1. CURRICULUM VITAE
PERSONAL INFORMATION
Name Federico Sabbadin
Address 2 Wellington Street, YO10 5BD, York, UK
Telephone Personal: (44) 01904629363
Mobile: (44) 07799116030
E-mail fs525@york.ac.uk
Nationality Italian
Date of birth 7 March 1983
Gender Male
WORK EXPERIENCE
Dates October 2007 – present
Name and address of employer Centre for Novel Agricultural Products (CNAP), Department of Biology,
University of York, United Kingdom
York, YO10 5YW
(44) 01904328783
Position held PhD Student
Main activities/skills covered
- designing a platform for high-throughput generation of libraries of
chimeric P450s for biocatalysis and characterization of orphan
P450s
- developing new high throughput methods to screen the activity of
NAD(P)H-dependent enzymes over panels of potential substrates
- X-ray crystallography of XplA, an explosive degrading P450
- site-directed mutagenesis of XplA, characterization and kinetic
analysis of the mutants
- directed evolution of XplA P450 towards the degradation of the
explosive pollutant HMX
- techniques: PCR, gene cloning, DNA primer design and in silico
DNA analysis, whole cell and in vitro activity assays, protein
expression in Escherichia coli host, purification via metal affinity
chromatography and gel filtration chromatography, SDS-PAGE,
Western Blot, FPLC, HPLC, GC-MS, protein crystallization
techniques (vapour diffusion), enzyme kinetics

2. Dates October 2006 – July 2007
Name and address of employer Agripolis Campus, Viale dell`Universita` 16 -35020- Legnaro,
Padova, Italy
(39) 049 827 25 35
Position held Master`s Degree Student
Main activities/skills covered - site directed mutagenesis of the polygalacturonase enzyme
produced by the pathogenic fungus Fusarium verticilllioides
- activity analysis of the poly-galacturonase mutants
- techniques: DNA primer design, PCR, site directed mutagenesis,
FPLC, isoelectric facousing, Escherichia coli and Pichia pastoris
transformation (heat shock and PEG techniques), expression of
heterologous proteins in the methylotrophic yeast Pichia pastoris,
bacterial and yeast culture
Dates May – July 2005
Name and address of employer Agripolis Campus, Viale dell`Universita` 16 -35020- Legnaro,
Padova, Italy
(39) 049 827 25 35
Position held Trainee
Main activities/skills covered - immunolocalization of AtPIN1 auxin carrier in maize plant
- in-situ hybridization of AtPIN1 auxin carrier in maize plant
- radioactive Northern Blotting of AtPIN1 auxin carrier mRNA
REFEREED PAPERS
1. Sabbadin, F., Hyde, R., Robin, A., Hilgarth, E., Delenne, M., Flitsch,
S., Turner, N., Grogan, G., and Bruce, N. C., 2010. LICRED: a
versatile drop-in vector for rapid generation of redox-self-sufficient
cytochrome P450s. ChemBioChem (accepted, in press).
2. Sabbadin, F., Jackson, R., Haider, K., Tampi, G., Turkenburg, J. P.,
Hart, S., Bruce, N. C., and Grogan, G., 2009. The 1.5-Å structure of
XplA-heme, an unusual cytochrome P450 heme domain that catalyzes
reductive biotransformation of the Royal Demolition Explosive. J. Biol.
Chem., 284: 28467-28475.
3. Robin, A., Roberts., G. A., Kisch, J., Sabbadin, F., Grogan, G.,
Bruce, N. C., Turner, N. J., and Flitsch, S. L., 2009. Engineering and
improvement of the efficiency of a chimeric [P450cam-RhFRed
reductase domain] enzyme. Chem. Comm., 18: 2478-2480.

3. AWARDS
Best Poster Award. 16th International Conference on Cytochrome
P450, 2009. Nago, Okinawa, Japan.
EDUCATION AND TRAINING
Dates October 2005 – July 2007
Name and type of organisation University of Padova, Padova, Italy
providing education and training
Principal subjects/occupational - plant biology, physiology, ecology, pathology
skills covered
- molecular biology, genetics
- standard laboratory chemical techniques
- plasmidic DNA extraction, purification and quantification
- DNA primer design, PCR amplification, sequencing, aligning
Title of qualification awarded Master’s Degree in Nutrition Biotechnologies
Level in national classification ISCED 5
Vote obtained 110/110 with honors
Dates October 2002 – October 2005
Name and type of organisation University of Padova, Padova, Italy
providing education and training
Principal subjects/occupational - plant biology, physiology, ecology, pathology
skills covered
- molecular biology, genetics
Title of qualification awarded Bachelor of Science in Agriculture Biotechnologies
Level in national classification ISCED 5
Vote obtained 110/110 with honors
Dates September 1997 – July 2002
Name and type of organisation Scientific Liceum “E. Curiel”, via Durer 14 -35132- Padova, Italy
providing education and training
Principal subjects/occupational - biology
skills covered
- mathematics
- physics
- chemistry
Title of qualification awarded Diploma
Level of national classification ISCED 3
Vote obtained 100/100 with honors

4. PERSONAL SKILLS
AND COMPETENCES
MOTHER LANGUAGE Italian
OTHER LANGUAGES
English
Reading skills excellent
Writing skills excellent
Verbal skills excellent
French
Reading skills good
Writing skills good
Verbal skills good
SOCIAL SKILLS Team work: I have worked as a pizza chef for 5 months, collaborating
with the restaurant personnel. I have also practiced team sports
(basket, volleyball)
TECHNICAL SKILLS AND Competent with most Microsoft Office programmes
COMPETENCES
OTHER SKILLS AND - certified PADI Open Water Diver
COMPETENCES
- athletics and fitness
- amateur home microscopy
DRIVING LICENCE(S) Cathegory B vehicle