken from El-Molla, M. M., Shama, S. A., & El-Sayed Saeed, S. (2012). Preparation of Disappearing Inks and Studying the Fading
Time on Different Paper Surfaces. Journal of Forensic Sciences, 58(1), 188–194*
14. The stability of the handwriting increased with increasing the concentration of NaOH with the
same surface, and low concentration of NaOH resulted in colorless writing. Also, the stability of
the handwriting increased with increasing the concentration of Thymolphthalein and
Phenolphthalein.
Azhar paper surfaces gave the more handwriting stability than Xerox, Quena, Edfo, and Rakta,
respectively.
At high volumes of 1 N NaOH with phenolphthalein, the stability of handwriting decreased as a
result of the formation of the colorless carbinol form.(Fig. F)
Fig. 6: Carbinol form mechanism
15. Later in 2012, Bikram Ashok Kumar Et. Al. in their research based on the experiment on
Thymolphthalein ink writings on different sample of cheques under VSC 2000.
Fig. 7. a) Photograph of a sample exhibit prepared using disappearing ink pen; b) photograph of secret writings
revealed below existing writings under a spotlight, a facility available in the VSC-2000 HR.
*Figure 7 (a) & (b) taken from Bikram Ashok Vaid, Sanjeev Kumar, Rajender Singh Rana, Narendra Kumar (2012).
Visualisation of disappearing ink writings. Problems of Forensic Sciences 2012, vol. 92, 311–318*
16. Fig. 8. a) Photograph of a bank instrument with existing writings submitted to this laboratory for verification of its authenticity; b)
photograph of secret writings revealed below existing writings under a spotlight, a facility available in the VSC-2000 HR.
Fig. 9 . a) Photograph of a bank instrument with existing writings submitted to this laboratory for verification of its authenticity; b)
photographs of secret writings revealed below the existing writings under a spotlight, a facility available in the VSC2000.
17. J(a) J(b)
J(c)
Fig 10. (a) Photograph of a bank instrument with existing writings submitted to this laboratory for verification of its authenticity;
b) photograph of secret writings revealed below existing writings under a spotlight, a facility available in the VSC-2000 ;
c) photographs of aforesaid instruments seen under transmitted light.
18. 11(b)
Fig 11. (a)Actual photographs of a bank instrument with writings and signatures executed with normal ink visible to the naked
eye; b) processed photographs of the same bank instrument as shown in Figure 11(a) above, revealing secret writings below the
existing writings using a spotlight in the VSC-2000 .
11(a)
19.
20. In 2013, Deepti Andharmule Et. Al. in their research based on the study of biological fluids(milk, saliva, and urine),
chemical fluids (vinegar, saline and baking soda) and vegetable fluids (lemon, orange and onion juice) and then
visualization by physical, chemical and by heat treatment with in the time interval of 30 days.
Table 5,6 & 7: Biological fluids, Vegetable fluids & chemical fluids visualized by physical
and chemical methods.
21. In 2017, Hussain SS and Sahu M. in their research based on the study of biological fluids(Milk, Saliva, and Sweat),
chemical fluids (Detergent, Sugar solution and Vinegar) and Fruit juices (Lemon, Apple and Grape juice) and then
visualization by physical and chemical treatment with in the time interval of 30 days.
Table 8,9 & 10 : Fruit juices, Chemical fluids & Biological fluids visualized by physical
and chemical methods.
22. In 2015, Vivek kumar Mangilal Chayal Et. Al. in their research based on decipherment of thermal ink on different
cheque papers.
Fig 12 (a) Sample of thermal ink in blue ink color; b) Photograph of the sample after applying heat on specific areas, original writings
disappeared and not visible; C) Sample of ghost strokes of disappeared writing of thermochromic thermal ink visible under UV 312 nm.
Fig.12(a) Fig.12(b)
Fig.12(c)
23. Fig 12 (d) Sample of rewritten/altered sample in blue thermochromic thermal ink; e) Ghost strokes of original disappeared
writing in altered/overwritten cheque visible under UV range 312 nm) in VSC-6000; f) Ghost strokes of original
disappeared writing in altered/overwritten cheque visible under UV range 365 nm in VSC-6000
Fig.12(d) Fig.12(e)
Fig.12(f)
24. Fig 12 (f) Ghost strokes of original disappeared writing and over written/altered writings visible under spot light in VSC-
6000; g) Reappeared original writing and over written/altered writing visible with the naked eye after cooling the sample
in a refrigerator at 0° to −10 °C.
Fig.12(f) Fig.12(g)
Refrigerator based method is destructive technique in nature as the samples will get exposed to humidity
and moisture inside the chamber.
The detection under VSC-6000 given better, result in terms of clarity compared to cooling and retrieving
under the freezing temperature in a refrigerator.
25. In 2017, examination conducted on thermal ink (Frixion ink) by Chee Hau Teo Et. Al. in Malaysia using
ESDA and VSC 6000: used three color of thermal ink – Blue, Black & Red
1. All three inks gave consistent result after heated and erased as fading could be observed by magnifying
glass but reading is not possible that indictaed presence of ink after fading.
2. Due to writing, indentation can be found which can be restored using ESDA and oblique light using VSC.
3. The rubbing action by eraser against the paper shows Pilling effect of paper which can be observed using
oblique light examination in VSC as shown in fig.13(a).
Fig 13 (a) Black Frixion ink removed by heating, then analyzed using ESDA; b) Blue Frixion ink after erasure showing
visible indentation and pilling on the surface of paper.
13(a) 13(b)
26. Thermal ink is the most commonly used ink among disappearing inks. The used is basically by cloth weavers
in factories and may be used in illegal way for committing property related crimes.
Available non- distructive methods for examination includes VSC and ESDA. VSC can be used with UV and
spotlight.
Available destructive method for examination includes Sodium hydroxide and ammonia solution.
Best method for the detection of thermal ink writings is Iodine fuming as it will not harm the paper and writing
and evaporated after some time.
VSC is much better technique for decipherment of thermal ink as compared to refrigeration technique which
may affects evidential value.
CONCLUSION
27. 1. El-Molla, M. M., Shama, S. A., & El-Sayed Saeed, S. (2012). Preparation of Disappearing
Inks and Studying the Fading Time on Different Paper Surfaces. Journal of Forensic Sciences,
58(1), 188–194.
2. Chayal, V. M., Handa, D. R., Singh, J., & Menon, S. K. (2015). “A Sensitive Non-destructive
Method for Detection of Document Frauds using Thermal Ink.” Australian Journal of Forensic
Sciences, 48(5), 601–612.
3. Welch, J. (2008). Erasable ink; something old, something new. Science & Justice, 48(4), 187–
191.
4. Deshpande Hemantini, Mulani Khudbudin(2018). Decipherment of disappearing ink: A case
study, Brazilian Journal of Forensic Sciences, Medical Law and Bioethics 7(3):156-161
REFERENCES
28. 5. Bikram Ashok Vaid, Sanjeev Kumar, Rajender Singh Rana, Narendra Kumar (2012).
Visualisation of disappearing ink writings. Problems of Forensic Sciences 2012, vol. 92, 311–318
6. Deepti Andharmule, Neeti Kapoor, Ashish Badiye (2013). An Evaluation of Some commonly
used Methods for Visualization of Secret Writing. Research Journal of Forensic Sciences, Vol. 1(3),
2321–1792
7. Manal Abd-El, Aziz Abd-El Zaher (2014). Different types of inks having certain medicolegal
importance: Deciphering the faded and physically erased handwriting. Egyptian Journal of
Forensic Sciences (2014) vol. 4, 39–44
8. Asicioglu, F., Tekin, T., Ozbek, N., Cevik, F. E., Ozcan, M., & Mohammed, L. A.
(2019). Prepared Disappearing Ink and Deciphering of Documents. Journal of Forensic Sciences.
9. Hussain SS, Sahu M (2017). A Study on Common Visualization Methods for Secret Writing done
by Invisible Inks and their Sensitivity Over a Period of Time. Austin J Forensic Sci Criminol. 2017;
4(2): 1061
10. Teo, C. H., Mohamad Noor, S. N. M., & Wong, K. Y. (2017). Ink that disappears: examination
of questioned documents related to Frixion ink in Malaysia. Canadian Society of Forensic Science
Journal, 50(3), 146–155.
Once you find your sources, you will want to evaluate your sources using the following questions:
Author:
Who is the author?
Why should I believe what he or she has to say on the topic?
Is the author seen as an expert on the topic? How do you know?
Current:
How current is the information in the source?
When was the source published?
Is the information out-of-date?
Accuracy:
Is the content accurate?
Is the information presented objectively? Do they share the pros and cons?
Once you find your sources, you will want to evaluate your sources using the following questions:
Author:
Who is the author?
Why should I believe what he or she has to say on the topic?
Is the author seen as an expert on the topic? How do you know?
Current:
How current is the information in the source?
When was the source published?
Is the information out-of-date?
Accuracy:
Is the content accurate?
Is the information presented objectively? Do they share the pros and cons?
Once you find your sources, you will want to evaluate your sources using the following questions:
Author:
Who is the author?
Why should I believe what he or she has to say on the topic?
Is the author seen as an expert on the topic? How do you know?
Current:
How current is the information in the source?
When was the source published?
Is the information out-of-date?
Accuracy:
Is the content accurate?
Is the information presented objectively? Do they share the pros and cons?
Once you find your sources, you will want to evaluate your sources using the following questions:
Author:
Who is the author?
Why should I believe what he or she has to say on the topic?
Is the author seen as an expert on the topic? How do you know?
Current:
How current is the information in the source?
When was the source published?
Is the information out-of-date?
Accuracy:
Is the content accurate?
Is the information presented objectively? Do they share the pros and cons?
Once you find your sources, you will want to evaluate your sources using the following questions:
Author:
Who is the author?
Why should I believe what he or she has to say on the topic?
Is the author seen as an expert on the topic? How do you know?
Current:
How current is the information in the source?
When was the source published?
Is the information out-of-date?
Accuracy:
Is the content accurate?
Is the information presented objectively? Do they share the pros and cons?
Once you find your sources, you will want to evaluate your sources using the following questions:
Author:
Who is the author?
Why should I believe what he or she has to say on the topic?
Is the author seen as an expert on the topic? How do you know?
Current:
How current is the information in the source?
When was the source published?
Is the information out-of-date?
Accuracy:
Is the content accurate?
Is the information presented objectively? Do they share the pros and cons?
Once you find your sources, you will want to evaluate your sources using the following questions:
Author:
Who is the author?
Why should I believe what he or she has to say on the topic?
Is the author seen as an expert on the topic? How do you know?
Current:
How current is the information in the source?
When was the source published?
Is the information out-of-date?
Accuracy:
Is the content accurate?
Is the information presented objectively? Do they share the pros and cons?
Once you find your sources, you will want to evaluate your sources using the following questions:
Author:
Who is the author?
Why should I believe what he or she has to say on the topic?
Is the author seen as an expert on the topic? How do you know?
Current:
How current is the information in the source?
When was the source published?
Is the information out-of-date?
Accuracy:
Is the content accurate?
Is the information presented objectively? Do they share the pros and cons?
After consulting a variety of sources, you will need to narrow your topic. For example, the topic of internet safety is huge, but you could narrow that topic to include internet safety in regards to social media apps that teenagers are using heavily. A topic like that is more specific and will be relevant to your peers. Some questions to think about to help you narrow your topic:
What topics of the research interest me the most?
What topics of the research will interest my audience the most?
What topics will the audience find more engaging? Shocking? Inspiring?