This study investigated whether treating Cupressus tissue cultures with methyl jasmonate would enhance the cultures' production of the antifungal compound β-thujaplicin. Cultures of Cupressus dupreziana callus tissue were transferred to fresh media containing either methyl jasmonate or a control solution. Gas chromatography-mass spectrometry analysis found that the cultures produced β-thujaplicin but its levels did not increase when treated with methyl jasmonate. This suggests that methyl jasmonate does not enhance β-thujaplicin production in these callus cultures.