This document discusses a study on the effects of hydatid disease caused by the parasite Echinococcus granulosus on lipid profiles in infected patients. The study found significantly increased levels of cholesterol, triglycerides, LDL, VLDL, and immunoglobulin G, as well as decreased levels of HDL, in 30 patients with hydatid disease compared to 20 healthy patients. This lipid profile change is likely due to the body's acute phase response to the parasitic infection and resulting inflammation.

2.  THE HYDATID DISEASE IS AN INFECTION
CAUSED BY THE LARVAL STAGES OF
“ECHINOCOCCUS GRANULOSA”
 LIFE CYLE IN TWO HOSTS- A CARNIVOROUS
DEFINITIVE HOST AND INTERMEDIATE HOST.
 THE EGGS PENETRATE THE GUT MUCOSA AND
ENTER THE BLOOD STREAM FROM WHICH
THEY ARE DISTRIBUTED TO VARIOUS SITES OF
BODY.

3.  THE SYMPTOMS AND SIGNS THAT OCCUR
DEPENDS ON THE CYSTS LOCATION AND SIZE.
 AIM OF THIS EXPERIMENT IS TO STUDY THE
MEASUREMENT OF EFFECTED INFECTION BY
HYDATID CYSTS ON LIPID PROFILES IN
INFECTED BODY.

4.  30 SAMPLES WERE COLLECTED FROM THE
AFFECTED PATIENTS WITH HYDATID DISEASE
AND 20 SAMPLES FROM HEALTHY PATIENTS.
 THE SAMPLES WERE KEPT IN SPECIMEN TUBES
AND REMAIN FOR 30 MIN AT ROOM
TEMPERATURE.
 AFTER THEY WERE CENTRIFUGATED(TO
SEPARATE SERUM AND COLLECTED IN OTHER
STERILE TUBES).

5.  THE SAMPLES ARE KEPT IN DEEP FREEZE TILL
THEY ARE USED FOR THE DETERMINATION OF
LIPID PROFILE AND IMMUNOGLOBULIN G
CONCENTRATION.
 LIPID PROFILE INCLUDES
1. CHOLESTEROL.
2. TRIGLYCERIDE.
3. LDL.
4. HDL.
5. VLDL.

6. MEASUREMENT OF LIPIDS:
1.ESTIMATION OF TOTAL SERUM CHOLESTEROL.
ESTIMATION OF TOTAL SERUM TRIGLYCERIDES.
2. MEASUREMENT OF TG’S IN THE SERUM BY
USING ENZYMATIC AND COLORIMETRIC
METHOD.
3. ESTIMATION OF TOTAL SERUM HDL.
4.VLDL CHOLESTEROL(mg/100ml)=TG’S.
5.LDL=TC-HDL-TG.

7. 4. MEASUREMENT OF IMMUNOGLOBULIN G IN THE
SERUM – SINGLE RADIAL DIFFUSION METHOD.
THE PRESENT STUDY SHOWS –
 THE SIGNIFICANT INCREASE IN:
 LEVEL OF CHOLESTEROL.
 LEVEL OF TRIGLYCERIDES.
 LEVEL OF VLDL AND LDL.
 LEVEL OF IMMUNOGLOBULIN G.
 AND THE DECREASE IN HDL LEVEL.
IN HYDATID DISEASE PATIENTS COMPARED TO
HEALTHY PERSONS(CONTROL GROUP).
RESULTS:

8. CHOLESTEROL 214.55 mg/dl 198.4 mg/dl
TRIGLYCERIDES 113.9 mg/dl 73.6 mg/dl
LDL 139.62 mg/dl
85.93 mg/dl
VLDL 40.29 mg/dl 39.18 mg/dl
HDL 38.8 mg/dl 45.23 mg/dl
IMMUNOGLOBULIN IG g 2492 mg/dl 1190 mg/dl

9.  IT WAS A FACT THAT THAT THE INFECTION AND
INFLAMMATION PRODUCE MODERATE CHANGES IN
PLASMA LIPOPROTEIN PATTERN IN VARIETY OF
INFLAMMATORY DISORDERS.
 THIS IS DUE TO ACUTE PHASE RESPONSE (APR)
LEADING TO MULTIPLE ALTERATIONS IN LIPID AND
LIPOPROTEIN METABOLISM.
 MOST OF THE STUDIES SHOWS THAT THIS IS DUE
TO PARASITIC INFECTIONS LEVELS OF
LIPOPROTEINS.

10.  ECHINOCOCCUS ORGANISMS ARE HIGHLY
IMMUNOGENIC , STIMULATES HIGH
INFLAMMATORY CELL RESPONSES , T-CELL AND
OTHER MEDIATED RESPONSES IN HUMANS AND
INTERCELLULAR HOSTS.
 THIS STUDY HAS ALSO PROVEN BY SOME
RESEARCHES WHO HAVE SHOWN SIGNIFICANT
INCREASE IN IG-g PRODUCTION IN MICE AFTER
INJECTION OF HYDATID FLUID.

12.  SALEEM.K.A ,ALHADRAWY,MAYSOON,ALHADRAWRY, ALI
HASSAN- ABOOD,KHALFAH HUSSAIN.
 DEPARTMENT OF BIOLOGY ,FACULTY OF SCIENCE,UNIVERSITY
OF KULFA,IRAQ.
ASIAN JOURNAL OF MICROBIAL,BIOTECH,ENV.SC, VOLUME-18.
THANK U