Electron transport and oxidative phosphorylation are critical activities of protein complexes in the inner mitochondrial membrane, which ultimately serve as the major source of cellular energy. Several protein complexes in the electron transport chain function as proton pumps, using the energy of the electron to pump protons into the intermembrane space of the mitochondria. In the process of oxidative phosphorylation, electrons obtained from NADH and FADH2 combine with O2, and the energy released from these oxidation and reduction reactions is used to drive the synthesis of ATP from ADP. The transfer of electrons from NADH to O2 is a very high energy-yielding reaction. To be utilized in usable form, this energy must be produced gradually, by the passage of electrons through a series of transporters, which constitute the electron transport chain. These transporters are organized into four complexes in the inner mitochondrial membrane. A fifth protein complex then serves to couple the energy-yielding reactions of electron transport to ATP synthesis. Solution Electron transport and oxidative phosphorylation are critical activities of protein complexes in the inner mitochondrial membrane, which ultimately serve as the major source of cellular energy. Several protein complexes in the electron transport chain function as proton pumps, using the energy of the electron to pump protons into the intermembrane space of the mitochondria. In the process of oxidative phosphorylation, electrons obtained from NADH and FADH2 combine with O2, and the energy released from these oxidation and reduction reactions is used to drive the synthesis of ATP from ADP. The transfer of electrons from NADH to O2 is a very high energy-yielding reaction. To be utilized in usable form, this energy must be produced gradually, by the passage of electrons through a series of transporters, which constitute the electron transport chain. These transporters are organized into four complexes in the inner mitochondrial membrane. A fifth protein complex then serves to couple the energy-yielding reactions of electron transport to ATP synthesis..