This study investigated CD36 gene status in north Indian subjects with type 2 diabetes (T2DM) by screening for deletions of exons 3, 4 and 5 and certain polymorphisms. Blood samples were taken from 300 T2DM patients and 100 healthy controls. Biochemical parameters and DNA extraction were performed, and exons 3-5 were analyzed via PCR. Deletions of these exons and certain polymorphisms were associated with T2DM. The study aims to further analyze genetic factors and deletions related to the CD36 gene in T2DM patients in north India.

1. Preliminary studies on CD36 in type 2 diabetic patients from north India Sunaina Gautam, C. G. Agrawal, Hemant Kumar Bid & Monisha Banerjee July 2011 María Isabel Henao Montaño Luis Carlos Fuentes Acosta III Semestre Medicina UPB Agosto 26 de 2011

8. Relationship between CD36 and DM2 The CD36 is a protein responsible for the endocytosis of LDL and if it’s faulty will not be able to enter the LDL. Therefore quickly the macrophages would transform them into foam cells which would generate an increase of free fatty acids. This increase will generate an insulin resistance and then it would generate a mellitus diabetes type 2.

9. GENERAL OBJECTIVE We undertook this study to investigate CD36 gene status in north Indian subjects by screening for the deletion of exons 3, 4 and 5 and certain polymorphisms.

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24. DISCUSSION AUTOR COMMENTS AGREE / DESAGREE (32) Febbraio M, Hajjar DP, Silverstein RL. (33) Furuhashi M, Ura N, Nakata T, Shimamoto K. “ Studies have shown its involvement in diverse disorders such as insuline resistance, dyslipidaemia, hyperlipidaemia, atherosclerosis” (15) Handberg A, Levin K, Hojlund K, et al. “ CD36 expression in monocytes is upregulated by oxidized low density lipoprotein (Ox-LDL), whose levels increase in case of T2DM, hyperglicaemia and related atherosclerosis”

25. AUTOR COMMENTS AGREE / DESAGREE (13) Miyaoca K Kuwasako T, et al. (35) Imai M, Tanaka T, et al. (36) Kintaka T, Tanaka T, et al. “ CD36 genotype was identified as a fundamental determinant of myocardial long chain fatty-acid uptake” (37) Banerjee M, Gautam S, Saxena M, Bid HK, Agrawal CG. “ We have recently reported that one of the several SNPs in the CD36 gene (rs1761667, G>A) shows a significant association with T2DM in the north Indian population.”

26. The conclusions of these studies lead to a guide of genotypic of CD36 and its associations with T2DM. These studies also have shown that the results are backed up in different multi-ethnical and genotipical analysis focused on the specific mutation related to CD36 gen. CONCLUSSIONS

27. CD36, associated with metabolic and hypertense syndromes, is encoded by different gens that despite being on the same chromosome, do not have the nucleotidal identical sequences, that means that there is no sustitution or replace due damaged or deficiency of one of them. Therefore, from the begin of the protein codification exists a damaged sequence, provoking its inactivity, leading to the appearance of diferent diseases.

28. Most of the studied cases in north india shown that a possible relations between the high rating of people affected by T2DM and de deletion and deficiency of CD36 gen can exist. deletion of exons 3, 4 and 5 and certain polymorphisms lead to investigators to further studies about genetical factors and deletion related to CD36 gen.

29. This study confirmed that the ages are not relevant because they can relate to young adults from 10 to 50 years so we conclude that CD36 is a problem of deficiency in any human in this case the population of northern India.

30. MAPA CONCEPTUAL Maria Isabel Henao

31. MAPA CONCEPTUAL Luis Carlos Fuentes