Enamel and Amelogenesis
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Enamel is the hardest tissue in the body. It is formed by cells called ameloblasts secreting enamel matrix proteins. Enamel cannot renew itself because the ameloblasts are only present during tooth development. Enamel acquires a complex structural organization and high mineralization to compensate for this limitation. Enamel has a crystalline structure composed mainly of calcium and phosphate ions. It contains enamel rods that run from the dentin-enamel junction to the enamel surface. Enamel formation involves two stages - initial mineralization followed by maturation where the crystals grow in size.
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Enamel and Amelogenesis
- 2. Dr.Shubhangi
- 3. HARDEST TISSUE OF BODY formed by AMELOBLASTS enamel Dr.Shubhangi
- 4. Ameloblasts Forming enamel Forming pulp cavity Odontoblasts Forming dentin Cervical loop Cervical loop Dr.Shubhangi Dr.Shubhangi
- 5. CANNOT RENEW Because AMELOBLASTS during tooth eruption enamel Dr.Shubhangi
- 6. …To COMPENSATE this inherent LIMITATION, Acquires a COMPLEX STRUCTURAL ORGANIZATION & HIGH DEGREE OF MINERALISATION (by almost total absence of in its mature state) enamel Dr.Shubhangi
- 7. PHYSICAL AND CHEMICAL CHARACTERISTICS enamel Dr.Shubhangi
- 8. • LIGHT YELLOW TO GRAY WHITE • THICKNESS MAXIMUM 2.5 mm (working surfaces) MINIMUM: FEATHER EDGE (cervical line) Dr.Shubhangi
- 9. Dr.Shubhangi
- 10. ENAMEL Crystalline Calcium phosphate Ions: Carbonate, strontium, magnesium, lead, fluoride 4% Proteins Lipids Dr.Shubhangi
- 12. AN IMAGE from SCANNING ELECTRON MICROSCOPE ENAMEL DENTIN PULP Dr.Shubhangi
- 14. ENAMEL RODS (prisms) INTERROD ENAMEL (interprismatic substance) Dr.Shubhangi
- 15. ENAMEL RODS (prisms) INTERROD ENAMEL (interprismatic substance) Dr.Shubhangi
- 16. ENAMEL RODS (prisms) INTERROD ENAMEL (interprismatic substance)
- 17. ENAMEL RODS (prisms) INTERROD ENAMEL (interprismatic substance) Dr.Shubhangi
- 18. ENAMEL RODS (prisms) INTERROD ENAMEL (interprismatic substance) Dr.Shubhangi
- 19. ENAMEL RODS (prisms) INTERROD ENAMEL (interprismatic substance) ENAMEL CRYSTALS Dr.Shubhangi
- 20. Note the different ORIENTATION of the CRYSTALS Dr.Shubhangi
- 21. Note the different ORIENTATION of the CRYSTALS Dr.Shubhangi
- 25. Do you remember ? Inner Enamel Epithelium Dr.Shubhangi
- 27. Do you remember in Advanced Bell Stage ? Inner Enamel Epithelium Dr.Shubhangi
- 28. Advanced Bell Stage Odontoblasts Thin layer of dentin Inner Enamel Epithelium We secreted some dentinal matrix Now, you START secreting ENAMEL matrix OKAY! We will change into TALL ameloblasts now! Dr.Shubhangi
- 29. Advanced Bell Stage Odontoblasts Thin layer of dentin AMELOBLASTS Just like we did. At the CUSP first! Then move downwards to cervical area! But, from where should we start depositing ENAMEL MATRIX ? Dr.Shubhangi
- 31. Odontoblasts dentin AMELOBLASTS Mineralized Enamel Our source of NUTRITION was dental papilla YES! And this enamel and dentin has made us move away from dental papilla Where to get nutrition from? Dr.Shubhangi
- 33. Odontoblasts dentin AMELOBLASTS Mineralized Enamel Our source of NUTRITION was NOW, our source of nutrition is! Thanks to the COLLAPSED outer enamel epithelium! Dental papilla Dental sac Dr.Shubhangi
- 34. Odontoblasts Thin layer of dentin ameloblasts ( ) Inner Enamel Epithelium Odontoblasts dentin AMELOBLASTS Mineralized Enamel Odontoblasts dentin AMELOBLASTS Mineralized Enamel Enamel MINERALIZATION Dr.Shubhangi
- 36. I will interact with adjacent mesenchymal cells and determine the shape of DEJ and crown Dr.Shubhangi
- 37. I will interact with adjacent mesenchymal cells and determine the shape of DEJ and crown Dr.Shubhangi
- 38. I will keep interacting with adjacent mesenchymal cells and feed them with some proteins (like enamel matrix), till they agree to become Odontoblasts! Dr.Shubhangi
- 39. I will keep interacting with adjacent mesenchymal cells and feed them with some proteins (like enamel matrix), till they agree to become Odontoblasts! Dr.Shubhangi
- 40. Odontoblasts got convinced and secreted some dentin. NOW I FEEL MOTIVATED to secrete . Dr.Shubhangi
- 41. Odontoblasts got convinced and secreted some dentin. NOW I FEEL MOTIVATED to secrete . Dr.Shubhangi
- 42. Most of the thickness of enamel matrix has been formed. I will help in maturation of enamel by ABSORBING some things. Dr.Shubhangi
- 43. Most of the thickness of enamel matrix has been formed. I will help in maturation of enamel by ABSORBING some organic matrix. Dr.Shubhangi
- 44. Most of the thickness of enamel matrix has been formed. I will help in maturation of enamel by ABSORBING some organic matrix. Dr.Shubhangi
- 45. My job is done. I will just sit reduce and Protect the fully mineralized enamel. Dr.Shubhangi
- 46. I have to lyse the overlying connective tissue, to help the tooth erupt in oral cavity. Dr.Shubhangi
- 47. Dr.Shubhangi
- 48. 2 steps: AMELOBLASTS secrete matrix proteins Matrix and water is lost CRYSTALS grow wider and thicker enamel enamel immature mature Dr.Shubhangi
- 50. Odontoblasts Thin layer of dentin ameloblasts ( ) Inner Enamel Epithelium Odontoblasts Dr.Shubhangi Odontoblasts Thin layer of dentin ameloblasts Dr.Shubhangi Dr.Shubhangi
- 51. Dr.Shubhangi
- 52. Development of Tomes’ processes Dr.Shubhangi
- 54. Dr.Shubhangi
- 56. Smooth ended ameloblasts Ruffle ended ameloblasts Dr.Shubhangi
- 57. Dr.Shubhangi
- 58. MINERALISATION of enamel Step 2: Dr.Shubhangi
- 59. Two stages: Immediate PARTIAL mineralization MATURATION Dr.Shubhangi
- 60. Immediate PARTIAL mineralization Odontoblasts Thin layer of dentin Inner Enamel Epithelium Now our time to help you. Take some APATITE and get mineralized! Thankyou for helping us with NUCLEATION! Dr.Shubhangi Dr.Shubhangi
- 61. Odontoblasts dentin AMELOBLASTS Mineralized Enamel Immediate PARTIAL mineralization Dr.Shubhangi
- 62. MATURATION Odontoblasts dentin AMELOBLASTS Mineralized Enamel Enamel MINERALIZATION Dr.Shubhangi
- 63. AMELOGENESIS Dr.Shubhangi *4 µ/ day Faster in permanent than in deciduous teeth
- 68. Dr.Shubhangi
- 77. Hunter Schreger bands Best seen in REFLECTED LIGHT DIAZONES PARAZONES Dr.Shubhangi
- 78. Hunter Schreger bands Reverse appearance seen in TRANSMITTED LIGHT DIAZONES PARAZONES Dr.Shubhangi
- 79. What is transmitted and reflected light? Dr.Shubhangi